Restriction map for pFN21AB6425
 (Circular) (Six-base) MAPSORT of: pFN21AB6425.seq  Check: 8952  from: 1  to: 5635



pFN21AB6425  5635 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:40  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2141    2141 
   Size:       5635
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3445     278 
   Size:         53      83     186     936    1909    2468

  Fragments arranged by size: 

                2468    1909     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2978    1937 
   Size:       1041    4594
 
Acc65I G'GTAC_C

Cuts at:   2956    2956 
   Size:       5635
 
AclI AA'CG_TT

Cuts at:   1563    3762    4135    1563 
   Size:       2199     373    3063

  Fragments arranged by size: 

                3063    2199     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2118    3698    5082    5350    1632 
   Size:        486    1580    1384     268    1917

  Fragments arranged by size: 

                1917    1580    1384     486     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5438
 
AflIII A'CryG_T

Cuts at:   1203    4534    5511    1203 
   Size:       3331     977    1327

  Fragments arranged by size: 

                3331    1327     977
 
AgeI A'CCGG_T

Cuts at:   4466    4466 
   Size:       5635
 
AhdI GACnn_n'nnGTC

Cuts at:   4364    4364 
   Size:       5635
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5635
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5635
 
AlwNI CAG_nnn'CTG

Cuts at:   2215    3378    4950    2215 
   Size:       1163    1572    2900

  Fragments arranged by size: 

                2900    1572    1163
 
ApaI G_GGCC'C

Cuts at:   2301    2301 
   Size:       5635
 
ApaLI G'TGCA_C

Cuts at:   3692    4848    3692 
   Size:       1156    4479
 
ApoI r'AATT_y

Cuts at:   1493    2924    2944    3170    3322    5431    1493 
   Size:       1431      20     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1431     226     152      20
 
AseI AT'TA_AT

Cuts at:    160    1032    4189     160 
   Size:        872    3157    1606

  Fragments arranged by size: 

                3157    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5635
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2352    2650    2960    1870 
   Size:         73     409     298     310    4545

  Fragments arranged by size: 

                4545     409     310     298      73
 
BamHI G'GATC_C

Cuts at:   2965    3332    2965 
   Size:        367    5268
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2956    4411     618 
   Size:        325     217     627    1169    1455    1842

  Fragments arranged by size: 

                1842    1455    1169     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2301    2448    2621    2796    2954    1391 
   Size:        910     147     173     175     158    4072

  Fragments arranged by size: 

                4072     910     175     173     158     147
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4664
 
BbvCI CC'TCA_GC

Cuts at:   2580    2580 
   Size:       5635
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2558    2592    3826    3860    1903 
   Size:         34     621      34    1234      34    3678

  Fragments arranged by size: 

                3678    1234     621      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3527    4737    3527 
   Size:       1210    4425
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5635
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2435    2726    4246 
   Size:        107     122      71     631     605     763     291    1520

Cuts at:   4246     136 
   Size:       1525

  Fragments arranged by size: 

                1525    1520     763     631     605     291     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5630    5630 
   Size:       5635
 
BlpI GC'TnA_GC

Cuts at:   2048    3052    2048 
   Size:       1004    4631
 
Bme1580I G_kGCm'C

Cuts at:   1790    2301    3696    4852    1790 
   Size:        511    1395    1156    2573

  Fragments arranged by size: 

                2573    1395    1156     511
 
BmgBI CAC'GTC

Cuts at:   1318    2232    1318 
   Size:        914    4721
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2699    3366    4324 
   Size:        416      53      83     399      24    1282     667     958

Cuts at:   4324     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1282     958     667     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5635
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2220    2310    2578    4295    1239 
   Size:        981      90     268    1717    2579

  Fragments arranged by size: 

                2579    1717     981     268      90
 
Bpu10I CC'TnA_GC

Cuts at:   2143    2580    2143 
   Size:        437    5198
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3760    4625    4923    3760 
   Size:        865     298    4472

  Fragments arranged by size: 

                4472     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2378    2486    4298     882 
   Size:        617     879     108    1812    2219

  Fragments arranged by size: 

                2219    1812     879     617     108
 
BsaAI yAC'GTr

Cuts at:    493    1204    3426     493 
   Size:        711    2222    2702

  Fragments arranged by size: 

                2702    2222     711
 
BsaBI GATnn'nnATC

Cuts at:   5629    5629 
   Size:       5635
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3442    3824    4459     275 
   Size:         53      83     186     936    1909     382     635    1451

  Fragments arranged by size: 

                1909    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4068    4466    4740    4887    5298    4068 
   Size:        398     274     147     411    4405

  Fragments arranged by size: 

                4405     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2120    2150    2301    2331    2724 
   Size:         30     165      30     717      30     151      30     393

Cuts at:   2724    2754    2772    2802    1178 
   Size:         30      18      30    4011

  Fragments arranged by size: 

                4011     717     393     165     151      30      30      30
                  30      30      30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2885    1186 
   Size:       1699    3936
 
BseYI C'CCAG_C

Cuts at:   2513    2605    2761    4838    5334    2513 
   Size:         92     156    2077     496    2814

  Fragments arranged by size: 

                2814    2077     496     156      92
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2169    1163 
   Size:        573     433    4629

  Fragments arranged by size: 

                4629     573     433
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3085    3846    4874    1540 
   Size:        275     186    1084     761    1028    2301

  Fragments arranged by size: 

                2301    1084    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3696    3781    4852    3696 
   Size:         85    1071    4479

  Fragments arranged by size: 

                4479    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3146    3239    3146 
   Size:         93    5542
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2238    2238 
   Size:       5635
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2301    2448    2621    2796    2954    3696    3781 
   Size:        399     511     147     173     175     158     742      85

Cuts at:   3781    4852    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     511     399     175     173     158
                 147      85
 
BspEI T'CCGG_A

Cuts at:   5298    5298 
   Size:       5635
 
BspHI T'CATG_A

Cuts at:   3524    5254    3524 
   Size:       1730    3905
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2141    2990     844 
   Size:       1297     849    3489

  Fragments arranged by size: 

                3489    1297     849
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3522    1394 
   Size:        429    1699    3507

  Fragments arranged by size: 

                3507    1699     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2772    4130    4304      65 
   Size:       1183    1524    1358     174    1396

  Fragments arranged by size: 

                1524    1396    1358    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2059    2246    2284    4279    4466    1700 
   Size:        110     249     187      38    1995     187    2869

  Fragments arranged by size: 

                2869    1995     249     187     187     110      38
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5635
 
BssHII G'CGCG_C

Cuts at:   1925    2011    2037    1925 
   Size:         86      26    5523

  Fragments arranged by size: 

                5523      86      26
 
BssSI C'ACGA_G

Cuts at:   2786    3695    4707    2786 
   Size:        909    1012    3714

  Fragments arranged by size: 

                3714    1012     909
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5635
 
BstBI TT'CG_AA

Cuts at:   4439    4439 
   Size:       5635
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5635
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2251    2251 
   Size:       5635
 
BstYI r'GATC_y

Cuts at:   1972    2965    3332    3717    3734    5186    5272    5284    5630 
   Size:        993     367     385      17    1452      86      12     346

Cuts at:   5630    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     993     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2433    2676    2721     513 
   Size:        552    1368     243      45    3427

  Fragments arranged by size: 

                3427    1368     552     243      45
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4896

  Fragments arranged by size: 

                4896     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3151    3945    3965    3151 
   Size:        794      20    4821

  Fragments arranged by size: 

                4821     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5635
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5600
 
DraI TTT'AAA

Cuts at:   2939    2939 
   Size:       5635
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4642     809 
   Size:        507    3326    1802

  Fragments arranged by size: 

                3326    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2482    2673    3082 
   Size:         54    1425      50     275      18     652     191     409

Cuts at:   3082    3971    5600       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     652     409     275     191      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3082    1537 
   Size:        275    1270    4090

  Fragments arranged by size: 

                4090    1270     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3565    1353 
   Size:       2212    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4222    4596    4742     387 
   Size:       1064     138    2633     374     146    1280

  Fragments arranged by size: 

                2633    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2118    2220    2310    2578    3698    4295    5082 
   Size:        393     486     102      90     268    1120     597     787

Cuts at:   5082    5350    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1120     787     597     486     393     268     268
                 102      90
 
EcoO109I rG'GnC_Cy

Cuts at:   2297    2510    2555    2727    2737    2895    2297 
   Size:        213      45     172      10     158    5037

  Fragments arranged by size: 

                5037     213     172     158      45      10
 
EcoRI G'AATT_C

Cuts at:   2924    2944    3322    2924 
   Size:         20     378    5237

  Fragments arranged by size: 

                5237     378      20
 
EcoRV GAT'ATC

Cuts at:   2167    2167 
   Size:       5635
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2543    2543 
   Size:       5635
 
FspI TGC'GCA

Cuts at:   4141    4141 
   Size:       5635
 
HaeII r_GCGC'y

Cuts at:   1678    2021    2048    4782    1678 
   Size:        343      27    2734    2531

  Fragments arranged by size: 

                2734    2531     343      27
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2120    2152    2392    2424    2576 
   Size:         32     135      32     548      32     240      32     152

Cuts at:   2576    2608    4276    4308    4350    4382    1373 
   Size:         32    1668      32      42      32    2626

  Fragments arranged by size: 

                2626    1668     548     240     152     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2979    3225     669 
   Size:        988     281    1041     246    3079

  Fragments arranged by size: 

                3079    1041     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5635
 
HpaI GTT'AAC

Cuts at:   3225    3225 
   Size:       5635
 
KpnI G_GTAC'C

Cuts at:   2960    2960 
   Size:       5635
 
MfeI C'AATT_G

Cuts at:   3234    3234 
   Size:       5635
 
MluI A'CGCG_T

Cuts at:   5511    5511 
   Size:       5635
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4749    4933    1305 
   Size:       3444     184    2007

  Fragments arranged by size: 

                3444    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2484      10 
   Size:         54    1425     343     652    3161

  Fragments arranged by size: 

                3161    1425     652     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3593    3952    4111     518 
   Size:        648    2427     359     159    2042

  Fragments arranged by size: 

                2427    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2264    3051    3728    4876    5121    1606 
   Size:        658     787     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     787     677     658     245
 
NaeI GCC'GGC

Cuts at:   2061    2286    2061 
   Size:        225    5410
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    5083
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5635
 
NgoMIV G'CCGG_C

Cuts at:   2059    2284    2059 
   Size:        225    5410
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5635
 
NotI GC'GGCC_GC

Cuts at:   3082    3082 
   Size:       5635
 
NspI r_CATG'y

Cuts at:   1132    1229    2476    2993    4538    1132 
   Size:         97    1247     517    1545    2229

  Fragments arranged by size: 

                2229    1545    1247     517      97
 
PasI CC'CwG_GG

Cuts at:   1348    2379    1348 
   Size:       1031    4604
 
PciI A'CATG_T

Cuts at:   4534    4534 
   Size:       5635
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5635
 
PfoI T'CCnGG_A

Cuts at:   2742    2742 
   Size:       5635
 
PmeI GTTT'AAAC

Cuts at:   2939    2939 
   Size:       5635
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3704    3736    5217    5249    3704 
   Size:         32    1481      32    4090

  Fragments arranged by size: 

                4090    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2510    2555    2510 
   Size:         45    5590
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5635
 
PsiI TTA'TAA

Cuts at:   3205    3205 
   Size:       5635
 
PspOMI G'GGCC_C

Cuts at:   2297    2297 
   Size:       5635
 
PstI C_TGCA'G

Cuts at:    830    2058    2509    2987     830 
   Size:       1228     451     478    3478

  Fragments arranged by size: 

                3478    1228     478     451
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5635
 
PvuII CAG'CTG

Cuts at:   2264    2264 
   Size:       5635
 
SalI G'TCGA_C

Cuts at:   1936    2977    1936 
   Size:       1041    4594
 
SbfI CC_TGCA'GG

Cuts at:   2509    2987    2509 
   Size:        478    5157
 
ScaI AGT'ACT

Cuts at:   3883    5595    3883 
   Size:       1712    3923
 
SfcI C'TryA_G

Cuts at:    826    1045    2054    2259    2316    2505    2667    2983    4118 
   Size:        219    1009     205      57     189     162     316    1135

Cuts at:   4118    4799    4990     826 
   Size:        681     191    1471

  Fragments arranged by size: 

                1471    1135    1009     681     316     219     205     191
                 189     162      57
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2726    2726 
   Size:       5635
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5635
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5635
 
SmaI CCC'GGG

Cuts at:   1872    2354    2962    1872 
   Size:        482     608    4545

  Fragments arranged by size: 

                4545     608     482
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3739    4640    4902     820 
   Size:        197     926    1796     901     262    1553

  Fragments arranged by size: 

                1796    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3426     493 
   Size:       2933    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5635
 
SphI G_CATG'C

Cuts at:   1132    2993    1132 
   Size:       1861    3774
 
SspI AAT'ATT

Cuts at:      5      52    3559       5 
   Size:         47    3507    2081

  Fragments arranged by size: 

                3507    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2414    2784    1517 
   Size:        897     370    4368

  Fragments arranged by size: 

                4368     897     370
 
StyI C'CwwG_G

Cuts at:    513    1065    2301    2865    3074     513 
   Size:        552    1236     564     209    3074

  Fragments arranged by size: 

                3074    1236     564     552     209
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3662    3679    3832    4017    4462     325 
   Size:       1255    2082      17     153     185     445    1498

  Fragments arranged by size: 

                2082    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3881    5593      96 
   Size:        274      80      33      51    1443    1904    1712     138

  Fragments arranged by size: 

                1904    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2724    2756    2772    2804    5595    5627    2724 
   Size:         32      16      32    2791      32    2732

  Fragments arranged by size: 

                2791    2732      32      32      32      16
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5635
 
XbaI T'CTAG_A

Cuts at:   2971    2971 
   Size:       5635
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2546    2546 
   Size:       5635
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5635
 
XmaI C'CCGG_G

Cuts at:   1870    2352    2960    1870 
   Size:        482     608    4545

  Fragments arranged by size: 

                4545     608     482
 
XmnI GAAnn'nnTTC

Cuts at:   3764    3764 
   Size:       5635
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3443     276 
   Size:         53      83     186     936    1909    2468

  Fragments arranged by size: 

                2468    1909     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     AscI    AvrII     BaeI     BbeI    BfrBI     BplI
    BsiWI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI     FseI    FspAI
     KasI     NarI     NruI     NsiI     PacI     PmlI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfoI     SrfI
     SwaI
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