(Circular) (Six-base) MAPSORT of: pFN21AB6588.seq Check: 4615 from: 1 to: 5923 pFN21AB6588 5923 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2295 2397 3266 1937 Size: 358 102 869 4594 Fragments arranged by size: 4594 869 358 102 Acc65I G'GTAC_C Cuts at: 3244 3244 Size: 5923 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2298 3096 3986 5370 5638 1632 Size: 666 798 890 1384 268 1917 Fragments arranged by size: 1917 1384 890 798 666 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 3198 4822 5799 1203 Size: 1995 1624 977 1327 Fragments arranged by size: 1995 1624 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3114 3146 3114 Size: 32 5891 AlwNI CAG_nnn'CTG Cuts at: 2200 2585 3074 3101 3666 5238 2200 Size: 385 489 27 565 1572 2885 Fragments arranged by size: 2885 1572 565 489 385 27 ApaLI G'TGCA_C Cuts at: 3091 3980 5136 3091 Size: 889 1156 3878 Fragments arranged by size: 3878 1156 889 ApoI r'AATT_y Cuts at: 1493 2285 2669 2951 3232 3458 3610 5719 1493 Size: 792 384 282 281 226 152 2109 1697 Fragments arranged by size: 2109 1697 792 384 282 281 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2604 3021 3248 1870 Size: 73 661 417 227 4545 Fragments arranged by size: 4545 661 417 227 73 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3244 4699 618 Size: 325 217 627 1457 1455 1842 Fragments arranged by size: 1842 1457 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2216 2526 3054 3242 1391 Size: 825 310 528 188 4072 Fragments arranged by size: 4072 825 528 310 188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2597 928 Size: 971 698 4254 Fragments arranged by size: 4254 971 698 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2144 2178 4114 4148 1903 Size: 34 207 34 1936 34 3678 Fragments arranged by size: 3678 1936 207 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4534 136 Size: 107 122 71 631 605 2862 1525 Fragments arranged by size: 2862 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 2547 3048 3340 2547 Size: 501 292 5130 Fragments arranged by size: 5130 501 292 Bme1580I G_kGCm'C Cuts at: 1790 3095 3984 5140 1790 Size: 1305 889 1156 2573 Fragments arranged by size: 2573 1305 1156 889 BmgBI CAC'GTC Cuts at: 1318 2299 1318 Size: 981 4942 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2546 2595 3654 Size: 416 53 83 399 24 1129 49 1059 Cuts at: 3654 4612 442 Size: 958 1753 Fragments arranged by size: 1753 1129 1059 958 416 399 83 53 49 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2230 2230 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2081 2562 2801 2814 2846 4583 1239 Size: 818 24 481 239 13 32 1737 2579 Fragments arranged by size: 2579 1737 818 481 239 32 24 13 Bpu10I CC'TnA_GC Cuts at: 2520 2520 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 2900 5917 2900 Size: 3017 2906 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2130 4356 4754 5028 5175 5586 2130 Size: 2226 398 274 147 411 2467 Fragments arranged by size: 2467 2226 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2201 2761 2984 1186 Size: 868 147 560 223 4125 Fragments arranged by size: 4125 868 560 223 147 BseYI C'CCAG_C Cuts at: 5126 5622 5126 Size: 496 5427 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2357 2831 1163 Size: 573 621 474 4255 Fragments arranged by size: 4255 621 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2526 3054 3095 3984 4069 5140 2526 Size: 528 41 889 85 1071 3309 Fragments arranged by size: 3309 1071 889 528 85 41 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2526 3054 3095 3242 3984 4069 Size: 399 426 310 528 41 147 742 85 Cuts at: 4069 5140 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 528 426 399 310 147 85 41 BspEI T'CCGG_A Cuts at: 2130 5586 2130 Size: 3456 2467 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3278 844 Size: 2434 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2541 4418 4592 65 Size: 1183 1293 1877 174 1396 Fragments arranged by size: 1877 1396 1293 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 2529 4727 2529 Size: 2198 3725 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2379 3253 3620 4005 4022 5474 5560 5572 Size: 407 874 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 874 407 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2241 513 Size: 552 939 237 4195 Fragments arranged by size: 4195 939 552 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2915 3439 4233 4253 2915 Size: 524 794 20 4585 Fragments arranged by size: 4585 794 524 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3370 4259 5888 Size: 54 1425 50 275 18 1540 889 1629 Cuts at: 5888 8 Size: 43 Fragments arranged by size: 1629 1540 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2531 3853 1353 Size: 1178 1322 3423 Fragments arranged by size: 3423 1322 1178 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 4510 4884 5030 387 Size: 1064 138 465 2456 374 146 1280 Fragments arranged by size: 2456 1280 1064 465 374 146 138 EcoICRI GAG'CTC Cuts at: 2524 3052 2524 Size: 528 5395 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2081 2298 2562 2801 2814 2846 Size: 393 425 24 217 264 239 13 32 Cuts at: 2846 3096 3986 4583 5370 5638 1239 Size: 250 890 597 787 268 1524 Fragments arranged by size: 1524 890 787 597 425 393 268 264 250 239 217 32 24 13 EcoRI G'AATT_C Cuts at: 2951 3232 3610 2951 Size: 281 378 5264 Fragments arranged by size: 5264 378 281 EcoRV GAT'ATC Cuts at: 2902 2902 Size: 5923 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2012 5070 1678 Size: 334 3058 2531 Fragments arranged by size: 3058 2531 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2986 3018 3120 3152 4564 Size: 32 135 32 1414 32 102 32 1412 Cuts at: 4564 4596 4638 4670 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1414 1412 135 102 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 3248 3248 Size: 5923 MfeI C'AATT_G Cuts at: 2335 3522 2335 Size: 1187 4736 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3881 4240 4399 518 Size: 648 1578 1137 359 159 2042 Fragments arranged by size: 2042 1578 1137 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2101 2197 3339 4016 5164 5409 1606 Size: 452 43 96 1142 677 1148 245 2120 Fragments arranged by size: 2120 1148 1142 677 452 245 96 43 NcoI C'CATG_G Cuts at: 513 1065 2004 2241 513 Size: 552 939 237 4195 Fragments arranged by size: 4195 939 552 237 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 2729 3109 3202 3281 4826 1132 Size: 97 1500 380 93 79 1545 2229 Fragments arranged by size: 2229 1545 1500 380 97 93 79 PasI CC'CwG_GG Cuts at: 1348 2927 1348 Size: 1579 4344 PciI A'CATG_T Cuts at: 3198 4822 3198 Size: 1624 4299 PflMI CCAn_nnn'nTGG Cuts at: 1108 2585 1108 Size: 1477 4446 PfoI T'CCnGG_A Cuts at: 2904 2904 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 2142 2205 3275 830 Size: 1312 63 1070 3478 Fragments arranged by size: 3478 1312 1070 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2101 2197 2101 Size: 96 5827 SacI G_AGCT'C Cuts at: 2526 3054 2526 Size: 528 5395 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SapI GCTCTTCn'nnn_ Cuts at: 2531 2531 Size: 5923 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 2138 2192 2201 3271 4406 5087 5278 Size: 219 1093 54 9 1070 1135 681 191 Cuts at: 5278 826 Size: 1471 Fragments arranged by size: 1471 1135 1093 1070 681 219 191 54 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2606 3023 3250 1872 Size: 734 417 227 4545 Fragments arranged by size: 4545 734 417 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 4027 4928 5190 820 Size: 197 926 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SrfI GCCC'GGGC Cuts at: 2606 2606 Size: 5923 SspI AAT'ATT Cuts at: 5 52 2072 3847 5 Size: 47 2020 1775 2081 Fragments arranged by size: 2081 2020 1775 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2553 3168 3362 513 Size: 552 939 237 312 615 194 3074 Fragments arranged by size: 3074 939 615 552 312 237 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2347 3950 3967 4120 4305 4750 325 Size: 1255 767 1603 17 153 185 445 1498 Fragments arranged by size: 1603 1498 1255 767 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4169 5881 96 Size: 274 80 33 51 1443 2192 1712 138 Fragments arranged by size: 2192 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2341 2341 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 2604 3021 3248 1870 Size: 734 417 227 4545 Fragments arranged by size: 4545 734 417 227 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SwaI