################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV FbaI FseI FspI Hin1I HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 486 GGTACC G^GTACC Aor51HI 3382 AGCGCT AGC^GCT Asp718I 486 GGTACC G^GTACC BamHI 1717 GGATCC G^GATCC BanI 486 GGTACC G^G[CT][GA]CC BciVI 3144 GGATAC GTATCC(6/5) BsaAI 1678 TACGTG [CT]AC^GT[GA] BsmFI 1683 GGGAC GGGAC(10/14) Bsp1407I 598 TGTACA T^GTACA BsrGI 598 TGTACA T^GTACA Bst1107I 672 GTATAC GTA^TAC BstZ17I 672 GTATAC GTA^TAC CfoI 3383 GCGC GCG^C DraII 425 AGGTCCT [GA]G^G[ACGT]CC[CT] EarI 2164 CTCTTC CTCTTC(1/4) Eco47III 3382 AGCGCT AGC^GCT EcoO109I 425 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 2871 GAATTC G^AATTC FauI 22 CCCGC CCCGC(4/6) HaeII 3382 AGCGCT [GA]GCGC^[CT] HapII 1279 CCGG C^CGG HgaI 730 GCGTC GACGC(5/10) HhaI 3383 GCGC GCG^C HinP1I 3383 GCGC G^CGC HpaII 1279 CCGG C^CGG KpnI 486 GGTACC GGTAC^C Ksp632I 2164 CTCTTC CTCTTC(1/4) MspI 1279 CCGG C^CGG NheI 3235 GCTAGC G^CTAGC PpuMI 425 AGGTCCT [GA]G^G[AT]CC[CT] PvuII 332 CAGCTG CAG^CTG ScaI 1441 AGTACT AGT^ACT SmlI 3119 CTCAAG C^T[CT][GA]AG SspBI 598 TGTACA T^GTACA StuI 3164 AGGCCT AGG^CCT TaqI 3220 TCGA T^CGA TthHB8I 3220 TCGA T^CGA XbaI 1078 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccI 672 GTATAC GT^[AC][GT]AC 2945 GTAGAC BanII 19 GAGCCC G[GA]GC[CT]^C 3214 GGGCTC BsaBI 435 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1837 GATATGGATC BsgI 1702 GTGCAG GTGCAG(16/14) 2384 GTGCAG BspMI 2421 ACCTGC ACCTGC(4/8) 3566 GCAGGT DraI 2620 TTTAAA TTT^AAA 3183 TTTAAA DraIII 1360 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG 2644 CACTTTGTG EcoNI 355 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2759 CCTATGTAAGG EcoT22I 784 ATGCAT ATGCA^T 3367 ATGCAT HincII 2787 GTTGAC GT[CT]^[GA]AC 3204 GTCAAC HindII 2787 GTTGAC GT[CT]^[GA]AC 3204 GTCAAC MaeII 410 ACGT A^CGT 1679 ACGT MamI 435 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1837 GATATGGATC NdeI 1211 CATATG CA^TATG 1471 CATATG NsiI 784 ATGCAT ATGCA^T 3367 ATGCAT PstI 335 CTGCAG CTGCA^G 2423 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BalI BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspHI BspLU11I BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmnI