(Circular) (Six-base) MAPSORT of: pFN21AB6425.seq Check: 8952 from: 1 to: 5635 pFN21AB6425 5635 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2141 2141 Size: 5635 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3445 278 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2978 1937 Size: 1041 4594 Acc65I G'GTAC_C Cuts at: 2956 2956 Size: 5635 AclI AA'CG_TT Cuts at: 1563 3762 4135 1563 Size: 2199 373 3063 Fragments arranged by size: 3063 2199 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2118 3698 5082 5350 1632 Size: 486 1580 1384 268 1917 Fragments arranged by size: 1917 1580 1384 486 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5438 AflIII A'CryG_T Cuts at: 1203 4534 5511 1203 Size: 3331 977 1327 Fragments arranged by size: 3331 1327 977 AgeI A'CCGG_T Cuts at: 4466 4466 Size: 5635 AhdI GACnn_n'nnGTC Cuts at: 4364 4364 Size: 5635 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5635 AlwNI CAG_nnn'CTG Cuts at: 2215 3378 4950 2215 Size: 1163 1572 2900 Fragments arranged by size: 2900 1572 1163 ApaI G_GGCC'C Cuts at: 2301 2301 Size: 5635 ApaLI G'TGCA_C Cuts at: 3692 4848 3692 Size: 1156 4479 ApoI r'AATT_y Cuts at: 1493 2924 2944 3170 3322 5431 1493 Size: 1431 20 226 152 2109 1697 Fragments arranged by size: 2109 1697 1431 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 4189 160 Size: 872 3157 1606 Fragments arranged by size: 3157 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 AvaI C'yCGr_G Cuts at: 1870 1943 2352 2650 2960 1870 Size: 73 409 298 310 4545 Fragments arranged by size: 4545 409 310 298 73 BamHI G'GATC_C Cuts at: 2965 3332 2965 Size: 367 5268 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2956 4411 618 Size: 325 217 627 1169 1455 1842 Fragments arranged by size: 1842 1455 1169 627 325 217 BanII G_rGCy'C Cuts at: 1391 2301 2448 2621 2796 2954 1391 Size: 910 147 173 175 158 4072 Fragments arranged by size: 4072 910 175 173 158 147 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4664 BbvCI CC'TCA_GC Cuts at: 2580 2580 Size: 5635 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2558 2592 3826 3860 1903 Size: 34 621 34 1234 34 3678 Fragments arranged by size: 3678 1234 621 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3527 4737 3527 Size: 1210 4425 BclI T'GATC_A Cuts at: 1548 1548 Size: 5635 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2435 2726 4246 Size: 107 122 71 631 605 763 291 1520 Cuts at: 4246 136 Size: 1525 Fragments arranged by size: 1525 1520 763 631 605 291 122 107 71 BglII A'GATC_T Cuts at: 5630 5630 Size: 5635 BlpI GC'TnA_GC Cuts at: 2048 3052 2048 Size: 1004 4631 Bme1580I G_kGCm'C Cuts at: 1790 2301 3696 4852 1790 Size: 511 1395 1156 2573 Fragments arranged by size: 2573 1395 1156 511 BmgBI CAC'GTC Cuts at: 1318 2232 1318 Size: 914 4721 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2699 3366 4324 Size: 416 53 83 399 24 1282 667 958 Cuts at: 4324 442 Size: 1753 Fragments arranged by size: 1753 1282 958 667 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5635 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2220 2310 2578 4295 1239 Size: 981 90 268 1717 2579 Fragments arranged by size: 2579 1717 981 268 90 Bpu10I CC'TnA_GC Cuts at: 2143 2580 2143 Size: 437 5198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3760 4625 4923 3760 Size: 865 298 4472 Fragments arranged by size: 4472 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2378 2486 4298 882 Size: 617 879 108 1812 2219 Fragments arranged by size: 2219 1812 879 617 108 BsaAI yAC'GTr Cuts at: 493 1204 3426 493 Size: 711 2222 2702 Fragments arranged by size: 2702 2222 711 BsaBI GATnn'nnATC Cuts at: 5629 5629 Size: 5635 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3442 3824 4459 275 Size: 53 83 186 936 1909 382 635 1451 Fragments arranged by size: 1909 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4068 4466 4740 4887 5298 4068 Size: 398 274 147 411 4405 Fragments arranged by size: 4405 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 2301 2331 2724 Size: 30 165 30 717 30 151 30 393 Cuts at: 2724 2754 2772 2802 1178 Size: 30 18 30 4011 Fragments arranged by size: 4011 717 393 165 151 30 30 30 30 30 30 18 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2885 1186 Size: 1699 3936 BseYI C'CCAG_C Cuts at: 2513 2605 2761 4838 5334 2513 Size: 92 156 2077 496 2814 Fragments arranged by size: 2814 2077 496 156 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2169 1163 Size: 573 433 4629 Fragments arranged by size: 4629 573 433 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3085 3846 4874 1540 Size: 275 186 1084 761 1028 2301 Fragments arranged by size: 2301 1084 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3696 3781 4852 3696 Size: 85 1071 4479 Fragments arranged by size: 4479 1071 85 BsmI GAATG_Cn' Cuts at: 3146 3239 3146 Size: 93 5542 BsmBI CGTCTCn'nnnn_ Cuts at: 2238 2238 Size: 5635 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2448 2621 2796 2954 3696 3781 Size: 399 511 147 173 175 158 742 85 Cuts at: 3781 4852 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 511 399 175 173 158 147 85 BspEI T'CCGG_A Cuts at: 5298 5298 Size: 5635 BspHI T'CATG_A Cuts at: 3524 5254 3524 Size: 1730 3905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2141 2990 844 Size: 1297 849 3489 Fragments arranged by size: 3489 1297 849 BsrBI CCG'CTC Cuts at: 1394 1823 3522 1394 Size: 429 1699 3507 Fragments arranged by size: 3507 1699 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2772 4130 4304 65 Size: 1183 1524 1358 174 1396 Fragments arranged by size: 1524 1396 1358 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2246 2284 4279 4466 1700 Size: 110 249 187 38 1995 187 2869 Fragments arranged by size: 2869 1995 249 187 187 110 38 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5635 BssHII G'CGCG_C Cuts at: 1925 2011 2037 1925 Size: 86 26 5523 Fragments arranged by size: 5523 86 26 BssSI C'ACGA_G Cuts at: 2786 3695 4707 2786 Size: 909 1012 3714 Fragments arranged by size: 3714 1012 909 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5635 BstBI TT'CG_AA Cuts at: 4439 4439 Size: 5635 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5635 BstXI CCAn_nnnn'nTGG Cuts at: 2251 2251 Size: 5635 BstYI r'GATC_y Cuts at: 1972 2965 3332 3717 3734 5186 5272 5284 5630 Size: 993 367 385 17 1452 86 12 346 Cuts at: 5630 1972 Size: 1977 Fragments arranged by size: 1977 1452 993 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2433 2676 2721 513 Size: 552 1368 243 45 3427 Fragments arranged by size: 3427 1368 552 243 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4896 Fragments arranged by size: 4896 683 56 BtsI GCAGTG_nn' Cuts at: 3151 3945 3965 3151 Size: 794 20 4821 Fragments arranged by size: 4821 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5600 DraI TTT'AAA Cuts at: 2939 2939 Size: 5635 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4642 809 Size: 507 3326 1802 Fragments arranged by size: 3326 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2482 2673 3082 Size: 54 1425 50 275 18 652 191 409 Cuts at: 3082 3971 5600 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 652 409 275 191 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3082 1537 Size: 275 1270 4090 Fragments arranged by size: 4090 1270 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3565 1353 Size: 2212 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4222 4596 4742 387 Size: 1064 138 2633 374 146 1280 Fragments arranged by size: 2633 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2118 2220 2310 2578 3698 4295 5082 Size: 393 486 102 90 268 1120 597 787 Cuts at: 5082 5350 1239 Size: 268 1524 Fragments arranged by size: 1524 1120 787 597 486 393 268 268 102 90 EcoO109I rG'GnC_Cy Cuts at: 2297 2510 2555 2727 2737 2895 2297 Size: 213 45 172 10 158 5037 Fragments arranged by size: 5037 213 172 158 45 10 EcoRI G'AATT_C Cuts at: 2924 2944 3322 2924 Size: 20 378 5237 Fragments arranged by size: 5237 378 20 EcoRV GAT'ATC Cuts at: 2167 2167 Size: 5635 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2543 2543 Size: 5635 FspI TGC'GCA Cuts at: 4141 4141 Size: 5635 HaeII r_GCGC'y Cuts at: 1678 2021 2048 4782 1678 Size: 343 27 2734 2531 Fragments arranged by size: 2734 2531 343 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2392 2424 2576 Size: 32 135 32 548 32 240 32 152 Cuts at: 2576 2608 4276 4308 4350 4382 1373 Size: 32 1668 32 42 32 2626 Fragments arranged by size: 2626 1668 548 240 152 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2979 3225 669 Size: 988 281 1041 246 3079 Fragments arranged by size: 3079 1041 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5635 HpaI GTT'AAC Cuts at: 3225 3225 Size: 5635 KpnI G_GTAC'C Cuts at: 2960 2960 Size: 5635 MfeI C'AATT_G Cuts at: 3234 3234 Size: 5635 MluI A'CGCG_T Cuts at: 5511 5511 Size: 5635 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4749 4933 1305 Size: 3444 184 2007 Fragments arranged by size: 3444 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2484 10 Size: 54 1425 343 652 3161 Fragments arranged by size: 3161 1425 652 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3593 3952 4111 518 Size: 648 2427 359 159 2042 Fragments arranged by size: 2427 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2264 3051 3728 4876 5121 1606 Size: 658 787 677 1148 245 2120 Fragments arranged by size: 2120 1148 787 677 658 245 NaeI GCC'GGC Cuts at: 2061 2286 2061 Size: 225 5410 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5083 NdeI CA'TA_TG Cuts at: 387 387 Size: 5635 NgoMIV G'CCGG_C Cuts at: 2059 2284 2059 Size: 225 5410 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5635 NotI GC'GGCC_GC Cuts at: 3082 3082 Size: 5635 NspI r_CATG'y Cuts at: 1132 1229 2476 2993 4538 1132 Size: 97 1247 517 1545 2229 Fragments arranged by size: 2229 1545 1247 517 97 PasI CC'CwG_GG Cuts at: 1348 2379 1348 Size: 1031 4604 PciI A'CATG_T Cuts at: 4534 4534 Size: 5635 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5635 PfoI T'CCnGG_A Cuts at: 2742 2742 Size: 5635 PmeI GTTT'AAAC Cuts at: 2939 2939 Size: 5635 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3736 5217 5249 3704 Size: 32 1481 32 4090 Fragments arranged by size: 4090 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2510 2555 2510 Size: 45 5590 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5635 PsiI TTA'TAA Cuts at: 3205 3205 Size: 5635 PspOMI G'GGCC_C Cuts at: 2297 2297 Size: 5635 PstI C_TGCA'G Cuts at: 830 2058 2509 2987 830 Size: 1228 451 478 3478 Fragments arranged by size: 3478 1228 478 451 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5635 PvuII CAG'CTG Cuts at: 2264 2264 Size: 5635 SalI G'TCGA_C Cuts at: 1936 2977 1936 Size: 1041 4594 SbfI CC_TGCA'GG Cuts at: 2509 2987 2509 Size: 478 5157 ScaI AGT'ACT Cuts at: 3883 5595 3883 Size: 1712 3923 SfcI C'TryA_G Cuts at: 826 1045 2054 2259 2316 2505 2667 2983 4118 Size: 219 1009 205 57 189 162 316 1135 Cuts at: 4118 4799 4990 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1009 681 316 219 205 191 189 162 57 SfiI GGCCn_nnn'nGGCC Cuts at: 2726 2726 Size: 5635 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5635 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5635 SmaI CCC'GGG Cuts at: 1872 2354 2962 1872 Size: 482 608 4545 Fragments arranged by size: 4545 608 482 SmlI C'TyrA_G Cuts at: 820 1017 1943 3739 4640 4902 820 Size: 197 926 1796 901 262 1553 Fragments arranged by size: 1796 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3426 493 Size: 2933 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5635 SphI G_CATG'C Cuts at: 1132 2993 1132 Size: 1861 3774 SspI AAT'ATT Cuts at: 5 52 3559 5 Size: 47 3507 2081 Fragments arranged by size: 3507 2081 47 StuI AGG'CCT Cuts at: 1517 2414 2784 1517 Size: 897 370 4368 Fragments arranged by size: 4368 897 370 StyI C'CwwG_G Cuts at: 513 1065 2301 2865 3074 513 Size: 552 1236 564 209 3074 Fragments arranged by size: 3074 1236 564 552 209 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3662 3679 3832 4017 4462 325 Size: 1255 2082 17 153 185 445 1498 Fragments arranged by size: 2082 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3881 5593 96 Size: 274 80 33 51 1443 1904 1712 138 Fragments arranged by size: 1904 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2724 2756 2772 2804 5595 5627 2724 Size: 32 16 32 2791 32 2732 Fragments arranged by size: 2791 2732 32 32 32 16 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5635 XbaI T'CTAG_A Cuts at: 2971 2971 Size: 5635 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2546 2546 Size: 5635 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5635 XmaI C'CCGG_G Cuts at: 1870 2352 2960 1870 Size: 482 608 4545 Fragments arranged by size: 4545 608 482 XmnI GAAnn'nnTTC Cuts at: 3764 3764 Size: 5635 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3443 276 Size: 53 83 186 936 1909 2468 Fragments arranged by size: 2468 1909 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI