Restriction map for
pFN21AB6395
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BseRI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AclI 190 AACGTT AA^CGTT
AcsI 154 GAATTC [GA]^AATT[CT]
AcyI 74 GGCGTC G[GA]^CG[CT]C
AlwNI 163 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 154 GAATTC [GA]^AATT[CT]
AspHI 121 GAGCTC G[AT]GC[AT]^C
BalI 392 TGGCCA TGG^CCA
BbvCI 275 CCTCAGC CCTCAGC(-5/-2)
BfaI 10 CTAG C^TAG
BsaAI 553 TACGTG [CT]AC^GT[GA]
BsaHI 74 GGCGTC G[GA]^CG[CT]C
BsiEI 40 CGGCCG CG[GA][CT]^CG
BsiHKAI 121 GAGCTC G[AT]GC[AT]^C
BsmAI 713 GAGAC GTCTC(1/5)
BstF5I 558 GGATG GGATG(2/0)
EagI 40 CGGCCG C^GGCCG
EarI 664 GAAGAG CTCTTC(1/4)
EclXI 40 CGGCCG C^GGCCG
Eco52I 40 CGGCCG C^GGCCG
EcoRI 154 GAATTC G^AATTC
FokI 558 GGATG GGATG(9/13)
HgaI 75 GCGTC GACGC(5/10)
Hin1I 74 GGCGTC G[GA]^CG[CT]C
Ksp632I 664 GAAGAG CTCTTC(1/4)
MaeI 10 CTAG C^TAG
MaeIII 794 GTGAC ^GT[ACGT]AC
MluNI 392 TGGCCA TGG^CCA
MscI 392 TGGCCA TGG^CCA
MseI 326 TTAA T^TAA
MwoI 71 GCGGGCGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 210 CCATGG C^CATGG
PflMI 162 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 190 AACGTT AA^CGTT
PvuII 278 CAGCTG CAG^CTG
SacI 121 GAGCTC GAGCT^C
ScaI 374 AGTACT AGT^ACT
SfcI 96 CTACAG C^T[GA][CT]AG
SmaI 134 CCCGGG CCC^GGG
StuI 668 AGGCCT AGG^CCT
Tru9I 326 TTAA T^TAA
Tsp45I 794 GTGAC ^GT[GC]AC
Tsp509I 155 AATT ^AATT
Van91I 162 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 162 CCAGTCCCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 134 CCCGGG C^CCGGG
XmaI 134 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
BanI 214 GGCACC G^G[CT][GA]CC
343 GGCACC
BanII 23 GAGCCC G[GA]GC[CT]^C
121 GAGCTC
BmyI 23 GAGCCC G[AGT]GC[ACT]^C
121 GAGCTC
BpmI 116 CTCCAG CTGGAG(16/14)
725 CTGGAG
BsgI 802 CTGCAC GTGCAG(16/14)
359 GTGCAG
BsmI 140 GAATGC GAATGC(1/-1)
400 GAATGC
Bsp1286I 23 GAGCCC G[AGT]GC[ACT]^C
121 GAGCTC
BspMI 335 ACCTGC ACCTGC(4/8)
797 ACCTGC
CfoI 4 GCGC GCG^C
384 GCGC
DpnII 113 GATC ^GATC
516 GATC
DraII 283 GGGTCCT [GA]G^G[ACGT]CC[CT]
537 GGGTCCT
DsaI 210 CCATGG C^C[GA][CT]GG
596 CCACGG
EcoO109I 283 GGGTCCT [GA]G^G[ACGT]CC[CT]
537 GGGTCCT
FauI 64 GCGGG CCCGC(4/6)
71 GCGGG
HaeII 3 GGCGCT [GA]GCGC^[CT]
383 AGCGCC
HhaI 4 GCGC GCG^C
384 GCGC
HinP1I 4 GCGC G^CGC
384 GCGC
MboI 113 GATC ^GATC
516 GATC
MspA1I 37 CAGCGG C[AC]G^C[GT]G
278 CAGCTG
NdeII 113 GATC ^GATC
516 GATC
PleI 545 GACTC GAGTC(4/5)
570 GACTC
PpuMI 283 GGGTCCT [GA]G^G[AT]CC[CT]
537 GGGTCCT
Sau3AI 113 GATC ^GATC
516 GATC
TfiI 46 GAATC G^A[AT]TC
265 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BbvI BcnI BsaJI BsiYI BslI
BsmFI BsoBI BsrI BssKI BstNI
Cac8I Cfr13I DdeI EaeI EcoRII
EcoT14I Fnu4HI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MboII
MnlI MspI MvaI NciI NlaIII
NlaIV RsaI Sau96I ScrFI StyI
TseI TspRI