(Circular) (Six-base) MAPSORT of: pFN21AE0008.seq Check: 3107 from: 1 to: 6796 pFN21AE0008 6796 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3539 3539 Size: 6796 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4606 278 Size: 53 83 186 936 3070 2468 Fragments arranged by size: 3070 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 4139 1937 Size: 1398 804 4594 Fragments arranged by size: 4594 1398 804 Acc65I G'GTAC_C Cuts at: 4117 4117 Size: 6796 AclI AA'CG_TT Cuts at: 1563 4923 5296 1563 Size: 3360 373 3063 Fragments arranged by size: 3360 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2082 4859 6243 6511 1632 Size: 450 2777 1384 268 1917 Fragments arranged by size: 2777 1917 1384 450 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6599 AflIII A'CryG_T Cuts at: 1203 3310 5695 6672 1203 Size: 2107 2385 977 1327 Fragments arranged by size: 2385 2107 1327 977 AgeI A'CCGG_T Cuts at: 5627 5627 Size: 6796 AhdI GACnn_n'nnGTC Cuts at: 2801 5525 2801 Size: 2724 4072 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6796 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6796 AlwNI CAG_nnn'CTG Cuts at: 2750 4539 6111 2750 Size: 1789 1572 3435 Fragments arranged by size: 3435 1789 1572 ApaLI G'TGCA_C Cuts at: 4853 6009 4853 Size: 1156 5640 ApoI r'AATT_y Cuts at: 1493 2185 2430 2477 2784 2910 3144 3325 3646 Size: 692 245 47 307 126 234 181 321 Cuts at: 3646 4105 4331 4483 6592 1493 Size: 459 226 152 2109 1697 Fragments arranged by size: 2109 1697 692 459 321 307 245 234 226 181 152 126 47 AseI AT'TA_AT Cuts at: 160 1032 3681 5350 160 Size: 872 2649 1669 1606 Fragments arranged by size: 2649 1669 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6796 AvaI C'yCGr_G Cuts at: 1870 1943 4121 1870 Size: 73 2178 4545 Fragments arranged by size: 4545 2178 73 BamHI G'GATC_C Cuts at: 4126 4493 4126 Size: 367 6429 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4117 5572 618 Size: 325 217 627 2330 1455 1842 Fragments arranged by size: 2330 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3201 3544 4115 1391 Size: 1810 343 571 4072 Fragments arranged by size: 4072 1810 571 343 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2800 3086 928 Size: 971 901 286 4638 Fragments arranged by size: 4638 971 901 286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4987 5021 1903 Size: 34 3050 34 3678 Fragments arranged by size: 3678 3050 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4688 5898 4688 Size: 1210 5586 BclI T'GATC_A Cuts at: 1548 1548 Size: 6796 BfrBI ATG'CAT Cuts at: 2092 2092 Size: 6796 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5407 136 Size: 107 122 71 631 605 3735 1525 Fragments arranged by size: 3735 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6791 6791 Size: 6796 BlpI GC'TnA_GC Cuts at: 4213 4213 Size: 6796 Bme1580I G_kGCm'C Cuts at: 1790 3519 4857 6013 1790 Size: 1729 1338 1156 2573 Fragments arranged by size: 2573 1729 1338 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6796 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3787 4527 5485 Size: 416 53 83 399 24 2370 740 958 Cuts at: 5485 442 Size: 1753 Fragments arranged by size: 2370 1753 958 740 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6796 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 5456 1239 Size: 1265 2952 2579 Fragments arranged by size: 2952 2579 1265 Bpu10I CC'TnA_GC Cuts at: 2152 2152 Size: 6796 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3172 4921 5786 6084 3172 Size: 1749 865 298 3884 Fragments arranged by size: 3884 1749 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3380 5459 882 Size: 617 1881 2079 2219 Fragments arranged by size: 2219 2079 1881 617 BsaAI yAC'GTr Cuts at: 493 1204 2164 3495 4587 493 Size: 711 960 1331 1092 2702 Fragments arranged by size: 2702 1331 1092 960 711 BsaBI GATnn'nnATC Cuts at: 6790 6790 Size: 6796 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4603 4985 5620 275 Size: 53 83 186 936 3070 382 635 1451 Fragments arranged by size: 3070 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5229 5627 5901 6048 6459 5229 Size: 398 274 147 411 5566 Fragments arranged by size: 5566 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3782 3812 1178 Size: 30 165 30 2379 30 4162 Fragments arranged by size: 4162 2379 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6796 BseYI C'CCAG_C Cuts at: 5999 6495 5999 Size: 496 6300 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6223 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4246 5007 6035 1540 Size: 275 186 2245 761 1028 2301 Fragments arranged by size: 2301 2245 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2417 4857 4942 6013 2417 Size: 2440 85 1071 3200 Fragments arranged by size: 3200 2440 1071 85 BsmI GAATG_Cn' Cuts at: 2092 2849 3436 4307 4400 2092 Size: 757 587 871 93 4488 Fragments arranged by size: 4488 871 757 587 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2417 3201 3519 3544 4115 4857 4942 Size: 399 627 784 318 25 571 742 85 Cuts at: 4942 6013 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 784 742 627 571 399 318 85 25 BspEI T'CCGG_A Cuts at: 6459 6459 Size: 6796 BspHI T'CATG_A Cuts at: 4685 6415 4685 Size: 1730 5066 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3539 4151 844 Size: 2695 612 3489 Fragments arranged by size: 3489 2695 612 BsrBI CCG'CTC Cuts at: 1394 1823 4683 1394 Size: 429 2860 3507 Fragments arranged by size: 3507 2860 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2058 2502 5291 5465 65 Size: 1183 810 444 2789 174 1396 Fragments arranged by size: 2789 1396 1183 810 444 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5440 5627 1700 Size: 110 3630 187 2869 Fragments arranged by size: 3630 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3313 96 Size: 3217 3579 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6796 BssSI C'ACGA_G Cuts at: 4856 5868 4856 Size: 1012 5784 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6796 BstBI TT'CG_AA Cuts at: 5600 5600 Size: 6796 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6796 BstYI r'GATC_y Cuts at: 1972 3996 4126 4493 4878 4895 6347 6433 6445 Size: 2024 130 367 385 17 1452 86 12 Cuts at: 6445 6791 1972 Size: 346 1977 Fragments arranged by size: 2024 1977 1452 385 367 346 130 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2776 3186 3523 513 Size: 552 1711 410 337 3786 Fragments arranged by size: 3786 1711 552 410 337 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3918 487 Size: 683 56 2692 3365 Fragments arranged by size: 3365 2692 683 56 BtsI GCAGTG_nn' Cuts at: 3293 3705 4312 5106 5126 3293 Size: 412 607 794 20 4963 Fragments arranged by size: 4963 794 607 412 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6796 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6761 DraI TTT'AAA Cuts at: 2347 4100 2347 Size: 1753 5043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5803 809 Size: 507 4487 1802 Fragments arranged by size: 4487 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3909 4243 5132 Size: 54 1425 50 275 18 2079 334 889 Cuts at: 5132 6761 8 Size: 1629 43 Fragments arranged by size: 2079 1629 1425 889 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4243 1537 Size: 275 2431 4090 Fragments arranged by size: 4090 2431 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3206 4726 1353 Size: 1853 1520 3423 Fragments arranged by size: 3423 1853 1520 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5383 5757 5903 387 Size: 1064 138 3794 374 146 1280 Fragments arranged by size: 3794 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2082 2504 4859 5456 6243 6511 1239 Size: 393 450 422 2355 597 787 268 1524 Fragments arranged by size: 2355 1524 787 597 450 422 393 268 EcoRI G'AATT_C Cuts at: 4105 4483 4105 Size: 378 6418 FspI TGC'GCA Cuts at: 5302 5302 Size: 6796 HaeII r_GCGC'y Cuts at: 1678 5943 1678 Size: 4265 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 2566 2598 2695 Size: 32 135 32 752 32 210 32 97 Cuts at: 2695 2727 2787 2819 2911 2943 5437 5469 5511 Size: 32 60 32 92 32 2494 32 42 Cuts at: 5511 5543 1373 Size: 32 2626 Fragments arranged by size: 2626 2494 752 210 135 97 92 60 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4140 4386 669 Size: 988 281 2202 246 3079 Fragments arranged by size: 3079 2202 988 281 246 HindIII A'AGCT_T Cuts at: 748 3450 748 Size: 2702 4094 HpaI GTT'AAC Cuts at: 4386 4386 Size: 6796 KpnI G_GTAC'C Cuts at: 4121 4121 Size: 6796 MfeI C'AATT_G Cuts at: 4395 4395 Size: 6796 MluI A'CGCG_T Cuts at: 6672 6672 Size: 6796 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2473 3220 5910 6094 1305 Size: 1168 747 2690 184 2007 Fragments arranged by size: 2690 2007 1168 747 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3911 10 Size: 54 1425 343 2079 2895 Fragments arranged by size: 2895 2079 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2516 3522 4754 5113 5272 518 Size: 648 1350 1006 1232 359 159 2042 Fragments arranged by size: 2042 1350 1232 1006 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4212 4889 6037 6282 1606 Size: 2606 677 1148 245 2120 Fragments arranged by size: 2606 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3186 3523 513 Size: 552 2121 337 3786 Fragments arranged by size: 3786 2121 552 337 NdeI CA'TA_TG Cuts at: 387 3378 387 Size: 2991 3805 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6796 NotI GC'GGCC_GC Cuts at: 4243 4243 Size: 6796 NsiI A_TGCA'T Cuts at: 2094 2094 Size: 6796 NspI r_CATG'y Cuts at: 1132 1229 2092 3160 3314 3346 4154 5699 1132 Size: 97 863 1068 154 32 808 1545 2229 Fragments arranged by size: 2229 1545 1068 863 808 154 97 32 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6796 PciI A'CATG_T Cuts at: 3310 5695 3310 Size: 2385 4411 PflMI CCAn_nnn'nTGG Cuts at: 1108 2295 3140 3211 3918 1108 Size: 1187 845 71 707 3986 Fragments arranged by size: 3986 1187 845 707 71 PmeI GTTT'AAAC Cuts at: 4100 4100 Size: 6796 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4865 4897 6378 6410 4865 Size: 32 1481 32 5251 Fragments arranged by size: 5251 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6796 PsiI TTA'TAA Cuts at: 4366 4366 Size: 6796 PstI C_TGCA'G Cuts at: 830 3275 4148 830 Size: 2445 873 3478 Fragments arranged by size: 3478 2445 873 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6796 SalI G'TCGA_C Cuts at: 1936 4138 1936 Size: 2202 4594 SapI GCTCTTCn'nnn_ Cuts at: 3206 3206 Size: 6796 SbfI CC_TGCA'GG Cuts at: 4148 4148 Size: 6796 ScaI AGT'ACT Cuts at: 5044 6756 5044 Size: 1712 5084 SfcI C'TryA_G Cuts at: 826 1045 3271 4144 5279 5960 6151 826 Size: 219 2226 873 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 873 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6796 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6796 SmaI CCC'GGG Cuts at: 1872 4123 1872 Size: 2251 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3151 4900 5801 6063 820 Size: 197 926 1208 1749 901 262 1553 Fragments arranged by size: 1749 1553 1208 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2164 3495 4587 493 Size: 1671 1331 1092 2702 Fragments arranged by size: 2702 1671 1331 1092 SpeI A'CTAG_T Cuts at: 152 152 Size: 6796 SphI G_CATG'C Cuts at: 1132 3346 4154 1132 Size: 2214 808 3774 Fragments arranged by size: 3774 2214 808 SspI AAT'ATT Cuts at: 5 52 2378 4720 5 Size: 47 2326 2342 2081 Fragments arranged by size: 2342 2326 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6796 StyI C'CwwG_G Cuts at: 513 1065 3186 3523 3656 4235 513 Size: 552 2121 337 133 579 3074 Fragments arranged by size: 3074 2121 579 552 337 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 3649 4823 4840 4993 5178 5623 Size: 1255 1956 113 1174 17 153 185 445 Cuts at: 5623 325 Size: 1498 Fragments arranged by size: 1956 1498 1255 1174 445 185 153 113 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3313 5042 6754 Size: 274 80 33 51 1443 1336 1729 1712 Cuts at: 6754 96 Size: 138 Fragments arranged by size: 1729 1712 1443 1336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3822 3854 3891 3923 6756 6788 3822 Size: 32 37 32 2833 32 3830 Fragments arranged by size: 3830 2833 37 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6796 XbaI T'CTAG_A Cuts at: 4132 4132 Size: 6796 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6796 XmaI C'CCGG_G Cuts at: 1870 4121 1870 Size: 2251 4545 XmnI GAAnn'nnTTC Cuts at: 2597 2797 4925 2597 Size: 200 2128 4468 Fragments arranged by size: 4468 2128 200 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4604 276 Size: 53 83 186 936 3070 2468 Fragments arranged by size: 3070 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI