Restriction map for
pFN21AB6385
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BseRI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV FseI FspI HincII HindII
HindIII HpaI MamI MfeI MroI
MseI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PleI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp509I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 383 GGTACC G^GTACC
AccI 424 GTAGAC GT^[AC][GT]AC
AfaI 384 GTAC GT^AC
AgeI 536 ACCGGT A^CCGGT
AlwNI 571 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 383 GGTACC G^GTACC
AspHI 684 GAGCTC G[AT]GC[AT]^C
AspI 145 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 344 TGGCCA TGG^CCA
BbrPI 394 CACGTG CAC^GTG
BbsI 532 GAAGAC GAAGAC(2/6)
BclI 647 TGATCA T^GATCA
BpuAI 532 GAAGAC GAAGAC(2/6)
BsaAI 394 CACGTG [CT]AC^GT[GA]
BsaWI 536 ACCGGT [AT]^CCGG[AT]
BsgI 799 GTGCAG GTGCAG(16/14)
BsiEI 188 CGGTCG CG[GA][CT]^CG
BsiHKAI 684 GAGCTC G[AT]GC[AT]^C
BsrDI 629 GCAATG GCAATG(2/0)
BsrFI 536 ACCGGT [GA]^CCGG[CT]
BstEII 610 GGTGACC G^GT[ACGT]ACC
BstPI 610 GGTGACC G^GT[ACGT]ACC
BstXI 281 CCACACGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 536 ACCGGT [GA]^CCGG[CT]
DraII 92 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 724 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 92 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 610 GGTGACC G^GT[ACGT]ACC
EcoT14I 462 CCATGG C^C[AT][AT]GG
EcoT22I 59 ATGCAT ATGCA^T
FbaI 647 TGATCA T^GATCA
HinfI 184 GATTC G^A[ACGT]TC
KpnI 383 GGTACC GGTAC^C
KspI 263 CCGCGG CCGC^GG
MaeII 395 ACGT A^CGT
MluNI 344 TGGCCA TGG^CCA
MscI 344 TGGCCA TGG^CCA
NcoI 462 CCATGG C^CATGG
NsiI 59 ATGCAT ATGCA^T
NspI 640 GCATGT [GA]CATG^[CT]
PflFI 145 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 536 ACCGGT A^CCGGT
PmaCI 394 CACGTG CAC^GTG
PmlI 394 CACGTG CAC^GTG
PpuMI 92 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 743 CAGCTG CAG^CTG
RsaI 384 GTAC GT^AC
SacI 684 GAGCTC GAGCT^C
SacII 263 CCGCGG CCGC^GG
SapI 375 GCTCTTC GCTCTTC(1/4)
SfcI 471 CTACAG C^T[GA][CT]AG
SmaI 81 CCCGGG CCC^GGG
SmlI 88 CTCAAG C^T[CT][GA]AG
StyI 462 CCATGG C^C[AT][AT]GG
TaqI 41 TCGA T^CGA
TfiI 184 GATTC G^A[AT]TC
Tth111I 145 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 41 TCGA T^CGA
XmaCI 81 CCCGGG C^CCGGG
XmaI 81 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 72 ACGCGT A^C[GA][CT]GT
314 ACGCGT
AlwI 216 GATCC GGATC(4/5)
602 GGATC
AvaI 23 CCCGAG C^[CT]CG[GA]G
81 CCCGGG
BbeI 160 GGCGCC GGCGC^C
661 GGCGCC
BfaI 529 CTAG C^TAG
784 CTAG
BsmFI 115 GGGAC GGGAC(10/14)
94 GTCCC
BsoBI 23 CCCGAG C^[CT]CG[GA]G
81 CCCGGG
BsrI 433 ACTGG ACTGG(1/-1)
658 ACTGG
BstYI 215 AGATCC [GA]^GATC[CT]
602 GGATCT
DdeI 569 CTCAG C^T[ACGT]AG
682 CTGAG
EaeI 344 TGGCCA [CT]^GGCC[GA]
714 TGGCCG
EarI 376 CTCTTC CTCTTC(1/4)
705 GAAGAG
FauI 338 CCCGC CCCGC(4/6)
127 GCGGG
HaeII 160 GGCGCC [GA]GCGC^[CT]
661 GGCGCC
KasI 160 GGCGCC G^GCGCC
661 GGCGCC
Ksp632I 376 CTCTTC CTCTTC(1/4)
705 GAAGAG
MaeI 529 CTAG C^TAG
784 CTAG
MflI 215 AGATCC [GA]^GATC[CT]
602 GGATCT
MluI 72 ACGCGT A^CGCGT
314 ACGCGT
MspA1I 263 CCGCGG C[AC]G^C[GT]G
743 CAGCTG
NarI 160 GGCGCC GG^CGCC
661 GGCGCC
SfoI 160 GGCGCC GGC^GCC
661 GGCGCC
TspRI 680 CACTG CA[GC]TG[ACGT][ACGT]^
761 CAGTG
XhoII 215 AGATCC [GA]^GATC[CT]
602 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DsaI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I XcmI