(Circular) (Six-base) MAPSORT of: pFN21AE0539.seq Check: 263 from: 1 to: 5914 pFN21AE0539 5914 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2661 3724 278 Size: 53 83 186 936 1125 1063 2468 Fragments arranged by size: 2468 1125 1063 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2184 3257 1937 Size: 247 1073 4594 Fragments arranged by size: 4594 1073 247 Acc65I G'GTAC_C Cuts at: 2617 3235 2617 Size: 618 5296 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2437 3200 3977 5361 5629 1632 Size: 805 763 777 1384 268 1917 Fragments arranged by size: 1917 1384 805 777 763 268 AflII C'TTAA_G Cuts at: 820 1017 3098 820 Size: 197 2081 3636 Fragments arranged by size: 3636 2081 197 AflIII A'CryG_T Cuts at: 1203 4813 5790 1203 Size: 3610 977 1327 Fragments arranged by size: 3610 1327 977 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 4643 4643 Size: 5914 AleI CACnn'nnGTG Cuts at: 1166 2334 2973 1166 Size: 1168 639 4107 Fragments arranged by size: 4107 1168 639 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3071 1184 Size: 1887 4027 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2897 2929 2897 Size: 32 5882 AlwNI CAG_nnn'CTG Cuts at: 3657 5229 3657 Size: 1572 4342 ApaI G_GGCC'C Cuts at: 2596 2596 Size: 5914 ApaLI G'TGCA_C Cuts at: 3971 5127 3971 Size: 1156 4758 ApoI r'AATT_y Cuts at: 1493 3223 3449 3601 5710 1493 Size: 1730 226 152 2109 1697 Fragments arranged by size: 2109 1730 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 2292 3239 1870 Size: 73 349 947 4545 Fragments arranged by size: 4545 947 349 73 BamHI G'GATC_C Cuts at: 2195 3244 3611 2195 Size: 1049 367 4498 Fragments arranged by size: 4498 1049 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2617 3235 4690 618 Size: 325 217 627 830 618 1455 1842 Fragments arranged by size: 1842 1455 830 627 618 325 217 BanII G_rGCy'C Cuts at: 1391 2596 3082 3233 1391 Size: 1205 486 151 4072 Fragments arranged by size: 4072 1205 486 151 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4943 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2765 2799 4105 4139 1903 Size: 34 828 34 1306 34 3678 Fragments arranged by size: 3678 1306 828 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2141 3806 5016 2141 Size: 1665 1210 3039 Fragments arranged by size: 3039 1665 1210 BclI T'GATC_A Cuts at: 1548 2898 1548 Size: 1350 4564 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4525 136 Size: 107 122 71 631 605 2853 1525 Fragments arranged by size: 2853 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5909 5909 Size: 5914 BlpI GC'TnA_GC Cuts at: 2325 3331 2325 Size: 1006 4908 Bme1580I G_kGCm'C Cuts at: 1790 2596 3975 5131 1790 Size: 806 1379 1156 2573 Fragments arranged by size: 2573 1379 1156 806 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2599 3645 4603 Size: 416 53 83 399 24 1182 1046 958 Cuts at: 4603 442 Size: 1753 Fragments arranged by size: 1753 1182 1046 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2462 3104 4574 1239 Size: 1223 642 1470 2579 Fragments arranged by size: 2579 1470 1223 642 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2627 4039 4904 5202 2627 Size: 1412 865 298 3339 Fragments arranged by size: 3339 1412 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4577 882 Size: 617 3078 2219 Fragments arranged by size: 3078 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2281 3705 493 Size: 711 1077 1424 2702 Fragments arranged by size: 2702 1424 1077 711 BsaBI GATnn'nnATC Cuts at: 2549 5908 2549 Size: 3359 2555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2658 3721 4103 4738 Size: 53 83 186 936 1125 1063 382 635 Cuts at: 4738 275 Size: 1451 Fragments arranged by size: 1451 1125 1063 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2301 4347 4745 5019 5166 5577 2301 Size: 2046 398 274 147 411 2638 Fragments arranged by size: 2638 2046 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2556 2586 2897 2927 3101 Size: 30 165 30 1153 30 311 30 174 Cuts at: 3101 3131 1178 Size: 30 3961 Fragments arranged by size: 3961 1153 311 174 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3167 1186 Size: 1981 3933 BseYI C'CCAG_C Cuts at: 2810 5117 5613 2810 Size: 2307 496 3111 Fragments arranged by size: 3111 2307 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3083 1163 Size: 573 1347 3994 Fragments arranged by size: 3994 1347 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3364 4125 5153 1540 Size: 275 186 1363 761 1028 2301 Fragments arranged by size: 2301 1363 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2331 3039 3975 4060 5131 2331 Size: 708 936 85 1071 3114 Fragments arranged by size: 3114 1071 936 708 85 BsmI GAATG_Cn' Cuts at: 2348 3164 3425 3518 2348 Size: 816 261 93 4744 Fragments arranged by size: 4744 816 261 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2596 3039 3082 3233 3975 4060 Size: 399 541 265 443 43 151 742 85 Cuts at: 4060 5131 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 541 443 399 265 151 85 43 BspEI T'CCGG_A Cuts at: 2301 5577 2301 Size: 3276 2638 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2605 3269 844 Size: 1761 664 3489 Fragments arranged by size: 3489 1761 664 BsrBI CCG'CTC Cuts at: 1394 1823 2027 3801 1394 Size: 429 204 1774 3507 Fragments arranged by size: 3507 1774 429 204 BsrDI GCAATG_nn' Cuts at: 65 1248 2490 4409 4583 65 Size: 1183 1242 1919 174 1396 Fragments arranged by size: 1919 1396 1242 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2122 4558 4745 1700 Size: 110 312 2436 187 2869 Fragments arranged by size: 2869 2436 312 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5914 BssSI C'ACGA_G Cuts at: 2784 3974 4986 2784 Size: 1190 1012 3712 Fragments arranged by size: 3712 1190 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5914 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 2668 2695 1184 Size: 1484 27 4403 Fragments arranged by size: 4403 1484 27 BstXI CCAn_nnnn'nTGG Cuts at: 2636 2636 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2195 2550 3244 3611 3996 4013 5465 5551 Size: 223 355 694 367 385 17 1452 86 Cuts at: 5551 5563 5909 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 694 385 367 355 346 223 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2103 2103 Size: 5914 BtgI C'CryG_G Cuts at: 513 1065 2253 3130 3147 513 Size: 552 1188 877 17 3280 Fragments arranged by size: 3280 1188 877 552 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5175 Fragments arranged by size: 5175 683 56 BtsI GCAGTG_nn' Cuts at: 3430 4224 4244 3430 Size: 794 20 5100 Fragments arranged by size: 5100 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 3218 3218 Size: 5914 DraIII CAC_nnn'GTG Cuts at: 2173 2173 Size: 5914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4921 809 Size: 507 3605 1802 Fragments arranged by size: 3605 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2574 2866 3127 Size: 54 1425 50 275 18 744 292 261 Cuts at: 3127 3133 3361 4250 5879 8 Size: 6 228 889 1629 43 Fragments arranged by size: 1629 1425 889 744 292 275 261 228 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3361 1537 Size: 275 1549 4090 Fragments arranged by size: 4090 1549 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 3844 1353 Size: 734 1757 3423 Fragments arranged by size: 3423 1757 734 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4501 4875 5021 387 Size: 1064 138 2912 374 146 1280 Fragments arranged by size: 2912 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2437 2462 3104 3200 3977 4574 5361 Size: 393 805 25 642 96 777 597 787 Cuts at: 5361 5629 1239 Size: 268 1524 Fragments arranged by size: 1524 805 787 777 642 597 393 268 96 25 EcoNI CCTnn'n_nnAGG Cuts at: 2750 2882 2750 Size: 132 5782 EcoO109I rG'GnC_Cy Cuts at: 2040 2040 Size: 5914 EcoRI G'AATT_C Cuts at: 3223 3601 3223 Size: 378 5536 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2335 2407 2335 Size: 72 5842 FspI TGC'GCA Cuts at: 2750 4420 2750 Size: 1670 4244 HaeII r_GCGC'y Cuts at: 1678 5061 1678 Size: 3383 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 3101 3133 4555 Size: 32 135 32 527 32 970 32 1422 Cuts at: 4555 4587 4629 4661 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1422 970 527 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2185 3258 3504 669 Size: 988 281 247 1073 246 3079 Fragments arranged by size: 3079 1073 988 281 247 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KpnI G_GTAC'C Cuts at: 2621 3239 2621 Size: 618 5296 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5028 5212 1305 Size: 3723 184 2007 Fragments arranged by size: 3723 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2868 3129 10 Size: 54 1425 343 1036 261 2795 Fragments arranged by size: 2795 1425 1036 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2334 2504 2666 2973 3872 4231 4390 Size: 648 1168 170 162 307 899 359 159 Cuts at: 4390 518 Size: 2042 Fragments arranged by size: 2042 1168 899 648 359 307 170 162 159 MspA1I CmG'CkG Cuts at: 1606 2440 3330 4007 5155 5400 1606 Size: 834 890 677 1148 245 2120 Fragments arranged by size: 2120 1148 890 834 677 245 NcoI C'CATG_G Cuts at: 513 1065 2253 3130 3147 513 Size: 552 1188 877 17 3280 Fragments arranged by size: 3280 1188 877 552 17 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 3272 4817 1132 Size: 97 2043 1545 2229 Fragments arranged by size: 2229 2043 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5914 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 2545 2706 3148 1108 Size: 1437 161 442 3874 Fragments arranged by size: 3874 1437 442 161 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2897 2929 3983 4015 5496 5528 2897 Size: 32 1054 32 1481 32 3283 Fragments arranged by size: 3283 1481 1054 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2040 2040 Size: 5914 PshAI GACnn'nnGTC Cuts at: 1613 2038 1613 Size: 425 5489 PsiI TTA'TAA Cuts at: 2859 3484 2859 Size: 625 5289 PspOMI G'GGCC_C Cuts at: 2592 2592 Size: 5914 PspXI vC'TCGA_Gb Cuts at: 2292 2292 Size: 5914 PstI C_TGCA'G Cuts at: 830 3266 830 Size: 2436 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2440 2440 Size: 5914 SalI G'TCGA_C Cuts at: 1936 2183 3256 1936 Size: 247 1073 4594 Fragments arranged by size: 4594 1073 247 SanDI GG'GwC_CC Cuts at: 2040 2040 Size: 5914 SapI GCTCTTCn'nnn_ Cuts at: 2087 2087 Size: 5914 SbfI CC_TGCA'GG Cuts at: 3266 3266 Size: 5914 ScaI AGT'ACT Cuts at: 2402 2471 4162 5874 2402 Size: 69 1691 1712 2442 Fragments arranged by size: 2442 1712 1691 69 SfcI C'TryA_G Cuts at: 826 1045 3262 4397 5078 5269 826 Size: 219 2217 1135 681 191 1471 Fragments arranged by size: 2217 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 3241 1872 Size: 1369 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2292 2606 3098 4018 4919 5181 Size: 197 926 349 314 492 920 901 262 Cuts at: 5181 820 Size: 1553 Fragments arranged by size: 1553 926 920 901 492 349 314 262 197 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 3272 1132 Size: 2140 3774 SspI AAT'ATT Cuts at: 5 52 3838 5 Size: 47 3786 2081 Fragments arranged by size: 3786 2081 47 StuI AGG'CCT Cuts at: 1517 2298 2390 2745 1517 Size: 781 92 355 4686 Fragments arranged by size: 4686 781 355 92 StyI C'CwwG_G Cuts at: 513 1065 2199 2253 2673 3130 3147 3353 513 Size: 552 1134 54 420 457 17 206 3074 Fragments arranged by size: 3074 1134 552 457 420 206 54 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2266 2516 2945 3204 3941 3958 4111 Size: 1255 686 250 429 259 737 17 153 Cuts at: 4111 4296 4741 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 737 686 445 429 259 250 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 2469 4160 Size: 274 80 33 51 1443 423 69 1691 Cuts at: 4160 5872 96 Size: 1712 138 Fragments arranged by size: 1712 1691 1443 423 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2187 2219 2556 2588 5874 5906 2187 Size: 32 337 32 3286 32 2195 Fragments arranged by size: 3286 2195 337 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2993 1316 Size: 1677 4237 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2260 2260 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 2292 1943 Size: 349 5565 XmaI C'CCGG_G Cuts at: 1870 3239 1870 Size: 1369 4545 XmnI GAAnn'nnTTC Cuts at: 2096 2450 3164 4043 2096 Size: 354 714 879 3967 Fragments arranged by size: 3967 879 714 354 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2659 3722 276 Size: 53 83 186 936 1125 1063 2468 Fragments arranged by size: 2468 1125 1063 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI