Restriction map for
pFN21AB8856
(Circular) (Six-base) MAPSORT of: pFN21AB8856.seq Check: 3107 from: 1 to: 4975
pFN21AB8856 4975 bp
With 182 enzymes: SgfI *
October 20, 2009 10:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2785 278
Size: 53 83 186 936 1249 2468
Fragments arranged by size:
2468 1249 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2318 1937
Size: 381 4594
Acc65I G'GTAC_C
Cuts at: 2296 2296
Size: 4975
AclI AA'CG_TT
Cuts at: 1563 3102 3475 1563
Size: 1539 373 3063
Fragments arranged by size:
3063 1539 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 3038 4422 4690 1632
Size: 494 912 1384 268 1917
Fragments arranged by size:
1917 1384 912 494 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4778
AflIII A'CryG_T
Cuts at: 1203 3874 4851 1203
Size: 2671 977 1327
Fragments arranged by size:
2671 1327 977
AgeI A'CCGG_T
Cuts at: 3806 3806
Size: 4975
AhdI GACnn_n'nnGTC
Cuts at: 3704 3704
Size: 4975
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4975
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4975
AlwNI CAG_nnn'CTG
Cuts at: 2718 4290 2718
Size: 1572 3403
ApaLI G'TGCA_C
Cuts at: 3032 4188 3032
Size: 1156 3819
ApoI r'AATT_y
Cuts at: 1493 2284 2510 2662 4771 1493
Size: 791 226 152 2109 1697
Fragments arranged by size:
2109 1697 791 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3529 160
Size: 872 2497 1606
Fragments arranged by size:
2497 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4975
AvaI C'yCGr_G
Cuts at: 1870 1943 2300 1870
Size: 73 357 4545
Fragments arranged by size:
4545 357 73
BamHI G'GATC_C
Cuts at: 2305 2672 2305
Size: 367 4608
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2296 3751 618
Size: 325 217 627 509 1455 1842
Fragments arranged by size:
1842 1455 627 509 325 217
BanII G_rGCy'C
Cuts at: 1391 2130 2294 1391
Size: 739 164 4072
Fragments arranged by size:
4072 739 164
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2184 928
Size: 971 285 3719
Fragments arranged by size:
3719 971 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3166 3200 1903
Size: 34 1229 34 3678
Fragments arranged by size:
3678 1229 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2867 4077 2867
Size: 1210 3765
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4975
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3586 136
Size: 107 122 71 631 605 1914 1525
Fragments arranged by size:
1914 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4970 4970
Size: 4975
BlpI GC'TnA_GC
Cuts at: 2392 2392
Size: 4975
Bme1580I G_kGCm'C
Cuts at: 1790 3036 4192 1790
Size: 1246 1156 2573
Fragments arranged by size:
2573 1246 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4975
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2706 3664 442
Size: 416 53 83 399 24 1289 958 1753
Fragments arranged by size:
1753 1289 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4975
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3635 1239
Size: 2396 2579
Bpu10I CC'TnA_GC
Cuts at: 2090 2090
Size: 4975
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 3100 3965 4263 2252
Size: 848 865 298 2964
Fragments arranged by size:
2964 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3638 882
Size: 617 2139 2219
Fragments arranged by size:
2219 2139 617
BsaAI yAC'GTr
Cuts at: 493 1204 2766 493
Size: 711 1562 2702
Fragments arranged by size:
2702 1562 711
BsaBI GATnn'nnATC
Cuts at: 4969 4969
Size: 4975
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2782 3164 3799 275
Size: 53 83 186 936 1249 382 635 1451
Fragments arranged by size:
1451 1249 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2187 3408 3806 4080 4227 4638 2187
Size: 1221 398 274 147 411 2524
Fragments arranged by size:
2524 1221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2045 2075 1178
Size: 30 165 30 642 30 4078
Fragments arranged by size:
4078 642 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 1186
Size: 841 4134
BseYI C'CCAG_C
Cuts at: 4178 4674 4178
Size: 496 4479
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4402
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2425 3186 4214 1540
Size: 275 186 424 761 1028 2301
Fragments arranged by size:
2301 1028 761 424 275 186
BsiHKAI G_wGCw'C
Cuts at: 2130 3036 3121 4192 2130
Size: 906 85 1071 2913
Fragments arranged by size:
2913 1071 906 85
BsmI GAATG_Cn'
Cuts at: 2486 2579 2486
Size: 93 4882
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2130 2294 3036 3121 4192 1391
Size: 399 340 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 340 164 85
BspEI T'CCGG_A
Cuts at: 4638 4638
Size: 4975
BspHI T'CATG_A
Cuts at: 2864 4594 2864
Size: 1730 3245
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2330 844
Size: 1486 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2862 1394
Size: 429 1039 3507
Fragments arranged by size:
3507 1039 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3470 3644 65
Size: 1183 2222 174 1396
Fragments arranged by size:
2222 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3619 3806 1700
Size: 110 1809 187 2869
Fragments arranged by size:
2869 1809 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4975
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4975
BssSI C'ACGA_G
Cuts at: 2039 3035 4047 2039
Size: 996 1012 2967
Fragments arranged by size:
2967 1012 996
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4975
BstBI TT'CG_AA
Cuts at: 3779 3779
Size: 4975
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4975
BstYI r'GATC_y
Cuts at: 1972 2305 2672 3057 3074 4526 4612 4624 4970
Size: 333 367 385 17 1452 86 12 346
Cuts at: 4970 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 333 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3484
Fragments arranged by size:
3484 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4236
Fragments arranged by size:
4236 683 56
BtsI GCAGTG_nn'
Cuts at: 2491 3285 3305 2491
Size: 794 20 4161
Fragments arranged by size:
4161 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4975
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4940
DraI TTT'AAA
Cuts at: 2279 2279
Size: 4975
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3982 809
Size: 507 2666 1802
Fragments arranged by size:
2666 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2422 3311
Size: 54 1425 50 275 18 213 379 889
Cuts at: 3311 4940 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 379 275 213 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2422 1537
Size: 275 610 4090
Fragments arranged by size:
4090 610 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2905 1353
Size: 1552 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3562 3936 4082 387
Size: 1064 138 1973 374 146 1280
Fragments arranged by size:
1973 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2128 2128
Size: 4975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 3038 3635 4422 4690 1239
Size: 393 494 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 597 494 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2021 2021
Size: 4975
EcoO109I rG'GnC_Cy
Cuts at: 2238 2238
Size: 4975
EcoRI G'AATT_C
Cuts at: 2284 2662 2284
Size: 378 4597
FspI TGC'GCA
Cuts at: 3481 3481
Size: 4975
HaeII r_GCGC'y
Cuts at: 1678 4122 1678
Size: 2444 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3616 3648 3690 3722 1373
Size: 32 135 32 2044 32 42 32 2626
Fragments arranged by size:
2626 2044 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2319 2565 669
Size: 988 281 381 246 3079
Fragments arranged by size:
3079 988 381 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4975
HpaI GTT'AAC
Cuts at: 2565 2565
Size: 4975
KpnI G_GTAC'C
Cuts at: 2300 2300
Size: 4975
MfeI C'AATT_G
Cuts at: 2574 2574
Size: 4975
MluI A'CGCG_T
Cuts at: 4851 4851
Size: 4975
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4089 4273 1305
Size: 2784 184 2007
Fragments arranged by size:
2784 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 10
Size: 54 1425 343 213 2940
Fragments arranged by size:
2940 1425 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2933 3292 3451 518
Size: 648 1767 359 159 2042
Fragments arranged by size:
2042 1767 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2391 3068 4216 4461 1606
Size: 785 677 1148 245 2120
Fragments arranged by size:
2120 1148 785 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3484
Fragments arranged by size:
3484 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4975
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4975
NotI GC'GGCC_GC
Cuts at: 2422 2422
Size: 4975
NspI r_CATG'y
Cuts at: 1132 1229 2013 2333 3878 1132
Size: 97 784 320 1545 2229
Fragments arranged by size:
2229 1545 784 320 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4975
PciI A'CATG_T
Cuts at: 3874 3874
Size: 4975
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4975
PfoI T'CCnGG_A
Cuts at: 2214 2214
Size: 4975
PmeI GTTT'AAAC
Cuts at: 2279 2279
Size: 4975
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3044 3076 4557 4589 3044
Size: 32 1481 32 3430
Fragments arranged by size:
3430 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4975
PsiI TTA'TAA
Cuts at: 2545 2545
Size: 4975
PstI C_TGCA'G
Cuts at: 830 2151 2327 830
Size: 1321 176 3478
Fragments arranged by size:
3478 1321 176
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4975
SacI G_AGCT'C
Cuts at: 2130 2130
Size: 4975
SalI G'TCGA_C
Cuts at: 1936 2317 1936
Size: 381 4594
SbfI CC_TGCA'GG
Cuts at: 2327 2327
Size: 4975
ScaI AGT'ACT
Cuts at: 2060 3223 4935 2060
Size: 1163 1712 2100
Fragments arranged by size:
2100 1712 1163
SfcI C'TryA_G
Cuts at: 826 1045 2147 2323 3458 4139 4330 826
Size: 219 1102 176 1135 681 191 1471
Fragments arranged by size:
1471 1135 1102 681 219 191 176
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4975
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4975
SmaI CCC'GGG
Cuts at: 1872 2302 1872
Size: 430 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 3079 3980 4242 820
Size: 197 926 324 812 901 262 1553
Fragments arranged by size:
1553 926 901 812 324 262 197
SnaBI TAC'GTA
Cuts at: 493 2766 493
Size: 2273 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4975
SphI G_CATG'C
Cuts at: 1132 2013 2333 1132
Size: 881 320 3774
Fragments arranged by size:
3774 881 320
SspI AAT'ATT
Cuts at: 5 52 2899 5
Size: 47 2847 2081
Fragments arranged by size:
2847 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4975
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2414 513
Size: 552 939 237 173 3074
Fragments arranged by size:
3074 939 552 237 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3002 3019 3172 3357 3802 325
Size: 1255 1422 17 153 185 445 1498
Fragments arranged by size:
1498 1422 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2058 3221 4933
Size: 274 80 33 51 1443 81 1163 1712
Cuts at: 4933 96
Size: 138
Fragments arranged by size:
1712 1443 1163 274 138 81 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 4935 4967 2045
Size: 32 2858 32 2053
Fragments arranged by size:
2858 2053 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4975
XbaI T'CTAG_A
Cuts at: 2311 2311
Size: 4975
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4975
XmaI C'CCGG_G
Cuts at: 1870 2300 1870
Size: 430 4545
XmnI GAAnn'nnTTC
Cuts at: 3104 3104
Size: 4975
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2783 276
Size: 53 83 186 936 1249 2468
Fragments arranged by size:
2468 1249 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI