(Linear) (Six-base) MAPSORT of: KIBB7321.seq Check: 8291 from: 1 to: 3597 KIBB7321.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2430 3597 Size: 2430 1167 AccI GT'mk_AC Cuts at: 0 673 2946 3597 Size: 673 2273 651 Fragments arranged by size: 2273 673 651 Acc65I G'GTAC_C Cuts at: 0 486 3597 Size: 486 3111 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 451 1129 2566 3597 Size: 451 678 1437 1031 Fragments arranged by size: 1437 1031 678 451 AfeI AGC'GCT Cuts at: 0 3384 3597 Size: 3384 213 AflIII A'CryG_T Cuts at: 0 408 886 2595 2783 3597 Size: 408 478 1709 188 814 Fragments arranged by size: 1709 814 478 408 188 AleI CACnn'nnGTG Cuts at: 0 1473 3597 Size: 1473 2124 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3094 3126 3597 Size: 3094 32 471 Fragments arranged by size: 3094 471 32 ApoI r'AATT_y Cuts at: 0 114 549 613 865 878 1115 1399 1588 Size: 114 435 64 252 13 237 284 189 Cuts at: 1588 1921 2011 2118 2871 3597 Size: 333 90 107 753 726 Fragments arranged by size: 753 726 435 333 284 252 237 189 114 107 90 64 13 BamHI G'GATC_C Cuts at: 0 1717 3597 Size: 1717 1880 BanI G'GyrC_C Cuts at: 0 486 3597 Size: 486 3111 BanII G_rGCy'C Cuts at: 0 23 3218 3597 Size: 23 3195 379 Fragments arranged by size: 3195 379 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 466 912 2257 3597 Size: 466 446 1345 1340 Fragments arranged by size: 1345 1340 466 446 BciVI GTATCCnnnnn_n' Cuts at: 0 3138 3597 Size: 3138 459 BfrBI ATG'CAT Cuts at: 0 786 3369 3597 Size: 786 2583 228 Fragments arranged by size: 2583 786 228 BmrI ACTGGGnnnn_n' Cuts at: 0 485 3297 3597 Size: 485 2812 300 Fragments arranged by size: 2812 485 300 BmtI G_CTAG'C Cuts at: 0 3239 3597 Size: 3239 358 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 539 3597 Size: 539 3058 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2006 2913 3194 3534 3597 Size: 2006 907 281 340 63 Fragments arranged by size: 2006 907 340 281 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3104 3597 Size: 3104 493 BsaAI yAC'GTr Cuts at: 0 1680 3597 Size: 1680 1917 BsaBI GATnn'nnATC Cuts at: 0 439 1841 3597 Size: 439 1402 1756 Fragments arranged by size: 1756 1402 439 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 655 685 886 916 1817 1847 3597 Size: 655 30 201 30 901 30 1750 Fragments arranged by size: 1750 901 655 201 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1204 3142 3145 3597 Size: 1204 1938 3 452 Fragments arranged by size: 1938 1204 452 3 BseYI C'CCAG_C Cuts at: 0 1052 3260 3597 Size: 1052 2208 337 Fragments arranged by size: 2208 1052 337 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1723 2405 3597 Size: 1723 682 1192 Fragments arranged by size: 1723 1192 682 BsiHKAI G_wGCw'C Cuts at: 0 525 2134 3461 3597 Size: 525 1609 1327 136 Fragments arranged by size: 1609 1327 525 136 Bsp1286I G_dGCh'C Cuts at: 0 23 525 2134 3218 3461 3597 Size: 23 502 1609 1084 243 136 Fragments arranged by size: 1609 1084 502 243 136 23 BspHI T'CATG_A Cuts at: 0 678 852 3153 3597 Size: 678 174 2301 444 Fragments arranged by size: 2301 678 444 174 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2430 3557 3597 Size: 2430 1127 40 Fragments arranged by size: 2430 1127 40 BsrDI GCAATG_nn' Cuts at: 0 638 823 1859 2273 2728 2841 3597 Size: 638 185 1036 414 455 113 756 Fragments arranged by size: 1036 756 638 455 414 185 113 BsrGI T'GTAC_A Cuts at: 0 598 3597 Size: 598 2999 BstAPI GCAn_nnn'nTGC Cuts at: 0 343 637 1858 2113 3597 Size: 343 294 1221 255 1484 Fragments arranged by size: 1484 1221 343 294 255 BstYI r'GATC_y Cuts at: 0 774 1717 1842 3597 Size: 774 943 125 1755 Fragments arranged by size: 1755 943 774 125 BstZ17I GTA'TAC Cuts at: 0 674 3597 Size: 674 2923 BtsI GCAGTG_nn' Cuts at: 0 1567 1732 2071 3597 Size: 1567 165 339 1526 Fragments arranged by size: 1567 1526 339 165 DraI TTT'AAA Cuts at: 0 2622 3185 3597 Size: 2622 563 412 Fragments arranged by size: 2622 563 412 DraIII CAC_nnn'GTG Cuts at: 0 1365 2649 3597 Size: 1365 1284 948 Fragments arranged by size: 1365 1284 948 EaeI y'GGCC_r Cuts at: 0 746 2405 2640 3229 3597 Size: 746 1659 235 589 368 Fragments arranged by size: 1659 746 589 368 235 EarI CTCTTCn'nnn_ Cuts at: 0 2170 3597 Size: 2170 1427 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 451 1129 2006 2566 2913 3194 3534 3597 Size: 451 678 877 560 347 281 340 63 Fragments arranged by size: 877 678 560 451 347 340 281 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 359 2763 3597 Size: 359 2404 834 Fragments arranged by size: 2404 834 359 EcoO109I rG'GnC_Cy Cuts at: 0 426 3597 Size: 426 3171 EcoRI G'AATT_C Cuts at: 0 2871 3597 Size: 2871 726 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 490 898 936 1378 2181 3597 Size: 490 408 38 442 803 1416 Fragments arranged by size: 1416 803 490 442 408 38 HaeII r_GCGC'y Cuts at: 0 3386 3597 Size: 3386 211 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 212 244 571 603 1642 1674 3354 3386 Size: 212 32 327 32 1039 32 1680 32 Cuts at: 3386 3597 Size: 211 Fragments arranged by size: 1680 1039 327 212 211 32 32 32 32 HincII GTy'rAC Cuts at: 0 2789 3206 3597 Size: 2789 417 391 Fragments arranged by size: 2789 417 391 KpnI G_GTAC'C Cuts at: 0 490 3597 Size: 490 3107 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3072 3597 Size: 3072 525 MscI TGG'CCA Cuts at: 0 748 2407 2642 3597 Size: 748 1659 235 955 Fragments arranged by size: 1659 955 748 235 MslI CAynn'nnrTG Cuts at: 0 413 1473 2173 3452 3509 3597 Size: 413 1060 700 1279 57 88 Fragments arranged by size: 1279 1060 700 413 88 57 MspA1I CmG'CkG Cuts at: 0 6 334 3376 3597 Size: 6 328 3042 221 Fragments arranged by size: 3042 328 221 6 NdeI CA'TA_TG Cuts at: 0 1212 1472 3597 Size: 1212 260 2125 Fragments arranged by size: 2125 1212 260 NheI G'CTAG_C Cuts at: 0 3235 3597 Size: 3235 362 NsiI A_TGCA'T Cuts at: 0 788 3371 3597 Size: 788 2583 226 Fragments arranged by size: 2583 788 226 NspI r_CATG'y Cuts at: 0 786 890 2599 2787 3192 3597 Size: 786 104 1709 188 405 405 Fragments arranged by size: 1709 786 405 405 188 104 PciI A'CATG_T Cuts at: 0 886 2595 2783 3597 Size: 886 1709 188 814 Fragments arranged by size: 1709 886 814 188 PflMI CCAn_nnn'nTGG Cuts at: 0 1047 2649 2974 3597 Size: 1047 1602 325 623 Fragments arranged by size: 1602 1047 623 325 PfoI T'CCnGG_A Cuts at: 0 1662 3597 Size: 1662 1935 PpuMI rG'GwC_Cy Cuts at: 0 426 3597 Size: 426 3171 PsiI TTA'TAA Cuts at: 0 257 3597 Size: 257 3340 PstI C_TGCA'G Cuts at: 0 339 2427 3597 Size: 339 2088 1170 Fragments arranged by size: 2088 1170 339 PvuII CAG'CTG Cuts at: 0 334 3597 Size: 334 3263 ScaI AGT'ACT Cuts at: 0 1443 3597 Size: 1443 2154 SfcI C'TryA_G Cuts at: 0 335 722 2073 2245 2423 3413 3597 Size: 335 387 1351 172 178 990 184 Fragments arranged by size: 1351 990 387 335 184 178 172 SmlI C'TyrA_G Cuts at: 0 3119 3597 Size: 3119 478 StuI AGG'CCT Cuts at: 0 3166 3597 Size: 3166 431 StyI C'CwwG_G Cuts at: 0 67 269 743 1046 3597 Size: 67 202 474 303 2551 Fragments arranged by size: 2551 474 303 202 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1092 3095 3303 3597 Size: 1092 2003 208 294 Fragments arranged by size: 2003 1092 294 208 TatI w'GTAC_w Cuts at: 0 598 1441 2339 2921 3597 Size: 598 843 898 582 676 Fragments arranged by size: 898 843 676 598 582 XbaI T'CTAG_A Cuts at: 0 1078 3597 Size: 1078 2519 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 398 1060 2001 3597 Size: 398 662 941 1596 Fragments arranged by size: 1596 941 662 398 XmnI GAAnn'nnTTC Cuts at: 0 697 878 2522 3597 Size: 697 181 1644 1075 Fragments arranged by size: 1644 1075 697 181 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AleI AloI ApoI BamHI BanI BanII BbsI BciVI BfrBI BmrI BmtI BplI BpmI BpuEI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI BstYI BstZ17I BtsI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NdeI NheI NsiI NspI PciI PflMI PfoI PpuMI PsiI PstI PvuII ScaI SfcI SmlI StuI StyI TaqII TatI XbaI XcmI XmnI Enzymes that do not cut: AatII AclI AflII AgeI AhdI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI Bpu10I BsaI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NgoMIV NotI NruI PacI PasI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI XmaI ZraI