Restriction map for pFN21AB6320
 (Circular) (Six-base) MAPSORT of: pFN21AB6320.seq  Check: 2959  from: 1  to: 5551



pFN21AB6320  5551 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:38  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3361     278 
   Size:         53      83     186     936    1825    2468

  Fragments arranged by size: 

                2468    1825     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2894    1937 
   Size:        957    4594
 
Acc65I G'GTAC_C

Cuts at:   2722    2872    2722 
   Size:        150    5401
 
AclI AA'CG_TT

Cuts at:   1563    3678    4051    1563 
   Size:       2115     373    3063

  Fragments arranged by size: 

                3063    2115     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2171    3614    4998    5266    1632 
   Size:        539    1443    1384     268    1917

  Fragments arranged by size: 

                1917    1443    1384     539     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5354
 
AflIII A'CryG_T

Cuts at:   1203    4450    5427    1203 
   Size:       3247     977    1327

  Fragments arranged by size: 

                3247    1327     977
 
AgeI A'CCGG_T

Cuts at:   4382    4382 
   Size:       5551
 
AhdI GACnn_n'nnGTC

Cuts at:   4280    4280 
   Size:       5551
 
AleI CACnn'nnGTG

Cuts at:   1166    2306    2399    1166 
   Size:       1140      93    4318

  Fragments arranged by size: 

                4318    1140      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5551
 
AlwNI CAG_nnn'CTG

Cuts at:   2125    3294    4866    2125 
   Size:       1169    1572    2810

  Fragments arranged by size: 

                2810    1572    1169
 
ApaLI G'TGCA_C

Cuts at:   3608    4764    3608 
   Size:       1156    4395
 
ApoI r'AATT_y

Cuts at:   1493    2860    3086    3238    5347    1493 
   Size:       1367     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1367     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4105     160 
   Size:        872    3073    1606

  Fragments arranged by size: 

                3073    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5551
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2015    2428    2726    2876    1870 
   Size:         73      72     413     298     150    4545

  Fragments arranged by size: 

                4545     413     298     150      73      72
 
BamHI G'GATC_C

Cuts at:   2881    3248    2881 
   Size:        367    5184
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2300    2452    2722    2872    4327 
   Size:        325     217     627     513     152     270     150    1455

Cuts at:   4327     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455     627     513     325     270     217     152
                 150
 
BanII G_rGCy'C

Cuts at:   1391    2670    2870    1391 
   Size:       1279     200    4072

  Fragments arranged by size: 

                4072    1279     200
 
BbeI G_GCGC'C

Cuts at:   2456    2456 
   Size:       5551
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4580
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3742    3776    1903 
   Size:         34    1805      34    3678

  Fragments arranged by size: 

                3678    1805      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2264    3443    4653    2264 
   Size:       1179    1210    3162

  Fragments arranged by size: 

                3162    1210    1179
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5551
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4162     136 
   Size:        107     122      71     631     605    2490    1525

  Fragments arranged by size: 

                2490    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2832    5546    2832 
   Size:       2714    2837
 
BlpI GC'TnA_GC

Cuts at:   2968    2968 
   Size:       5551
 
Bme1580I G_kGCm'C

Cuts at:   1790    3612    4768    1790 
   Size:       1822    1156    2573

  Fragments arranged by size: 

                2573    1822    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5551
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2104    3282    4240 
   Size:        416      53      83     399      24     687    1178     958

Cuts at:   4240     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1178     958     687     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5551
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4211    1239 
   Size:       2972    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3676    4541    4839    3676 
   Size:        865     298    4388

  Fragments arranged by size: 

                4388     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2012    2348    2651    4214     882 
   Size:        617     513     336     303    1563    2219

  Fragments arranged by size: 

                2219    1563     617     513     336     303
 
BsaAI yAC'GTr

Cuts at:    493    1204    3342     493 
   Size:        711    2138    2702

  Fragments arranged by size: 

                2702    2138     711
 
BsaBI GATnn'nnATC

Cuts at:   5545    5545 
   Size:       5551
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2453    3358    3740    4375 
   Size:         53      83     186     936     920     905     382     635

Cuts at:   4375     275 
   Size:       1451

  Fragments arranged by size: 

                1451     936     920     905     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3984    4382    4656    4803    5214    3984 
   Size:        398     274     147     411    4321

  Fragments arranged by size: 

                4321     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5326

  Fragments arranged by size: 

                5326     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2669    1186 
   Size:       1483    4068
 
BseYI C'CCAG_C

Cuts at:   2598    4754    5250    2598 
   Size:       2156     496    2899

  Fragments arranged by size: 

                2899    2156     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4978
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3001    3762    4790    1540 
   Size:        275     186    1000     761    1028    2301

  Fragments arranged by size: 

                2301    1028    1000     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2670    3612    3697    4768    2670 
   Size:        942      85    1071    3453

  Fragments arranged by size: 

                3453    1071     942      85
 
BsmI GAATG_Cn'

Cuts at:   3062    3155    3062 
   Size:         93    5458
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2670    2870    3612    3697    4768    1391 
   Size:        399     880     200     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     880     742     399     200      85
 
BspEI T'CCGG_A

Cuts at:   5214    5214 
   Size:       5551
 
BspHI T'CATG_A

Cuts at:   3440    5170    3440 
   Size:       1730    3821
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2655    2906     844 
   Size:       1811     251    3489

  Fragments arranged by size: 

                3489    1811     251
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2314    3438    1394 
   Size:        429     491    1124    3507

  Fragments arranged by size: 

                3507    1124     491     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2283    4046    4220      65 
   Size:       1183    1035    1763     174    1396

  Fragments arranged by size: 

                1763    1396    1183    1035     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4195    4382    1700 
   Size:        110    2385     187    2869

  Fragments arranged by size: 

                2869    2385     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5551
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5551
 
BssSI C'ACGA_G

Cuts at:   3611    4623    3611 
   Size:       1012    4539
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2473    2575     794 
   Size:       1679     102    3770

  Fragments arranged by size: 

                3770    1679     102
 
BstBI TT'CG_AA

Cuts at:   4355    4355 
   Size:       5551
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5551
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2401    2401 
   Size:       5551
 
BstYI r'GATC_y

Cuts at:   1972    2121    2541    2832    2881    3248    3633    3650    5102 
   Size:        149     420     291      49     367     385      17    1452

Cuts at:   5102    5188    5200    5546    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     420     385     367     346     291     149
                  86      49      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2400     513 
   Size:        552     939     396    3664

  Fragments arranged by size: 

                3664     939     552     396
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2725    2802     487 
   Size:        683      56    1499      77    3236

  Fragments arranged by size: 

                3236    1499     683      77      56
 
BtsI GCAGTG_nn'

Cuts at:   3067    3861    3881    3067 
   Size:        794      20    4737

  Fragments arranged by size: 

                4737     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5551
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5516
 
DraI TTT'AAA

Cuts at:   2855    2855 
   Size:       5551
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4558     809 
   Size:        507    3242    1802

  Fragments arranged by size: 

                3242    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2116    2361    2536 
   Size:         54    1425      50     275      18     286     245     175

Cuts at:   2536    2998    3887    5516       8 
   Size:        462     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     462     286     275     245     175
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2998    1537 
   Size:        275    1186    4090

  Fragments arranged by size: 

                4090    1186     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2327    3481    1353 
   Size:        974    1154    3423

  Fragments arranged by size: 

                3423    1154     974
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4138    4512    4658     387 
   Size:       1064     138    2549     374     146    1280

  Fragments arranged by size: 

                2549    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2668    2668 
   Size:       5551
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2171    3614    4211    4998    5266    1239 
   Size:        393     539    1443     597     787     268    1524

  Fragments arranged by size: 

                1524    1443     787     597     539     393     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2651    2651 
   Size:       5551
 
EcoO109I rG'GnC_Cy

Cuts at:   2420    2420 
   Size:       5551
 
EcoRI G'AATT_C

Cuts at:   2860    3238    2860 
   Size:        378    5173
 
EcoRV GAT'ATC

Cuts at:   2131    2281    2131 
   Size:        150    5401
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2854    2854 
   Size:       5551
 
FspI TGC'GCA

Cuts at:   4057    4057 
   Size:       5551
 
HaeII r_GCGC'y

Cuts at:   1678    2199    2456    4698    1678 
   Size:        521     257    2242    2531

  Fragments arranged by size: 

                2531    2242     521     257
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4192    4224    4266    4298    1373 
   Size:         32     135      32    2620      32      42      32    2626

  Fragments arranged by size: 

                2626    2620     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2895    3141     669 
   Size:        988     281     957     246    3079

  Fragments arranged by size: 

                3079     988     957     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5551
 
HpaI GTT'AAC

Cuts at:   3141    3141 
   Size:       5551
 
KasI G'GCGC_C

Cuts at:   2452    2452 
   Size:       5551
 
KpnI G_GTAC'C

Cuts at:   2726    2876    2726 
   Size:        150    5401
 
MfeI C'AATT_G

Cuts at:   3150    3150 
   Size:       5551
 
MluI A'CGCG_T

Cuts at:   5427    5427 
   Size:       5551
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4665    4849    1305 
   Size:       3360     184    2007

  Fragments arranged by size: 

                3360    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2118    2538      10 
   Size:         54    1425     343     286     420    3023

  Fragments arranged by size: 

                3023    1425     420     343     286      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2306    2399    3509    3868    4027     518 
   Size:        648    1140      93    1110     359     159    2042

  Fragments arranged by size: 

                2042    1140    1110     648     359     159      93
 
MspA1I CmG'CkG

Cuts at:   1606    2967    3644    4792    5037    1606 
   Size:       1361     677    1148     245    2120

  Fragments arranged by size: 

                2120    1361    1148     677     245
 
NarI GG'CG_CC

Cuts at:   2453    2453 
   Size:       5551
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4060

  Fragments arranged by size: 

                4060     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5551
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5551
 
NotI GC'GGCC_GC

Cuts at:   2998    2998 
   Size:       5551
 
NruI TCG'CGA

Cuts at:   2787    2787 
   Size:       5551
 
NspI r_CATG'y

Cuts at:   1132    1229    2437    2476    2497    2909    4454    1132 
   Size:         97    1208      39      21     412    1545    2229

  Fragments arranged by size: 

                2229    1545    1208     412      97      39      21
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5551
 
PciI A'CATG_T

Cuts at:   4450    4450 
   Size:       5551
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2125    1108 
   Size:       1017    4534
 
PfoI T'CCnGG_A

Cuts at:   2124    2124 
   Size:       5551
 
PmeI GTTT'AAAC

Cuts at:   2855    2855 
   Size:       5551
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3620    3652    5133    5165    3620 
   Size:         32    1481      32    4006

  Fragments arranged by size: 

                4006    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5551
 
PsiI TTA'TAA

Cuts at:   3121    3121 
   Size:       5551
 
PstI C_TGCA'G

Cuts at:    830    2799    2903     830 
   Size:       1969     104    3478

  Fragments arranged by size: 

                3478    1969     104
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5551
 
SacI G_AGCT'C

Cuts at:   2670    2670 
   Size:       5551
 
SalI G'TCGA_C

Cuts at:   1936    2893    1936 
   Size:        957    4594
 
SbfI CC_TGCA'GG

Cuts at:   2799    2903    2799 
   Size:        104    5447
 
ScaI AGT'ACT

Cuts at:   3799    5511    3799 
   Size:       1712    3839
 
SfcI C'TryA_G

Cuts at:    826    1045    2795    2899    4034    4715    4906     826 
   Size:        219    1750     104    1135     681     191    1471

  Fragments arranged by size: 

                1750    1471    1135     681     219     191     104
 
SfoI GGC'GCC

Cuts at:   2454    2454 
   Size:       5551
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5551
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5551
 
SmaI CCC'GGG

Cuts at:   1872    2430    2728    2878    1872 
   Size:        558     298     150    4545

  Fragments arranged by size: 

                4545     558     298     150
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3655    4556    4818     820 
   Size:        197     926    1712     901     262    1553

  Fragments arranged by size: 

                1712    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3342     493 
   Size:       2849    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5551
 
SphI G_CATG'C

Cuts at:   1132    2437    2909    1132 
   Size:       1305     472    3774

  Fragments arranged by size: 

                3774    1305     472
 
SspI AAT'ATT

Cuts at:      5      52    3475       5 
   Size:         47    3423    2081

  Fragments arranged by size: 

                3423    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2168    2526    1517 
   Size:        651     358    4542

  Fragments arranged by size: 

                4542     651     358
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2990     513 
   Size:        552     939     986    3074

  Fragments arranged by size: 

                3074     986     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2285    3578    3595    3748    3933    4378     325 
   Size:       1255     705    1293      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1293    1255     705     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3797    5509      96 
   Size:        274      80      33      51    1443    1820    1712     138

  Fragments arranged by size: 

                1820    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5511    5543    5511 
   Size:         32    5519
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5551
 
XbaI T'CTAG_A

Cuts at:   2887    2887 
   Size:       5551
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2401    2401 
   Size:       5551
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5551
 
XmaI C'CCGG_G

Cuts at:   1870    2428    2726    2876    1870 
   Size:        558     298     150    4545

  Fragments arranged by size: 

                4545     558     298     150
 
XmnI GAAnn'nnTTC

Cuts at:   3680    3680 
   Size:       5551
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3359     276 
   Size:         53      83     186     936    1825    2468

  Fragments arranged by size: 

                2468    1825     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NotI     NruI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SacI     SalI
     SbfI     ScaI     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII
     FseI    FspAI     NaeI   NgoMIV     NsiI     PacI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    PvuII    RsrII    SacII    SanDI     SapI
    SexAI     SfiI     SrfI     SwaI
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