Restriction map for pFN21AB6466
 (Circular) (Six-base) MAPSORT of: pFN21AB6466.seq  Check: 5613  from: 1  to: 5761



pFN21AB6466  5761 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:40  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3571     278 
   Size:         53      83     186     936    2035    2468

  Fragments arranged by size: 

                2468    2035     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3104    1937 
   Size:       1167    4594
 
Acc65I G'GTAC_C

Cuts at:   2377    3082    2377 
   Size:        705    5056
 
AclI AA'CG_TT

Cuts at:   1563    3888    4261    1563 
   Size:       2325     373    3063

  Fragments arranged by size: 

                3063    2325     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2198    2421    2625    3074    3824    5208    5476    1632 
   Size:        566     223     204     449     750    1384     268    1917

  Fragments arranged by size: 

                1917    1384     750     566     449     268     223     204
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5564
 
AflIII A'CryG_T

Cuts at:   1203    2817    4660    5637    1203 
   Size:       1614    1843     977    1327

  Fragments arranged by size: 

                1843    1614    1327     977
 
AgeI A'CCGG_T

Cuts at:   4592    4592 
   Size:       5761
 
AhdI GACnn_n'nnGTC

Cuts at:   4490    4490 
   Size:       5761
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5761
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5761
 
AlwNI CAG_nnn'CTG

Cuts at:   2836    3504    5076    2836 
   Size:        668    1572    3521

  Fragments arranged by size: 

                3521    1572     668
 
ApaI G_GGCC'C

Cuts at:   2090    2114    2090 
   Size:         24    5737
 
ApaLI G'TGCA_C

Cuts at:   3818    4974    3818 
   Size:       1156    4605
 
ApoI r'AATT_y

Cuts at:   1493    3070    3296    3448    5557    1493 
   Size:       1577     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1577     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4315     160 
   Size:        872    3283    1606

  Fragments arranged by size: 

                3283    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5761
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2083    2113    2715    2973    3086    1870 
   Size:         73     140      30     602     258     113    4545

  Fragments arranged by size: 

                4545     602     258     140     113      73      30
 
AvrII C'CTAG_G

Cuts at:   2881    2881 
   Size:       5761
 
BamHI G'GATC_C

Cuts at:   3091    3458    3091 
   Size:        367    5394
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2377    3082    4537     618 
   Size:        325     217     627     590     705    1455    1842

  Fragments arranged by size: 

                1842    1455     705     627     590     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2090    2114    2183    2576    3000    3080    1391 
   Size:        699      24      69     393     424      80    4072

  Fragments arranged by size: 

                4072     699     424     393      80      69      24
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2893     928 
   Size:        971     994    3796

  Fragments arranged by size: 

                3796     994     971
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2519    2553    3952    3986    1903 
   Size:         34     582      34    1399      34    3678

  Fragments arranged by size: 

                3678    1399     582      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3653    4863    3653 
   Size:       1210    4551
 
BclI T'GATC_A

Cuts at:   1548    2892    1548 
   Size:       1344    4417
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4372     136 
   Size:        107     122      71     631     605    2700    1525

  Fragments arranged by size: 

                2700    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2163    5756    2163 
   Size:       3593    2168
 
BlpI GC'TnA_GC

Cuts at:   3178    3178 
   Size:       5761
 
Bme1580I G_kGCm'C

Cuts at:   1790    2090    2114    3822    4978    1790 
   Size:        300      24    1708    1156    2573

  Fragments arranged by size: 

                2573    1708    1156     300      24
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5761
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3492    4450     442 
   Size:        416      53      83     399      24    2075     958    1753

  Fragments arranged by size: 

                2075    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5761
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2560    4421    1239 
   Size:       1321    1861    2579

  Fragments arranged by size: 

                2579    1861    1321
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2502    3886    4751    5049    2502 
   Size:       1384     865     298    3214

  Fragments arranged by size: 

                3214    1384     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4424     882 
   Size:        617    2925    2219

  Fragments arranged by size: 

                2925    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3552     493 
   Size:        711    2348    2702

  Fragments arranged by size: 

                2702    2348     711
 
BsaBI GATnn'nnATC

Cuts at:   5755    5755 
   Size:       5761
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2358    2766    3568    3950 
   Size:         53      83     186     936     825     408     802     382

Cuts at:   3950    4585     275 
   Size:        635    1451

  Fragments arranged by size: 

                1451     936     825     802     635     408     382     186
                  83      53
 
BsaWI w'CCGG_w

Cuts at:   4194    4592    4866    5013    5424    4194 
   Size:        398     274     147     411    4531

  Fragments arranged by size: 

                4531     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2771    2801    2987    3017    1178 
   Size:         30     165      30    1368      30     186      30    3922

  Fragments arranged by size: 

                3922    1368     186     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3008    1186 
   Size:       1822    3939
 
BseYI C'CCAG_C

Cuts at:   2306    2987    4964    5460    2306 
   Size:        681    1977     496    2607

  Fragments arranged by size: 

                2607    1977     681     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2915    1163 
   Size:        573    1179    4009

  Fragments arranged by size: 

                4009    1179     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3211    3972    5000    1540 
   Size:        275     186    1210     761    1028    2301

  Fragments arranged by size: 

                2301    1210    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2576    3000    3822    3907    4978    2576 
   Size:        424     822      85    1071    3359

  Fragments arranged by size: 

                3359    1071     822     424      85
 
BsmI GAATG_Cn'

Cuts at:   2205    3272    3365    2205 
   Size:       1067      93    4601

  Fragments arranged by size: 

                4601    1067      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2090    2114    2183    2576    3000    3080    3822 
   Size:        399     300      24      69     393     424      80     742

Cuts at:   3822    3907    4978    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     424     399     393     300      85
                  80      69      24
 
BspEI T'CCGG_A

Cuts at:   5424    5424 
   Size:       5761
 
BspHI T'CATG_A

Cuts at:   3650    5380    3650 
   Size:       1730    4031
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3116     844 
   Size:       2272    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2104    3648    1394 
   Size:        429     281    1544    3507

  Fragments arranged by size: 

                3507    1544     429     281
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4256    4430      65 
   Size:       1183    3008     174    1396

  Fragments arranged by size: 

                3008    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4405    4592    1700 
   Size:        110    2595     187    2869

  Fragments arranged by size: 

                2869    2595     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5761
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5761
 
BssSI C'ACGA_G

Cuts at:   3821    4833    3821 
   Size:       1012    4749
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5761
 
BstBI TT'CG_AA

Cuts at:   4565    4565 
   Size:       5761
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5761
 
BstYI r'GATC_y

Cuts at:   1972    2163    3091    3458    3843    3860    5312    5398    5410 
   Size:        191     928     367     385      17    1452      86      12

Cuts at:   5410    5756    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     928     385     367     346     191      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2740    2805    3047     513 
   Size:        552     939     736      65     242    3227

  Fragments arranged by size: 

                3227     939     736     552     242      65
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2443    2950    2965     487 
   Size:        683      56    1217     507      15    3283

  Fragments arranged by size: 

                3283    1217     683     507      56      15
 
BtsI GCAGTG_nn'

Cuts at:   3277    4071    4091    3277 
   Size:        794      20    4947

  Fragments arranged by size: 

                4947     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    2673    1554 
   Size:       1119    4642
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5726
 
DraI TTT'AAA

Cuts at:   3065    3065 
   Size:       5761
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4768     809 
   Size:        507    3452    1802

  Fragments arranged by size: 

                3452    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3208    4097    5726 
   Size:         54    1425      50     275      18    1378     889    1629

Cuts at:   5726       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1378     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3208    1537 
   Size:        275    1396    4090

  Fragments arranged by size: 

                4090    1396     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2214    2286    2576    3691    1353 
   Size:        861      72     290    1115    3423

  Fragments arranged by size: 

                3423    1115     861     290      72
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4348    4722    4868     387 
   Size:       1064     138    2759     374     146    1280

  Fragments arranged by size: 

                2759    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2574    2998    2574 
   Size:        424    5337
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2198    2421    2560    2625    3074    3824    4421 
   Size:        393     566     223     139      65     449     750     597

Cuts at:   4421    5208    5476    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     787     750     597     566     449     393     268
                 223     139      65
 
EcoO109I rG'GnC_Cy

Cuts at:   2110    2121    2369    2500    2746    2797    2110 
   Size:         11     248     131     246      51    5074

  Fragments arranged by size: 

                5074     248     246     131      51      11
 
EcoRI G'AATT_C

Cuts at:   3070    3448    3070 
   Size:        378    5383
 
FspI TGC'GCA

Cuts at:   4267    4267 
   Size:       5761
 
HaeII r_GCGC'y

Cuts at:   1678    2328    4908    1678 
   Size:        650    2580    2531

  Fragments arranged by size: 

                2580    2531     650
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2585    2617    4402    4434    4476 
   Size:         32     135      32    1013      32    1785      32      42

Cuts at:   4476    4508    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1785    1013     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3105    3351     669 
   Size:        988     281    1167     246    3079

  Fragments arranged by size: 

                3079    1167     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5761
 
HpaI GTT'AAC

Cuts at:   3351    3351 
   Size:       5761
 
KpnI G_GTAC'C

Cuts at:   2381    3086    2381 
   Size:        705    5056
 
MfeI C'AATT_G

Cuts at:   3360    3360 
   Size:       5761
 
MluI A'CGCG_T

Cuts at:   5637    5637 
   Size:       5761
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4875    5059    1305 
   Size:       3570     184    2007

  Fragments arranged by size: 

                3570    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3939

  Fragments arranged by size: 

                3939    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3719    4078    4237     518 
   Size:        648    2553     359     159    2042

  Fragments arranged by size: 

                2553    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3177    3854    5002    5247    1606 
   Size:       1571     677    1148     245    2120

  Fragments arranged by size: 

                2120    1571    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4270

  Fragments arranged by size: 

                4270     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5761
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5761
 
NotI GC'GGCC_GC

Cuts at:   3208    3208 
   Size:       5761
 
NspI r_CATG'y

Cuts at:   1132    1229    2821    3062    3119    4664    1132 
   Size:         97    1592     241      57    1545    2229

  Fragments arranged by size: 

                2229    1592    1545     241      97      57
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5761
 
PciI A'CATG_T

Cuts at:   2817    4660    2817 
   Size:       1843    3918
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5761
 
PfoI T'CCnGG_A

Cuts at:   2576    2576 
   Size:       5761
 
PmeI GTTT'AAAC

Cuts at:   3065    3065 
   Size:       5761
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2177    2209    3830    3862    5343    5375    2177 
   Size:         32    1621      32    1481      32    2563

  Fragments arranged by size: 

                2563    1621    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2369    2746    2797    2369 
   Size:        377      51    5333

  Fragments arranged by size: 

                5333     377      51
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5761
 
PsiI TTA'TAA

Cuts at:   3331    3331 
   Size:       5761
 
PspOMI G'GGCC_C

Cuts at:   2086    2110    2086 
   Size:         24    5737
 
PstI C_TGCA'G

Cuts at:    830    2601    3113     830 
   Size:       1771     512    3478

  Fragments arranged by size: 

                3478    1771     512
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5761
 
SacI G_AGCT'C

Cuts at:   2576    3000    2576 
   Size:        424    5337
 
SalI G'TCGA_C

Cuts at:   1936    3103    1936 
   Size:       1167    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2576    2576 
   Size:       5761
 
SbfI CC_TGCA'GG

Cuts at:   3113    3113 
   Size:       5761
 
ScaI AGT'ACT

Cuts at:   4009    5721    4009 
   Size:       1712    4049
 
SfcI C'TryA_G

Cuts at:    826    1045    2278    2597    3109    4244    4925    5116     826 
   Size:        219    1233     319     512    1135     681     191    1471

  Fragments arranged by size: 

                1471    1233    1135     681     512     319     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5761
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5761
 
SmaI CCC'GGG

Cuts at:   1872    2085    2115    2717    3088    1872 
   Size:        213      30     602     371    4545

  Fragments arranged by size: 

                4545     602     371     213      30
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2517    3865    4766    5028     820 
   Size:        197     926     574    1348     901     262    1553

  Fragments arranged by size: 

                1553    1348     926     901     574     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3552     493 
   Size:       3059    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5761
 
SphI G_CATG'C

Cuts at:   1132    3119    1132 
   Size:       1987    3774
 
SspI AAT'ATT

Cuts at:      5      52    3685       5 
   Size:         47    3633    2081

  Fragments arranged by size: 

                3633    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5761
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2881    3200     513 
   Size:        552     939     877     319    3074

  Fragments arranged by size: 

                3074     939     877     552     319
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3788    3805    3958    4143    4588     325 
   Size:       1255    2208      17     153     185     445    1498

  Fragments arranged by size: 

                2208    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2820    4007    5719 
   Size:        274      80      33      51    1443     843    1187    1712

Cuts at:   5719      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1187     843     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5721    5753    5721 
   Size:         32    5729
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5761
 
XbaI T'CTAG_A

Cuts at:   3097    3097 
   Size:       5761
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5761
 
XmaI C'CCGG_G

Cuts at:   1870    2083    2113    2715    3086    1870 
   Size:        213      30     602     371    4545

  Fragments arranged by size: 

                4545     602     371     213      30
 
XmnI GAAnn'nnTTC

Cuts at:   3890    3890 
   Size:       5761
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3569     276 
   Size:         53      83     186     936    2035    2468

  Fragments arranged by size: 

                2468    2035     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
     PstI     PvuI     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI     BaeI     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PmlI    PspXI     PsrI    PvuII
    RsrII    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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