Restriction map for
pFN21AB7319
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BlnI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI KspI MaeI MamI MluI
MroI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 747 GACGTC GACGT^C
Asp700I 1377 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 919 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 996 CCCGGG C^[CT]CG[GA]G
BbeI 883 GGCGCC GGCGC^C
BbsI 1669 GTCTTC GAAGAC(2/6)
BglII 1268 AGATCT A^GATCT
BlpI 55 GCTGAGC GC^T[ACGT]AGC
BpmI 1761 CTGGAG CTGGAG(16/14)
Bpu1102I 55 GCTGAGC GC^T[ACGT]AGC
BpuAI 1669 GTCTTC GAAGAC(2/6)
BsaAI 1861 TACGTG [CT]AC^GT[GA]
BsaWI 1367 ACCGGA [AT]^CCGG[AT]
BsmI 1600 GAATGC GAATGC(1/-1)
BsoBI 996 CCCGGG C^[CT]CG[GA]G
BspHI 440 TCATGA T^CATGA
BsrDI 1359 GCAATG GCAATG(2/0)
BssHII 805 GCGCGC G^CGCGC
BssSI 349 CACGAG CACGAG(-5/-1)
BstYI 1268 AGATCT [GA]^GATC[CT]
CelII 55 GCTGAGC GC^T[ACGT]AGC
DrdI 2374 GACCTCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 210 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1279 GTCAAC GT[CT]^[GA]AC
HindII 1279 GTCAAC GT[CT]^[GA]AC
KasI 883 GGCGCC G^GCGCC
MfeI 2147 CAATTG C^AATTG
MflI 1268 AGATCT [GA]^GATC[CT]
MseI 152 TTAA T^TAA
MunI 2147 CAATTG C^AATTG
NaeI 60 GCCGGC GCC^GGC
NarI 883 GGCGCC GG^CGCC
NcoI 578 CCATGG C^CATGG
NgoMIV 60 GCCGGC G^CCGGC
NspI 2087 GCATGC [GA]CATG^[CT]
PflFI 919 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 47 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 440 TCATGA T^CATGA
SbfI 159 CCTGCAGG CCTGCA^GG
SfiI 1707 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 883 GGCGCC GGC^GCC
SmaI 996 CCCGGG CCC^GGG
SmlI 1261 CTTGAG C^T[CT][GA]AG
SphI 2087 GCATGC GCATG^C
Sse8387I 159 CCTGCAGG CCTGCA^GG
StuI 1607 AGGCCT AGG^CCT
TfiI 1377 GAATC G^A[AT]TC
Tru9I 152 TTAA T^TAA
Tth111I 919 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1268 AGATCT [GA]^GATC[CT]
XmaCI 996 CCCGGG C^CCGGG
XmaI 996 CCCGGG C^CCGGG
XmnI 1377 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1153 GTCTAC GT^[AC][GT]AC
1243 GTCTAC
AcyI 747 GACGTC G[GA]^CG[CT]C
883 GGCGCC
AlwNI 763 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2007 CAGCTACTG
BsaHI 747 GACGTC G[GA]^CG[CT]C
883 GGCGCC
BsaI 1642 GAGACC GGTCTC(1/5)
1878 GGTCTC
BsrFI 60 GCCGGC [GA]^CCGG[CT]
327 GCCGGT
BstEII 771 GGTGACC G^GT[ACGT]ACC
2361 GGTCACC
BstPI 771 GGTGACC G^GT[ACGT]ACC
2361 GGTCACC
Cfr10I 60 GCCGGC [GA]^CCGG[CT]
327 GCCGGT
DsaI 578 CCATGG C^C[GA][CT]GG
2096 CCACGG
EarI 112 CTCTTC CTCTTC(1/4)
2446 CTCTTC
EcoO65I 771 GGTGACC G^GT[ACGT]ACC
2361 GGTCACC
EcoT14I 578 CCATGG C^C[AT][AT]GG
1874 CCAAGG
Hin1I 747 GACGTC G[GA]^CG[CT]C
883 GGCGCC
Ksp632I 112 CTCTTC CTCTTC(1/4)
2446 CTCTTC
PflMI 762 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1027 CCACAGGCTGG
PleI 433 GAGTC GAGTC(4/5)
904 GAGTC
StyI 578 CCATGG C^C[AT][AT]GG
1874 CCAAGG
Tsp509I 2082 AATT ^AATT
2148 AATT
Van91I 762 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1027 CCACAGGCTGG
XcmI 870 CCAGAACTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1694 CCACCATCGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BglI
BmyI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TspRI
TthHB8I