Restriction map for pFN21AB7844
 (Circular) (Six-base) MAPSORT of: pFN21AB7844.seq  Check: 4313  from: 1  to: 6466



pFN21AB7844  6466 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:55  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4276     278 
   Size:         53      83     186     936    2740    2468

  Fragments arranged by size: 

                2740    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3809    1937 
   Size:       1872    4594
 
Acc65I G'GTAC_C

Cuts at:   3787    3787 
   Size:       6466
 
AclI AA'CG_TT

Cuts at:   1563    4593    4966    1563 
   Size:       3030     373    3063

  Fragments arranged by size: 

                3063    3030     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3592    4529    5913    6181    1632 
   Size:       1960     937    1384     268    1917

  Fragments arranged by size: 

                1960    1917    1384     937     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6269
 
AflIII A'CryG_T

Cuts at:   1203    5365    6342    1203 
   Size:       4162     977    1327

  Fragments arranged by size: 

                4162    1327     977
 
AgeI A'CCGG_T

Cuts at:   5297    5297 
   Size:       6466
 
AhdI GACnn_n'nnGTC

Cuts at:   5195    5195 
   Size:       6466
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6466
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6466
 
AlwNI CAG_nnn'CTG

Cuts at:   2911    4209    5781    2911 
   Size:       1298    1572    3596

  Fragments arranged by size: 

                3596    1572    1298
 
ApaI G_GGCC'C

Cuts at:   3087    3087 
   Size:       6466
 
ApaLI G'TGCA_C

Cuts at:   4523    5679    4523 
   Size:       1156    5310
 
ApoI r'AATT_y

Cuts at:   1493    2279    2314    3752    3775    4001    4153    6262    1493 
   Size:        786      35    1438      23     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1438     786     226     152      35      23
            
 
AseI AT'TA_AT

Cuts at:    160    1032    5020     160 
   Size:        872    3988    1606

  Fragments arranged by size: 

                3988    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6466
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3791    1870 
   Size:         73    1848    4545

  Fragments arranged by size: 

                4545    1848      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2068    2101    2068 
   Size:         33    6433
 
BamHI G'GATC_C

Cuts at:   3796    4163    3796 
   Size:        367    6099
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3121    3548    3787    5242     618 
   Size:        325     217     627    1334     427     239    1455    1842

  Fragments arranged by size: 

                1842    1455    1334     627     427     325     239     217
            
 
BanII G_rGCy'C

Cuts at:   1391    3087    3785    1391 
   Size:       1696     698    4072

  Fragments arranged by size: 

                4072    1696     698
 
BbeI G_GCGC'C

Cuts at:   3552    3552 
   Size:       6466
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5495
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4657    4691    1903 
   Size:         34    2720      34    3678

  Fragments arranged by size: 

                3678    2720      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3245    4358    5568    3245 
   Size:       1113    1210    4143

  Fragments arranged by size: 

                4143    1210    1113
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6466
 
BfrBI ATG'CAT

Cuts at:   3590    3590 
   Size:       6466
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3119    5077     136 
   Size:        107     122      71     631     605    1447    1958    1525

  Fragments arranged by size: 

                1958    1525    1447     631     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   6461    6461 
   Size:       6466
 
BlpI GC'TnA_GC

Cuts at:   3883    3883 
   Size:       6466
 
Bme1580I G_kGCm'C

Cuts at:   1790    3087    4527    5683    1790 
   Size:       1297    1440    1156    2573

  Fragments arranged by size: 

                2573    1440    1297    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6466
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4197    5155     442 
   Size:        416      53      83     399      24    2780     958    1753

  Fragments arranged by size: 

                2780    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6466
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    5126    1239 
   Size:       3887    2579
 
Bpu10I CC'TnA_GC

Cuts at:   3172    3172 
   Size:       6466
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3076    3526    4591    5456    5754    3076 
   Size:        450    1065     865     298    3788

  Fragments arranged by size: 

                3788    1065     865     450     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2791    2935    2960    5129     882 
   Size:        617    1292     144      25    2169    2219

  Fragments arranged by size: 

                2219    2169    1292     617     144      25
 
BsaAI yAC'GTr

Cuts at:    493    1204    4257     493 
   Size:        711    3053    2702

  Fragments arranged by size: 

                3053    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6460    6460 
   Size:       6466
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3549    4273    4655    5290 
   Size:         53      83     186     936    2016     724     382     635

Cuts at:   5290     275 
   Size:       1451

  Fragments arranged by size: 

                2016    1451     936     724     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2012    4899    5297    5571    5718    6129    2012 
   Size:       2887     398     274     147     411    2349

  Fragments arranged by size: 

                2887    2349     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2940    2970    1178 
   Size:         30     165      30    1537      30    4674

  Fragments arranged by size: 

                4674    1537     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2616    2619    2948    1186 
   Size:       1430       3     329    4704

  Fragments arranged by size: 

                4704    1430     329       3
 
BseYI C'CCAG_C

Cuts at:   5669    6165    5669 
   Size:        496    5970
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3636    1163 
   Size:        573    1900    3993

  Fragments arranged by size: 

                3993    1900     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3916    4677    5705    1540 
   Size:        275     186    1915     761    1028    2301

  Fragments arranged by size: 

                2301    1915    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2121    2545    4527    4612    5683    2121 
   Size:        424    1982      85    1071    2904

  Fragments arranged by size: 

                2904    1982    1071     424      85
 
BsmI GAATG_Cn'

Cuts at:   2070    2106    3977    4070    2070 
   Size:         36    1871      93    4466

  Fragments arranged by size: 

                4466    1871      93      36
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2253    2253 
   Size:       6466
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2121    2545    3087    3785    4527    4612    5683 
   Size:        399     331     424     542     698     742      85    1071

Cuts at:   5683    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     698     542     424     399     331
                  85
 
BspEI T'CCGG_A

Cuts at:   6129    6129 
   Size:       6466
 
BspHI T'CATG_A

Cuts at:   2233    3013    4355    6085    2233 
   Size:        780    1342    1730    2614

  Fragments arranged by size: 

                2614    1730    1342     780
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3821     844 
   Size:       2977    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4353    1394 
   Size:        429    2530    3507

  Fragments arranged by size: 

                3507    2530     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4961    5135      65 
   Size:       1183    3713     174    1396

  Fragments arranged by size: 

                3713    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5110    5297    1700 
   Size:        110    3300     187    2869

  Fragments arranged by size: 

                3300    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2710      96 
   Size:       2614    3852
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6466
 
BssSI C'ACGA_G

Cuts at:   4526    5538    4526 
   Size:       1012    5454
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6466
 
BstBI TT'CG_AA

Cuts at:   5270    5270 
   Size:       6466
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6466
 
BstYI r'GATC_y

Cuts at:   1972    3599    3796    4163    4548    4565    6017    6103    6115 
   Size:       1627     197     367     385      17    1452      86      12

Cuts at:   6115    6461    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1627    1452     385     367     346     197      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4975

  Fragments arranged by size: 

                4975     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5727

  Fragments arranged by size: 

                5727     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3421    3982    4776    4796    3421 
   Size:        561     794      20    5091

  Fragments arranged by size: 

                5091     794     561      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6466
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2569    2604     529 
   Size:         35    2005      35    4391

  Fragments arranged by size: 

                4391    2005      35      35
 
DraI TTT'AAA

Cuts at:   3273    3770    3273 
   Size:        497    5969
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5473     809 
   Size:        507    4157    1802

  Fragments arranged by size: 

                4157    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3913    4802    6431 
   Size:         54    1425      50     275      18    2083     889    1629

Cuts at:   6431       8 
   Size:         43

  Fragments arranged by size: 

                2083    1629    1425     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3913    1537 
   Size:        275    2101    4090

  Fragments arranged by size: 

                4090    2101     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2508    3535    4396    1353 
   Size:       1155    1027     861    3423

  Fragments arranged by size: 

                3423    1155    1027     861
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5053    5427    5573     387 
   Size:       1064     138    3464     374     146    1280

  Fragments arranged by size: 

                3464    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3592    4529    5126    5913    6181    1239 
   Size:        393    1960     937     597     787     268    1524

  Fragments arranged by size: 

                1960    1524     937     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   3775    4153    3775 
   Size:        378    6088
 
EcoRV GAT'ATC

Cuts at:   2840    2840 
   Size:       6466
 
FspI TGC'GCA

Cuts at:   4972    4972 
   Size:       6466
 
HaeII r_GCGC'y

Cuts at:   1678    3552    5613    1678 
   Size:       1874    2061    2531

  Fragments arranged by size: 

                2531    2061    1874
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2485    2517    2940    2972    3424 
   Size:         32     135      32     913      32     423      32     452

Cuts at:   3424    3445    3456    3477    5107    5139    5181    5213    1373 
   Size:         21      11      21    1630      32      42      32    2626

  Fragments arranged by size: 

                2626    1630     913     452     423     135      42      32
                  32      32      32      32      32      21      21      11
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3810    4056     669 
   Size:        988     281    1872     246    3079

  Fragments arranged by size: 

                3079    1872     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6466
 
HpaI GTT'AAC

Cuts at:   4056    4056 
   Size:       6466
 
KasI G'GCGC_C

Cuts at:   3548    3548 
   Size:       6466
 
KpnI G_GTAC'C

Cuts at:   3791    3791 
   Size:       6466
 
MfeI C'AATT_G

Cuts at:   2967    3728    4065    2967 
   Size:        761     337    5368

  Fragments arranged by size: 

                5368     761     337
 
MluI A'CGCG_T

Cuts at:   6342    6342 
   Size:       6466
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2224    3298    5580    5764    1305 
   Size:        919    1074    2282     184    2007

  Fragments arranged by size: 

                2282    2007    1074     919     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4644

  Fragments arranged by size: 

                4644    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2232    4424    4783    4942     518 
   Size:        648    1066    2192     359     159    2042

  Fragments arranged by size: 

                2192    2042    1066     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2413    3882    4559    5707    5952    1606 
   Size:        807    1469     677    1148     245    2120

  Fragments arranged by size: 

                2120    1469    1148     807     677     245
 
NarI GG'CG_CC

Cuts at:   3549    3549 
   Size:       6466
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4975

  Fragments arranged by size: 

                4975     939     552
 
NdeI CA'TA_TG

Cuts at:    387    3748     387 
   Size:       3361    3105
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6466
 
NotI GC'GGCC_GC

Cuts at:   3913    3913 
   Size:       6466
 
NsiI A_TGCA'T

Cuts at:   3592    3592 
   Size:       6466
 
NspI r_CATG'y

Cuts at:   1132    1229    3379    3824    5369    1132 
   Size:         97    2150     445    1545    2229

  Fragments arranged by size: 

                2229    2150    1545     445      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6466
 
PciI A'CATG_T

Cuts at:   5365    5365 
   Size:       6466
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2317    2951    1108 
   Size:       1209     634    4623

  Fragments arranged by size: 

                4623    1209     634
 
PmeI GTTT'AAAC

Cuts at:   3770    3770 
   Size:       6466
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4535    4567    6048    6080    4535 
   Size:         32    1481      32    4921

  Fragments arranged by size: 

                4921    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6466
 
PsiI TTA'TAA

Cuts at:   4036    4036 
   Size:       6466
 
PspOMI G'GGCC_C

Cuts at:   3083    3083 
   Size:       6466
 
PstI C_TGCA'G

Cuts at:    830    2258    2557    3818     830 
   Size:       1428     299    1261    3478

  Fragments arranged by size: 

                3478    1428    1261     299
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6466
 
PvuII CAG'CTG

Cuts at:   2413    2413 
   Size:       6466
 
SalI G'TCGA_C

Cuts at:   1936    3808    1936 
   Size:       1872    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2508    2508 
   Size:       6466
 
SbfI CC_TGCA'GG

Cuts at:   3818    3818 
   Size:       6466
 
ScaI AGT'ACT

Cuts at:   3658    4714    6426    3658 
   Size:       1056    1712    3698

  Fragments arranged by size: 

                3698    1712    1056
 
SexAI A'CCwGG_T

Cuts at:   2950    2950 
   Size:       6466
 
SfcI C'TryA_G

Cuts at:    826    1045    2254    2553    3039    3814    4949    5630    5821 
   Size:        219    1209     299     486     775    1135     681     191

Cuts at:   5821     826 
   Size:       1471

  Fragments arranged by size: 

                1471    1209    1135     775     681     486     299     219
                 191
 
SfoI GGC'GCC

Cuts at:   3550    3550 
   Size:       6466
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6466
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6466
 
SmaI CCC'GGG

Cuts at:   1872    3793    1872 
   Size:       1921    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3091    3505    4570    5471    5733     820 
   Size:        197     926    1148     414    1065     901     262    1553

  Fragments arranged by size: 

                1553    1148    1065     926     901     414     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4257     493 
   Size:       3764    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6466
 
SphI G_CATG'C

Cuts at:   1132    3824    1132 
   Size:       2692    3774
 
SspI AAT'ATT

Cuts at:      5      52    2616    4390       5 
   Size:         47    2564    1774    2081

  Fragments arranged by size: 

                2564    2081    1774      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6466
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3905     513 
   Size:        552     939    1901    3074

  Fragments arranged by size: 

                3074    1901     939     552
 
SwaI ATTT'AAAT

Cuts at:   3273    3273 
   Size:       6466
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4493    4510    4663    4848    5293     325 
   Size:       1255    2913      17     153     185     445    1498

  Fragments arranged by size: 

                2913    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2710    3656    4712 
   Size:        274      80      33      51    1443     733     946    1056

Cuts at:   4712    6424      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1056     946     733     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2557    2589    6426    6458    2557 
   Size:         32    3837      32    2565

  Fragments arranged by size: 

                3837    2565      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2952    3314    1316 
   Size:       1636     362    4468

  Fragments arranged by size: 

                4468    1636     362
 
XbaI T'CTAG_A

Cuts at:   3802    3802 
   Size:       6466
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6466
 
XmaI C'CCGG_G

Cuts at:   1870    3791    1870 
   Size:       1921    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2616    3756    4595    2616 
   Size:       1140     839    4487

  Fragments arranged by size: 

                4487    1140     839
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4274     276 
   Size:         53      83     186     936    2740    2468

  Fragments arranged by size: 

                2740    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoRI    EcoRV     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI   PspOMI     PstI     PvuI    PvuII     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII    BbvCI     BplI    BsiWI
    BstXI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I     FalI
     FseI    FspAI     NaeI   NgoMIV     NruI     PacI     PfoI     PmlI
    PpuMI    PspXI     PsrI    RsrII     SacI    SacII    SanDI     SfiI
     SrfI     XcmI
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