(Circular) (Six-base) MAPSORT of: pFN21AB6524.seq Check: 3143 from: 1 to: 5866 pFN21AB6524 5866 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2470 2470 Size: 5866 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2592 3209 1937 Size: 655 617 4594 Fragments arranged by size: 4594 655 617 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2498 2654 2984 3929 5313 5581 1632 Size: 866 156 330 945 1384 268 1917 Fragments arranged by size: 1917 1384 945 866 330 268 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 4765 5742 1203 Size: 3562 977 1327 Fragments arranged by size: 3562 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 3105 1166 Size: 1939 3927 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 2362 3609 5181 2362 Size: 1247 1572 3047 Fragments arranged by size: 3047 1572 1247 ApaLI G'TGCA_C Cuts at: 2114 3098 3923 5079 2114 Size: 984 825 1156 2901 Fragments arranged by size: 2901 1156 984 825 ApoI r'AATT_y Cuts at: 1493 3175 3401 3553 5662 1493 Size: 1682 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 3191 1870 Size: 73 1248 4545 Fragments arranged by size: 4545 1248 73 BamHI G'GATC_C Cuts at: 2805 3196 3563 2805 Size: 391 367 5108 Fragments arranged by size: 5108 391 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2713 2785 2890 3061 3142 Size: 325 217 627 926 72 105 171 81 Cuts at: 3142 3187 4642 618 Size: 45 1455 1842 Fragments arranged by size: 1842 1455 926 627 325 217 171 105 81 72 45 BanII G_rGCy'C Cuts at: 1391 2304 2505 3185 1391 Size: 913 201 680 4072 Fragments arranged by size: 4072 913 680 201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4895 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2227 4477 136 Size: 107 122 71 631 605 555 2250 1525 Fragments arranged by size: 2250 1525 631 605 555 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 3283 3283 Size: 5866 Bme1580I G_kGCm'C Cuts at: 1790 2118 2674 2790 2895 3066 3102 3147 3927 Size: 328 556 116 105 171 36 45 780 Cuts at: 3927 5083 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 780 556 328 171 116 105 45 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 2288 2390 2831 4526 1239 Size: 947 102 102 441 1695 2579 Fragments arranged by size: 2579 1695 947 441 102 102 Bpu10I CC'TnA_GC Cuts at: 2151 2151 Size: 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2964 4529 882 Size: 617 1465 1565 2219 Fragments arranged by size: 2219 1565 1465 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 5860 5860 Size: 5866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3673 4055 4690 275 Size: 53 83 186 936 2140 382 635 1451 Fragments arranged by size: 2140 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4299 4697 4971 5118 5529 4299 Size: 398 274 147 411 4636 Fragments arranged by size: 4636 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5641 Fragments arranged by size: 5641 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2019 2024 2456 3044 1186 Size: 833 5 432 588 4008 Fragments arranged by size: 4008 833 588 432 5 BseYI C'CCAG_C Cuts at: 2056 2073 2241 5069 5565 2056 Size: 17 168 2828 496 2357 Fragments arranged by size: 2828 2357 496 168 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2992 1163 Size: 573 1256 4037 Fragments arranged by size: 4037 1256 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 4077 5105 1540 Size: 275 186 1315 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 3102 3927 4012 5083 2118 Size: 984 825 85 1071 2901 Fragments arranged by size: 2901 1071 984 825 85 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 BsmBI CGTCTCn'nnnn_ Cuts at: 2126 2126 Size: 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2304 2505 2674 2790 2895 3066 Size: 399 328 186 201 169 116 105 171 Cuts at: 3066 3102 3147 3185 3927 4012 5083 1391 Size: 36 45 38 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 201 186 171 169 116 105 85 45 38 36 BspEI T'CCGG_A Cuts at: 5529 5529 Size: 5866 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2226 2470 3221 844 Size: 1382 244 751 3489 Fragments arranged by size: 3489 1382 751 244 BsrBI CCG'CTC Cuts at: 1394 1823 3112 3753 1394 Size: 429 1289 641 3507 Fragments arranged by size: 3507 1289 641 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2746 4361 4535 65 Size: 1183 1498 1615 174 1396 Fragments arranged by size: 1615 1498 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2946 4510 4697 1700 Size: 110 1136 1564 187 2869 Fragments arranged by size: 2869 1564 1136 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5866 BssSI C'ACGA_G Cuts at: 2036 3926 4938 2036 Size: 1890 1012 2964 Fragments arranged by size: 2964 1890 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5866 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 2878 1184 Size: 1694 4172 BstXI CCAn_nnnn'nTGG Cuts at: 2261 2261 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2805 3196 3563 3948 3965 5417 5503 5515 Size: 833 391 367 385 17 1452 86 12 Cuts at: 5515 5861 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 833 391 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2593 2593 Size: 5866 BtgI C'CryG_G Cuts at: 513 1065 2004 2545 3016 513 Size: 552 939 541 471 3363 Fragments arranged by size: 3363 939 552 541 471 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3004 3382 4176 4196 3004 Size: 378 794 20 4674 Fragments arranged by size: 4674 794 378 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4873 809 Size: 507 3557 1802 Fragments arranged by size: 3557 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3048 3068 3313 Size: 54 1425 50 275 18 1218 20 245 Cuts at: 3313 4202 5831 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1218 889 275 245 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 1537 Size: 275 1501 4090 Fragments arranged by size: 4090 1501 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2960 3796 1353 Size: 671 936 836 3423 Fragments arranged by size: 3423 936 836 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4453 4827 4973 387 Size: 1064 138 2864 374 146 1280 Fragments arranged by size: 2864 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2288 2390 2498 2654 2831 2984 Size: 393 554 102 102 108 156 177 153 Cuts at: 2984 3929 4526 5313 5581 1239 Size: 945 597 787 268 1524 Fragments arranged by size: 1524 945 787 597 554 393 268 177 156 153 108 102 102 EcoO109I rG'GnC_Cy Cuts at: 2380 2390 2845 2380 Size: 10 455 5401 Fragments arranged by size: 5401 455 10 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FspI TGC'GCA Cuts at: 2426 4372 2426 Size: 1946 3920 HaeII r_GCGC'y Cuts at: 1678 3138 5013 1678 Size: 1460 1875 2531 Fragments arranged by size: 2531 1875 1460 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4507 4539 4581 4613 1373 Size: 32 135 32 2935 32 42 32 2626 Fragments arranged by size: 2935 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2731 3210 3456 669 Size: 988 281 793 479 246 3079 Fragments arranged by size: 3079 988 793 479 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3009 4980 5164 1305 Size: 1704 1971 184 2007 Fragments arranged by size: 2007 1971 1704 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4044 Fragments arranged by size: 4044 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2600 2993 3105 3824 4183 4342 518 Size: 648 1434 393 112 719 359 159 2042 Fragments arranged by size: 2042 1434 719 648 393 359 159 112 MspA1I CmG'CkG Cuts at: 1606 2174 2518 2667 3282 3959 5107 5352 1606 Size: 568 344 149 615 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 615 568 344 245 149 NaeI GCC'GGC Cuts at: 2948 2948 Size: 5866 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4375 Fragments arranged by size: 4375 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NgoMIV G'CCGG_C Cuts at: 2946 2946 Size: 5866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 2159 3224 4769 1132 Size: 97 930 1065 1545 2229 Fragments arranged by size: 2229 1545 1065 930 97 PasI CC'CwG_GG Cuts at: 1348 2674 1348 Size: 1326 4540 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 2801 1108 Size: 1693 4173 PfoI T'CCnGG_A Cuts at: 2808 2808 Size: 5866 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2380 2380 Size: 5866 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PstI C_TGCA'G Cuts at: 830 2779 3218 830 Size: 1949 439 3478 Fragments arranged by size: 3478 1949 439 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 PvuII CAG'CTG Cuts at: 2518 2518 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SanDI GG'GwC_CC Cuts at: 2380 2380 Size: 5866 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 2775 3214 4349 5030 5221 826 Size: 219 1730 439 1135 681 191 1471 Fragments arranged by size: 1730 1471 1135 681 439 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 3193 1872 Size: 1321 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3970 4871 5133 820 Size: 197 926 2027 901 262 1553 Fragments arranged by size: 2027 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2484 2538 2709 3305 513 Size: 552 939 117 363 54 171 596 3074 Fragments arranged by size: 3074 939 596 552 363 171 117 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 2226 2255 2851 3893 3910 4063 Size: 1255 526 120 29 596 1042 17 153 Cuts at: 4063 4248 4693 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1042 596 526 445 185 153 120 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4112 5824 96 Size: 274 80 33 51 1443 2135 1712 138 Fragments arranged by size: 2135 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2160 2192 2706 2738 5826 5858 2160 Size: 32 514 32 3088 32 2168 Fragments arranged by size: 3088 2168 514 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 3191 1870 Size: 1321 4545 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI