Restriction map for
pFN21AE0301
(Linear) (Six-base) MAPSORT of: KIEE0301.seq Check: 4704 from: 1 to: 1527
KIEE0301.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3 1527
Size: 3 1524
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1527
Size: 799 728
AflIII A'CryG_T
Cuts at: 0 1160 1290 1527
Size: 1160 130 237
Fragments arranged by size:
1160 237 130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 961 1527
Size: 961 566
AlwNI CAG_nnn'CTG
Cuts at: 0 18 232 502 628 1396 1527
Size: 18 214 270 126 768 131
Fragments arranged by size:
768 270 214 131 126 18
AvaI C'yCGr_G
Cuts at: 0 847 1444 1527
Size: 847 597 83
Fragments arranged by size:
847 597 83
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 374 407 1527
Size: 374 33 1120
Fragments arranged by size:
1120 374 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1098 1527
Size: 1098 429
BciVI GTATCCnnnnn_n'
Cuts at: 0 375 535 1527
Size: 375 160 992
Fragments arranged by size:
992 375 160
Bme1580I G_kGCm'C
Cuts at: 0 444 1527
Size: 444 1083
BmgBI CAC'GTC
Cuts at: 0 156 1527
Size: 156 1371
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 1008 1527
Size: 48 960 519
Fragments arranged by size:
960 519 48
BmtI G_CTAG'C
Cuts at: 0 352 1527
Size: 352 1175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 578 1527
Size: 437 141 949
Fragments arranged by size:
949 437 141
Bpu10I CC'TnA_GC
Cuts at: 0 353 1527
Size: 353 1174
BsaAI yAC'GTr
Cuts at: 0 798 1527
Size: 798 729
BsaWI w'CCGG_w
Cuts at: 0 1041 1527
Size: 1041 486
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1262 1527
Size: 1262 265
BseYI C'CCAG_C
Cuts at: 0 344 1527
Size: 344 1183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1527
Size: 82 1445
BsiHKAI G_wGCw'C
Cuts at: 0 378 1527
Size: 378 1149
BsmI GAATG_Cn'
Cuts at: 0 588 1527
Size: 588 939
BsmBI CGTCTCn'nnnn_
Cuts at: 0 97 1527
Size: 97 1430
Bsp1286I G_dGCh'C
Cuts at: 0 378 444 1527
Size: 378 66 1083
Fragments arranged by size:
1083 378 66
BspHI T'CATG_A
Cuts at: 0 941 1527
Size: 941 586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3 1527
Size: 3 1524
BsrDI GCAATG_nn'
Cuts at: 0 989 1527
Size: 989 538
BsrGI T'GTAC_A
Cuts at: 0 1157 1527
Size: 1157 370
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 1527
Size: 18 1509
BstYI r'GATC_y
Cuts at: 0 683 723 1182 1527
Size: 683 40 459 345
Fragments arranged by size:
683 459 345 40
Bsu36I CC'TnA_GG
Cuts at: 0 1077 1527
Size: 1077 450
BtgI C'CryG_G
Cuts at: 0 719 1527
Size: 719 808
DrdI GACnn_nn'nnGTC
Cuts at: 0 418 1527
Size: 418 1109
EaeI y'GGCC_r
Cuts at: 0 203 1527
Size: 203 1324
EarI CTCTTCn'nnn_
Cuts at: 0 149 1527
Size: 149 1378
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 1527
Size: 88 1439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 578 799 1527
Size: 437 141 221 728
Fragments arranged by size:
728 437 221 141
EcoO109I rG'GnC_Cy
Cuts at: 0 50 908 1527
Size: 50 858 619
Fragments arranged by size:
858 619 50
HincII GTy'rAC
Cuts at: 0 870 1166 1527
Size: 870 296 361
Fragments arranged by size:
870 361 296
HpaI GTT'AAC
Cuts at: 0 870 1527
Size: 870 657
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1278 1527
Size: 1278 249
MscI TGG'CCA
Cuts at: 0 205 1527
Size: 205 1322
MslI CAynn'nnrTG
Cuts at: 0 943 1527
Size: 943 584
MspA1I CmG'CkG
Cuts at: 0 307 861 1066 1527
Size: 307 554 205 461
Fragments arranged by size:
554 461 307 205
NdeI CA'TA_TG
Cuts at: 0 394 1527
Size: 394 1133
NheI G'CTAG_C
Cuts at: 0 348 1527
Size: 348 1179
NspI r_CATG'y
Cuts at: 0 1164 1294 1527
Size: 1164 130 233
Fragments arranged by size:
1164 233 130
PciI A'CATG_T
Cuts at: 0 1160 1290 1527
Size: 1160 130 237
Fragments arranged by size:
1160 237 130
PfoI T'CCnGG_A
Cuts at: 0 527 1527
Size: 527 1000
PpuMI rG'GwC_Cy
Cuts at: 0 50 908 1527
Size: 50 858 619
Fragments arranged by size:
858 619 50
PsiI TTA'TAA
Cuts at: 0 212 1527
Size: 212 1315
PstI C_TGCA'G
Cuts at: 0 113 435 1065 1496 1527
Size: 113 322 630 431 31
Fragments arranged by size:
630 431 322 113 31
PvuII CAG'CTG
Cuts at: 0 307 861 1066 1527
Size: 307 554 205 461
Fragments arranged by size:
554 461 307 205
ScaI AGT'ACT
Cuts at: 0 518 1527
Size: 518 1009
SexAI A'CCwGG_T
Cuts at: 0 1395 1527
Size: 1395 132
SfcI C'TryA_G
Cuts at: 0 109 431 1061 1492 1527
Size: 109 322 630 431 35
Fragments arranged by size:
630 431 322 109 35
SspI AAT'ATT
Cuts at: 0 1281 1527
Size: 1281 246
TatI w'GTAC_w
Cuts at: 0 516 765 1157 1527
Size: 516 249 392 370
Fragments arranged by size:
516 392 370 249
Tth111I GACn'n_nGTC
Cuts at: 0 157 1527
Size: 157 1370
XmnI GAAnn'nnTTC
Cuts at: 0 289 1256 1527
Size: 289 967 271
Fragments arranged by size:
967 289 271
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI AvaI BaeI BbsI
BciVI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BsaAI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrGI BstAPI BstYI Bsu36I BtgI
DrdI EaeI EarI EciI Eco57MI EcoO109I HincII HpaI
MmeI MscI MslI MspA1I NdeI NheI NspI PciI
PfoI PpuMI PsiI PstI PvuII ScaI SexAI SfcI
SspI TatI Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BclI
BfrBI BglI BglII BlpI BplI BpuEI BsaI BsaBI
BsaHI BsaXI BsiEI BsiWI BspEI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HindIII KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
SwaI TaqII TstI XbaI XcmI XhoI XmaI ZraI