(Circular) (Six-base) MAPSORT of: pFN21AB6445.seq Check: 1724 from: 1 to: 5650 pFN21AB6445 5650 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2287 2569 2287 Size: 282 5368 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2307 2655 2907 2993 1937 Size: 370 348 252 86 4594 Fragments arranged by size: 4594 370 348 252 86 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 3713 5097 5365 1632 Size: 503 1578 1384 268 1917 Fragments arranged by size: 1917 1578 1384 503 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 2556 4549 5526 1203 Size: 1353 1993 977 1327 Fragments arranged by size: 1993 1353 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 2512 2732 4379 2512 Size: 220 1647 3783 Fragments arranged by size: 3783 1647 220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2066 2275 1184 Size: 882 209 4559 Fragments arranged by size: 4559 882 209 AlwNI CAG_nnn'CTG Cuts at: 3393 4965 3393 Size: 1572 4078 ApaI G_GGCC'C Cuts at: 2453 2453 Size: 5650 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2036 2531 2959 3185 3337 5446 1493 Size: 543 495 428 226 152 2109 1697 Fragments arranged by size: 2109 1697 543 495 428 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2318 2338 2362 2674 2975 1870 Size: 73 375 20 24 312 301 4545 Fragments arranged by size: 4545 375 312 301 73 24 20 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2287 2861 2971 4426 618 Size: 325 217 627 500 574 110 1455 1842 Fragments arranged by size: 1842 1455 627 574 500 325 217 110 BanII G_rGCy'C Cuts at: 1391 2090 2442 2453 2766 2894 2901 2969 1391 Size: 699 352 11 313 128 7 68 4072 Fragments arranged by size: 4072 699 352 313 128 68 11 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2511 928 Size: 971 612 4067 Fragments arranged by size: 4067 971 612 BbvCI CC'TCA_GC Cuts at: 2816 2816 Size: 5650 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2686 2720 3841 3875 1903 Size: 34 749 34 1121 34 3678 Fragments arranged by size: 3678 1121 749 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2020 4261 136 Size: 107 122 71 631 605 348 2241 1525 Fragments arranged by size: 2241 1525 631 605 348 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 2024 3067 2024 Size: 1043 4607 Bme1580I G_kGCm'C Cuts at: 1790 2283 2290 2453 3711 4867 1790 Size: 493 7 163 1258 1156 2573 Fragments arranged by size: 2573 1258 1156 493 163 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2293 3381 4339 Size: 416 53 83 399 24 876 1088 958 Cuts at: 4339 442 Size: 1753 Fragments arranged by size: 1753 1088 958 876 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2104 4310 1239 Size: 865 2206 2579 Fragments arranged by size: 2579 2206 865 Bpu10I CC'TnA_GC Cuts at: 2816 2816 Size: 5650 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3775 4640 4938 3775 Size: 865 298 4487 Fragments arranged by size: 4487 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4313 882 Size: 617 2814 2219 Fragments arranged by size: 2814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3441 493 Size: 711 2237 2702 Fragments arranged by size: 2702 2237 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2420 3457 3839 4474 Size: 53 83 186 936 887 1037 382 635 Cuts at: 4474 275 Size: 1451 Fragments arranged by size: 1451 1037 936 887 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 1178 Size: 30 165 30 1350 30 4045 Fragments arranged by size: 4045 1350 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 2450 1186 Size: 1117 147 4386 Fragments arranged by size: 4386 1117 147 BseYI C'CCAG_C Cuts at: 2446 4853 5349 2446 Size: 2407 496 2747 Fragments arranged by size: 2747 2407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2077 1163 Size: 573 341 4736 Fragments arranged by size: 4736 573 341 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 2636 2712 3711 3796 4867 2090 Size: 546 76 999 85 1071 2873 Fragments arranged by size: 2873 1071 999 546 85 76 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2283 2290 2442 2453 2636 2712 Size: 399 300 193 7 152 11 183 76 Cuts at: 2712 2766 2894 2901 2969 3711 3796 4867 1391 Size: 54 128 7 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 300 193 183 152 128 85 76 68 54 11 7 7 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 3539 5269 3539 Size: 1730 3920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2287 2569 2616 2667 2691 3005 844 Size: 1443 282 47 51 24 314 3489 Fragments arranged by size: 3489 1443 314 282 51 47 24 BsrBI CCG'CTC Cuts at: 1394 1823 2079 3537 1394 Size: 429 256 1458 3507 Fragments arranged by size: 3507 1458 429 256 BsrDI GCAATG_nn' Cuts at: 65 1248 2796 4145 4319 65 Size: 1183 1548 1349 174 1396 Fragments arranged by size: 1548 1396 1349 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2919 4294 4481 1700 Size: 110 1109 1375 187 2869 Fragments arranged by size: 2869 1375 1109 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstXI CCAn_nnnn'nTGG Cuts at: 2833 2833 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645 Size: 1008 367 385 17 1452 86 12 346 Cuts at: 5645 1972 Size: 1977 Fragments arranged by size: 1977 1452 1008 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2886 2886 Size: 5650 BtgI C'CryG_G Cuts at: 513 1065 2848 513 Size: 552 1783 3315 Fragments arranged by size: 3315 1783 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2382 487 Size: 683 56 1156 3755 Fragments arranged by size: 3755 1156 683 56 BtsI GCAGTG_nn' Cuts at: 2236 2407 3166 3960 3980 2236 Size: 171 759 794 20 3906 Fragments arranged by size: 3906 794 759 171 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5650 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3097 3986 5615 Size: 54 1425 50 275 18 1267 889 1629 Cuts at: 5615 8 Size: 43 Fragments arranged by size: 1629 1425 1267 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3580 1353 Size: 2227 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2088 2088 Size: 5650 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2104 2135 3713 4310 5097 5365 1239 Size: 393 472 31 1578 597 787 268 1524 Fragments arranged by size: 1578 1524 787 597 472 393 268 31 EcoNI CCTnn'n_nnAGG Cuts at: 2101 2101 Size: 5650 EcoO109I rG'GnC_Cy Cuts at: 2148 2812 2148 Size: 664 4986 EcoRI G'AATT_C Cuts at: 2036 2531 2959 3337 2036 Size: 495 428 378 4349 Fragments arranged by size: 4349 495 428 378 EcoRV GAT'ATC Cuts at: 2938 2938 Size: 5650 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 2718 2750 4291 Size: 32 135 32 926 32 188 32 1541 Cuts at: 4291 4323 4365 4397 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1541 926 188 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 2538 3249 2538 Size: 711 4939 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2664 4764 4948 1305 Size: 1359 2100 184 2007 Fragments arranged by size: 2100 2007 1359 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3828 Fragments arranged by size: 3828 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2561 2831 3608 3967 4126 518 Size: 648 1395 270 777 359 159 2042 Fragments arranged by size: 2042 1395 777 648 359 270 159 MspA1I CmG'CkG Cuts at: 1606 2265 2850 3066 3743 4891 5136 1606 Size: 659 585 216 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 659 585 245 216 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5098 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 3008 4553 1132 Size: 97 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 97 PasI CC'CwG_GG Cuts at: 1348 2518 1348 Size: 1170 4480 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5650 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2684 2716 3719 3751 5232 5264 2684 Size: 32 1003 32 1481 32 3070 Fragments arranged by size: 3070 1481 1003 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2148 2148 Size: 5650 PshAI GACnn'nnGTC Cuts at: 1613 2597 1613 Size: 984 4666 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PspOMI G'GGCC_C Cuts at: 2449 2449 Size: 5650 PstI C_TGCA'G Cuts at: 830 2688 3002 830 Size: 1858 314 3478 Fragments arranged by size: 3478 1858 314 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 SacI G_AGCT'C Cuts at: 2090 2090 Size: 5650 SacII CC_GC'GG Cuts at: 2851 2851 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 2164 3898 5610 2164 Size: 1734 1712 2204 Fragments arranged by size: 2204 1734 1712 SexAI A'CCwGG_T Cuts at: 2508 2508 Size: 5650 SfcI C'TryA_G Cuts at: 826 1045 2684 2998 4133 4814 5005 826 Size: 219 1639 314 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 681 314 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2320 2364 2977 1872 Size: 448 44 613 4545 Fragments arranged by size: 4545 613 448 44 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 3754 4655 4917 820 Size: 197 926 395 1416 901 262 1553 Fragments arranged by size: 1553 1416 926 901 395 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 2187 1517 Size: 670 4980 StyI C'CwwG_G Cuts at: 513 1065 2901 3089 513 Size: 552 1836 188 3074 Fragments arranged by size: 3074 1836 552 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2048 2082 2291 3677 3694 3847 4032 Size: 1255 468 34 209 1386 17 153 185 Cuts at: 4032 4477 325 Size: 445 1498 Fragments arranged by size: 1498 1386 1255 468 445 209 185 153 34 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2162 2838 3896 Size: 274 80 33 51 1443 185 676 1058 Cuts at: 3896 5608 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1058 676 274 185 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2753 2785 2854 2886 5610 5642 2753 Size: 32 69 32 2724 32 2761 Fragments arranged by size: 2761 2724 69 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 2338 1943 Size: 395 5255 XmaI C'CCGG_G Cuts at: 1870 2318 2362 2975 1870 Size: 448 44 613 4545 Fragments arranged by size: 4545 613 448 44 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SanDI SapI SfiI SfoI SrfI SwaI XcmI