Restriction map for
pFN21AB6485
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III EcoRI EcoRV FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MseI MunI
MvnI NarI NdeI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 925 GTAGAC GT^[AC][GT]AC
AccIII 491 TCCGGA T^CCGGA
AocI 156 CCTGAGG CC^T[ACGT]AGG
AspI 616 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 884 TGGCCA TGG^CCA
BamHI 497 GGATCC G^GATCC
BanII 550 GAGCTC G[GA]GC[CT]^C
BclI 975 TGATCA T^GATCA
BsaI 286 GGTCTC GGTCTC(1/5)
BsaWI 491 TCCGGA [AT]^CCGG[AT]
BseAI 491 TCCGGA T^CCGGA
BsiEI 864 CGGCCG CG[GA][CT]^CG
BspEI 491 TCCGGA T^CCGGA
BsrFI 866 GCCGGC [GA]^CCGG[CT]
BstEII 738 GGTGACC G^GT[ACGT]ACC
BstPI 738 GGTGACC G^GT[ACGT]ACC
BstYI 497 GGATCC [GA]^GATC[CT]
Bsu36I 156 CCTGAGG CC^T[ACGT]AGG
Cfr10I 866 GCCGGC [GA]^CCGG[CT]
DsaI 375 CCATGG C^C[GA][CT]GG
EagI 864 CGGCCG C^GGCCG
EarI 109 GAAGAG CTCTTC(1/4)
EclXI 864 CGGCCG C^GGCCG
Eco52I 864 CGGCCG C^GGCCG
Eco81I 156 CCTGAGG CC^T[ACGT]AGG
EcoO65I 738 GGTGACC G^GT[ACGT]ACC
EcoT22I 512 ATGCAT ATGCA^T
FbaI 975 TGATCA T^GATCA
HaeII 202 GGCGCT [GA]GCGC^[CT]
HgaI 794 GACGC GACGC(5/10)
Ksp632I 109 GAAGAG CTCTTC(1/4)
MaeII 392 ACGT A^CGT
MflI 497 GGATCC [GA]^GATC[CT]
MluNI 884 TGGCCA TGG^CCA
MroI 491 TCCGGA T^CCGGA
MscI 884 TGGCCA TGG^CCA
NaeI 866 GCCGGC GCC^GGC
NcoI 375 CCATGG C^CATGG
NgoMIV 866 GCCGGC G^CCGGC
NsiI 512 ATGCAT ATGCA^T
NspI 510 GCATGC [GA]CATG^[CT]
PaeR7I 755 CTCGAG C^TCGAG
PflFI 616 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 616 GACTC GAGTC(4/5)
SacI 550 GAGCTC GAGCT^C
SexAI 89 ACCTGGT A^CC[AT]GGT
SmaI 713 CCCGGG CCC^GGG
SmlI 755 CTCGAG C^T[CT][GA]AG
SphI 510 GCATGC GCATG^C
Tsp509I 312 AATT ^AATT
Tth111I 616 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 570 CCAGCAGGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 755 CTCGAG C^TCGAG
XhoII 497 GGATCC [GA]^GATC[CT]
XmaCI 713 CCCGGG C^CCGGG
XmaI 713 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 529 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
590 CAGGAACTG
AspHI 7 GTGCTC G[AT]GC[AT]^C
550 GAGCTC
BbsI 225 GAAGAC GAAGAC(2/6)
702 GAAGAC
BcnI 713 CCCGG CC^[GC]GG
714 CCGGG
BpuAI 225 GAAGAC GAAGAC(2/6)
702 GAAGAC
BsiHKAI 7 GTGCTC G[AT]GC[AT]^C
550 GAGCTC
BspMI 783 ACCTGC ACCTGC(4/8)
573 GCAGGT
CfoI 203 GCGC GCG^C
870 GCGC
EaeI 864 CGGCCG [CT]^GGCC[GA]
884 TGGCCA
EcoNI 33 CCTTACCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
105 CCTGGAAGAGG
EcoT14I 375 CCATGG C^C[AT][AT]GG
720 CCTTGG
HhaI 203 GCGC GCG^C
870 GCGC
HinP1I 203 GCGC G^CGC
870 GCGC
MslI 624 CACTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
726 CATGGCAGTG
NciI 713 CCCGG CC^[GC]GG
714 CCGGG
PflMI 528 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
915 CCAGGAGATGG
PvuII 442 CAGCTG CAG^CTG
685 CAGCTG
StyI 375 CCATGG C^C[AT][AT]GG
720 CCTTGG
TaqI 756 TCGA T^CGA
842 TCGA
TfiI 406 GATTC G^A[AT]TC
825 GATTC
TthHB8I 756 TCGA T^CGA
842 TCGA
Van91I 528 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
915 CCAGGAGATGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BbvI BmyI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI