(Circular) (Six-base) MAPSORT of: pFN21AE0127.seq Check: 4734 from: 1 to: 5833 pFN21AE0127 5833 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 2010 3154 2010 Size: 1144 4689 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2027 2075 2555 2661 3896 5280 5548 1632 Size: 395 48 480 106 1235 1384 268 1917 Fragments arranged by size: 1917 1384 1235 480 395 268 106 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 2331 3084 4562 2331 Size: 753 1478 3602 Fragments arranged by size: 3602 1478 753 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2828 1184 Size: 1644 4189 AlwNI CAG_nnn'CTG Cuts at: 3576 5148 3576 Size: 1572 4261 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 2867 3142 3368 3520 5629 1493 Size: 1374 275 226 152 2109 1697 Fragments arranged by size: 2109 1697 1374 275 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2010 2127 2857 3154 4609 Size: 325 217 627 223 117 730 297 1455 Cuts at: 4609 618 Size: 1842 Fragments arranged by size: 1842 1455 730 627 325 297 223 217 117 BanII G_rGCy'C Cuts at: 1391 2757 3152 1391 Size: 1366 395 4072 Fragments arranged by size: 4072 1366 395 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4862 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2223 2257 4024 4058 1903 Size: 34 286 34 1767 34 3678 Fragments arranged by size: 3678 1767 286 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2284 3725 4935 2284 Size: 1441 1210 3182 Fragments arranged by size: 3182 1441 1210 BclI T'GATC_A Cuts at: 1548 2994 1548 Size: 1446 4387 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 2248 3250 2248 Size: 1002 4831 Bme1580I G_kGCm'C Cuts at: 1790 3894 5050 1790 Size: 2104 1156 2573 Fragments arranged by size: 2573 2104 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2717 1056 Size: 1661 4172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2671 4493 1239 Size: 1432 1822 2579 Fragments arranged by size: 2579 1822 1432 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2583 3958 4823 5121 2583 Size: 1375 865 298 3295 Fragments arranged by size: 3295 1375 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2229 2336 4496 882 Size: 617 730 107 2160 2219 Fragments arranged by size: 2219 2160 730 617 107 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5608 Fragments arranged by size: 5608 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2076 2867 3034 1186 Size: 890 791 167 3985 Fragments arranged by size: 3985 890 791 167 BseYI C'CCAG_C Cuts at: 5036 5532 5036 Size: 496 5337 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5260 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2607 3894 3979 5050 2607 Size: 1287 85 1071 3390 Fragments arranged by size: 3390 1287 1071 85 BsmI GAATG_Cn' Cuts at: 2467 2573 2763 3344 3437 2467 Size: 106 190 581 93 4863 Fragments arranged by size: 4863 581 190 106 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3100 3100 Size: 5833 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2607 2757 3152 3894 3979 5050 1391 Size: 399 817 150 395 742 85 1071 2174 Fragments arranged by size: 2174 1071 817 742 399 395 150 85 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3188 844 Size: 2344 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2247 3720 1394 Size: 429 424 1473 3507 Fragments arranged by size: 3507 1473 429 424 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4477 4664 1700 Size: 110 2667 187 2869 Fragments arranged by size: 2869 2667 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5833 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstXI CCAn_nnnn'nTGG Cuts at: 2335 2942 2335 Size: 607 5226 BstYI r'GATC_y Cuts at: 1972 3163 3530 3915 3932 5384 5470 5482 5828 Size: 1191 367 385 17 1452 86 12 346 Cuts at: 5828 1972 Size: 1977 Fragments arranged by size: 1977 1452 1191 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2862 2862 Size: 5833 BtgI C'CryG_G Cuts at: 513 1065 2972 513 Size: 552 1907 3374 Fragments arranged by size: 3374 1907 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 2239 2697 2810 3041 3349 4143 4163 2239 Size: 458 113 231 308 794 20 3909 Fragments arranged by size: 3909 794 458 308 231 113 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2363 3280 4169 Size: 54 1425 50 275 18 533 917 889 Cuts at: 4169 5798 8 Size: 1629 43 Fragments arranged by size: 1629 1425 917 889 533 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3763 1353 Size: 2410 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2027 2075 2555 2661 2671 3896 4493 Size: 393 395 48 480 106 10 1225 597 Cuts at: 4493 5280 5548 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1225 787 597 480 395 393 268 106 48 10 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 4980 1678 Size: 3302 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2317 2349 3070 3102 4474 Size: 32 135 32 745 32 721 32 1372 Cuts at: 4474 4506 4548 4580 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1372 745 721 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3028 3177 3423 669 Size: 988 281 1090 149 246 3079 Fragments arranged by size: 3079 1090 988 281 246 149 HindIII A'AGCT_T Cuts at: 748 2101 748 Size: 1353 4480 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 2014 3158 2014 Size: 1144 4689 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2899 4947 5131 1305 Size: 1594 2048 184 2007 Fragments arranged by size: 2048 2007 1594 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2365 10 Size: 54 1425 343 533 3478 Fragments arranged by size: 3478 1425 533 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2768 3052 3791 4150 4309 518 Size: 648 1602 284 739 359 159 2042 Fragments arranged by size: 2042 1602 739 648 359 284 159 MspA1I CmG'CkG Cuts at: 1606 3249 3926 5074 5319 1606 Size: 1643 677 1148 245 2120 Fragments arranged by size: 2120 1643 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2972 513 Size: 552 1907 3374 Fragments arranged by size: 3374 1907 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 2713 1052 Size: 1661 4172 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 1132 Size: 97 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 3185 830 Size: 2355 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 3181 4316 4997 5188 826 Size: 219 2136 1135 681 191 1471 Fragments arranged by size: 2136 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2598 3937 4838 5100 820 Size: 197 926 655 1339 901 262 1553 Fragments arranged by size: 1553 1339 926 901 655 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3075 3757 5 Size: 47 3023 682 2081 Fragments arranged by size: 3023 2081 682 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2972 3272 513 Size: 552 1907 300 3074 Fragments arranged by size: 3074 1907 552 300 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3860 3877 4030 4215 4660 325 Size: 1255 2280 17 153 185 445 1498 Fragments arranged by size: 2280 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4079 5791 96 Size: 274 80 33 51 1443 2102 1712 138 Fragments arranged by size: 2102 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2006 2038 5793 5825 2006 Size: 32 3755 32 2014 Fragments arranged by size: 3755 2014 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2716 2716 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI