(Circular) (Six-base) MAPSORT of: pFN21AA0898.seq Check: 8452 from: 1 to: 7597 pFN21AA0898 7597 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3937 3937 Size: 7597 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5407 278 Size: 53 83 186 936 3871 2468 Fragments arranged by size: 3871 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4940 1937 Size: 3003 4594 Acc65I G'GTAC_C Cuts at: 4918 4918 Size: 7597 AclI AA'CG_TT Cuts at: 1563 5724 6097 1563 Size: 4161 373 3063 Fragments arranged by size: 4161 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 2654 4407 4500 4665 4692 4713 4878 Size: 983 39 1753 93 165 27 21 165 Cuts at: 4878 5660 7044 7312 1632 Size: 782 1384 268 1917 Fragments arranged by size: 1917 1753 1384 983 782 268 165 165 93 39 27 21 AflII C'TTAA_G Cuts at: 820 1017 3878 820 Size: 197 2861 4539 Fragments arranged by size: 4539 2861 197 AflIII A'CryG_T Cuts at: 1203 2419 6496 7473 1203 Size: 1216 4077 977 1327 Fragments arranged by size: 4077 1327 1216 977 AgeI A'CCGG_T Cuts at: 6428 6428 Size: 7597 AhdI GACnn_n'nnGTC Cuts at: 6326 6326 Size: 7597 AleI CACnn'nnGTG Cuts at: 1166 2083 2429 1166 Size: 917 346 6334 Fragments arranged by size: 6334 917 346 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7597 AlwNI CAG_nnn'CTG Cuts at: 2888 3473 3743 4381 4514 5340 6912 2888 Size: 585 270 638 133 826 1572 3573 Fragments arranged by size: 3573 1572 826 638 585 270 133 ApaLI G'TGCA_C Cuts at: 2355 5654 6810 2355 Size: 3299 1156 3142 Fragments arranged by size: 3299 3142 1156 ApoI r'AATT_y Cuts at: 1493 2406 2857 3089 3901 4906 5132 5284 7393 Size: 913 451 232 812 1005 226 152 2109 Cuts at: 7393 1493 Size: 1697 Fragments arranged by size: 2109 1697 1005 913 812 451 232 226 152 AseI AT'TA_AT Cuts at: 160 1032 3854 6151 160 Size: 872 2822 2297 1606 Fragments arranged by size: 2822 2297 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7597 AvaI C'yCGr_G Cuts at: 1870 1943 3240 4434 4922 1870 Size: 73 1297 1194 488 4545 Fragments arranged by size: 4545 1297 1194 488 73 BamHI G'GATC_C Cuts at: 4927 5294 4927 Size: 367 7230 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4918 6373 618 Size: 325 217 627 3131 1455 1842 Fragments arranged by size: 3131 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2152 2729 2997 4916 1391 Size: 761 577 268 1919 4072 Fragments arranged by size: 4072 1919 761 577 268 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2633 4488 928 Size: 971 734 1855 4037 Fragments arranged by size: 4037 1855 971 734 BbvCI CC'TCA_GC Cuts at: 2034 2034 Size: 7597 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2741 2775 5788 5822 1903 Size: 34 804 34 3013 34 3678 Fragments arranged by size: 3678 3013 804 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3161 5489 6699 3161 Size: 2328 1210 4059 Fragments arranged by size: 4059 2328 1210 BclI T'GATC_A Cuts at: 1548 2475 1548 Size: 927 6670 BfrBI ATG'CAT Cuts at: 2374 2374 Size: 7597 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6208 136 Size: 107 122 71 631 605 4536 1525 Fragments arranged by size: 4536 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3553 4861 7592 3553 Size: 1308 2731 3558 Fragments arranged by size: 3558 2731 1308 BlpI GC'TnA_GC Cuts at: 5014 5014 Size: 7597 Bme1580I G_kGCm'C Cuts at: 1790 2359 4011 5658 6814 1790 Size: 569 1652 1647 1156 2573 Fragments arranged by size: 2573 1652 1647 1156 569 BmgBI CAC'GTC Cuts at: 1318 2422 1318 Size: 1104 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5328 6286 442 Size: 416 53 83 399 24 3911 958 1753 Fragments arranged by size: 3911 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7597 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 2947 3011 3428 3696 4143 4365 4668 Size: 923 785 64 417 268 447 222 303 Cuts at: 4668 6257 1239 Size: 1589 2579 Fragments arranged by size: 2579 1589 923 785 447 417 303 268 222 64 Bpu10I CC'TnA_GC Cuts at: 2034 2034 Size: 7597 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5722 6587 6885 5722 Size: 865 298 6434 Fragments arranged by size: 6434 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6260 882 Size: 617 4761 2219 Fragments arranged by size: 4761 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5388 493 Size: 711 4184 2702 Fragments arranged by size: 4184 2702 711 BsaBI GATnn'nnATC Cuts at: 3378 3531 3552 3558 3564 7591 3378 Size: 153 21 6 6 4027 3384 Fragments arranged by size: 4027 3384 153 21 6 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2461 5404 5786 6421 Size: 53 83 186 936 928 2943 382 635 Cuts at: 6421 275 Size: 1451 Fragments arranged by size: 2943 1451 936 928 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3885 6030 6428 6702 6849 7260 3885 Size: 2145 398 274 147 411 4222 Fragments arranged by size: 4222 2145 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2672 2702 1178 Size: 30 165 30 1269 30 6073 Fragments arranged by size: 6073 1269 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3521 4757 1186 Size: 2335 1236 4026 Fragments arranged by size: 4026 2335 1236 BseYI C'CCAG_C Cuts at: 3120 6800 7296 3120 Size: 3680 496 3421 Fragments arranged by size: 3680 3421 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3261 1163 Size: 573 1525 5499 Fragments arranged by size: 5499 1525 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5047 5808 6836 1540 Size: 275 186 3046 761 1028 2301 Fragments arranged by size: 3046 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2152 2173 2359 2685 2729 2997 5658 5743 6814 Size: 21 186 326 44 268 2661 85 1071 Cuts at: 6814 2152 Size: 2935 Fragments arranged by size: 2935 2661 1071 326 268 186 85 44 21 BsmI GAATG_Cn' Cuts at: 2376 2560 3122 5108 5201 2376 Size: 184 562 1986 93 4772 Fragments arranged by size: 4772 1986 562 184 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2468 2468 Size: 7597 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2173 2359 2685 2729 2997 4011 Size: 399 362 21 186 326 44 268 1014 Cuts at: 4011 4916 5658 5743 6814 1391 Size: 905 742 85 1071 2174 Fragments arranged by size: 2174 1071 1014 905 742 399 362 326 268 186 85 44 21 BspEI T'CCGG_A Cuts at: 7260 7260 Size: 7597 BspHI T'CATG_A Cuts at: 4794 5486 7216 4794 Size: 692 1730 5175 Fragments arranged by size: 5175 1730 692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3746 3937 4301 4952 844 Size: 2902 191 364 651 3489 Fragments arranged by size: 3489 2902 651 364 191 BsrBI CCG'CTC Cuts at: 1394 1823 5484 1394 Size: 429 3661 3507 Fragments arranged by size: 3661 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2028 2092 2161 4002 6092 6266 65 Size: 1183 780 64 69 1841 2090 174 1396 Fragments arranged by size: 2090 1841 1396 1183 780 174 69 64 BsrFI r'CCGG_y Cuts at: 1700 1810 4485 6241 6428 1700 Size: 110 2675 1756 187 2869 Fragments arranged by size: 2869 2675 1756 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7597 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7597 BssSI C'ACGA_G Cuts at: 3414 3536 5657 6669 3414 Size: 122 2121 1012 4342 Fragments arranged by size: 4342 2121 1012 122 BstAPI GCAn_nnn'nTGC Cuts at: 794 2689 3743 794 Size: 1895 1054 4648 Fragments arranged by size: 4648 1895 1054 BstBI TT'CG_AA Cuts at: 4327 6401 4327 Size: 2074 5523 BstEII G'GTnAC_C Cuts at: 1184 4570 1184 Size: 3386 4211 BstYI r'GATC_y Cuts at: 1972 2733 2884 3553 3559 3565 4861 4927 5294 Size: 761 151 669 6 6 1296 66 367 Cuts at: 5294 5679 5696 7148 7234 7246 7592 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1296 761 669 385 367 346 151 86 66 17 12 6 6 BtgI C'CryG_G Cuts at: 513 1065 2004 2769 3766 513 Size: 552 939 765 997 4344 Fragments arranged by size: 4344 997 939 765 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6858 Fragments arranged by size: 6858 683 56 BtsI GCAGTG_nn' Cuts at: 2565 4117 4341 4496 5113 5907 5927 2565 Size: 1552 224 155 617 794 20 4235 Fragments arranged by size: 4235 1552 794 617 224 155 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7597 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7562 DraI TTT'AAA Cuts at: 2392 4020 4901 2392 Size: 1628 881 5088 Fragments arranged by size: 5088 1628 881 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6604 809 Size: 507 5288 1802 Fragments arranged by size: 5288 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4050 5044 5933 Size: 54 1425 50 275 18 2220 994 889 Cuts at: 5933 7562 8 Size: 1629 43 Fragments arranged by size: 2220 1629 1425 994 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5044 1537 Size: 275 3232 4090 Fragments arranged by size: 4090 3232 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2244 2433 2910 3708 4268 4324 4746 5527 Size: 891 189 477 798 560 56 422 781 Cuts at: 5527 1353 Size: 3423 Fragments arranged by size: 3423 891 798 781 560 477 422 189 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2394 6184 6558 6704 387 Size: 1064 138 805 3790 374 146 1280 Fragments arranged by size: 3790 1280 1064 805 374 146 138 EcoICRI GAG'CTC Cuts at: 2150 2727 2995 2150 Size: 577 268 6752 Fragments arranged by size: 6752 577 268 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2615 2654 2947 3011 3428 3696 Size: 393 530 453 39 293 64 417 268 Cuts at: 3696 4143 4365 4407 4500 4665 4668 4692 4713 Size: 447 222 42 93 165 3 24 21 Cuts at: 4713 4878 5660 6257 7044 7312 1239 Size: 165 782 597 787 268 1524 Fragments arranged by size: 1524 787 782 597 530 453 447 417 393 293 268 268 222 165 165 93 64 42 39 24 21 3 EcoNI CCTnn'n_nnAGG Cuts at: 4695 4695 Size: 7597 EcoO109I rG'GnC_Cy Cuts at: 3749 4853 3749 Size: 1104 6493 EcoRI G'AATT_C Cuts at: 4906 5284 4906 Size: 378 7219 EcoRV GAT'ATC Cuts at: 2718 2718 Size: 7597 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3338 3338 Size: 7597 FspI TGC'GCA Cuts at: 6103 6103 Size: 7597 HaeII r_GCGC'y Cuts at: 1678 2131 6744 1678 Size: 453 4613 2531 Fragments arranged by size: 4613 2531 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2702 2734 2869 2901 6238 Size: 32 135 32 1130 32 135 32 3337 Cuts at: 6238 6270 6312 6344 1373 Size: 32 42 32 2626 Fragments arranged by size: 3337 2626 1130 135 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4941 5187 669 Size: 988 281 3003 246 3079 Fragments arranged by size: 3079 3003 988 281 246 HindIII A'AGCT_T Cuts at: 748 2603 4497 748 Size: 1855 1894 3848 Fragments arranged by size: 3848 1894 1855 HpaI GTT'AAC Cuts at: 5187 5187 Size: 7597 KpnI G_GTAC'C Cuts at: 4922 4922 Size: 7597 MfeI C'AATT_G Cuts at: 4243 5196 4243 Size: 953 6644 MluI A'CGCG_T Cuts at: 7473 7473 Size: 7597 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3091 3249 3319 3852 6711 6895 1305 Size: 1786 158 70 533 2859 184 2007 Fragments arranged by size: 2859 2007 1786 533 184 158 70 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5775 Fragments arranged by size: 5775 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2083 2166 2352 2429 3771 3811 Size: 648 891 26 83 186 77 1342 40 Cuts at: 3811 4761 4793 5555 5914 6073 518 Size: 950 32 762 359 159 2042 Fragments arranged by size: 2042 1342 950 891 762 648 359 186 159 83 77 40 32 26 MspA1I CmG'CkG Cuts at: 1606 2118 2593 3002 3740 3821 4685 5013 5690 Size: 512 475 409 738 81 864 328 677 Cuts at: 5690 6838 7083 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 864 738 677 512 475 409 328 245 81 NcoI C'CATG_G Cuts at: 513 1065 2004 2769 3766 513 Size: 552 939 765 997 4344 Fragments arranged by size: 4344 997 939 765 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7597 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7597 NotI GC'GGCC_GC Cuts at: 5044 5044 Size: 7597 NsiI A_TGCA'T Cuts at: 2376 2376 Size: 7597 NspI r_CATG'y Cuts at: 1132 1229 3435 3445 4132 4176 4955 6500 1132 Size: 97 2206 10 687 44 779 1545 2229 Fragments arranged by size: 2229 2206 1545 779 687 97 44 10 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7597 PciI A'CATG_T Cuts at: 6496 6496 Size: 7597 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7597 PmeI GTTT'AAAC Cuts at: 4901 4901 Size: 7597 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5666 5698 7179 7211 5666 Size: 32 1481 32 6052 Fragments arranged by size: 6052 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3749 3749 Size: 7597 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7597 PsiI TTA'TAA Cuts at: 2609 5167 2609 Size: 2558 5039 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3030 3062 3030 Size: 32 7565 PstI C_TGCA'G Cuts at: 830 2252 3748 3945 4617 4949 830 Size: 1422 1496 197 672 332 3478 Fragments arranged by size: 3478 1496 1422 672 332 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7597 PvuII CAG'CTG Cuts at: 2118 2593 3002 3740 4685 2118 Size: 475 409 738 945 5030 Fragments arranged by size: 5030 945 738 475 409 SacI G_AGCT'C Cuts at: 2152 2729 2997 2152 Size: 577 268 6752 Fragments arranged by size: 6752 577 268 SalI G'TCGA_C Cuts at: 1936 4939 1936 Size: 3003 4594 SapI GCTCTTCn'nnn_ Cuts at: 4324 4746 4324 Size: 422 7175 SbfI CC_TGCA'GG Cuts at: 4949 4949 Size: 7597 ScaI AGT'ACT Cuts at: 5845 7557 5845 Size: 1712 5885 SfcI C'TryA_G Cuts at: 826 1045 2248 3465 3744 3941 4613 4945 6080 Size: 219 1203 1217 279 197 672 332 1135 Cuts at: 6080 6761 6952 826 Size: 681 191 1471 Fragments arranged by size: 1471 1217 1203 1135 681 672 332 279 219 197 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7597 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7597 SmaI CCC'GGG Cuts at: 1872 3242 4924 1872 Size: 1370 1682 4545 Fragments arranged by size: 4545 1682 1370 SmlI C'TyrA_G Cuts at: 820 1017 1943 3878 5701 6602 6864 820 Size: 197 926 1935 1823 901 262 1553 Fragments arranged by size: 1935 1823 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5388 493 Size: 4895 2702 SpeI A'CTAG_T Cuts at: 152 2191 3284 152 Size: 2039 1093 4465 Fragments arranged by size: 4465 2039 1093 SphI G_CATG'C Cuts at: 1132 3435 4955 1132 Size: 2303 1520 3774 Fragments arranged by size: 3774 2303 1520 SspI AAT'ATT Cuts at: 5 52 5521 5 Size: 47 5469 2081 Fragments arranged by size: 5469 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7597 StyI C'CwwG_G Cuts at: 513 1065 2004 2769 3766 4575 5036 513 Size: 552 939 765 997 809 461 3074 Fragments arranged by size: 3074 997 939 809 765 552 461 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5624 5641 5794 5979 6424 325 Size: 1255 4044 17 153 185 445 1498 Fragments arranged by size: 4044 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4663 5843 7555 Size: 274 80 33 51 1443 2686 1180 1712 Cuts at: 7555 96 Size: 138 Fragments arranged by size: 2686 1712 1443 1180 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2779 2811 7557 7589 2779 Size: 32 4746 32 2787 Fragments arranged by size: 4746 2787 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4381 1316 Size: 3065 4532 XbaI T'CTAG_A Cuts at: 4933 4933 Size: 7597 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7597 XmaI C'CCGG_G Cuts at: 1870 3240 4922 1870 Size: 1370 1682 4545 Fragments arranged by size: 4545 1682 1370 XmnI GAAnn'nnTTC Cuts at: 5726 5726 Size: 7597 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5405 276 Size: 53 83 186 936 3871 2468 Fragments arranged by size: 3871 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI