Restriction map for
pFN21AB7706
(Linear) (Six-base) MAPSORT of: KIBB7706.seq Check: 4513 from: 1 to: 1395
KIBB7706.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AatII G_ACGT'C
Cuts at: 0 7 1395
Size: 7 1388
AccI GT'mk_AC
Cuts at: 0 824 1395
Size: 824 571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 1395
Size: 1315 80
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 983 1015 1395
Size: 983 32 380
Fragments arranged by size:
983 380 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1130 1173 1338 1395
Size: 1130 43 165 57
Fragments arranged by size:
1130 165 57 43
ApoI r'AATT_y
Cuts at: 0 331 436 1142 1395
Size: 331 105 706 253
Fragments arranged by size:
706 331 253 105
BanI G'GyrC_C
Cuts at: 0 139 1395
Size: 139 1256
BanII G_rGCy'C
Cuts at: 0 1217 1366 1395
Size: 1217 149 29
Fragments arranged by size:
1217 149 29
BciVI GTATCCnnnnn_n'
Cuts at: 0 717 1395
Size: 717 678
BclI T'GATC_A
Cuts at: 0 690 1395
Size: 690 705
BfrBI ATG'CAT
Cuts at: 0 865 1395
Size: 865 530
BglII A'GATC_T
Cuts at: 0 208 1395
Size: 208 1187
BlpI GC'TnA_GC
Cuts at: 0 1166 1395
Size: 1166 229
BmrI ACTGGGnnnn_n'
Cuts at: 0 752 1395
Size: 752 643
BmtI G_CTAG'C
Cuts at: 0 596 1395
Size: 596 799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 395 1395
Size: 175 220 1000
Fragments arranged by size:
1000 220 175
Bpu10I CC'TnA_GC
Cuts at: 0 478 1395
Size: 478 917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 186 1395
Size: 186 1209
BsaHI Gr'CG_yC
Cuts at: 0 4 222 1395
Size: 4 218 1173
Fragments arranged by size:
1173 218 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 651 681 1395
Size: 651 30 714
Fragments arranged by size:
714 651 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 94 656 1395
Size: 91 3 562 739
Fragments arranged by size:
739 562 91 3
BseYI C'CCAG_C
Cuts at: 0 615 651 713 1123 1395
Size: 615 36 62 410 272
Fragments arranged by size:
615 410 272 62 36
BsiHKAI G_wGCw'C
Cuts at: 0 1217 1395
Size: 1217 178
BsmI GAATG_Cn'
Cuts at: 0 1042 1395
Size: 1042 353
Bsp1286I G_dGCh'C
Cuts at: 0 1217 1366 1395
Size: 1217 149 29
Fragments arranged by size:
1217 149 29
BspHI T'CATG_A
Cuts at: 0 303 1395
Size: 303 1092
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 219 1395
Size: 219 1176
BsrFI r'CCGG_y
Cuts at: 0 1274 1395
Size: 1274 121
BsrGI T'GTAC_A
Cuts at: 0 1062 1395
Size: 1062 333
BstAPI GCAn_nnn'nTGC
Cuts at: 0 106 1395
Size: 106 1289
BstYI r'GATC_y
Cuts at: 0 208 343 900 1395
Size: 208 135 557 495
Fragments arranged by size:
557 495 208 135
BtgI C'CryG_G
Cuts at: 0 143 1191 1395
Size: 143 1048 204
Fragments arranged by size:
1048 204 143
DrdI GACnn_nn'nnGTC
Cuts at: 0 635 1395
Size: 635 760
EaeI y'GGCC_r
Cuts at: 0 11 283 1395
Size: 11 272 1112
Fragments arranged by size:
1112 272 11
EarI CTCTTCn'nnn_
Cuts at: 0 791 1334 1395
Size: 791 543 61
Fragments arranged by size:
791 543 61
EcoICRI GAG'CTC
Cuts at: 0 1215 1395
Size: 1215 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 395 1315 1395
Size: 175 220 920 80
Fragments arranged by size:
920 220 175 80
EcoRI G'AATT_C
Cuts at: 0 436 1395
Size: 436 959
HaeII r_GCGC'y
Cuts at: 0 282 1395
Size: 282 1113
HincII GTy'rAC
Cuts at: 0 1188 1395
Size: 1188 207
HindIII A'AGCT_T
Cuts at: 0 854 1395
Size: 854 541
MfeI C'AATT_G
Cuts at: 0 965 1395
Size: 965 430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 828 1282 1395
Size: 299 529 454 113
Fragments arranged by size:
529 454 299 113
MscI TGG'CCA
Cuts at: 0 13 1395
Size: 13 1382
MslI CAynn'nnrTG
Cuts at: 0 1222 1237 1319 1395
Size: 1222 15 82 76
Fragments arranged by size:
1222 82 76 15
MspA1I CmG'CkG
Cuts at: 0 655 1130 1395
Size: 655 475 265
Fragments arranged by size:
655 475 265
NcoI C'CATG_G
Cuts at: 0 143 1191 1395
Size: 143 1048 204
Fragments arranged by size:
1048 204 143
NheI G'CTAG_C
Cuts at: 0 592 1395
Size: 592 803
NsiI A_TGCA'T
Cuts at: 0 867 1395
Size: 867 528
NspI r_CATG'y
Cuts at: 0 501 1395
Size: 501 894
PflMI CCAn_nnn'nTGG
Cuts at: 0 720 1395
Size: 720 675
PfoI T'CCnGG_A
Cuts at: 0 174 1395
Size: 174 1221
PstI C_TGCA'G
Cuts at: 0 230 1135 1395
Size: 230 905 260
Fragments arranged by size:
905 260 230
PvuII CAG'CTG
Cuts at: 0 655 1130 1395
Size: 655 475 265
Fragments arranged by size:
655 475 265
SacI G_AGCT'C
Cuts at: 0 1217 1395
Size: 1217 178
SapI GCTCTTCn'nnn_
Cuts at: 0 791 1395
Size: 791 604
SbfI CC_TGCA'GG
Cuts at: 0 230 1395
Size: 230 1165
SfcI C'TryA_G
Cuts at: 0 226 1131 1395
Size: 226 905 264
Fragments arranged by size:
905 264 226
SmlI C'TyrA_G
Cuts at: 0 165 1395
Size: 165 1230
SpeI A'CTAG_T
Cuts at: 0 31 874 1395
Size: 31 843 521
Fragments arranged by size:
843 521 31
StyI C'CwwG_G
Cuts at: 0 143 1191 1395
Size: 143 1048 204
Fragments arranged by size:
1048 204 143
TatI w'GTAC_w
Cuts at: 0 1007 1062 1395
Size: 1007 55 333
Fragments arranged by size:
1007 333 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 54 86 537 569 1395
Size: 54 32 451 32 826
Fragments arranged by size:
826 451 54 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 1395
Size: 150 1245
XmnI GAAnn'nnTTC
Cuts at: 0 857 1395
Size: 857 538
ZraI GAC'GTC
Cuts at: 0 5 1395
Size: 5 1390
Enzymes that do cut:
AatII AccI AcuI AloI AlwNI ApoI BanI BanII
BciVI BclI BfrBI BglII BlpI BmrI BmtI BpmI
Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI BstYI BtgI
DrdI EaeI EarI EcoICRI Eco57MI EcoRI HaeII HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NheI
NsiI NspI PflMI PfoI PstI PvuII SacI SapI
SbfI SfcI SmlI SpeI StyI TatI TstI XcmI
XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BglI
Bme1580I BmgBI BplI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EciI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI Hin4I HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NotI NruI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XhoI XmaI