Restriction map for
pFN21AB6879
##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaII AviII AvrII
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstF5I
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeII MamI MfeI MluI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI
NspI PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp509I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 312 TCCGGA T^CCGGA
AlwNI 157 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 27 GGGCCC GGGCC^C
AspI 458 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 101 CTCGGG C^[CT]CG[GA]G
BamHI 264 GGATCC G^GATCC
BanII 27 GGGCCC G[GA]GC[CT]^C
BmyI 27 GGGCCC G[AGT]GC[ACT]^C
BsaI 303 GAGACC GGTCTC(1/5)
BsaWI 312 TCCGGA [AT]^CCGG[AT]
BseAI 312 TCCGGA T^CCGGA
BsoBI 101 CTCGGG C^[CT]CG[GA]G
Bsp1286I 27 GGGCCC G[AGT]GC[ACT]^C
BspEI 312 TCCGGA T^CCGGA
BsrBI 49 GAGCGG CCGCTC(-3/-3)
BsrI 193 CCAGT ACTGG(1/-1)
BstBI 73 TTCGAA TT^CGAA
BstEII 463 GGTCACC G^GT[ACGT]ACC
BstPI 463 GGTCACC G^GT[ACGT]ACC
BstXI 333 CCAGCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 65 GCGC GCG^C
DdeI 532 CTCAG C^T[ACGT]AG
EarI 493 CTCTTC CTCTTC(1/4)
EcoO65I 463 GGTCACC G^GT[ACGT]ACC
HhaI 65 GCGC GCG^C
HinP1I 65 GCGC G^CGC
Ksp632I 493 CTCTTC CTCTTC(1/4)
MaeIII 464 GTCAC ^GT[ACGT]AC
MroI 312 TCCGGA T^CCGGA
MseI 274 TTAA T^TAA
NruI 45 TCGCGA TCG^CGA
NspV 73 TTCGAA TT^CGAA
PflFI 458 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 477 CAGCTG CAG^CTG
SfuI 73 TTCGAA TT^CGAA
TaqI 74 TCGA T^CGA
Tru9I 274 TTAA T^TAA
Tsp45I 464 GTCAC ^GT[GC]AC
Tth111I 458 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 74 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 46 CGCG CG^CG
66 CGCG
AfaI 135 GTAC GT^AC
440 GTAC
BalI 126 TGGCCA TGG^CCA
142 TGGCCA
BbsI 149 GTCTTC GAAGAC(2/6)
409 GTCTTC
BcnI 350 CCGGG CC^[GC]GG
435 CCGGG
BglI 174 GCCAGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
290 GCCCCCAGGGC
BpmI 474 CTCCAG CTGGAG(16/14)
163 CTGGAG
BpuAI 149 GTCTTC GAAGAC(2/6)
409 GTCTTC
BseRI 13 GAGGAG GAGGAG(10/8)
16 GAGGAG
BsmFI 456 GGGAC GGGAC(10/14)
229 GTCCC
BstNI 261 CCAGG CC^[AT]GG
294 CCAGG
BstUI 46 CGCG CG^CG
66 CGCG
BstYI 264 GGATCC [GA]^GATC[CT]
450 GGATCT
DraII 26 GGGGCCC [GA]G^G[ACGT]CC[CT]
27 GGGCCCC
EcoO109I 26 GGGGCCC [GA]G^G[ACGT]CC[CT]
27 GGGCCCC
EcoRII 261 CCAGG ^CC[AT]GG
294 CCAGG
HinfI 329 GATTC G^A[ACGT]TC
487 GATTC
MflI 264 GGATCC [GA]^GATC[CT]
450 GGATCT
MluNI 126 TGGCCA TGG^CCA
142 TGGCCA
MscI 126 TGGCCA TGG^CCA
142 TGGCCA
MspA1I 8 CAGCGG C[AC]G^C[GT]G
477 CAGCTG
MvaI 261 CCAGG CC^[AT]GG
294 CCAGG
MvnI 46 CGCG CG^CG
66 CGCG
NciI 350 CCGGG CC^[GC]GG
435 CCGGG
NlaIII 146 CATG CATG^
421 CATG
RsaI 135 GTAC GT^AC
440 GTAC
TfiI 329 GATTC G^A[AT]TC
487 GATTC
TspRI 117 CAGTG CA[GC]TG[ACGT][ACGT]^
157 CAGTG
XcmI 360 CCACTTCTCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
469 CCAGCCTCCAGCTGG
XhoII 264 GGATCC [GA]^GATC[CT]
450 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsiYI BslI BsmAI BssKI Cac8I
Cfr13I DpnII EaeI FauI Fnu4HI
HaeIII HapII HpaII HphI ItaI
MboI MboII MnlI MspI MwoI
NdeII NlaIV Sau3AI Sau96I ScrFI
TseI