(Circular) (Six-base) MAPSORT of: pFN21AB7844.seq Check: 4313 from: 1 to: 6466 pFN21AB7844 6466 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4276 278 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3809 1937 Size: 1872 4594 Acc65I G'GTAC_C Cuts at: 3787 3787 Size: 6466 AclI AA'CG_TT Cuts at: 1563 4593 4966 1563 Size: 3030 373 3063 Fragments arranged by size: 3063 3030 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3592 4529 5913 6181 1632 Size: 1960 937 1384 268 1917 Fragments arranged by size: 1960 1917 1384 937 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6269 AflIII A'CryG_T Cuts at: 1203 5365 6342 1203 Size: 4162 977 1327 Fragments arranged by size: 4162 1327 977 AgeI A'CCGG_T Cuts at: 5297 5297 Size: 6466 AhdI GACnn_n'nnGTC Cuts at: 5195 5195 Size: 6466 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6466 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6466 AlwNI CAG_nnn'CTG Cuts at: 2911 4209 5781 2911 Size: 1298 1572 3596 Fragments arranged by size: 3596 1572 1298 ApaI G_GGCC'C Cuts at: 3087 3087 Size: 6466 ApaLI G'TGCA_C Cuts at: 4523 5679 4523 Size: 1156 5310 ApoI r'AATT_y Cuts at: 1493 2279 2314 3752 3775 4001 4153 6262 1493 Size: 786 35 1438 23 226 152 2109 1697 Fragments arranged by size: 2109 1697 1438 786 226 152 35 23 AseI AT'TA_AT Cuts at: 160 1032 5020 160 Size: 872 3988 1606 Fragments arranged by size: 3988 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 AvaI C'yCGr_G Cuts at: 1870 1943 3791 1870 Size: 73 1848 4545 Fragments arranged by size: 4545 1848 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2068 2101 2068 Size: 33 6433 BamHI G'GATC_C Cuts at: 3796 4163 3796 Size: 367 6099 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3121 3548 3787 5242 618 Size: 325 217 627 1334 427 239 1455 1842 Fragments arranged by size: 1842 1455 1334 627 427 325 239 217 BanII G_rGCy'C Cuts at: 1391 3087 3785 1391 Size: 1696 698 4072 Fragments arranged by size: 4072 1696 698 BbeI G_GCGC'C Cuts at: 3552 3552 Size: 6466 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5495 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4657 4691 1903 Size: 34 2720 34 3678 Fragments arranged by size: 3678 2720 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3245 4358 5568 3245 Size: 1113 1210 4143 Fragments arranged by size: 4143 1210 1113 BclI T'GATC_A Cuts at: 1548 1548 Size: 6466 BfrBI ATG'CAT Cuts at: 3590 3590 Size: 6466 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3119 5077 136 Size: 107 122 71 631 605 1447 1958 1525 Fragments arranged by size: 1958 1525 1447 631 605 122 107 71 BglII A'GATC_T Cuts at: 6461 6461 Size: 6466 BlpI GC'TnA_GC Cuts at: 3883 3883 Size: 6466 Bme1580I G_kGCm'C Cuts at: 1790 3087 4527 5683 1790 Size: 1297 1440 1156 2573 Fragments arranged by size: 2573 1440 1297 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4197 5155 442 Size: 416 53 83 399 24 2780 958 1753 Fragments arranged by size: 2780 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6466 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5126 1239 Size: 3887 2579 Bpu10I CC'TnA_GC Cuts at: 3172 3172 Size: 6466 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3076 3526 4591 5456 5754 3076 Size: 450 1065 865 298 3788 Fragments arranged by size: 3788 1065 865 450 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2791 2935 2960 5129 882 Size: 617 1292 144 25 2169 2219 Fragments arranged by size: 2219 2169 1292 617 144 25 BsaAI yAC'GTr Cuts at: 493 1204 4257 493 Size: 711 3053 2702 Fragments arranged by size: 3053 2702 711 BsaBI GATnn'nnATC Cuts at: 6460 6460 Size: 6466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3549 4273 4655 5290 Size: 53 83 186 936 2016 724 382 635 Cuts at: 5290 275 Size: 1451 Fragments arranged by size: 2016 1451 936 724 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2012 4899 5297 5571 5718 6129 2012 Size: 2887 398 274 147 411 2349 Fragments arranged by size: 2887 2349 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2940 2970 1178 Size: 30 165 30 1537 30 4674 Fragments arranged by size: 4674 1537 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2616 2619 2948 1186 Size: 1430 3 329 4704 Fragments arranged by size: 4704 1430 329 3 BseYI C'CCAG_C Cuts at: 5669 6165 5669 Size: 496 5970 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3636 1163 Size: 573 1900 3993 Fragments arranged by size: 3993 1900 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3916 4677 5705 1540 Size: 275 186 1915 761 1028 2301 Fragments arranged by size: 2301 1915 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2545 4527 4612 5683 2121 Size: 424 1982 85 1071 2904 Fragments arranged by size: 2904 1982 1071 424 85 BsmI GAATG_Cn' Cuts at: 2070 2106 3977 4070 2070 Size: 36 1871 93 4466 Fragments arranged by size: 4466 1871 93 36 BsmBI CGTCTCn'nnnn_ Cuts at: 2253 2253 Size: 6466 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2545 3087 3785 4527 4612 5683 Size: 399 331 424 542 698 742 85 1071 Cuts at: 5683 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 698 542 424 399 331 85 BspEI T'CCGG_A Cuts at: 6129 6129 Size: 6466 BspHI T'CATG_A Cuts at: 2233 3013 4355 6085 2233 Size: 780 1342 1730 2614 Fragments arranged by size: 2614 1730 1342 780 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3821 844 Size: 2977 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4353 1394 Size: 429 2530 3507 Fragments arranged by size: 3507 2530 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4961 5135 65 Size: 1183 3713 174 1396 Fragments arranged by size: 3713 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5110 5297 1700 Size: 110 3300 187 2869 Fragments arranged by size: 3300 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2710 96 Size: 2614 3852 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6466 BssSI C'ACGA_G Cuts at: 4526 5538 4526 Size: 1012 5454 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6466 BstBI TT'CG_AA Cuts at: 5270 5270 Size: 6466 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6466 BstYI r'GATC_y Cuts at: 1972 3599 3796 4163 4548 4565 6017 6103 6115 Size: 1627 197 367 385 17 1452 86 12 Cuts at: 6115 6461 1972 Size: 346 1977 Fragments arranged by size: 1977 1627 1452 385 367 346 197 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4975 Fragments arranged by size: 4975 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5727 Fragments arranged by size: 5727 683 56 BtsI GCAGTG_nn' Cuts at: 3421 3982 4776 4796 3421 Size: 561 794 20 5091 Fragments arranged by size: 5091 794 561 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6466 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2569 2604 529 Size: 35 2005 35 4391 Fragments arranged by size: 4391 2005 35 35 DraI TTT'AAA Cuts at: 3273 3770 3273 Size: 497 5969 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5473 809 Size: 507 4157 1802 Fragments arranged by size: 4157 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3913 4802 6431 Size: 54 1425 50 275 18 2083 889 1629 Cuts at: 6431 8 Size: 43 Fragments arranged by size: 2083 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3913 1537 Size: 275 2101 4090 Fragments arranged by size: 4090 2101 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 3535 4396 1353 Size: 1155 1027 861 3423 Fragments arranged by size: 3423 1155 1027 861 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5053 5427 5573 387 Size: 1064 138 3464 374 146 1280 Fragments arranged by size: 3464 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3592 4529 5126 5913 6181 1239 Size: 393 1960 937 597 787 268 1524 Fragments arranged by size: 1960 1524 937 787 597 393 268 EcoRI G'AATT_C Cuts at: 3775 4153 3775 Size: 378 6088 EcoRV GAT'ATC Cuts at: 2840 2840 Size: 6466 FspI TGC'GCA Cuts at: 4972 4972 Size: 6466 HaeII r_GCGC'y Cuts at: 1678 3552 5613 1678 Size: 1874 2061 2531 Fragments arranged by size: 2531 2061 1874 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2485 2517 2940 2972 3424 Size: 32 135 32 913 32 423 32 452 Cuts at: 3424 3445 3456 3477 5107 5139 5181 5213 1373 Size: 21 11 21 1630 32 42 32 2626 Fragments arranged by size: 2626 1630 913 452 423 135 42 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 3810 4056 669 Size: 988 281 1872 246 3079 Fragments arranged by size: 3079 1872 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6466 HpaI GTT'AAC Cuts at: 4056 4056 Size: 6466 KasI G'GCGC_C Cuts at: 3548 3548 Size: 6466 KpnI G_GTAC'C Cuts at: 3791 3791 Size: 6466 MfeI C'AATT_G Cuts at: 2967 3728 4065 2967 Size: 761 337 5368 Fragments arranged by size: 5368 761 337 MluI A'CGCG_T Cuts at: 6342 6342 Size: 6466 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2224 3298 5580 5764 1305 Size: 919 1074 2282 184 2007 Fragments arranged by size: 2282 2007 1074 919 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4644 Fragments arranged by size: 4644 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2232 4424 4783 4942 518 Size: 648 1066 2192 359 159 2042 Fragments arranged by size: 2192 2042 1066 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2413 3882 4559 5707 5952 1606 Size: 807 1469 677 1148 245 2120 Fragments arranged by size: 2120 1469 1148 807 677 245 NarI GG'CG_CC Cuts at: 3549 3549 Size: 6466 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4975 Fragments arranged by size: 4975 939 552 NdeI CA'TA_TG Cuts at: 387 3748 387 Size: 3361 3105 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6466 NotI GC'GGCC_GC Cuts at: 3913 3913 Size: 6466 NsiI A_TGCA'T Cuts at: 3592 3592 Size: 6466 NspI r_CATG'y Cuts at: 1132 1229 3379 3824 5369 1132 Size: 97 2150 445 1545 2229 Fragments arranged by size: 2229 2150 1545 445 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6466 PciI A'CATG_T Cuts at: 5365 5365 Size: 6466 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 2951 1108 Size: 1209 634 4623 Fragments arranged by size: 4623 1209 634 PmeI GTTT'AAAC Cuts at: 3770 3770 Size: 6466 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4535 4567 6048 6080 4535 Size: 32 1481 32 4921 Fragments arranged by size: 4921 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6466 PsiI TTA'TAA Cuts at: 4036 4036 Size: 6466 PspOMI G'GGCC_C Cuts at: 3083 3083 Size: 6466 PstI C_TGCA'G Cuts at: 830 2258 2557 3818 830 Size: 1428 299 1261 3478 Fragments arranged by size: 3478 1428 1261 299 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6466 PvuII CAG'CTG Cuts at: 2413 2413 Size: 6466 SalI G'TCGA_C Cuts at: 1936 3808 1936 Size: 1872 4594 SapI GCTCTTCn'nnn_ Cuts at: 2508 2508 Size: 6466 SbfI CC_TGCA'GG Cuts at: 3818 3818 Size: 6466 ScaI AGT'ACT Cuts at: 3658 4714 6426 3658 Size: 1056 1712 3698 Fragments arranged by size: 3698 1712 1056 SexAI A'CCwGG_T Cuts at: 2950 2950 Size: 6466 SfcI C'TryA_G Cuts at: 826 1045 2254 2553 3039 3814 4949 5630 5821 Size: 219 1209 299 486 775 1135 681 191 Cuts at: 5821 826 Size: 1471 Fragments arranged by size: 1471 1209 1135 775 681 486 299 219 191 SfoI GGC'GCC Cuts at: 3550 3550 Size: 6466 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6466 SmaI CCC'GGG Cuts at: 1872 3793 1872 Size: 1921 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3091 3505 4570 5471 5733 820 Size: 197 926 1148 414 1065 901 262 1553 Fragments arranged by size: 1553 1148 1065 926 901 414 262 197 SnaBI TAC'GTA Cuts at: 493 4257 493 Size: 3764 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6466 SphI G_CATG'C Cuts at: 1132 3824 1132 Size: 2692 3774 SspI AAT'ATT Cuts at: 5 52 2616 4390 5 Size: 47 2564 1774 2081 Fragments arranged by size: 2564 2081 1774 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6466 StyI C'CwwG_G Cuts at: 513 1065 2004 3905 513 Size: 552 939 1901 3074 Fragments arranged by size: 3074 1901 939 552 SwaI ATTT'AAAT Cuts at: 3273 3273 Size: 6466 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4493 4510 4663 4848 5293 325 Size: 1255 2913 17 153 185 445 1498 Fragments arranged by size: 2913 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2710 3656 4712 Size: 274 80 33 51 1443 733 946 1056 Cuts at: 4712 6424 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1056 946 733 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2557 2589 6426 6458 2557 Size: 32 3837 32 2565 Fragments arranged by size: 3837 2565 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2952 3314 1316 Size: 1636 362 4468 Fragments arranged by size: 4468 1636 362 XbaI T'CTAG_A Cuts at: 3802 3802 Size: 6466 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6466 XmaI C'CCGG_G Cuts at: 1870 3791 1870 Size: 1921 4545 XmnI GAAnn'nnTTC Cuts at: 2616 3756 4595 2616 Size: 1140 839 4487 Fragments arranged by size: 4487 1140 839 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4274 276 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI XcmI