Restriction map for
pFN21AB6807
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflIII AgeI
AhdI AlwNI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbsI BbvCI
BciVI BclI BcnI BfaI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FokI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MaeIII MfeI MluI MroI MspA1I
MunI MvnI NarI NciI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tsp509I TspRI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 270 CCGC CCGC(-3/-1)
AfaI 449 GTAC GT^AC
AflII 94 CTTAAG C^TTAAG
Alw44I 197 GTGCAC G^TGCAC
AocI 117 CCTGAGG CC^T[ACGT]AGG
ApaLI 197 GTGCAC G^TGCAC
AvaII 105 GGACC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanII 343 GGGCTC G[GA]GC[CT]^C
BbrPI 200 CACGTG CAC^GTG
BcgI 279 CGATCGACTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 94 CTTAAG C^TTAAG
BsaAI 200 CACGTG [CT]AC^GT[GA]
BsaBI 83 GATACGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 279 CGATCG CG[GA][CT]^CG
BsmFI 36 GTCCC GGGAC(10/14)
BsrDI 294 CATTGC GCAATG(2/0)
BsrFI 356 GCCGGC [GA]^CCGG[CT]
BsrI 33 CCAGT ACTGG(1/-1)
BssSI 323 CTCGTG CACGAG(-5/-1)
BstYI 251 AGATCC [GA]^GATC[CT]
Bsu36I 117 CCTGAGG CC^T[ACGT]AGG
CfoI 129 GCGC GCG^C
Cfr10I 356 GCCGGC [GA]^CCGG[CT]
Eco81I 117 CCTGAGG CC^T[ACGT]AGG
HapII 357 CCGG C^CGG
HhaI 129 GCGC GCG^C
HinP1I 129 GCGC G^CGC
HindIII 307 AAGCTT A^AGCTT
HinfI 88 GAATC G^A[ACGT]TC
HpaII 357 CCGG C^CGG
MamI 83 GATACGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 165 GAAGA GAAGA(8/7)
MflI 251 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 49 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 357 CCGG C^CGG
NaeI 356 GCCGGC GCC^GGC
NgoMIV 356 GCCGGC G^CCGGC
NspI 125 ACATGC [GA]CATG^[CT]
PmaCI 200 CACGTG CAC^GTG
PmlI 200 CACGTG CAC^GTG
PstI 410 CTGCAG CTGCA^G
PvuI 279 CGATCG CGAT^CG
RsaI 449 GTAC GT^AC
SfaNI 168 GATGC GCATC(5/9)
SfcI 410 CTGCAG C^T[GA][CT]AG
SmlI 94 CTTAAG C^T[CT][GA]AG
TfiI 88 GAATC G^A[AT]TC
XcmI 300 CCACCCCAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 251 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 252 GATCC GGATC(4/5)
390 GGATC
AspHI 197 GTGCAC G[AT]GC[AT]^C
320 GTGCTC
BbvI 412 GCAGC GCAGC(8/12)
409 GCTGC
BsiHKAI 197 GTGCAC G[AT]GC[AT]^C
320 GTGCTC
BssKI 405 CCAGG ^CC[ACGT]GG
453 CCAGG
BstNI 405 CCAGG CC^[AT]GG
453 CCAGG
EaeI 2 TGGCCA [CT]^GGCC[GA]
358 CGGCCA
EcoRII 405 CCAGG ^CC[AT]GG
453 CCAGG
MaeII 201 ACGT A^CGT
447 ACGT
MseI 95 TTAA T^TAA
242 TTAA
MvaI 405 CCAGG CC^[AT]GG
453 CCAGG
NlaIII 126 CATG CATG^
192 CATG
ScrFI 405 CCAGG CC^[ACGT]GG
453 CCAGG
TaqI 278 TCGA T^CGA
282 TCGA
Tru9I 95 TTAA T^TAA
242 TTAA
TseI 409 GCTGC G^C[AT]GC
412 GCAGC
TthHB8I 278 TCGA T^CGA
282 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI BmyI BsaJI BsiYI BslI
Bsp1286I Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoT14I Fnu4HI HaeIII
ItaI MboI MnlI MwoI NdeII
NlaIV Sau3AI Sau96I StyI