Restriction map for
pFN21AB7651
(Linear) (Six-base) MAPSORT of: KIBB7651.seq Check: 3028 from: 1 to: 990
KIBB7651.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AfeI AGC'GCT
Cuts at: 0 381 655 990
Size: 381 274 335
Fragments arranged by size:
381 335 274
AleI CACnn'nnGTG
Cuts at: 0 970 990
Size: 970 20
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 841 990
Size: 841 149
AlwNI CAG_nnn'CTG
Cuts at: 0 447 870 924 990
Size: 447 423 54 66
Fragments arranged by size:
447 423 66 54
ApaLI G'TGCA_C
Cuts at: 0 892 990
Size: 892 98
AvaI C'yCGr_G
Cuts at: 0 30 990
Size: 30 960
BanI G'GyrC_C
Cuts at: 0 157 268 364 399 990
Size: 157 111 96 35 591
Fragments arranged by size:
591 157 111 96 35
BanII G_rGCy'C
Cuts at: 0 47 184 990
Size: 47 137 806
Fragments arranged by size:
806 137 47
BbeI G_GCGC'C
Cuts at: 0 161 272 990
Size: 161 111 718
Fragments arranged by size:
718 161 111
BglI GCCn_nnn'nGGC
Cuts at: 0 248 257 700 990
Size: 248 9 443 290
Fragments arranged by size:
443 290 248 9
BglII A'GATC_T
Cuts at: 0 635 990
Size: 635 355
Bme1580I G_kGCm'C
Cuts at: 0 375 473 896 990
Size: 375 98 423 94
Fragments arranged by size:
423 375 98 94
BmgBI CAC'GTC
Cuts at: 0 617 968 990
Size: 617 351 22
Fragments arranged by size:
617 351 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 224 248 667 990
Size: 103 121 24 419 323
Fragments arranged by size:
419 323 121 103 24
BsaHI Gr'CG_yC
Cuts at: 0 158 269 494 589 976 990
Size: 158 111 225 95 387 14
Fragments arranged by size:
387 225 158 111 95 14
BsaWI w'CCGG_w
Cuts at: 0 584 990
Size: 584 406
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 221 990
Size: 221 769
BseYI C'CCAG_C
Cuts at: 0 440 990
Size: 440 550
BsiEI CG_ry'CG
Cuts at: 0 191 686 990
Size: 191 495 304
Fragments arranged by size:
495 304 191
BsiHKAI G_wGCw'C
Cuts at: 0 8 896 990
Size: 8 888 94
Fragments arranged by size:
888 94 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 762 990
Size: 762 228
Bsp1286I G_dGCh'C
Cuts at: 0 8 47 184 375 473 896 990
Size: 8 39 137 191 98 423 94
Fragments arranged by size:
423 191 137 98 94 39 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 720 990
Size: 720 270
BsrBI CCG'CTC
Cuts at: 0 211 417 990
Size: 211 206 573
Fragments arranged by size:
573 211 206
BsrFI r'CCGG_y
Cuts at: 0 292 402 454 752 990
Size: 292 110 52 298 238
Fragments arranged by size:
298 292 238 110 52
BssHII G'CGCG_C
Cuts at: 0 690 990
Size: 690 300
BstAPI GCAn_nnn'nTGC
Cuts at: 0 924 990
Size: 924 66
BstEII G'GTnAC_C
Cuts at: 0 568 990
Size: 568 422
BstYI r'GATC_y
Cuts at: 0 68 635 990
Size: 68 567 355
Fragments arranged by size:
567 355 68
BtgI C'CryG_G
Cuts at: 0 185 428 931 990
Size: 185 243 503 59
Fragments arranged by size:
503 243 185 59
EaeI y'GGCC_r
Cuts at: 0 188 449 683 701 990
Size: 188 261 234 18 289
Fragments arranged by size:
289 261 234 188 18
EagI C'GGCC_G
Cuts at: 0 188 683 990
Size: 188 495 307
Fragments arranged by size:
495 307 188
EarI CTCTTCn'nnn_
Cuts at: 0 847 913 990
Size: 847 66 77
Fragments arranged by size:
847 77 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 771 990
Size: 771 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 224 248 667 990
Size: 103 121 24 419 323
Fragments arranged by size:
419 323 121 103 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 990
Size: 703 287
EcoO109I rG'GnC_Cy
Cuts at: 0 103 215 232 283 990
Size: 103 112 17 51 707
Fragments arranged by size:
707 112 103 51 17
FspI TGC'GCA
Cuts at: 0 728 990
Size: 728 262
HaeII r_GCGC'y
Cuts at: 0 161 272 383 657 712 990
Size: 161 111 111 274 55 278
Fragments arranged by size:
278 274 161 111 111 55
HincII GTy'rAC
Cuts at: 0 498 990
Size: 498 492
KasI G'GCGC_C
Cuts at: 0 157 268 990
Size: 157 111 722
Fragments arranged by size:
722 157 111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 990
Size: 132 858
MscI TGG'CCA
Cuts at: 0 451 703 990
Size: 451 252 287
Fragments arranged by size:
451 287 252
MslI CAynn'nnrTG
Cuts at: 0 970 990
Size: 970 20
MspA1I CmG'CkG
Cuts at: 0 187 359 870 877 990
Size: 187 172 511 7 113
Fragments arranged by size:
511 187 172 113 7
NaeI GCC'GGC
Cuts at: 0 404 990
Size: 404 586
NarI GG'CG_CC
Cuts at: 0 158 269 990
Size: 158 111 721
Fragments arranged by size:
721 158 111
NgoMIV G'CCGG_C
Cuts at: 0 402 990
Size: 402 588
NotI GC'GGCC_GC
Cuts at: 0 188 990
Size: 188 802
PflMI CCAn_nnn'nTGG
Cuts at: 0 447 990
Size: 447 543
PfoI T'CCnGG_A
Cuts at: 0 119 990
Size: 119 871
PpuMI rG'GwC_Cy
Cuts at: 0 103 232 283 990
Size: 103 129 51 707
Fragments arranged by size:
707 129 103 51
PstI C_TGCA'G
Cuts at: 0 277 635 830 920 929 990
Size: 277 358 195 90 9 61
Fragments arranged by size:
358 277 195 90 61 9
SacII CC_GC'GG
Cuts at: 0 188 990
Size: 188 802
SanDI GG'GwC_CC
Cuts at: 0 103 990
Size: 103 887
SapI GCTCTTCn'nnn_
Cuts at: 0 847 913 990
Size: 847 66 77
Fragments arranged by size:
847 77 66
SexAI A'CCwGG_T
Cuts at: 0 739 990
Size: 739 251
SfcI C'TryA_G
Cuts at: 0 273 631 826 916 925 990
Size: 273 358 195 90 9 65
Fragments arranged by size:
358 273 195 90 65 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 248 990
Size: 248 742
SfoI GGC'GCC
Cuts at: 0 159 270 990
Size: 159 111 720
Fragments arranged by size:
720 159 111
SmaI CCC'GGG
Cuts at: 0 32 990
Size: 32 958
StuI AGG'CCT
Cuts at: 0 844 990
Size: 844 146
StyI C'CwwG_G
Cuts at: 0 138 317 704 990
Size: 138 179 387 286
Fragments arranged by size:
387 286 179 138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 736 990
Size: 736 254
XmaI C'CCGG_G
Cuts at: 0 30 990
Size: 30 960
XmnI GAAnn'nnTTC
Cuts at: 0 655 990
Size: 655 335
Enzymes that do cut:
AfeI AleI AlfI AlwNI ApaLI AvaI BanI BanII
BbeI BglI BglII Bme1580I BmgBI BpmI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstYI BtgI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI PflMI PfoI PpuMI PstI SacII SanDI SapI
SexAI SfcI SfiI SfoI SmaI StuI StyI TaqII
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BsgI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI
EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SbfI ScaI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI
XhoI ZraI