Restriction map for
pFN21AE0301
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HphI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 117 CGCG CG^CG
AocI 1076 CCTTAGG CC^T[ACGT]AGG
AspHI 374 GAGCAC G[AT]GC[AT]^C
AspI 154 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 203 TGGCCA TGG^CCA
BbsI 1091 GAAGAC GAAGAC(2/6)
BpuAI 1091 GAAGAC GAAGAC(2/6)
BsaAI 796 TACGTG [CT]AC^GT[GA]
BsaWI 1041 ACCGGA [AT]^CCGG[AT]
BseRI 1271 CTCCTC GAGGAG(10/8)
BsgI 61 GTGCAG GTGCAG(16/14)
BsiHKAI 374 GAGCAC G[AT]GC[AT]^C
BsmBI 103 GAGACG CGTCTC(1/5)
BsmFI 160 GTCCC GGGAC(10/14)
BsmI 588 GCATTC GAATGC(1/-1)
Bsp1407I 1157 TGTACA T^GTACA
BspHI 941 TCATGA T^CATGA
BspMI 12 GCAGGT ACCTGC(4/8)
BsrDI 982 GCAATG GCAATG(2/0)
BsrGI 1157 TGTACA T^GTACA
BstAPI 12 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 117 CGCG CG^CG
Bsu36I 1076 CCTTAGG CC^T[ACGT]AGG
DrdI 412 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 719 CCGTGG C^C[GA][CT]GG
EaeI 203 TGGCCA [CT]^GGCC[GA]
EarI 143 CTCTTC CTCTTC(1/4)
Eco81I 1076 CCTTAGG CC^T[ACGT]AGG
FauI 118 GCGGG CCCGC(4/6)
HapII 1042 CCGG C^CGG
HgaI 105 GACGC GACGC(5/10)
HpaI 868 GTTAAC GTT^AAC
HpaII 1042 CCGG C^CGG
Ksp632I 143 CTCTTC CTCTTC(1/4)
MluNI 203 TGGCCA TGG^CCA
MscI 203 TGGCCA TGG^CCA
MslI 939 CATCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1042 CCGG C^CGG
MvnI 117 CGCG CG^CG
NdeI 393 CATATG CA^TATG
NheI 348 GCTAGC G^CTAGC
PflFI 154 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 941 TCATGA T^CATGA
ScaI 516 AGTACT AGT^ACT
SexAI 1395 ACCTGGT A^CC[AT]GGT
SspBI 1157 TGTACA T^GTACA
SspI 1279 AATATT AAT^ATT
TfiI 285 GAATC G^A[AT]TC
Tth111I 154 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflIII 1160 ACATGT A^C[GA][CT]GT
1290 ACATGT
Asp700I 285 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1252 GAACCATTTC
AvaI 847 CCCGAG C^[CT]CG[GA]G
1444 CCCGAG
BciVI 381 GGATAC GTATCC(6/5)
524 GTATCC
BmyI 374 GAGCAC G[AGT]GC[ACT]^C
440 GGGCAC
BpmI 593 CTCCAG CTGGAG(16/14)
416 CTGGAG
BsiYI 911 CCTGCAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
978 CCTAGCAATGG
BslI 911 CCTGCAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
978 CCTAGCAATGG
BsmAI 103 GAGAC GTCTC(1/5)
198 GAGAC
BsoBI 847 CCCGAG C^[CT]CG[GA]G
1444 CCCGAG
Bsp1286I 374 GAGCAC G[AGT]GC[ACT]^C
440 GGGCAC
BspLU11I 1160 ACATGT A^CATGT
1290 ACATGT
Cac8I 78 GCTGGC GC[ACGT]^[ACGT]GC
348 GCTAGC
DraII 49 AGGACCC [GA]G^G[ACGT]CC[CT]
907 GGGTCCT
EcoO109I 49 AGGACCC [GA]G^G[ACGT]CC[CT]
907 GGGTCCT
HincII 868 GTTAAC GT[CT]^[GA]AC
1164 GTCAAC
HindII 868 GTTAAC GT[CT]^[GA]AC
1164 GTCAAC
MaeII 155 ACGT A^CGT
797 ACGT
NspI 1160 ACATGT [GA]CATG^[CT]
1290 ACATGT
PleI 3 GAGTC GAGTC(4/5)
419 GAGTC
PpuMI 49 AGGACCC [GA]G^G[AT]CC[CT]
907 GGGTCCT
Tsp45I 477 GTCAC ^GT[GC]AC
1126 GTCAC
TspRI 377 CACTG CA[GC]TG[ACGT][ACGT]^
573 CAGTG
XmnI 285 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1252 GAACCATTTC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BfaI BsaJI BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI Tsp509I TthHB8I XhoII