(Circular) (Six-base) MAPSORT of: pFN21AE0367.seq Check: 8655 from: 1 to: 6967 pFN21AE0367 6967 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4777 278 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4310 1937 Size: 2373 4594 Acc65I G'GTAC_C Cuts at: 4288 4288 Size: 6967 AclI AA'CG_TT Cuts at: 1563 5094 5467 1563 Size: 3531 373 3063 Fragments arranged by size: 3531 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2020 2492 3053 3571 5030 6414 6682 1632 Size: 388 472 561 518 1459 1384 268 1917 Fragments arranged by size: 1917 1459 1384 561 518 472 388 268 AfeI AGC'GCT Cuts at: 2684 2684 Size: 6967 AflII C'TTAA_G Cuts at: 820 1017 2435 820 Size: 197 1418 5352 Fragments arranged by size: 5352 1418 197 AflIII A'CryG_T Cuts at: 1203 5866 6843 1203 Size: 4663 977 1327 Fragments arranged by size: 4663 1327 977 AgeI A'CCGG_T Cuts at: 5798 5798 Size: 6967 AhdI GACnn_n'nnGTC Cuts at: 2392 5696 2392 Size: 3304 3663 AleI CACnn'nnGTG Cuts at: 1166 2317 1166 Size: 1151 5816 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2307 2778 1184 Size: 1123 471 5373 Fragments arranged by size: 5373 1123 471 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2999 3031 2999 Size: 32 6935 AlwNI CAG_nnn'CTG Cuts at: 4120 4710 6282 4120 Size: 590 1572 4805 Fragments arranged by size: 4805 1572 590 ApaLI G'TGCA_C Cuts at: 5024 6180 5024 Size: 1156 5811 ApoI r'AATT_y Cuts at: 1493 2673 2701 2824 2920 3780 4276 4502 4654 Size: 1180 28 123 96 860 496 226 152 Cuts at: 4654 6763 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1180 860 496 226 152 123 96 28 AseI AT'TA_AT Cuts at: 160 1032 5521 160 Size: 872 4489 1606 Fragments arranged by size: 4489 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 AvaI C'yCGr_G Cuts at: 1870 1943 2184 4292 1870 Size: 73 241 2108 4545 Fragments arranged by size: 4545 2108 241 73 AvrII C'CTAG_G Cuts at: 3787 3787 Size: 6967 BamHI G'GATC_C Cuts at: 4297 4664 4297 Size: 367 6600 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4288 5743 618 Size: 325 217 627 2501 1455 1842 Fragments arranged by size: 2501 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4286 1391 Size: 2895 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2450 2906 3571 928 Size: 971 551 456 665 4324 Fragments arranged by size: 4324 971 665 551 456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5158 5192 1903 Size: 34 3221 34 3678 Fragments arranged by size: 3678 3221 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2346 4859 6069 2346 Size: 2513 1210 3244 Fragments arranged by size: 3244 2513 1210 BclI T'GATC_A Cuts at: 1548 2085 2124 1548 Size: 537 39 6391 Fragments arranged by size: 6391 537 39 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5578 136 Size: 107 122 71 631 605 3906 1525 Fragments arranged by size: 3906 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3323 6962 3323 Size: 3639 3328 BlpI GC'TnA_GC Cuts at: 4384 4384 Size: 6967 Bme1580I G_kGCm'C Cuts at: 1790 2767 5028 6184 1790 Size: 977 2261 1156 2573 Fragments arranged by size: 2573 2261 1156 977 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6967 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2794 4698 5656 Size: 416 53 83 399 24 1377 1904 958 Cuts at: 5656 442 Size: 1753 Fragments arranged by size: 1904 1753 1377 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6967 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4216 4216 Size: 6967 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5627 1239 Size: 4388 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2097 5092 5957 6255 2097 Size: 2995 865 298 2809 Fragments arranged by size: 2995 2809 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5630 882 Size: 617 4131 2219 Fragments arranged by size: 4131 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4758 493 Size: 711 3554 2702 Fragments arranged by size: 3554 2702 711 BsaBI GATnn'nnATC Cuts at: 2658 6961 2658 Size: 4303 2664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4774 5156 5791 275 Size: 53 83 186 936 3241 382 635 1451 Fragments arranged by size: 3241 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5400 5798 6072 6219 6630 5400 Size: 398 274 147 411 5737 Fragments arranged by size: 5737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6742 Fragments arranged by size: 6742 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4189 1186 Size: 3003 3964 BseYI C'CCAG_C Cuts at: 6170 6666 6170 Size: 496 6471 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3503 4106 1163 Size: 573 1767 603 4024 Fragments arranged by size: 4024 1767 603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4417 5178 6206 1540 Size: 275 186 2416 761 1028 2301 Fragments arranged by size: 2416 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5028 5113 6184 5028 Size: 85 1071 5811 Fragments arranged by size: 5811 1071 85 BsmI GAATG_Cn' Cuts at: 4478 4571 4478 Size: 93 6874 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2767 4286 5028 5113 6184 1391 Size: 399 977 1519 742 85 1071 2174 Fragments arranged by size: 2174 1519 1071 977 742 399 85 BspEI T'CCGG_A Cuts at: 6630 6630 Size: 6967 BspHI T'CATG_A Cuts at: 4856 6586 4856 Size: 1730 5237 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3299 4322 844 Size: 2455 1023 3489 Fragments arranged by size: 3489 2455 1023 BsrBI CCG'CTC Cuts at: 1394 1823 4854 1394 Size: 429 3031 3507 Fragments arranged by size: 3507 3031 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5462 5636 65 Size: 1183 4214 174 1396 Fragments arranged by size: 4214 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5611 5798 1700 Size: 110 3801 187 2869 Fragments arranged by size: 3801 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6967 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6967 BssSI C'ACGA_G Cuts at: 5027 6039 5027 Size: 1012 5955 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6967 BstBI TT'CG_AA Cuts at: 5771 5771 Size: 6967 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6967 BstYI r'GATC_y Cuts at: 1972 2935 3323 4297 4664 5049 5066 6518 6604 Size: 963 388 974 367 385 17 1452 86 Cuts at: 6604 6616 6962 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 974 963 388 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6415 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6228 Fragments arranged by size: 6228 683 56 BtsI GCAGTG_nn' Cuts at: 3491 4483 5277 5297 3491 Size: 992 794 20 5161 Fragments arranged by size: 5161 992 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6967 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6932 DraI TTT'AAA Cuts at: 2026 2980 4271 2026 Size: 954 1291 4722 Fragments arranged by size: 4722 1291 954 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2428 5974 809 Size: 507 1112 3546 1802 Fragments arranged by size: 3546 1802 1112 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3507 4414 5303 Size: 54 1425 50 275 18 1677 907 889 Cuts at: 5303 6932 8 Size: 1629 43 Fragments arranged by size: 1677 1629 1425 907 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4414 1537 Size: 275 2602 4090 Fragments arranged by size: 4090 2602 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2229 2751 3892 3995 4207 4897 1353 Size: 686 190 522 1141 103 212 690 3423 Fragments arranged by size: 3423 1141 690 686 522 212 190 103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2243 5554 5928 6074 387 Size: 1064 138 654 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 654 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2492 3053 3571 5030 5627 6414 Size: 393 388 472 561 518 1459 597 787 Cuts at: 6414 6682 1239 Size: 268 1524 Fragments arranged by size: 1524 1459 787 597 561 518 472 393 388 268 EcoO109I rG'GnC_Cy Cuts at: 3235 3235 Size: 6967 EcoRI G'AATT_C Cuts at: 4276 4654 4276 Size: 378 6589 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3478 3478 Size: 6967 FspI TGC'GCA Cuts at: 5473 5473 Size: 6967 HaeII r_GCGC'y Cuts at: 1678 2686 6114 1678 Size: 1008 3428 2531 Fragments arranged by size: 3428 2531 1008 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2378 2410 5608 5640 5682 Size: 32 135 32 806 32 3198 32 42 Cuts at: 5682 5714 1373 Size: 32 2626 Fragments arranged by size: 3198 2626 806 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4051 4311 4557 669 Size: 988 281 2113 260 246 3079 Fragments arranged by size: 3079 2113 988 281 260 246 HindIII A'AGCT_T Cuts at: 748 2990 748 Size: 2242 4725 HpaI GTT'AAC Cuts at: 4051 4557 4051 Size: 506 6461 KpnI G_GTAC'C Cuts at: 4292 4292 Size: 6967 MfeI C'AATT_G Cuts at: 4566 4566 Size: 6967 MluI A'CGCG_T Cuts at: 6843 6843 Size: 6967 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6081 6265 1305 Size: 4776 184 2007 Fragments arranged by size: 4776 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3509 10 Size: 54 1425 343 1677 3468 Fragments arranged by size: 3468 1677 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2199 2289 2317 3116 3317 3641 3818 Size: 648 1033 90 28 799 201 324 177 Cuts at: 3818 4925 5284 5443 518 Size: 1107 359 159 2042 Fragments arranged by size: 2042 1107 1033 799 648 359 324 201 177 159 90 28 MspA1I CmG'CkG Cuts at: 1606 4117 4383 5060 6208 6453 1606 Size: 2511 266 677 1148 245 2120 Fragments arranged by size: 2511 2120 1148 677 266 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6415 NdeI CA'TA_TG Cuts at: 387 387 Size: 6967 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6967 NotI GC'GGCC_GC Cuts at: 4414 4414 Size: 6967 NspI r_CATG'y Cuts at: 1132 1229 4325 5870 1132 Size: 97 3096 1545 2229 Fragments arranged by size: 3096 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6967 PciI A'CATG_T Cuts at: 5866 5866 Size: 6967 PflMI CCAn_nnn'nTGG Cuts at: 1108 3954 4185 1108 Size: 2846 231 3890 Fragments arranged by size: 3890 2846 231 PfoI T'CCnGG_A Cuts at: 2534 4022 2534 Size: 1488 5479 PmeI GTTT'AAAC Cuts at: 4271 4271 Size: 6967 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5036 5068 6549 6581 5036 Size: 32 1481 32 5422 Fragments arranged by size: 5422 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3235 3235 Size: 6967 PshAI GACnn'nnGTC Cuts at: 1613 3620 1613 Size: 2007 4960 PsiI TTA'TAA Cuts at: 4537 4537 Size: 6967 PstI C_TGCA'G Cuts at: 830 4319 830 Size: 3489 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6967 PvuII CAG'CTG Cuts at: 4117 4117 Size: 6967 SalI G'TCGA_C Cuts at: 1936 4309 1936 Size: 2373 4594 SapI GCTCTTCn'nnn_ Cuts at: 2751 2751 Size: 6967 SbfI CC_TGCA'GG Cuts at: 4319 4319 Size: 6967 ScaI AGT'ACT Cuts at: 5215 6927 5215 Size: 1712 5255 SfcI C'TryA_G Cuts at: 826 1045 4315 5450 6131 6322 826 Size: 219 3270 1135 681 191 1471 Fragments arranged by size: 3270 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6967 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6967 SmaI CCC'GGG Cuts at: 1872 4294 1872 Size: 2422 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2112 2435 5071 5972 6234 820 Size: 197 926 169 323 2636 901 262 1553 Fragments arranged by size: 2636 1553 926 901 323 262 197 169 SnaBI TAC'GTA Cuts at: 493 4758 493 Size: 4265 2702 SpeI A'CTAG_T Cuts at: 152 2252 152 Size: 2100 4867 SphI G_CATG'C Cuts at: 1132 4325 1132 Size: 3193 3774 SspI AAT'ATT Cuts at: 5 52 2571 2616 3264 4891 5 Size: 47 2519 45 648 1627 2081 Fragments arranged by size: 2519 2081 1627 648 47 45 StuI AGG'CCT Cuts at: 1517 1517 Size: 6967 StyI C'CwwG_G Cuts at: 513 1065 3787 4406 513 Size: 552 2722 619 3074 Fragments arranged by size: 3074 2722 619 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2776 3386 4994 5011 5164 5349 5794 Size: 1255 1196 610 1608 17 153 185 445 Cuts at: 5794 325 Size: 1498 Fragments arranged by size: 1608 1498 1255 1196 610 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5213 6925 96 Size: 274 80 33 51 1443 3236 1712 138 Fragments arranged by size: 3236 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3344 3376 3888 3920 6927 6959 3344 Size: 32 512 32 3007 32 3352 Fragments arranged by size: 3352 3007 512 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6967 XbaI T'CTAG_A Cuts at: 2650 3076 4303 2650 Size: 426 1227 5314 Fragments arranged by size: 5314 1227 426 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6967 XmaI C'CCGG_G Cuts at: 1870 4292 1870 Size: 2422 4545 XmnI GAAnn'nnTTC Cuts at: 2745 5096 2745 Size: 2351 4616 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4775 276 Size: 53 83 186 936 3241 2468 Fragments arranged by size: 3241 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI