Restriction map for
pFN21AB7844
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiEI BsiWI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT14I FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MluI
MluNI MroI MscI MvnI NaeI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI StyI XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AciI 789 GCGG CCGC(-3/-1)
AcyI 1543 GGCGCC G[GA]^CG[CT]C
AlwNI 901 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1078 GGGCCC GGGCC^C
AvaII 1639 GGACC G^G[AT]CC
BanII 1078 GGGCCC G[GA]GC[CT]^C
BbeI 1543 GGCGCC GGCGC^C
BbvI 98 GCAGC GCAGC(8/12)
BciVI 1229 GTATCC GTATCC(6/5)
BglI 1108 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1543 GGCGCC G[GA]^CG[CT]C
BsaWI 7 TCCGGT [AT]^CCGG[AT]
BsgI 1610 GTGCAG GTGCAG(16/14)
BsmBI 254 GAGACG CGTCTC(1/5)
Bsp1407I 705 TGTACA T^GTACA
BsrGI 705 TGTACA T^GTACA
BsrI 1529 CCAGT ACTGG(1/-1)
BstYI 1594 GGATCT [GA]^GATC[CT]
CfoI 1544 GCGC GCG^C
DraI 1266 TTTAAA TTT^AAA
EcoRV 833 GATATC GAT^ATC
EcoT22I 1583 ATGCAT ATGCA^T
Fnu4HI 98 GCAGC GC^[ACGT]GC
HaeII 1543 GGCGCC [GA]GCGC^[CT]
HapII 8 CCGG C^CGG
HgaI 1324 GCGTC GACGC(5/10)
HhaI 1544 GCGC GCG^C
Hin1I 1543 GGCGCC G[GA]^CG[CT]C
HinP1I 1544 GCGC G^CGC
HpaII 8 CCGG C^CGG
ItaI 98 GCAGC GC^[ACGT]GC
KasI 1543 GGCGCC G^GCGCC
MaeII 920 ACGT A^CGT
MflI 1594 GGATCT [GA]^GATC[CT]
MslI 223 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 406 CAGCTG C[AC]G^C[GT]G
MspI 8 CCGG C^CGG
NarI 1543 GGCGCC GG^CGCC
NdeI 1742 CATATG CA^TATG
NsiI 1583 ATGCAT ATGCA^T
NspI 1370 ACATGC [GA]CATG^[CT]
PvuII 406 CAGCTG CAG^CTG
SapI 496 GCTCTTC GCTCTTC(1/4)
ScaI 1651 AGTACT AGT^ACT
SexAI 945 ACCTGGT A^CC[AT]GGT
SfoI 1543 GGCGCC GGC^GCC
SspBI 705 TGTACA T^GTACA
SspI 609 AATATT AAT^ATT
SwaI 1265 ATTTAAAT ATTT^AAAT
TseI 98 GCAGC G^C[AT]GC
XhoII 1594 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 706 GTAC GT^AC
1652 GTAC
Asp700I 607 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1747 GAATTTTTTC
AspHI 112 GAGCAC G[AT]GC[AT]^C
536 GTGCTC
AspI 944 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACATAGTC
BanI 1116 GGCACC G^G[CT][GA]CC
1543 GGCGCC
BsiHKAI 112 GAGCAC G[AT]GC[AT]^C
536 GTGCTC
BsmFI 1638 GGGAC GGGAC(10/14)
394 GTCCC
BsmI 59 GAATGC GAATGC(1/-1)
101 GCATTC
BspHI 228 TCATGA T^CATGA
1008 TCATGA
BstF5I 223 CATCC GGATG(2/0)
1328 CATCC
EarI 497 CTCTTC CTCTTC(1/4)
1524 CTCTTC
FokI 223 CATCC GGATG(9/13)
1328 CATCC
HphI 441 GGTGA GGTGA(8/7)
1072 TCACC
Ksp632I 497 CTCTTC CTCTTC(1/4)
1524 CTCTTC
MfeI 962 CAATTG C^AATTG
1723 CAATTG
MunI 962 CAATTG C^AATTG
1723 CAATTG
PflFI 944 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACATAGTC
PflMI 306 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
940 CCAAGACCTGG
PleI 1732 GACTC GAGTC(4/5)
1633 GAGTC
PstI 249 CTGCAG CTGCA^G
548 CTGCAG
RcaI 228 TCATGA T^CATGA
1008 TCATGA
RsaI 706 GTAC GT^AC
1652 GTAC
SmlI 1086 CTCAAG C^T[CT][GA]AG
1500 CTTGAG
TaqI 321 TCGA T^CGA
514 TCGA
Tth111I 944 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1306 GACATAGTC
TthHB8I 321 TCGA T^CGA
514 TCGA
Van91I 306 CCAAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
940 CCAAGACCTGG
XmnI 607 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1747 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AluI AlwI ApoI BmyI
BsaI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DpnII EcoRII HaeIII
HinfI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I Tsp45I Tsp509I
TspRI