(Circular) (Six-base) MAPSORT of: pFN21AB7706.seq Check: 5660 from: 1 to: 6100 pFN21AB7706 6100 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2012 3910 278 Size: 53 83 186 936 476 1898 2468 Fragments arranged by size: 2468 1898 936 476 186 83 53 AccI GT'mk_AC Cuts at: 1937 2829 3443 1937 Size: 892 614 4594 Fragments arranged by size: 4594 892 614 Acc65I G'GTAC_C Cuts at: 3421 3421 Size: 6100 AclI AA'CG_TT Cuts at: 1563 4227 4600 1563 Size: 2664 373 3063 Fragments arranged by size: 3063 2664 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3320 4163 5547 5815 1632 Size: 1688 843 1384 268 1917 Fragments arranged by size: 1917 1688 1384 843 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5903 AflIII A'CryG_T Cuts at: 1203 4999 5976 1203 Size: 3796 977 1327 Fragments arranged by size: 3796 1327 977 AgeI A'CCGG_T Cuts at: 4931 4931 Size: 6100 AhdI GACnn_n'nnGTC Cuts at: 4829 4829 Size: 6100 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6100 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6100 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2988 3020 2988 Size: 32 6068 AlwNI CAG_nnn'CTG Cuts at: 3135 3178 3343 3843 5415 3135 Size: 43 165 500 1572 3820 Fragments arranged by size: 3820 1572 500 165 43 ApaLI G'TGCA_C Cuts at: 4157 5313 4157 Size: 1156 4944 ApoI r'AATT_y Cuts at: 1493 2336 2441 3147 3409 3635 3787 5896 1493 Size: 843 105 706 262 226 152 2109 1697 Fragments arranged by size: 2109 1697 843 706 262 226 152 105 AseI AT'TA_AT Cuts at: 160 1032 4654 160 Size: 872 3622 1606 Fragments arranged by size: 3622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 AvaI C'yCGr_G Cuts at: 1870 1943 3425 1870 Size: 73 1482 4545 Fragments arranged by size: 4545 1482 73 BamHI G'GATC_C Cuts at: 3430 3797 3430 Size: 367 5733 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 3421 4876 618 Size: 325 217 627 357 1277 1455 1842 Fragments arranged by size: 1842 1455 1277 627 357 325 217 BanII G_rGCy'C Cuts at: 1391 3222 3371 3419 1391 Size: 1831 149 48 4072 Fragments arranged by size: 4072 1831 149 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5129 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4291 4325 1903 Size: 34 2354 34 3678 Fragments arranged by size: 3678 2354 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2722 3992 5202 2722 Size: 1270 1210 3620 Fragments arranged by size: 3620 1270 1210 BclI T'GATC_A Cuts at: 1548 2695 1548 Size: 1147 4953 BfrBI ATG'CAT Cuts at: 2870 2870 Size: 6100 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4711 136 Size: 107 122 71 631 605 3039 1525 Fragments arranged by size: 3039 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2213 6095 2213 Size: 3882 2218 BlpI GC'TnA_GC Cuts at: 3171 3517 3171 Size: 346 5754 Bme1580I G_kGCm'C Cuts at: 1790 4161 5317 1790 Size: 2371 1156 2573 Fragments arranged by size: 2573 2371 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6100 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2757 3831 4789 Size: 416 53 83 399 24 1340 1074 958 Cuts at: 4789 442 Size: 1753 Fragments arranged by size: 1753 1340 1074 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2601 1056 Size: 1545 4555 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2400 4760 1239 Size: 941 220 2360 2579 Fragments arranged by size: 2579 2360 941 220 Bpu10I CC'TnA_GC Cuts at: 2483 2483 Size: 6100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2191 4225 5090 5388 2191 Size: 2034 865 298 2903 Fragments arranged by size: 2903 2034 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4763 882 Size: 617 3264 2219 Fragments arranged by size: 3264 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3891 493 Size: 711 2687 2702 Fragments arranged by size: 2702 2687 711 BsaBI GATnn'nnATC Cuts at: 6094 6094 Size: 6100 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2227 3907 4289 Size: 53 83 186 936 476 218 1680 382 Cuts at: 4289 4924 275 Size: 635 1451 Fragments arranged by size: 1680 1451 936 635 476 382 218 186 83 53 BsaWI w'CCGG_w Cuts at: 4533 4931 5205 5352 5763 4533 Size: 398 274 147 411 4870 Fragments arranged by size: 4870 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2656 2686 1178 Size: 30 165 30 1253 30 4592 Fragments arranged by size: 4592 1253 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2099 2661 1186 Size: 910 3 562 4625 Fragments arranged by size: 4625 910 562 3 BseYI C'CCAG_C Cuts at: 2620 2656 2718 3128 5303 5799 2620 Size: 36 62 410 2175 496 2921 Fragments arranged by size: 2921 2175 496 410 62 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5527 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3550 4311 5339 1540 Size: 275 186 1549 761 1028 2301 Fragments arranged by size: 2301 1549 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3222 4161 4246 5317 3222 Size: 939 85 1071 4005 Fragments arranged by size: 4005 1071 939 85 BsmI GAATG_Cn' Cuts at: 3047 3611 3704 3047 Size: 564 93 5443 Fragments arranged by size: 5443 564 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3222 3371 3419 4161 4246 5317 1391 Size: 399 1432 149 48 742 85 1071 2174 Fragments arranged by size: 2174 1432 1071 742 399 149 85 48 BspEI T'CCGG_A Cuts at: 5763 5763 Size: 6100 BspHI T'CATG_A Cuts at: 2308 3989 5719 2308 Size: 1681 1730 2689 Fragments arranged by size: 2689 1730 1681 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2224 3455 844 Size: 1380 1231 3489 Fragments arranged by size: 3489 1380 1231 BsrBI CCG'CTC Cuts at: 1394 1823 3987 1394 Size: 429 2164 3507 Fragments arranged by size: 3507 2164 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4595 4769 65 Size: 1183 3347 174 1396 Fragments arranged by size: 3347 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3279 4744 4931 1700 Size: 110 1469 1465 187 2869 Fragments arranged by size: 2869 1469 1465 187 110 BsrGI T'GTAC_A Cuts at: 96 3067 96 Size: 2971 3129 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6100 BssSI C'ACGA_G Cuts at: 4160 5172 4160 Size: 1012 5088 BstAPI GCAn_nnn'nTGC Cuts at: 794 2111 794 Size: 1317 4783 BstBI TT'CG_AA Cuts at: 4904 4904 Size: 6100 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6100 BstYI r'GATC_y Cuts at: 1972 2213 2348 2905 3430 3797 4182 4199 5651 Size: 241 135 557 525 367 385 17 1452 Cuts at: 5651 5737 5749 6095 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 557 525 385 367 346 241 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2148 3196 513 Size: 552 1083 1048 3417 Fragments arranged by size: 3417 1083 1048 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5361 Fragments arranged by size: 5361 683 56 BtsI GCAGTG_nn' Cuts at: 3616 4410 4430 3616 Size: 794 20 5286 Fragments arranged by size: 5286 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6100 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6065 DraI TTT'AAA Cuts at: 3404 3404 Size: 6100 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2640 5107 809 Size: 507 1324 2467 1802 Fragments arranged by size: 2467 1802 1324 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2288 3547 Size: 54 1425 50 275 18 186 272 1259 Cuts at: 3547 4436 6065 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1259 889 275 272 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3547 1537 Size: 275 1735 4090 Fragments arranged by size: 4090 1735 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2796 3339 4030 1353 Size: 1443 543 691 3423 Fragments arranged by size: 3423 1443 691 543 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4687 5061 5207 387 Size: 1064 138 3098 374 146 1280 Fragments arranged by size: 3098 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3220 3220 Size: 6100 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2400 3320 4163 4760 5547 5815 Size: 393 548 220 920 843 597 787 268 Cuts at: 5815 1239 Size: 1524 Fragments arranged by size: 1524 920 843 787 597 548 393 268 220 EcoRI G'AATT_C Cuts at: 2441 3409 3787 2441 Size: 968 378 4754 Fragments arranged by size: 4754 968 378 FspI TGC'GCA Cuts at: 4606 4606 Size: 6100 HaeII r_GCGC'y Cuts at: 1678 2287 5247 1678 Size: 609 2960 2531 Fragments arranged by size: 2960 2531 609 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4741 4773 4815 4847 1373 Size: 32 135 32 3169 32 42 32 2626 Fragments arranged by size: 3169 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3193 3444 3690 669 Size: 988 281 1255 251 246 3079 Fragments arranged by size: 3079 1255 988 281 251 246 HindIII A'AGCT_T Cuts at: 748 2859 748 Size: 2111 3989 HpaI GTT'AAC Cuts at: 3690 3690 Size: 6100 KpnI G_GTAC'C Cuts at: 3425 3425 Size: 6100 MfeI C'AATT_G Cuts at: 2970 3699 2970 Size: 729 5371 MluI A'CGCG_T Cuts at: 5976 5976 Size: 6100 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2304 2833 3287 5214 5398 1305 Size: 999 529 454 1927 184 2007 Fragments arranged by size: 2007 1927 999 529 454 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2018 10 Size: 54 1425 343 186 4092 Fragments arranged by size: 4092 1425 343 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 3227 3242 3324 4058 4417 4576 518 Size: 648 2061 15 82 734 359 159 2042 Fragments arranged by size: 2061 2042 734 648 359 159 82 15 MspA1I CmG'CkG Cuts at: 1606 2660 3135 3516 4193 5341 5586 1606 Size: 1054 475 381 677 1148 245 2120 Fragments arranged by size: 2120 1148 1054 677 475 381 245 NcoI C'CATG_G Cuts at: 513 1065 2148 3196 513 Size: 552 1083 1048 3417 Fragments arranged by size: 3417 1083 1048 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6100 NheI G'CTAG_C Cuts at: 1052 2597 1052 Size: 1545 4555 NotI GC'GGCC_GC Cuts at: 3547 3547 Size: 6100 NsiI A_TGCA'T Cuts at: 2872 2872 Size: 6100 NspI r_CATG'y Cuts at: 1132 1229 2506 3458 5003 1132 Size: 97 1277 952 1545 2229 Fragments arranged by size: 2229 1545 1277 952 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6100 PciI A'CATG_T Cuts at: 4999 4999 Size: 6100 PflMI CCAn_nnn'nTGG Cuts at: 1108 2725 1108 Size: 1617 4483 PfoI T'CCnGG_A Cuts at: 2179 2179 Size: 6100 PmeI GTTT'AAAC Cuts at: 3404 3404 Size: 6100 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4169 4201 5682 5714 4169 Size: 32 1481 32 4555 Fragments arranged by size: 4555 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6100 PsiI TTA'TAA Cuts at: 3670 3670 Size: 6100 PstI C_TGCA'G Cuts at: 830 2235 3140 3452 830 Size: 1405 905 312 3478 Fragments arranged by size: 3478 1405 905 312 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6100 PvuII CAG'CTG Cuts at: 2660 3135 2660 Size: 475 5625 SacI G_AGCT'C Cuts at: 3222 3222 Size: 6100 SalI G'TCGA_C Cuts at: 1936 3442 1936 Size: 1506 4594 SapI GCTCTTCn'nnn_ Cuts at: 2796 2796 Size: 6100 SbfI CC_TGCA'GG Cuts at: 2235 3452 2235 Size: 1217 4883 ScaI AGT'ACT Cuts at: 4348 6060 4348 Size: 1712 4388 SfcI C'TryA_G Cuts at: 826 1045 2231 3136 3448 4583 5264 5455 826 Size: 219 1186 905 312 1135 681 191 1471 Fragments arranged by size: 1471 1186 1135 905 681 312 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6100 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6100 SmaI CCC'GGG Cuts at: 1872 3427 1872 Size: 1555 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2170 4204 5105 5367 820 Size: 197 926 227 2034 901 262 1553 Fragments arranged by size: 2034 1553 926 901 262 227 197 SnaBI TAC'GTA Cuts at: 493 3891 493 Size: 3398 2702 SpeI A'CTAG_T Cuts at: 152 2036 2879 152 Size: 1884 843 3373 Fragments arranged by size: 3373 1884 843 SphI G_CATG'C Cuts at: 1132 3458 1132 Size: 2326 3774 SspI AAT'ATT Cuts at: 5 52 4024 5 Size: 47 3972 2081 Fragments arranged by size: 3972 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6100 StyI C'CwwG_G Cuts at: 513 1065 2148 3196 3539 513 Size: 552 1083 1048 343 3074 Fragments arranged by size: 3074 1083 1048 552 343 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4127 4144 4297 4482 4927 325 Size: 1255 2547 17 153 185 445 1498 Fragments arranged by size: 2547 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3012 3067 4346 Size: 274 80 33 51 1443 1035 55 1279 Cuts at: 4346 6058 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1279 1035 274 138 80 55 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2059 2091 2542 2574 6060 6092 2059 Size: 32 451 32 3486 32 2067 Fragments arranged by size: 3486 2067 451 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6100 XbaI T'CTAG_A Cuts at: 3436 3436 Size: 6100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2155 2011 Size: 144 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6100 XmaI C'CCGG_G Cuts at: 1870 3425 1870 Size: 1555 4545 XmnI GAAnn'nnTTC Cuts at: 2862 4229 2862 Size: 1367 4733 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2010 3908 276 Size: 53 83 186 936 476 1898 2468 Fragments arranged by size: 2468 1898 936 476 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI