(Circular) (Six-base) MAPSORT of: pFN21AB6485.seq Check: 1830 from: 1 to: 5689 pFN21AB6485 5689 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2569 2569 Size: 5689 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2931 3032 1937 Size: 994 101 4594 Fragments arranged by size: 4594 994 101 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 2438 2577 2726 3752 5136 5404 1632 Size: 617 189 139 149 1026 1384 268 1917 Fragments arranged by size: 1917 1384 1026 617 268 189 149 139 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 2633 1166 Size: 1467 4222 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2539 2600 3432 5004 2539 Size: 61 832 1572 3224 Fragments arranged by size: 3224 1572 832 61 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2998 3224 3376 5485 1493 Size: 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2697 2718 2760 3014 1870 Size: 73 754 21 42 254 4545 Fragments arranged by size: 4545 754 254 73 42 21 BamHI G'GATC_C Cuts at: 2502 3019 3386 2502 Size: 517 367 4805 Fragments arranged by size: 4805 517 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 2559 3008 1391 Size: 1168 449 4072 Fragments arranged by size: 4072 1168 449 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2237 2714 928 Size: 971 338 477 3903 Fragments arranged by size: 3903 971 477 338 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 2980 1548 Size: 1432 4257 BfrBI ATG'CAT Cuts at: 2519 2519 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2033 2820 3750 4906 1790 Size: 243 787 930 1156 2573 Fragments arranged by size: 2573 1156 930 787 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2322 2429 2827 4349 1239 Size: 1083 107 398 1522 2579 Fragments arranged by size: 2579 1522 1083 398 107 Bpu10I CC'TnA_GC Cuts at: 2015 2015 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3814 4679 4977 3814 Size: 865 298 4526 Fragments arranged by size: 4526 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2297 4352 882 Size: 617 798 2055 2219 Fragments arranged by size: 2219 2055 798 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2496 4122 4520 4794 4941 5352 2496 Size: 1626 398 274 147 411 2833 Fragments arranged by size: 2833 1626 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2432 2489 1186 Size: 874 372 57 4386 Fragments arranged by size: 4386 874 372 57 BseYI C'CCAG_C Cuts at: 2574 2819 4892 5388 2574 Size: 245 2073 496 2875 Fragments arranged by size: 2875 2073 496 245 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2872 3139 3900 4928 1540 Size: 275 186 871 267 761 1028 2301 Fragments arranged by size: 2301 1028 871 761 275 267 186 BsiHKAI G_wGCw'C Cuts at: 2016 2559 3750 3835 4906 2016 Size: 543 1191 85 1071 2799 Fragments arranged by size: 2799 1191 1071 543 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2033 2559 2820 3008 3750 3835 Size: 399 226 17 526 261 188 742 85 Cuts at: 3835 4906 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 526 399 261 226 188 85 17 BspEI T'CCGG_A Cuts at: 2496 5352 2496 Size: 2856 2833 BspHI T'CATG_A Cuts at: 3578 5308 3578 Size: 1730 3959 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2569 2797 3044 844 Size: 1725 228 247 3489 Fragments arranged by size: 3489 1725 247 228 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4184 4358 65 Size: 1183 2936 174 1396 Fragments arranged by size: 2936 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2871 4333 4520 1700 Size: 110 1061 1462 187 2869 Fragments arranged by size: 2869 1462 1061 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 3749 4761 3749 Size: 1012 4677 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 2743 1184 Size: 1559 4130 BstYI r'GATC_y Cuts at: 1972 2502 3019 3386 3771 3788 5240 5326 5338 Size: 530 517 367 385 17 1452 86 12 Cuts at: 5338 5684 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 530 517 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2162 2162 Size: 5689 BtgI C'CryG_G Cuts at: 513 1065 2004 2380 513 Size: 552 939 376 3822 Fragments arranged by size: 3822 939 552 376 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2238 2628 2742 3205 3999 4019 2238 Size: 390 114 463 794 20 3908 Fragments arranged by size: 3908 794 463 390 114 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2242 2277 529 Size: 35 1678 35 3941 Fragments arranged by size: 3941 1678 35 35 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2869 2889 3136 Size: 54 1425 50 275 18 1039 20 247 Cuts at: 3136 4025 5654 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1039 889 275 247 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2869 3136 1537 Size: 275 1057 267 4090 Fragments arranged by size: 4090 1057 275 267 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 3619 1353 Size: 756 1510 3423 Fragments arranged by size: 3423 1510 756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2557 2557 Size: 5689 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2322 2429 2438 2577 2726 2827 Size: 393 617 73 107 9 139 149 101 Cuts at: 2827 3752 4349 5136 5404 1239 Size: 925 597 787 268 1524 Fragments arranged by size: 1524 925 787 617 597 393 268 149 139 107 101 73 9 EcoNI CCTnn'n_nnAGG Cuts at: 2042 2114 2042 Size: 72 5617 EcoO109I rG'GnC_Cy Cuts at: 2149 2722 2940 2949 2149 Size: 573 218 9 4889 Fragments arranged by size: 4889 573 218 9 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2211 4836 1678 Size: 533 2625 2531 Fragments arranged by size: 2625 2531 533 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4330 4362 4404 4436 1373 Size: 32 135 32 2758 32 42 32 2626 Fragments arranged by size: 2758 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2002 4803 4987 1305 Size: 697 2801 184 2007 Fragments arranged by size: 2801 2007 697 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2891 10 Size: 54 1425 343 1059 2808 Fragments arranged by size: 2808 1425 1059 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2633 2735 3647 4006 4165 518 Size: 648 843 624 102 912 359 159 2042 Fragments arranged by size: 2042 912 843 648 624 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2449 2692 2775 3105 3782 4930 5175 1606 Size: 843 243 83 330 677 1148 245 2120 Fragments arranged by size: 2120 1148 843 677 330 245 243 83 NaeI GCC'GGC Cuts at: 2873 2873 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 2004 2380 513 Size: 552 939 376 3822 Fragments arranged by size: 3822 939 552 376 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NgoMIV G'CCGG_C Cuts at: 2871 2871 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NsiI A_TGCA'T Cuts at: 2521 2521 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 2519 3047 4592 1132 Size: 97 1290 528 1545 2229 Fragments arranged by size: 2229 1545 1290 528 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2539 2926 1108 Size: 1431 387 3871 Fragments arranged by size: 3871 1431 387 PfoI T'CCnGG_A Cuts at: 2804 2835 2919 2804 Size: 31 84 5574 Fragments arranged by size: 5574 84 31 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2722 2940 2949 2722 Size: 218 9 5462 Fragments arranged by size: 5462 218 9 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PstI C_TGCA'G Cuts at: 830 2107 2391 2553 3041 830 Size: 1277 284 162 488 3478 Fragments arranged by size: 3478 1277 488 284 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2449 2692 2449 Size: 243 5446 SacI G_AGCT'C Cuts at: 2559 2559 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SexAI A'CCwGG_T Cuts at: 2094 2094 Size: 5689 SfcI C'TryA_G Cuts at: 826 1045 2103 2387 2549 3037 4172 4853 5044 Size: 219 1058 284 162 488 1135 681 191 Cuts at: 5044 826 Size: 1471 Fragments arranged by size: 1471 1135 1058 681 488 284 219 191 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2720 3016 1872 Size: 848 296 4545 Fragments arranged by size: 4545 848 296 SmlI C'TyrA_G Cuts at: 820 1017 1943 2760 3793 4694 4956 820 Size: 197 926 817 1033 901 262 1553 Fragments arranged by size: 1553 1033 926 901 817 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 2519 3047 1132 Size: 1387 528 3774 Fragments arranged by size: 3774 1387 528 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2004 2380 2725 3128 513 Size: 552 939 376 345 403 3074 Fragments arranged by size: 3074 939 552 403 376 345 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2614 3716 3733 3886 4071 4516 325 Size: 1255 1034 1102 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1102 1034 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3935 5647 96 Size: 274 80 33 51 1443 1958 1712 138 Fragments arranged by size: 1958 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5649 5681 5649 Size: 32 5657 Tth111I GACn'n_nGTC Cuts at: 1316 2624 1316 Size: 1308 4381 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2582 2582 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 2760 1943 Size: 817 4872 XmaI C'CCGG_G Cuts at: 1870 2718 3014 1870 Size: 848 296 4545 Fragments arranged by size: 4545 848 296 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI