(Circular) (Six-base) MAPSORT of: pFN21AB6424.seq Check: 5895 from: 1 to: 5725 pFN21AB6424 5725 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2909 2909 Size: 5725 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3535 278 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2659 3068 1937 Size: 722 409 4594 Fragments arranged by size: 4594 722 409 Acc65I G'GTAC_C Cuts at: 3046 3046 Size: 5725 AclI AA'CG_TT Cuts at: 1563 3852 4225 1563 Size: 2289 373 3063 Fragments arranged by size: 3063 2289 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3788 5172 5440 1632 Size: 2156 1384 268 1917 Fragments arranged by size: 2156 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5528 AflIII A'CryG_T Cuts at: 1203 2677 4624 5601 1203 Size: 1474 1947 977 1327 Fragments arranged by size: 1947 1474 1327 977 AgeI A'CCGG_T Cuts at: 4556 4556 Size: 5725 AhdI GACnn_n'nnGTC Cuts at: 4454 4454 Size: 5725 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5725 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5725 AlwNI CAG_nnn'CTG Cuts at: 3468 5040 3468 Size: 1572 4153 ApaLI G'TGCA_C Cuts at: 3782 4938 3782 Size: 1156 4569 ApoI r'AATT_y Cuts at: 1493 3034 3260 3412 5521 1493 Size: 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 226 152 AseI AT'TA_AT Cuts at: 160 1032 2240 4279 160 Size: 872 1208 2039 1606 Fragments arranged by size: 2039 1606 1208 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 AvaI C'yCGr_G Cuts at: 1870 1943 3050 1870 Size: 73 1107 4545 Fragments arranged by size: 4545 1107 73 BamHI G'GATC_C Cuts at: 3055 3422 3055 Size: 367 5358 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3046 4501 618 Size: 325 217 627 1259 1455 1842 Fragments arranged by size: 1842 1455 1259 627 325 217 BanII G_rGCy'C Cuts at: 1391 3044 1391 Size: 1653 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4754 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3916 3950 1903 Size: 34 1979 34 3678 Fragments arranged by size: 3678 1979 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2189 3617 4827 2189 Size: 1428 1210 3087 Fragments arranged by size: 3087 1428 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5725 BfrBI ATG'CAT Cuts at: 2188 2275 2188 Size: 87 5638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4336 136 Size: 107 122 71 631 605 2664 1525 Fragments arranged by size: 2664 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5720 5720 Size: 5725 BlpI GC'TnA_GC Cuts at: 3142 3142 Size: 5725 Bme1580I G_kGCm'C Cuts at: 1790 3786 4942 1790 Size: 1996 1156 2573 Fragments arranged by size: 2573 1996 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5725 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2556 3456 4414 Size: 416 53 83 399 24 1139 900 958 Cuts at: 4414 442 Size: 1753 Fragments arranged by size: 1753 1139 958 900 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5725 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2577 4385 1239 Size: 1338 1808 2579 Fragments arranged by size: 2579 1808 1338 Bpu10I CC'TnA_GC Cuts at: 2956 2956 Size: 5725 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2211 3850 4715 5013 2211 Size: 1639 865 298 2923 Fragments arranged by size: 2923 1639 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4388 882 Size: 617 2889 2219 Fragments arranged by size: 2889 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3516 493 Size: 711 2312 2702 Fragments arranged by size: 2702 2312 711 BsaBI GATnn'nnATC Cuts at: 5719 5719 Size: 5725 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2851 3532 3914 4549 Size: 53 83 186 936 1318 681 382 635 Cuts at: 4549 275 Size: 1451 Fragments arranged by size: 1451 1318 936 681 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4158 4556 4830 4977 5388 4158 Size: 398 274 147 411 4495 Fragments arranged by size: 4495 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5500 Fragments arranged by size: 5500 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5725 BseYI C'CCAG_C Cuts at: 2526 4928 5424 2526 Size: 2402 496 2827 Fragments arranged by size: 2827 2402 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2793 2937 1163 Size: 573 1057 144 3951 Fragments arranged by size: 3951 1057 573 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3175 3936 4964 1540 Size: 275 186 1174 761 1028 2301 Fragments arranged by size: 2301 1174 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3786 3871 4942 3786 Size: 85 1071 4569 Fragments arranged by size: 4569 1071 85 BsmI GAATG_Cn' Cuts at: 2551 3236 3329 2551 Size: 685 93 4947 Fragments arranged by size: 4947 685 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2858 2858 Size: 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3044 3786 3871 4942 1391 Size: 399 1254 742 85 1071 2174 Fragments arranged by size: 2174 1254 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5388 5388 Size: 5725 BspHI T'CATG_A Cuts at: 2800 2935 3614 5344 2800 Size: 135 679 1730 3181 Fragments arranged by size: 3181 1730 679 135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2909 3080 844 Size: 2065 171 3489 Fragments arranged by size: 3489 2065 171 BsrBI CCG'CTC Cuts at: 1394 1823 3612 1394 Size: 429 1789 3507 Fragments arranged by size: 3507 1789 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4220 4394 65 Size: 1183 2972 174 1396 Fragments arranged by size: 2972 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2754 4369 4556 1700 Size: 110 944 1615 187 2869 Fragments arranged by size: 2869 1615 944 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5725 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5725 BssSI C'ACGA_G Cuts at: 3785 4797 3785 Size: 1012 4713 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5725 BstBI TT'CG_AA Cuts at: 4529 4529 Size: 5725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5725 BstYI r'GATC_y Cuts at: 1972 3055 3422 3807 3824 5276 5362 5374 5720 Size: 1083 367 385 17 1452 86 12 346 Cuts at: 5720 1972 Size: 1977 Fragments arranged by size: 1977 1452 1083 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5173 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4986 Fragments arranged by size: 4986 683 56 BtsI GCAGTG_nn' Cuts at: 3241 4035 4055 3241 Size: 794 20 4911 Fragments arranged by size: 4911 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5725 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5690 DraI TTT'AAA Cuts at: 3029 3029 Size: 5725 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4732 809 Size: 507 3416 1802 Fragments arranged by size: 3416 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3172 4061 5690 Size: 54 1425 50 275 18 1342 889 1629 Cuts at: 5690 8 Size: 43 Fragments arranged by size: 1629 1425 1342 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3172 1537 Size: 275 1360 4090 Fragments arranged by size: 4090 1360 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2629 3655 1353 Size: 1276 1026 3423 Fragments arranged by size: 3423 1276 1026 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4312 4686 4832 387 Size: 1064 138 2723 374 146 1280 Fragments arranged by size: 2723 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2577 3788 4385 5172 5440 1239 Size: 393 945 1211 597 787 268 1524 Fragments arranged by size: 1524 1211 945 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2292 2292 Size: 5725 EcoRI G'AATT_C Cuts at: 3034 3412 3034 Size: 378 5347 EcoRV GAT'ATC Cuts at: 2689 2689 Size: 5725 FspI TGC'GCA Cuts at: 4231 4231 Size: 5725 HaeII r_GCGC'y Cuts at: 1678 4872 1678 Size: 3194 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373 Size: 32 135 32 2794 32 42 32 2626 Fragments arranged by size: 2794 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3069 3315 669 Size: 988 281 1131 246 3079 Fragments arranged by size: 3079 1131 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5725 HpaI GTT'AAC Cuts at: 3315 3315 Size: 5725 KpnI G_GTAC'C Cuts at: 3050 3050 Size: 5725 MfeI C'AATT_G Cuts at: 3324 3324 Size: 5725 MluI A'CGCG_T Cuts at: 5601 5601 Size: 5725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4839 5023 1305 Size: 3534 184 2007 Fragments arranged by size: 3534 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3903 Fragments arranged by size: 3903 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2981 3683 4042 4201 518 Size: 648 1815 702 359 159 2042 Fragments arranged by size: 2042 1815 702 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3141 3818 4966 5211 1606 Size: 1535 677 1148 245 2120 Fragments arranged by size: 2120 1535 1148 677 245 NaeI GCC'GGC Cuts at: 2756 2756 Size: 5725 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5173 NdeI CA'TA_TG Cuts at: 387 387 Size: 5725 NgoMIV G'CCGG_C Cuts at: 2754 2754 Size: 5725 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5725 NotI GC'GGCC_GC Cuts at: 3172 3172 Size: 5725 NsiI A_TGCA'T Cuts at: 2190 2277 2190 Size: 87 5638 NspI r_CATG'y Cuts at: 1132 1229 2079 2279 2681 3083 4628 1132 Size: 97 850 200 402 402 1545 2229 Fragments arranged by size: 2229 1545 850 402 402 200 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5725 PciI A'CATG_T Cuts at: 2677 4624 2677 Size: 1947 3778 PflMI CCAn_nnn'nTGG Cuts at: 1108 2634 1108 Size: 1526 4199 PmeI GTTT'AAAC Cuts at: 3029 3029 Size: 5725 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3794 3826 5307 5339 3794 Size: 32 1481 32 4180 Fragments arranged by size: 4180 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2292 2292 Size: 5725 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5725 PsiI TTA'TAA Cuts at: 3295 3295 Size: 5725 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2134 2166 2134 Size: 32 5693 PstI C_TGCA'G Cuts at: 830 3077 830 Size: 2247 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5725 SalI G'TCGA_C Cuts at: 1936 3067 1936 Size: 1131 4594 SbfI CC_TGCA'GG Cuts at: 3077 3077 Size: 5725 ScaI AGT'ACT Cuts at: 3973 5685 3973 Size: 1712 4013 SfcI C'TryA_G Cuts at: 826 1045 2829 3073 4208 4889 5080 826 Size: 219 1784 244 1135 681 191 1471 Fragments arranged by size: 1784 1471 1135 681 244 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5725 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5725 SmaI CCC'GGG Cuts at: 1872 3052 1872 Size: 1180 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2226 3829 4730 4992 820 Size: 197 926 283 1603 901 262 1553 Fragments arranged by size: 1603 1553 926 901 283 262 197 SnaBI TAC'GTA Cuts at: 493 3516 493 Size: 3023 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5725 SphI G_CATG'C Cuts at: 1132 2279 3083 1132 Size: 1147 804 3774 Fragments arranged by size: 3774 1147 804 SspI AAT'ATT Cuts at: 5 52 3649 5 Size: 47 3597 2081 Fragments arranged by size: 3597 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5725 StyI C'CwwG_G Cuts at: 513 1065 3164 513 Size: 552 2099 3074 Fragments arranged by size: 3074 2099 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2290 3752 3769 3922 4107 4552 325 Size: 1255 710 1462 17 153 185 445 1498 Fragments arranged by size: 1498 1462 1255 710 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3971 5683 96 Size: 274 80 33 51 1443 1994 1712 138 Fragments arranged by size: 1994 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5685 5717 5685 Size: 32 5693 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5725 XbaI T'CTAG_A Cuts at: 3061 3061 Size: 5725 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5725 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5725 XmaI C'CCGG_G Cuts at: 1870 3050 1870 Size: 1180 4545 XmnI GAAnn'nnTTC Cuts at: 2824 3854 2824 Size: 1030 4695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3533 276 Size: 53 83 186 936 1999 2468 Fragments arranged by size: 2468 1999 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI