(Circular) (Six-base) MAPSORT of: pFN21AB6320.seq Check: 2959 from: 1 to: 5551 pFN21AB6320 5551 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3361 278 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2894 1937 Size: 957 4594 Acc65I G'GTAC_C Cuts at: 2722 2872 2722 Size: 150 5401 AclI AA'CG_TT Cuts at: 1563 3678 4051 1563 Size: 2115 373 3063 Fragments arranged by size: 3063 2115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 3614 4998 5266 1632 Size: 539 1443 1384 268 1917 Fragments arranged by size: 1917 1443 1384 539 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 4450 5427 1203 Size: 3247 977 1327 Fragments arranged by size: 3247 1327 977 AgeI A'CCGG_T Cuts at: 4382 4382 Size: 5551 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 2306 2399 1166 Size: 1140 93 4318 Fragments arranged by size: 4318 1140 93 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5551 AlwNI CAG_nnn'CTG Cuts at: 2125 3294 4866 2125 Size: 1169 1572 2810 Fragments arranged by size: 2810 1572 1169 ApaLI G'TGCA_C Cuts at: 3608 4764 3608 Size: 1156 4395 ApoI r'AATT_y Cuts at: 1493 2860 3086 3238 5347 1493 Size: 1367 226 152 2109 1697 Fragments arranged by size: 2109 1697 1367 226 152 AseI AT'TA_AT Cuts at: 160 1032 4105 160 Size: 872 3073 1606 Fragments arranged by size: 3073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2428 2726 2876 1870 Size: 73 72 413 298 150 4545 Fragments arranged by size: 4545 413 298 150 73 72 BamHI G'GATC_C Cuts at: 2881 3248 2881 Size: 367 5184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 2452 2722 2872 4327 Size: 325 217 627 513 152 270 150 1455 Cuts at: 4327 618 Size: 1842 Fragments arranged by size: 1842 1455 627 513 325 270 217 152 150 BanII G_rGCy'C Cuts at: 1391 2670 2870 1391 Size: 1279 200 4072 Fragments arranged by size: 4072 1279 200 BbeI G_GCGC'C Cuts at: 2456 2456 Size: 5551 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4580 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3742 3776 1903 Size: 34 1805 34 3678 Fragments arranged by size: 3678 1805 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2264 3443 4653 2264 Size: 1179 1210 3162 Fragments arranged by size: 3162 1210 1179 BclI T'GATC_A Cuts at: 1548 1548 Size: 5551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4162 136 Size: 107 122 71 631 605 2490 1525 Fragments arranged by size: 2490 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2832 5546 2832 Size: 2714 2837 BlpI GC'TnA_GC Cuts at: 2968 2968 Size: 5551 Bme1580I G_kGCm'C Cuts at: 1790 3612 4768 1790 Size: 1822 1156 2573 Fragments arranged by size: 2573 1822 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5551 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2104 3282 4240 Size: 416 53 83 399 24 687 1178 958 Cuts at: 4240 442 Size: 1753 Fragments arranged by size: 1753 1178 958 687 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4211 1239 Size: 2972 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3676 4541 4839 3676 Size: 865 298 4388 Fragments arranged by size: 4388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2012 2348 2651 4214 882 Size: 617 513 336 303 1563 2219 Fragments arranged by size: 2219 1563 617 513 336 303 BsaAI yAC'GTr Cuts at: 493 1204 3342 493 Size: 711 2138 2702 Fragments arranged by size: 2702 2138 711 BsaBI GATnn'nnATC Cuts at: 5545 5545 Size: 5551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2453 3358 3740 4375 Size: 53 83 186 936 920 905 382 635 Cuts at: 4375 275 Size: 1451 Fragments arranged by size: 1451 936 920 905 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3984 4382 4656 4803 5214 3984 Size: 398 274 147 411 4321 Fragments arranged by size: 4321 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5326 Fragments arranged by size: 5326 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 1186 Size: 1483 4068 BseYI C'CCAG_C Cuts at: 2598 4754 5250 2598 Size: 2156 496 2899 Fragments arranged by size: 2899 2156 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4978 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3762 4790 1540 Size: 275 186 1000 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2670 3612 3697 4768 2670 Size: 942 85 1071 3453 Fragments arranged by size: 3453 1071 942 85 BsmI GAATG_Cn' Cuts at: 3062 3155 3062 Size: 93 5458 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2670 2870 3612 3697 4768 1391 Size: 399 880 200 742 85 1071 2174 Fragments arranged by size: 2174 1071 880 742 399 200 85 BspEI T'CCGG_A Cuts at: 5214 5214 Size: 5551 BspHI T'CATG_A Cuts at: 3440 5170 3440 Size: 1730 3821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2655 2906 844 Size: 1811 251 3489 Fragments arranged by size: 3489 1811 251 BsrBI CCG'CTC Cuts at: 1394 1823 2314 3438 1394 Size: 429 491 1124 3507 Fragments arranged by size: 3507 1124 491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2283 4046 4220 65 Size: 1183 1035 1763 174 1396 Fragments arranged by size: 1763 1396 1183 1035 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4195 4382 1700 Size: 110 2385 187 2869 Fragments arranged by size: 2869 2385 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5551 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5551 BssSI C'ACGA_G Cuts at: 3611 4623 3611 Size: 1012 4539 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 2575 794 Size: 1679 102 3770 Fragments arranged by size: 3770 1679 102 BstBI TT'CG_AA Cuts at: 4355 4355 Size: 5551 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5551 BstXI CCAn_nnnn'nTGG Cuts at: 2401 2401 Size: 5551 BstYI r'GATC_y Cuts at: 1972 2121 2541 2832 2881 3248 3633 3650 5102 Size: 149 420 291 49 367 385 17 1452 Cuts at: 5102 5188 5200 5546 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 420 385 367 346 291 149 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2400 513 Size: 552 939 396 3664 Fragments arranged by size: 3664 939 552 396 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2725 2802 487 Size: 683 56 1499 77 3236 Fragments arranged by size: 3236 1499 683 77 56 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2855 2855 Size: 5551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4558 809 Size: 507 3242 1802 Fragments arranged by size: 3242 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2361 2536 Size: 54 1425 50 275 18 286 245 175 Cuts at: 2536 2998 3887 5516 8 Size: 462 889 1629 43 Fragments arranged by size: 1629 1425 889 462 286 275 245 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 1537 Size: 275 1186 4090 Fragments arranged by size: 4090 1186 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2327 3481 1353 Size: 974 1154 3423 Fragments arranged by size: 3423 1154 974 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4138 4512 4658 387 Size: 1064 138 2549 374 146 1280 Fragments arranged by size: 2549 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2668 2668 Size: 5551 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 3614 4211 4998 5266 1239 Size: 393 539 1443 597 787 268 1524 Fragments arranged by size: 1524 1443 787 597 539 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2651 Size: 5551 EcoO109I rG'GnC_Cy Cuts at: 2420 2420 Size: 5551 EcoRI G'AATT_C Cuts at: 2860 3238 2860 Size: 378 5173 EcoRV GAT'ATC Cuts at: 2131 2281 2131 Size: 150 5401 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2854 2854 Size: 5551 FspI TGC'GCA Cuts at: 4057 4057 Size: 5551 HaeII r_GCGC'y Cuts at: 1678 2199 2456 4698 1678 Size: 521 257 2242 2531 Fragments arranged by size: 2531 2242 521 257 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4192 4224 4266 4298 1373 Size: 32 135 32 2620 32 42 32 2626 Fragments arranged by size: 2626 2620 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2895 3141 669 Size: 988 281 957 246 3079 Fragments arranged by size: 3079 988 957 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5551 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KasI G'GCGC_C Cuts at: 2452 2452 Size: 5551 KpnI G_GTAC'C Cuts at: 2726 2876 2726 Size: 150 5401 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4665 4849 1305 Size: 3360 184 2007 Fragments arranged by size: 3360 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2118 2538 10 Size: 54 1425 343 286 420 3023 Fragments arranged by size: 3023 1425 420 343 286 54 MslI CAynn'nnrTG Cuts at: 518 1166 2306 2399 3509 3868 4027 518 Size: 648 1140 93 1110 359 159 2042 Fragments arranged by size: 2042 1140 1110 648 359 159 93 MspA1I CmG'CkG Cuts at: 1606 2967 3644 4792 5037 1606 Size: 1361 677 1148 245 2120 Fragments arranged by size: 2120 1361 1148 677 245 NarI GG'CG_CC Cuts at: 2453 2453 Size: 5551 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4060 Fragments arranged by size: 4060 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NruI TCG'CGA Cuts at: 2787 2787 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2437 2476 2497 2909 4454 1132 Size: 97 1208 39 21 412 1545 2229 Fragments arranged by size: 2229 1545 1208 412 97 39 21 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5551 PciI A'CATG_T Cuts at: 4450 4450 Size: 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 2125 1108 Size: 1017 4534 PfoI T'CCnGG_A Cuts at: 2124 2124 Size: 5551 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3620 3652 5133 5165 3620 Size: 32 1481 32 4006 Fragments arranged by size: 4006 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5551 PsiI TTA'TAA Cuts at: 3121 3121 Size: 5551 PstI C_TGCA'G Cuts at: 830 2799 2903 830 Size: 1969 104 3478 Fragments arranged by size: 3478 1969 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 SacI G_AGCT'C Cuts at: 2670 2670 Size: 5551 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SbfI CC_TGCA'GG Cuts at: 2799 2903 2799 Size: 104 5447 ScaI AGT'ACT Cuts at: 3799 5511 3799 Size: 1712 3839 SfcI C'TryA_G Cuts at: 826 1045 2795 2899 4034 4715 4906 826 Size: 219 1750 104 1135 681 191 1471 Fragments arranged by size: 1750 1471 1135 681 219 191 104 SfoI GGC'GCC Cuts at: 2454 2454 Size: 5551 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5551 SmaI CCC'GGG Cuts at: 1872 2430 2728 2878 1872 Size: 558 298 150 4545 Fragments arranged by size: 4545 558 298 150 SmlI C'TyrA_G Cuts at: 820 1017 1943 3655 4556 4818 820 Size: 197 926 1712 901 262 1553 Fragments arranged by size: 1712 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2437 2909 1132 Size: 1305 472 3774 Fragments arranged by size: 3774 1305 472 SspI AAT'ATT Cuts at: 5 52 3475 5 Size: 47 3423 2081 Fragments arranged by size: 3423 2081 47 StuI AGG'CCT Cuts at: 1517 2168 2526 1517 Size: 651 358 4542 Fragments arranged by size: 4542 651 358 StyI C'CwwG_G Cuts at: 513 1065 2004 2990 513 Size: 552 939 986 3074 Fragments arranged by size: 3074 986 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 3578 3595 3748 3933 4378 325 Size: 1255 705 1293 17 153 185 445 1498 Fragments arranged by size: 1498 1293 1255 705 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3797 5509 96 Size: 274 80 33 51 1443 1820 1712 138 Fragments arranged by size: 1820 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5511 5543 5511 Size: 32 5519 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2401 Size: 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5551 XmaI C'CCGG_G Cuts at: 1870 2428 2726 2876 1870 Size: 558 298 150 4545 Fragments arranged by size: 4545 558 298 150 XmnI GAAnn'nnTTC Cuts at: 3680 3680 Size: 5551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3359 276 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI