(Circular) (Six-base) MAPSORT of: pFN21AB6466.seq Check: 5613 from: 1 to: 5761 pFN21AB6466 5761 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 2377 3082 2377 Size: 705 5056 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2198 2421 2625 3074 3824 5208 5476 1632 Size: 566 223 204 449 750 1384 268 1917 Fragments arranged by size: 1917 1384 750 566 449 268 223 204 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 2817 4660 5637 1203 Size: 1614 1843 977 1327 Fragments arranged by size: 1843 1614 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2836 3504 5076 2836 Size: 668 1572 3521 Fragments arranged by size: 3521 1572 668 ApaI G_GGCC'C Cuts at: 2090 2114 2090 Size: 24 5737 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 3070 3296 3448 5557 1493 Size: 1577 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2083 2113 2715 2973 3086 1870 Size: 73 140 30 602 258 113 4545 Fragments arranged by size: 4545 602 258 140 113 73 30 AvrII C'CTAG_G Cuts at: 2881 2881 Size: 5761 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2377 3082 4537 618 Size: 325 217 627 590 705 1455 1842 Fragments arranged by size: 1842 1455 705 627 590 325 217 BanII G_rGCy'C Cuts at: 1391 2090 2114 2183 2576 3000 3080 1391 Size: 699 24 69 393 424 80 4072 Fragments arranged by size: 4072 699 424 393 80 69 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2893 928 Size: 971 994 3796 Fragments arranged by size: 3796 994 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2519 2553 3952 3986 1903 Size: 34 582 34 1399 34 3678 Fragments arranged by size: 3678 1399 582 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 2892 1548 Size: 1344 4417 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2163 5756 2163 Size: 3593 2168 BlpI GC'TnA_GC Cuts at: 3178 3178 Size: 5761 Bme1580I G_kGCm'C Cuts at: 1790 2090 2114 3822 4978 1790 Size: 300 24 1708 1156 2573 Fragments arranged by size: 2573 1708 1156 300 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2560 4421 1239 Size: 1321 1861 2579 Fragments arranged by size: 2579 1861 1321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2502 3886 4751 5049 2502 Size: 1384 865 298 3214 Fragments arranged by size: 3214 1384 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2358 2766 3568 3950 Size: 53 83 186 936 825 408 802 382 Cuts at: 3950 4585 275 Size: 635 1451 Fragments arranged by size: 1451 936 825 802 635 408 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2771 2801 2987 3017 1178 Size: 30 165 30 1368 30 186 30 3922 Fragments arranged by size: 3922 1368 186 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3008 1186 Size: 1822 3939 BseYI C'CCAG_C Cuts at: 2306 2987 4964 5460 2306 Size: 681 1977 496 2607 Fragments arranged by size: 2607 1977 681 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2915 1163 Size: 573 1179 4009 Fragments arranged by size: 4009 1179 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2576 3000 3822 3907 4978 2576 Size: 424 822 85 1071 3359 Fragments arranged by size: 3359 1071 822 424 85 BsmI GAATG_Cn' Cuts at: 2205 3272 3365 2205 Size: 1067 93 4601 Fragments arranged by size: 4601 1067 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2114 2183 2576 3000 3080 3822 Size: 399 300 24 69 393 424 80 742 Cuts at: 3822 3907 4978 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 393 300 85 80 69 24 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2104 3648 1394 Size: 429 281 1544 3507 Fragments arranged by size: 3507 1544 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4405 4592 1700 Size: 110 2595 187 2869 Fragments arranged by size: 2869 2595 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstYI r'GATC_y Cuts at: 1972 2163 3091 3458 3843 3860 5312 5398 5410 Size: 191 928 367 385 17 1452 86 12 Cuts at: 5410 5756 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 928 385 367 346 191 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2740 2805 3047 513 Size: 552 939 736 65 242 3227 Fragments arranged by size: 3227 939 736 552 242 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2443 2950 2965 487 Size: 683 56 1217 507 15 3283 Fragments arranged by size: 3283 1217 683 507 56 15 BtsI GCAGTG_nn' Cuts at: 3277 4071 4091 3277 Size: 794 20 4947 Fragments arranged by size: 4947 794 20 ClaI AT'CG_AT Cuts at: 1554 2673 1554 Size: 1119 4642 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726 Size: 54 1425 50 275 18 1378 889 1629 Cuts at: 5726 8 Size: 43 Fragments arranged by size: 1629 1425 1378 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2214 2286 2576 3691 1353 Size: 861 72 290 1115 3423 Fragments arranged by size: 3423 1115 861 290 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2574 2998 2574 Size: 424 5337 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2421 2560 2625 3074 3824 4421 Size: 393 566 223 139 65 449 750 597 Cuts at: 4421 5208 5476 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 750 597 566 449 393 268 223 139 65 EcoO109I rG'GnC_Cy Cuts at: 2110 2121 2369 2500 2746 2797 2110 Size: 11 248 131 246 51 5074 Fragments arranged by size: 5074 248 246 131 51 11 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2328 4908 1678 Size: 650 2580 2531 Fragments arranged by size: 2580 2531 650 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2585 2617 4402 4434 4476 Size: 32 135 32 1013 32 1785 32 42 Cuts at: 4476 4508 1373 Size: 32 2626 Fragments arranged by size: 2626 1785 1013 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 2381 3086 2381 Size: 705 5056 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3939 Fragments arranged by size: 3939 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3719 4078 4237 518 Size: 648 2553 359 159 2042 Fragments arranged by size: 2553 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3177 3854 5002 5247 1606 Size: 1571 677 1148 245 2120 Fragments arranged by size: 2120 1571 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2821 3062 3119 4664 1132 Size: 97 1592 241 57 1545 2229 Fragments arranged by size: 2229 1592 1545 241 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 2817 4660 2817 Size: 1843 3918 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PfoI T'CCnGG_A Cuts at: 2576 2576 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2177 2209 3830 3862 5343 5375 2177 Size: 32 1621 32 1481 32 2563 Fragments arranged by size: 2563 1621 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2369 2746 2797 2369 Size: 377 51 5333 Fragments arranged by size: 5333 377 51 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PspOMI G'GGCC_C Cuts at: 2086 2110 2086 Size: 24 5737 PstI C_TGCA'G Cuts at: 830 2601 3113 830 Size: 1771 512 3478 Fragments arranged by size: 3478 1771 512 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 SacI G_AGCT'C Cuts at: 2576 3000 2576 Size: 424 5337 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SapI GCTCTTCn'nnn_ Cuts at: 2576 2576 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SfcI C'TryA_G Cuts at: 826 1045 2278 2597 3109 4244 4925 5116 826 Size: 219 1233 319 512 1135 681 191 1471 Fragments arranged by size: 1471 1233 1135 681 512 319 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 2085 2115 2717 3088 1872 Size: 213 30 602 371 4545 Fragments arranged by size: 4545 602 371 213 30 SmlI C'TyrA_G Cuts at: 820 1017 1943 2517 3865 4766 5028 820 Size: 197 926 574 1348 901 262 1553 Fragments arranged by size: 1553 1348 926 901 574 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 2881 3200 513 Size: 552 939 877 319 3074 Fragments arranged by size: 3074 939 877 552 319 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3788 3805 3958 4143 4588 325 Size: 1255 2208 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2820 4007 5719 Size: 274 80 33 51 1443 843 1187 1712 Cuts at: 5719 96 Size: 138 Fragments arranged by size: 1712 1443 1187 843 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 2083 2113 2715 3086 1870 Size: 213 30 602 371 4545 Fragments arranged by size: 4545 602 371 213 30 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI