################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AgeI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BglI BlnI BlpI Bpu1102I BsaAI BsaI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII Bst1107I BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI FseI FspI HincII HindII HpaI KasI KpnI KspI MluI MluNI MroI MscI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcyI 455 GGCGTC G[GA]^CG[CT]C AflII 1873 CTTAAG C^TTAAG AflIII 414 ACACGT A^C[GA][CT]GT Alw44I 350 GTGCAC G^TGCAC ApaLI 350 GTGCAC G^TGCAC AseI 1848 ATTAAT AT^TAAT AsnI 1848 ATTAAT AT^TAAT AspI 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 28 GCTGAGG CCTCAGC(-5/-2) BcgI 747 GCAAGTTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1162 GGATAC GTATCC(6/5) BclI 470 TGATCA T^GATCA BfrI 1873 CTTAAG C^TTAAG BsaHI 455 GGCGTC G[GA]^CG[CT]C BsaWI 1880 ACCGGA [AT]^CCGG[AT] BsgI 1271 CTGCAC GTGCAG(16/14) BsiYI 2686 CCTGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 2686 CCTGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 457 CGTCTC CGTCTC(1/5) BsmFI 1238 GGGAC GGGAC(10/14) BspHI 2789 TCATGA T^CATGA BsrFI 2480 ACCGGC [GA]^CCGG[CT] BstBI 2321 TTCGAA TT^CGAA BstEII 2565 GGTCACC G^GT[ACGT]ACC BstPI 2565 GGTCACC G^GT[ACGT]ACC CfoI 123 GCGC GCG^C Cfr10I 2480 ACCGGC [GA]^CCGG[CT] EaeI 2045 TGGCCG [CT]^GGCC[GA] EcoNI 2686 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2565 GGTCACC G^GT[ACGT]ACC EcoRV 711 GATATC GAT^ATC EcoT22I 367 ATGCAT ATGCA^T FbaI 470 TGATCA T^GATCA HaeII 122 GGCGCT [GA]GCGC^[CT] HhaI 123 GCGC GCG^C Hin1I 455 GGCGTC G[GA]^CG[CT]C HinP1I 123 GCGC G^CGC MfeI 2238 CAATTG C^AATTG MunI 2238 CAATTG C^AATTG NsiI 367 ATGCAT ATGCA^T NspV 2321 TTCGAA TT^CGAA PflFI 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1743 AGGACCC [GA]G^G[AT]CC[CT] PshBI 1848 ATTAAT AT^TAAT RcaI 2789 TCATGA T^CATGA SfuI 2321 TTCGAA TT^CGAA SmaI 1235 CCCGGG CCC^GGG SmlI 1873 CTTAAG C^T[CT][GA]AG SphI 1426 GCATGC GCATG^C Tth111I 2373 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1235 CCCGGG C^CCGGG XmaI 1235 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AvaI 1235 CCCGGG C^[CT]CG[GA]G 2429 CCCGAG BbsI 621 GAAGAC GAAGAC(2/6) 2476 GAAGAC BglII 1548 AGATCT A^GATCT 2856 AGATCT BpuAI 621 GAAGAC GAAGAC(2/6) 2476 GAAGAC BseRI 1501 GAGGAG GAGGAG(10/8) 2737 GAGGAG BsoBI 1235 CCCGGG C^[CT]CG[GA]G 2429 CCCGAG BssSI 1409 CACGAG CACGAG(-5/-1) 1531 CACGAG BstAPI 678 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1732 GCAGCTGCTGC DraI 385 TTTAAA TTT^AAA 2013 TTTAAA DraII 1743 AGGACCC [GA]G^G[ACGT]CC[CT] 2847 AGGCCCT DsaI 764 CCATGG C^C[GA][CT]GG 1761 CCATGG EcoO109I 1743 AGGACCC [GA]G^G[ACGT]CC[CT] 2847 AGGCCCT HgaI 456 GCGTC GACGC(5/10) 867 GCGTC HindIII 598 AAGCTT A^AGCTT 2492 AAGCTT MaeII 416 ACGT A^CGT 1209 ACGT NcoI 764 CCATGG C^CATGG 1761 CCATGG SapI 2324 GAAGAGC GCTCTTC(1/4) 2746 GAAGAGC SpeI 186 ACTAGT A^CTAGT 1279 ACTAGT ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaBI BsaJI BsiHKAI BsmAI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII