Restriction map for
pFN21AB9552
(Linear) (Six-base) MAPSORT of: KIBB9552.seq Check: 7516 from: 1 to: 1398
KIBB9552.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 392 1398
Size: 392 1006
AlwNI CAG_nnn'CTG
Cuts at: 0 900 1398
Size: 900 498
ApaI G_GGCC'C
Cuts at: 0 98 1088 1097 1289 1340 1398
Size: 98 990 9 192 51 58
Fragments arranged by size:
990 192 98 58 51 9
AvaI C'yCGr_G
Cuts at: 0 72 135 682 1242 1398
Size: 72 63 547 560 156
Fragments arranged by size:
560 547 156 72 63
BanI G'GyrC_C
Cuts at: 0 261 286 644 914 1398
Size: 261 25 358 270 484
Fragments arranged by size:
484 358 270 261 25
BanII G_rGCy'C
Cuts at: 0 40 49 59 98 345 401 661 1088
Size: 40 9 10 39 247 56 260 427
Cuts at: 1088 1097 1289 1340 1398
Size: 9 192 51 58
Fragments arranged by size:
427 260 247 192 58 56 51 40
39 10 9 9
BbeI G_GCGC'C
Cuts at: 0 290 1398
Size: 290 1108
BbsI GAAGACnn'nnnn_
Cuts at: 0 742 1075 1398
Size: 742 333 323
Fragments arranged by size:
742 333 323
BbvCI CC'TCA_GC
Cuts at: 0 1147 1398
Size: 1147 251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 345 379 1398
Size: 345 34 1019
Fragments arranged by size:
1019 345 34
BglI GCCn_nnn'nGGC
Cuts at: 0 44 528 610 912 1092 1244 1398
Size: 44 484 82 302 180 152 154
Fragments arranged by size:
484 302 180 154 152 82 44
BlpI GC'TnA_GC
Cuts at: 0 34 238 395 400 470 1167 1398
Size: 34 204 157 5 70 697 231
Fragments arranged by size:
697 231 204 157 70 34 5
Bme1580I G_kGCm'C
Cuts at: 0 98 266 571 917 969 1088 1097 1289
Size: 98 168 305 346 52 119 9 192
Cuts at: 1289 1340 1398
Size: 51 58
Fragments arranged by size:
346 305 192 168 119 98 58 52
51 9
BmgBI CAC'GTC
Cuts at: 0 309 1398
Size: 309 1089
BmrI ACTGGGnnnn_n'
Cuts at: 0 1188 1335 1371 1398
Size: 1188 147 36 27
Fragments arranged by size:
1188 147 36 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 708 1398
Size: 708 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 1398
Size: 444 954
Bpu10I CC'TnA_GC
Cuts at: 0 344 1147 1398
Size: 344 803 251
Fragments arranged by size:
803 344 251
BsaI GGTCTCn'nnnn_
Cuts at: 0 862 1038 1069 1398
Size: 862 176 31 329
Fragments arranged by size:
862 329 176 31
BsaHI Gr'CG_yC
Cuts at: 0 287 1398
Size: 287 1111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 766 796 1398
Size: 766 30 602
Fragments arranged by size:
766 602 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 67 478 1398
Size: 22 45 411 920
Fragments arranged by size:
920 411 45 22
BseYI C'CCAG_C
Cuts at: 0 150 411 957 1324 1398
Size: 150 261 546 367 74
Fragments arranged by size:
546 367 261 150 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 1398
Size: 370 1028
BsiHKAI G_wGCw'C
Cuts at: 0 239 401 689 1398
Size: 239 162 288 709
Fragments arranged by size:
709 288 239 162
Bsp1286I G_dGCh'C
Cuts at: 0 40 49 59 98 239 266 345 401
Size: 40 9 10 39 141 27 79 56
Cuts at: 401 571 661 689 917 969 1088 1097 1289
Size: 170 90 28 228 52 119 9 192
Cuts at: 1289 1340 1398
Size: 51 58
Fragments arranged by size:
228 192 170 141 119 90 79 58
56 52 51 40 39 28 27 10
9 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 392 908 1398
Size: 392 516 490
Fragments arranged by size:
516 490 392
BsrBI CCG'CTC
Cuts at: 0 474 1398
Size: 474 924
BsrFI r'CCGG_y
Cuts at: 0 641 946 1398
Size: 641 305 452
Fragments arranged by size:
641 452 305
BssHII G'CGCG_C
Cuts at: 0 80 352 1398
Size: 80 272 1046
Fragments arranged by size:
1046 272 80
BssSI C'ACGA_G
Cuts at: 0 847 1398
Size: 847 551
BstXI CCAn_nnnn'nTGG
Cuts at: 0 706 1255 1398
Size: 706 549 143
Fragments arranged by size:
706 549 143
BstYI r'GATC_y
Cuts at: 0 15 998 1398
Size: 15 983 400
Fragments arranged by size:
983 400 15
Bsu36I CC'TnA_GG
Cuts at: 0 693 1221 1332 1398
Size: 693 528 111 66
Fragments arranged by size:
693 528 111 66
BtgI C'CryG_G
Cuts at: 0 41 705 785 974 1028 1079 1398
Size: 41 664 80 189 54 51 319
Fragments arranged by size:
664 319 189 80 54 51 41
BtsI GCAGTG_nn'
Cuts at: 0 969 1398
Size: 969 429
DraIII CAC_nnn'GTG
Cuts at: 0 1045 1398
Size: 1045 353
EaeI y'GGCC_r
Cuts at: 0 971 977 1236 1310 1398
Size: 971 6 259 74 88
Fragments arranged by size:
971 259 88 74 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 128 202 546 577 981 1398
Size: 19 109 74 344 31 404 417
Fragments arranged by size:
417 404 344 109 74 31 19
EcoICRI GAG'CTC
Cuts at: 0 399 1398
Size: 399 999
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 1398
Size: 444 954
EcoO109I rG'GnC_Cy
Cuts at: 0 94 1084 1085 1093 1094 1225 1285 1286
Size: 94 990 1 8 1 131 60 1
Cuts at: 1286 1336 1337 1398
Size: 50 1 61
Fragments arranged by size:
990 131 94 61 60 50 8 1
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 471 1398
Size: 471 927
HaeII r_GCGC'y
Cuts at: 0 290 453 629 783 1398
Size: 290 163 176 154 615
Fragments arranged by size:
615 290 176 163 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 733 765 766 798 841 873 1102 1134
Size: 733 32 1 32 43 32 229 32
Cuts at: 1134 1398
Size: 264
Fragments arranged by size:
733 264 229 43 32 32 32 32
1
HincII GTy'rAC
Cuts at: 0 282 1398
Size: 282 1116
KasI G'GCGC_C
Cuts at: 0 286 1398
Size: 286 1112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 748 869 884 1398
Size: 748 121 15 514
Fragments arranged by size:
748 514 121 15
MscI TGG'CCA
Cuts at: 0 979 1238 1398
Size: 979 259 160
Fragments arranged by size:
979 259 160
MslI CAynn'nnrTG
Cuts at: 0 271 1398
Size: 271 1127
MspA1I CmG'CkG
Cuts at: 0 62 242 1081 1296 1398
Size: 62 180 839 215 102
Fragments arranged by size:
839 215 180 102 62
NaeI GCC'GGC
Cuts at: 0 948 1398
Size: 948 450
NarI GG'CG_CC
Cuts at: 0 287 1398
Size: 287 1111
NcoI C'CATG_G
Cuts at: 0 705 785 1398
Size: 705 80 613
Fragments arranged by size:
705 613 80
NgoMIV G'CCGG_C
Cuts at: 0 946 1398
Size: 946 452
PasI CC'CwG_GG
Cuts at: 0 552 1089 1398
Size: 552 537 309
Fragments arranged by size:
552 537 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 1361 1398
Size: 1361 37
PpuMI rG'GwC_Cy
Cuts at: 0 1225 1398
Size: 1225 173
PspOMI G'GGCC_C
Cuts at: 0 94 1084 1093 1285 1336 1398
Size: 94 990 9 192 51 62
Fragments arranged by size:
990 192 94 62 51 9
PstI C_TGCA'G
Cuts at: 0 749 1398
Size: 749 649
SacI G_AGCT'C
Cuts at: 0 401 1398
Size: 401 997
SacII CC_GC'GG
Cuts at: 0 1082 1398
Size: 1082 316
SexAI A'CCwGG_T
Cuts at: 0 1041 1398
Size: 1041 357
SfcI C'TryA_G
Cuts at: 0 745 1398
Size: 745 653
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1092 1244 1398
Size: 1092 152 154
Fragments arranged by size:
1092 154 152
SfoI GGC'GCC
Cuts at: 0 288 1398
Size: 288 1110
SmaI CCC'GGG
Cuts at: 0 74 137 1398
Size: 74 63 1261
Fragments arranged by size:
1261 74 63
StuI AGG'CCT
Cuts at: 0 1252 1398
Size: 1252 146
StyI C'CwwG_G
Cuts at: 0 705 785 1398
Size: 705 80 613
Fragments arranged by size:
705 613 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 662 729 932 1398
Size: 662 67 203 466
Fragments arranged by size:
662 466 203 67
Tth111I GACn'n_nGTC
Cuts at: 0 1386 1398
Size: 1386 12
XmaI C'CCGG_G
Cuts at: 0 72 135 1398
Size: 72 63 1263
Fragments arranged by size:
1263 72 63
XmnI GAAnn'nnTTC
Cuts at: 0 328 1398
Size: 328 1070
Enzymes that do cut:
AarI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EciI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PpuMI PspOMI PstI SacI SacII SexAI SfcI
SfiI SfoI SmaI StuI StyI TaqII Tth111I XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BglII BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI
EarI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI
SanDI SapI SbfI ScaI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI XbaI
XcmI XhoI ZraI