(Circular) (Six-base) MAPSORT of: pFN21AB7778.seq Check: 9460 from: 1 to: 6229 pFN21AB7778 6229 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3319 3319 Size: 6229 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3194 4039 278 Size: 53 83 186 936 1658 845 2468 Fragments arranged by size: 2468 1658 936 845 186 83 53 AccI GT'mk_AC Cuts at: 1937 2588 3572 1937 Size: 651 984 4594 Fragments arranged by size: 4594 984 651 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2318 2470 2813 2993 4292 5676 5944 1632 Size: 686 152 343 180 1299 1384 268 1917 Fragments arranged by size: 1917 1384 1299 686 343 268 180 152 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 5128 6105 1203 Size: 3925 977 1327 Fragments arranged by size: 3925 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 2254 4958 2254 Size: 2704 3525 AleI CACnn'nnGTG Cuts at: 1166 3209 1166 Size: 2043 4186 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6229 AlwNI CAG_nnn'CTG Cuts at: 2554 2924 3972 5544 2554 Size: 370 1048 1572 3239 Fragments arranged by size: 3239 1572 1048 370 ApaI G_GGCC'C Cuts at: 2163 2227 2346 2163 Size: 64 119 6046 Fragments arranged by size: 6046 119 64 ApaLI G'TGCA_C Cuts at: 3089 4286 5442 3089 Size: 1197 1156 3876 Fragments arranged by size: 3876 1197 1156 ApoI r'AATT_y Cuts at: 1493 3538 3764 3916 6025 1493 Size: 2045 226 152 2109 1697 Fragments arranged by size: 2109 2045 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2138 2156 2345 2424 3265 3554 1870 Size: 73 195 18 189 79 841 289 4545 Fragments arranged by size: 4545 841 289 195 189 79 73 18 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2209 2279 2914 3313 3550 Size: 325 217 627 422 70 635 399 237 Cuts at: 3550 5005 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 635 627 422 399 325 237 217 70 BanII G_rGCy'C Cuts at: 1391 2051 2157 2163 2227 2346 3548 1391 Size: 660 106 6 64 119 1202 4072 Fragments arranged by size: 4072 1202 660 119 106 64 6 BbeI G_GCGC'C Cuts at: 2213 2283 2213 Size: 70 6159 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2426 2504 928 Size: 971 527 78 4653 Fragments arranged by size: 4653 971 527 78 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4420 4454 1903 Size: 34 2483 34 3678 Fragments arranged by size: 3678 2483 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 2619 1548 Size: 1071 5158 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 4840 136 Size: 107 122 71 631 605 361 2807 1525 Fragments arranged by size: 2807 1525 631 605 361 122 107 71 BglII A'GATC_T Cuts at: 2737 6224 2737 Size: 3487 2742 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 2163 2227 2346 3093 3204 4290 5446 1790 Size: 373 64 119 747 111 1086 1156 2573 Fragments arranged by size: 2573 1156 1086 747 373 119 111 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2934 3065 3960 Size: 416 53 83 399 24 1517 131 895 Cuts at: 3960 4918 442 Size: 958 1753 Fragments arranged by size: 1753 1517 958 895 416 399 131 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2266 2266 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 3139 4889 1239 Size: 953 947 1750 2579 Fragments arranged by size: 2579 1750 953 947 Bpu10I CC'TnA_GC Cuts at: 2309 2309 Size: 6229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4354 5219 5517 4354 Size: 865 298 5066 Fragments arranged by size: 5066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2624 2771 4892 882 Size: 617 1125 147 2121 2219 Fragments arranged by size: 2219 2121 1125 617 147 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 2321 6223 2321 Size: 3902 2327 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2210 2280 2351 3191 Size: 53 83 186 936 677 70 71 840 Cuts at: 3191 3363 4036 4418 5053 275 Size: 172 673 382 635 1451 Fragments arranged by size: 1451 936 840 677 673 635 382 186 172 83 71 70 53 BsaWI w'CCGG_w Cuts at: 2073 4662 5060 5334 5481 5892 2073 Size: 2589 398 274 147 411 2410 Fragments arranged by size: 2589 2410 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6004 Fragments arranged by size: 6004 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2279 2282 2285 2989 3142 1186 Size: 1093 3 3 704 153 4273 Fragments arranged by size: 4273 1093 704 153 3 3 BseYI C'CCAG_C Cuts at: 2021 2275 3060 5432 5928 2021 Size: 254 785 2372 496 2322 Fragments arranged by size: 2372 2322 785 496 254 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3095 3422 1163 Size: 573 1359 327 3970 Fragments arranged by size: 3970 1359 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2093 2167 3679 4440 5468 1540 Size: 275 186 92 74 1512 761 1028 2301 Fragments arranged by size: 2301 1512 1028 761 275 186 92 74 BsiHKAI G_wGCw'C Cuts at: 3093 4290 4375 5446 3093 Size: 1197 85 1071 3876 Fragments arranged by size: 3876 1197 1071 85 BsmI GAATG_Cn' Cuts at: 3304 3740 3833 3304 Size: 436 93 5700 Fragments arranged by size: 5700 436 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3117 3117 Size: 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2157 2163 2227 2346 3093 3204 Size: 399 261 106 6 64 119 747 111 Cuts at: 3204 3548 4290 4375 5446 1391 Size: 344 742 85 1071 2174 Fragments arranged by size: 2174 1071 747 742 399 344 261 119 111 106 85 64 6 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 2508 3186 4118 5848 2508 Size: 678 932 1730 2889 Fragments arranged by size: 2889 1730 932 678 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 2826 3055 3319 3584 844 Size: 1819 163 229 264 265 3489 Fragments arranged by size: 3489 1819 265 264 229 163 BsrBI CCG'CTC Cuts at: 1394 1823 2176 2287 2388 2400 4116 1394 Size: 429 353 111 101 12 1716 3507 Fragments arranged by size: 3507 1716 429 353 111 101 12 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2380 2633 2797 4873 5060 1700 Size: 110 570 253 164 2076 187 2869 Fragments arranged by size: 2869 2076 570 253 187 164 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstXI CCAn_nnnn'nTGG Cuts at: 2911 3211 2911 Size: 300 5929 BstYI r'GATC_y Cuts at: 1972 2737 3405 3559 3926 4311 4328 5780 5866 Size: 765 668 154 367 385 17 1452 86 Cuts at: 5866 5878 6224 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 765 668 385 367 346 154 86 17 12 BstZ17I GTA'TAC Cuts at: 2589 2589 Size: 6229 BtgI C'CryG_G Cuts at: 513 1065 2028 2217 513 Size: 552 963 189 4525 Fragments arranged by size: 4525 963 552 189 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 2776 2959 3060 3745 4539 4559 2776 Size: 183 101 685 794 20 4446 Fragments arranged by size: 4446 794 685 183 101 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2707 2742 529 Size: 35 2143 35 4016 Fragments arranged by size: 4016 2143 35 35 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2090 2164 2361 Size: 54 1425 50 275 18 260 74 197 Cuts at: 2361 2565 3217 3676 4565 6194 8 Size: 204 652 459 889 1629 43 Fragments arranged by size: 1629 1425 889 652 459 275 260 204 197 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2090 2164 3676 1537 Size: 275 278 74 1512 4090 Fragments arranged by size: 4090 1512 278 275 74 EarI CTCTTCn'nnn_ Cuts at: 1353 2724 2969 4159 1353 Size: 1371 245 1190 3423 Fragments arranged by size: 3423 1371 1190 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2318 2470 2813 2993 3139 4292 Size: 393 560 126 152 343 180 146 1153 Cuts at: 4292 4889 5676 5944 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1153 787 597 560 393 343 268 180 152 146 126 EcoO109I rG'GnC_Cy Cuts at: 2134 2223 2224 3007 3067 3097 2134 Size: 89 1 783 60 30 5266 Fragments arranged by size: 5266 783 89 60 30 1 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 2213 2283 2476 5376 1678 Size: 535 70 193 2900 2531 Fragments arranged by size: 2900 2531 535 193 70 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 4870 4902 4944 Size: 32 135 32 668 32 2598 32 42 Cuts at: 4944 4976 1373 Size: 32 2626 Fragments arranged by size: 2626 2598 668 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3573 3819 669 Size: 988 281 1635 246 3079 Fragments arranged by size: 3079 1635 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KasI G'GCGC_C Cuts at: 2209 2279 2209 Size: 70 6159 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2736 5343 5527 1305 Size: 1431 2607 184 2007 Fragments arranged by size: 2607 2007 1431 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2567 3219 10 Size: 54 1425 343 735 652 3020 Fragments arranged by size: 3020 1425 735 652 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3038 3086 3185 3209 3329 4187 4546 Size: 648 1872 48 99 24 120 858 359 Cuts at: 4546 4705 518 Size: 159 2042 Fragments arranged by size: 2042 1872 858 648 359 159 120 99 48 24 MspA1I CmG'CkG Cuts at: 1606 2030 2192 2219 2235 2360 2883 2988 3019 Size: 424 162 27 16 125 523 105 31 Cuts at: 3019 3159 3645 4322 5470 5715 1606 Size: 140 486 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 523 486 424 245 162 140 125 105 31 27 16 NaeI GCC'GGC Cuts at: 2799 2799 Size: 6229 NarI GG'CG_CC Cuts at: 2210 2280 2210 Size: 70 6159 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5677 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NgoMIV G'CCGG_C Cuts at: 2797 2797 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3245 3587 5132 1132 Size: 97 2016 342 1545 2229 Fragments arranged by size: 2229 2016 1545 342 97 PasI CC'CwG_GG Cuts at: 1348 2056 1348 Size: 708 5521 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 3436 1108 Size: 2328 3901 PfoI T'CCnGG_A Cuts at: 3348 3348 Size: 6229 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2134 3067 2134 Size: 933 5296 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PspOMI G'GGCC_C Cuts at: 2159 2223 2342 2159 Size: 64 119 6046 Fragments arranged by size: 6046 119 64 PstI C_TGCA'G Cuts at: 830 2613 3581 830 Size: 1783 968 3478 Fragments arranged by size: 3478 1783 968 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 3019 3019 Size: 6229 SacII CC_GC'GG Cuts at: 2031 2220 2031 Size: 189 6040 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SanDI GG'GwC_CC Cuts at: 2134 2134 Size: 6229 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SexAI A'CCwGG_T Cuts at: 2748 2748 Size: 6229 SfcI C'TryA_G Cuts at: 826 1045 2230 2609 3485 3577 4712 5393 5584 Size: 219 1185 379 876 92 1135 681 191 Cuts at: 5584 826 Size: 1471 Fragments arranged by size: 1471 1185 1135 876 681 379 219 191 92 SfoI GGC'GCC Cuts at: 2211 2281 2211 Size: 70 6159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 2140 2426 3556 1872 Size: 268 286 1130 4545 Fragments arranged by size: 4545 1130 286 268 SmlI C'TyrA_G Cuts at: 820 1017 1943 4333 5234 5496 820 Size: 197 926 2390 901 262 1553 Fragments arranged by size: 2390 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3245 3587 1132 Size: 2113 342 3774 Fragments arranged by size: 3774 2113 342 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 2519 1517 Size: 1002 5227 StyI C'CwwG_G Cuts at: 513 1065 2781 3668 513 Size: 552 1716 887 3074 Fragments arranged by size: 3074 1716 887 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3203 3263 4256 4273 4426 4611 5056 Size: 1255 1623 60 993 17 153 185 445 Cuts at: 5056 325 Size: 1498 Fragments arranged by size: 1623 1498 1255 993 445 185 153 60 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2978 4475 6187 Size: 274 80 33 51 1443 1001 1497 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 1712 1497 1443 1001 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6229 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3268 3356 3268 Size: 88 6141 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 2138 2424 3554 1870 Size: 268 286 1130 4545 Fragments arranged by size: 4545 1130 286 268 XmnI GAAnn'nnTTC Cuts at: 3302 4358 3302 Size: 1056 5173 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3192 4037 276 Size: 53 83 186 936 1658 845 2468 Fragments arranged by size: 2468 1658 936 845 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI