Restriction map for
pFN21AB7319
(Linear) (Six-base) MAPSORT of: KIBB7319.seq Check: 7506 from: 1 to: 2517
KIBB7319.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 153 1234 1894 2517
Size: 153 1081 660 623
Fragments arranged by size:
1081 660 623 153
AatII G_ACGT'C
Cuts at: 0 751 2517
Size: 751 1766
AccI GT'mk_AC
Cuts at: 0 1154 1244 2517
Size: 1154 90 1273
Fragments arranged by size:
1273 1154 90
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1444 1607 2517
Size: 987 457 163 910
Fragments arranged by size:
987 910 457 163
AleI CACnn'nnGTG
Cuts at: 0 2368 2517
Size: 2368 149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 280 2197 2269 2517
Size: 280 1917 72 248
Fragments arranged by size:
1917 280 248 72
AlwNI CAG_nnn'CTG
Cuts at: 0 768 2012 2517
Size: 768 1244 505
Fragments arranged by size:
1244 768 505
ApaI G_GGCC'C
Cuts at: 0 8 1027 1804 2033 2517
Size: 8 1019 777 229 484
Fragments arranged by size:
1019 777 484 229 8
AvaI C'yCGr_G
Cuts at: 0 996 2517
Size: 996 1521
BanI G'GyrC_C
Cuts at: 0 165 883 958 1493 1567 1689 2517
Size: 165 718 75 535 74 122 828
Fragments arranged by size:
828 718 535 165 122 75 74
BanII G_rGCy'C
Cuts at: 0 8 1027 1804 2033 2517
Size: 8 1019 777 229 484
Fragments arranged by size:
1019 777 484 229 8
BbeI G_GCGC'C
Cuts at: 0 887 2517
Size: 887 1630
BbsI GAAGACnn'nnnn_
Cuts at: 0 1662 2517
Size: 1662 855
BbvCI CC'TCA_GC
Cuts at: 0 1048 1732 2290 2517
Size: 1048 684 558 227
Fragments arranged by size:
1048 684 558 227
BglI GCCn_nnn'nGGC
Cuts at: 0 956 1208 1565 1714 2517
Size: 956 252 357 149 803
Fragments arranged by size:
956 803 357 252 149
BglII A'GATC_T
Cuts at: 0 1268 2517
Size: 1268 1249
BlpI GC'TnA_GC
Cuts at: 0 56 2517
Size: 56 2461
Bme1580I G_kGCm'C
Cuts at: 0 8 170 961 992 1027 1804 2033 2517
Size: 8 162 791 31 35 777 229 484
Fragments arranged by size:
791 777 484 229 162 35 31 8
BmgBI CAC'GTC
Cuts at: 0 1329 1533 2517
Size: 1329 204 984
Fragments arranged by size:
1329 984 204
BmrI ACTGGGnnnn_n'
Cuts at: 0 2022 2517
Size: 2022 495
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1782 2517
Size: 1782 735
Bpu10I CC'TnA_GC
Cuts at: 0 1048 1732 2290 2517
Size: 1048 684 558 227
Fragments arranged by size:
1048 684 558 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 2517
Size: 1282 1235
BsaI GGTCTCn'nnnn_
Cuts at: 0 1636 1884 2517
Size: 1636 248 633
Fragments arranged by size:
1636 633 248
BsaAI yAC'GTr
Cuts at: 0 1863 2517
Size: 1863 654
BsaHI Gr'CG_yC
Cuts at: 0 748 884 2517
Size: 748 136 1633
Fragments arranged by size:
1633 748 136
BsaWI w'CCGG_w
Cuts at: 0 1367 2517
Size: 1367 1150
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 445 475 2517
Size: 445 30 2042
Fragments arranged by size:
2042 445 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 15 253 769 1195 1279 2517
Size: 15 238 516 426 84 1238
Fragments arranged by size:
1238 516 426 238 84 15
BseYI C'CCAG_C
Cuts at: 0 279 553 1033 1795 2517
Size: 279 274 480 762 722
Fragments arranged by size:
762 722 480 279 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 604 913 1180 1212 1321 2313 2517
Size: 580 24 309 267 32 109 992 204
Fragments arranged by size:
992 580 309 267 204 109 32 24
BsiHKAI G_wGCw'C
Cuts at: 0 335 802 2393 2517
Size: 335 467 1591 124
Fragments arranged by size:
1591 467 335 124
BsmI GAATG_Cn'
Cuts at: 0 1606 2517
Size: 1606 911
BsmBI CGTCTCn'nnnn_
Cuts at: 0 706 2043 2325 2517
Size: 706 1337 282 192
Fragments arranged by size:
1337 706 282 192
Bsp1286I G_dGCh'C
Cuts at: 0 8 170 335 802 961 992 1027 1804
Size: 8 162 165 467 159 31 35 777
Cuts at: 1804 2033 2393 2517
Size: 229 360 124
Fragments arranged by size:
777 467 360 229 165 162 159 124
35 31 8
BspHI T'CATG_A
Cuts at: 0 440 2517
Size: 440 2077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 153 1081 1234 1894 2213 2517
Size: 92 61 928 153 660 319 304
Fragments arranged by size:
928 660 319 304 153 92 61
BsrDI GCAATG_nn'
Cuts at: 0 1366 2517
Size: 1366 1151
BsrFI r'CCGG_y
Cuts at: 0 60 327 2517
Size: 60 267 2190
Fragments arranged by size:
2190 267 60
BssHII G'CGCG_C
Cuts at: 0 805 2517
Size: 805 1712
BssSI C'ACGA_G
Cuts at: 0 349 2517
Size: 349 2168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 222 591 1556 2517
Size: 222 369 965 961
Fragments arranged by size:
965 961 369 222
BstEII G'GTnAC_C
Cuts at: 0 771 2361 2517
Size: 771 1590 156
Fragments arranged by size:
1590 771 156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 667 1239 1704 2412 2517
Size: 667 572 465 708 105
Fragments arranged by size:
708 667 572 465 105
BstYI r'GATC_y
Cuts at: 0 1268 2517
Size: 1268 1249
BtgI C'CryG_G
Cuts at: 0 578 2096 2517
Size: 578 1518 421
Fragments arranged by size:
1518 578 421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 518 684 1683 2517
Size: 518 166 999 834
Fragments arranged by size:
999 834 518 166
BtsI GCAGTG_nn'
Cuts at: 0 724 1825 2517
Size: 724 1101 692
Fragments arranged by size:
1101 724 692
DrdI GACnn_nn'nnGTC
Cuts at: 0 2380 2517
Size: 2380 137
EaeI y'GGCC_r
Cuts at: 0 449 645 657 1715 1742 1982 2093 2402
Size: 449 196 12 1058 27 240 111 309
Cuts at: 2402 2517
Size: 115
Fragments arranged by size:
1058 449 309 240 196 115 111 27
12
EarI CTCTTCn'nnn_
Cuts at: 0 118 2452 2517
Size: 118 2334 65
Fragments arranged by size:
2334 118 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 1444 1607 1782 2517
Size: 987 457 163 175 735
Fragments arranged by size:
987 735 457 175 163
EcoNI CCTnn'n_nnAGG
Cuts at: 0 214 2517
Size: 214 2303
EcoO109I rG'GnC_Cy
Cuts at: 0 4 48 1023 1800 1801 1902 2029 2030
Size: 4 44 975 777 1 101 127 1
Cuts at: 2030 2517
Size: 487
Fragments arranged by size:
975 777 487 127 101 44 4 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 543 1299 2517
Size: 543 756 1218
Fragments arranged by size:
1218 756 543
HaeII r_GCGC'y
Cuts at: 0 497 560 762 768 887 2517
Size: 497 63 202 6 119 1630
Fragments arranged by size:
1630 497 202 119 63 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1362 1394 2517
Size: 1362 32 1123
Fragments arranged by size:
1362 1123 32
HincII GTy'rAC
Cuts at: 0 1281 2517
Size: 1281 1236
KasI G'GCGC_C
Cuts at: 0 883 2517
Size: 883 1634
MfeI C'AATT_G
Cuts at: 0 2147 2517
Size: 2147 370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 2517
Size: 338 2179
MscI TGG'CCA
Cuts at: 0 451 659 1744 1984 2095 2404 2517
Size: 451 208 1085 240 111 309 113
Fragments arranged by size:
1085 451 309 240 208 113 111
MslI CAynn'nnrTG
Cuts at: 0 445 1702 1750 2335 2368 2517
Size: 445 1257 48 585 33 149
Fragments arranged by size:
1257 585 445 149 48 33
MspA1I CmG'CkG
Cuts at: 0 588 605 663 1257 1422 1665 2351 2517
Size: 588 17 58 594 165 243 686 166
Fragments arranged by size:
686 594 588 243 166 165 58 17
NaeI GCC'GGC
Cuts at: 0 62 2517
Size: 62 2455
NarI GG'CG_CC
Cuts at: 0 884 2517
Size: 884 1633
NcoI C'CATG_G
Cuts at: 0 578 2517
Size: 578 1939
NgoMIV G'CCGG_C
Cuts at: 0 60 2517
Size: 60 2457
NspI r_CATG'y
Cuts at: 0 2091 2517
Size: 2091 426
PasI CC'CwG_GG
Cuts at: 0 8 1722 2517
Size: 8 1714 795
Fragments arranged by size:
1714 795 8
PflMI CCAn_nnn'nTGG
Cuts at: 0 768 1033 2517
Size: 768 265 1484
Fragments arranged by size:
1484 768 265
PfoI T'CCnGG_A
Cuts at: 0 707 1292 1673 2517
Size: 707 585 381 844
Fragments arranged by size:
844 707 585 381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2263 2295 2517
Size: 2263 32 222
Fragments arranged by size:
2263 222 32
PpuMI rG'GwC_Cy
Cuts at: 0 48 2517
Size: 48 2469
PspOMI G'GGCC_C
Cuts at: 0 4 1023 1800 2029 2517
Size: 4 1019 777 229 488
Fragments arranged by size:
1019 777 488 229 4
PstI C_TGCA'G
Cuts at: 0 164 731 794 1415 2517
Size: 164 567 63 621 1102
Fragments arranged by size:
1102 621 567 164 63
PvuII CAG'CTG
Cuts at: 0 588 663 1257 1422 1665 2517
Size: 588 75 594 165 243 852
Fragments arranged by size:
852 594 588 243 165 75
SbfI CC_TGCA'GG
Cuts at: 0 164 2517
Size: 164 2353
SfcI C'TryA_G
Cuts at: 0 160 381 489 727 790 1155 1411 2004
Size: 160 221 108 238 63 365 256 593
Cuts at: 2004 2346 2517
Size: 342 171
Fragments arranged by size:
593 365 342 256 238 221 171 160
108 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1714 2517
Size: 1714 803
SfoI GGC'GCC
Cuts at: 0 885 2517
Size: 885 1632
SmaI CCC'GGG
Cuts at: 0 998 2517
Size: 998 1519
SmlI C'TyrA_G
Cuts at: 0 1261 2517
Size: 1261 1256
SphI G_CATG'C
Cuts at: 0 2091 2517
Size: 2091 426
StuI AGG'CCT
Cuts at: 0 1609 2517
Size: 1609 908
StyI C'CwwG_G
Cuts at: 0 578 1874 2517
Size: 578 1296 643
Fragments arranged by size:
1296 643 578
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 567 1707 1794 2517
Size: 94 473 1140 87 723
Fragments arranged by size:
1140 723 473 94 87
TatI w'GTAC_w
Cuts at: 0 1163 2517
Size: 1163 1354
Tth111I GACn'n_nGTC
Cuts at: 0 922 2517
Size: 922 1595
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 877 1701 2517
Size: 877 824 816
Fragments arranged by size:
877 824 816
XmaI C'CCGG_G
Cuts at: 0 996 2517
Size: 996 1521
XmnI GAAnn'nnTTC
Cuts at: 0 1381 2517
Size: 1381 1136
ZraI GAC'GTC
Cuts at: 0 749 2517
Size: 749 1768
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SbfI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BmtI BplI BsaBI BsiEI
BsiWI BspEI BsrBI BsrGI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI ScaI SexAI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TstI XbaI XhoI