################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HapII HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MslI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccII 48 CGCG CG^CG AcsI 304 GAATTC [GA]^AATT[CT] AcyI 180 GGCGTC G[GA]^CG[CT]C AfaI 203 GTAC GT^AC AluI 171 AGCT AG^CT AlwI 150 GGATC GGATC(4/5) Aor51HI 59 AGCGCT AGC^GCT ApoI 304 GAATTC [GA]^AATT[CT] AvaI 27 CCCGAG C^[CT]CG[GA]G BanI 34 GGCACC G^G[CT][GA]CC BbvI 7 GCAGC GCAGC(8/12) BciVI 21 GGATAC GTATCC(6/5) BglI 91 GCCCTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 319 GGGCAC G[AGT]GC[ACT]^C BsaHI 180 GGCGTC G[GA]^CG[CT]C BsaJI 101 CCTGGG C^C[ACGT][ACGT]GG BsmI 81 GAATGC GAATGC(1/-1) BsoBI 27 CCCGAG C^[CT]CG[GA]G Bsp1286I 319 GGGCAC G[AGT]GC[ACT]^C BsrBI 30 GAGCGG CCGCTC(-3/-3) BstF5I 15 GGATG GGATG(2/0) BstUI 48 CGCG CG^CG BstXI 309 CCAAAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 150 GGATCT [GA]^GATC[CT] Cfr13I 98 GGGCC G^G[ACGT]CC DpnII 151 GATC ^GATC DrdI 272 GACAGGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 196 GAAGAG CTCTTC(1/4) Eco47III 59 AGCGCT AGC^GCT EcoRI 304 GAATTC G^AATTC FauI 49 GCGGG CCCGC(4/6) FokI 15 GGATG GGATG(9/13) HaeII 59 AGCGCT [GA]GCGC^[CT] HaeIII 99 GGCC GG^CC HgaI 181 GCGTC GACGC(5/10) Hin1I 180 GGCGTC G[GA]^CG[CT]C HinfI 218 GAGTC G^A[ACGT]TC Ksp632I 196 GAAGAG CTCTTC(1/4) MaeIII 224 GTAAC ^GT[ACGT]AC MboI 151 GATC ^GATC MboII 196 GAAGA GAAGA(8/7) MflI 150 GGATCT [GA]^GATC[CT] MnlI 93 CCTC CCTC(7/6) MspA1I 42 CAGCGG C[AC]G^C[GT]G MvnI 48 CGCG CG^CG NdeII 151 GATC ^GATC PflMI 141 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 218 GAGTC GAGTC(4/5) PstI 132 CTGCAG CTGCA^G RsaI 203 GTAC GT^AC Sau3AI 151 GATC ^GATC Sau96I 98 GGGCC G^G[ACGT]CC SfaNI 248 GCATC GCATC(5/9) SfcI 132 CTGCAG C^T[GA][CT]AG TseI 7 GCAGC G^C[AT]GC Tsp509I 305 AATT ^AATT TspRI 244 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 141 CCACATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 150 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BsiYI 128 CCATCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 141 CCACATTATGG BslI 128 CCATCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 141 CCACATTATGG BssKI 101 CCTGG ^CC[ACGT]GG 177 CCAGG BstNI 101 CCTGG CC^[AT]GG 177 CCAGG Cac8I 49 GCGGGC GC[ACGT]^[ACGT]GC 257 GCTGGC CfoI 47 GCGC GCG^C 60 GCGC EcoRII 101 CCTGG ^CC[AT]GG 177 CCAGG HhaI 47 GCGC GCG^C 60 GCGC HinP1I 47 GCGC G^CGC 60 GCGC MvaI 101 CCTGG CC^[AT]GG 177 CCAGG NlaIII 212 CATG CATG^ 229 CATG NlaIV 34 GGCACC GG[ACGT]^[ACGT]CC 137 GGAACC ScrFI 101 CCTGG CC^[ACGT]GG 177 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AciI Fnu4HI ItaI MwoI