Restriction map for pFN21AB6385
 (Linear) (Six-base) MAPSORT of: KIBB6385.seq  Check: 4442  from: 1  to: 819



KIBB6385.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AccI GT'mk_AC

Cuts at:      0     425     819 
   Size:        425     394
 
Acc65I G'GTAC_C

Cuts at:      0     383     819 
   Size:        383     436
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     363     819 
   Size:         25     338     456

  Fragments arranged by size: 

                 456     338      25
 
AflIII A'CryG_T

Cuts at:      0      72     314     819 
   Size:         72     242     505

  Fragments arranged by size: 

                 505     242      72
 
AgeI A'CCGG_T

Cuts at:      0     536     819 
   Size:        536     283
 
AleI CACnn'nnGTG

Cuts at:      0     398     819 
   Size:        398     421
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      62      94     819 
   Size:         62      32     725

  Fragments arranged by size: 

                 725      62      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     576     819 
   Size:        576     243
 
AvaI C'yCGr_G

Cuts at:      0      23      81     819 
   Size:         23      58     738

  Fragments arranged by size: 

                 738      58      23
 
BanI G'GyrC_C

Cuts at:      0     160     383     661     819 
   Size:        160     223     278     158

  Fragments arranged by size: 

                 278     223     160     158
 
BanII G_rGCy'C

Cuts at:      0      23      89     338     502     622     688     701     819 
   Size:         23      66     249     164     120      66      13     118

  Fragments arranged by size: 

                 249     164     120     118      66      66      23      13
            
 
BbeI G_GCGC'C

Cuts at:      0     164     665     819 
   Size:        164     501     154

  Fragments arranged by size: 

                 501     164     154
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     539     819 
   Size:        539     280
 
BclI T'GATC_A

Cuts at:      0     647     819 
   Size:        647     172
 
BfrBI ATG'CAT

Cuts at:      0      61     819 
   Size:         61     758
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     275     580     799     819 
   Size:         37     238     305     219      20

  Fragments arranged by size: 

                 305     238     219      37      20
 
Bpu10I CC'TnA_GC

Cuts at:      0     569     819 
   Size:        569     250
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     819 
   Size:         73     746
 
BsaAI yAC'GTr

Cuts at:      0     396     819 
   Size:        396     423
 
BsaHI Gr'CG_yC

Cuts at:      0     161     269     295     662     819 
   Size:        161     108      26     367     157

  Fragments arranged by size: 

                 367     161     157     108      26
 
BsaWI w'CCGG_w

Cuts at:      0     536     819 
   Size:        536     283
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      62      92     604     634     819 
   Size:         62      30     512      30     185

  Fragments arranged by size: 

                 512     185      62      30      30
 
BsiEI CG_ry'CG

Cuts at:      0     191     819 
   Size:        191     628
 
BsiHKAI G_wGCw'C

Cuts at:      0     688     819 
   Size:        688     131
 
Bsp1286I G_dGCh'C

Cuts at:      0      23      89     338     502     622     688     701     819 
   Size:         23      66     249     164     120      66      13     118

  Fragments arranged by size: 

                 249     164     120     118      66      66      23      13
            
 
BsrDI GCAATG_nn'

Cuts at:      0     636     819 
   Size:        636     183
 
BsrFI r'CCGG_y

Cuts at:      0     536     819 
   Size:        536     283
 
BstEII G'GTnAC_C

Cuts at:      0     610     819 
   Size:        610     209
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     288     819 
   Size:        288     531
 
BstYI r'GATC_y

Cuts at:      0     215     602     819 
   Size:        215     387     217

  Fragments arranged by size: 

                 387     217     215
 
BtgI C'CryG_G

Cuts at:      0     263     347     462     819 
   Size:        263      84     115     357

  Fragments arranged by size: 

                 357     263     115      84
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     108     210     389     819 
   Size:        108     102     179     430

  Fragments arranged by size: 

                 430     179     108     102
 
DraIII CAC_nnn'GTG

Cuts at:      0     729     819 
   Size:        729      90
 
EaeI y'GGCC_r

Cuts at:      0     344     714     819 
   Size:        344     370     105

  Fragments arranged by size: 

                 370     344     105
 
EarI CTCTTCn'nnn_

Cuts at:      0     382     700     819 
   Size:        382     318     119

  Fragments arranged by size: 

                 382     318     119
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     211     819 
   Size:        211     608
 
EcoICRI GAG'CTC

Cuts at:      0     686     819 
   Size:        686     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25      37     275     363     580     799     819 
   Size:         25      12     238      88     217     219      20

  Fragments arranged by size: 

                 238     219     217      88      25      20      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      93     819 
   Size:         93     726
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     237     819 
   Size:        237     582
 
HaeII r_GCGC'y

Cuts at:      0     164     665     819 
   Size:        164     501     154

  Fragments arranged by size: 

                 501     164     154
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     428     460     604     636     819 
   Size:        428      32     144      32     183

  Fragments arranged by size: 

                 428     183     144      32      32
 
KasI G'GCGC_C

Cuts at:      0     160     661     819 
   Size:        160     501     158

  Fragments arranged by size: 

                 501     160     158
 
KpnI G_GTAC'C

Cuts at:      0     387     819 
   Size:        387     432
 
MluI A'CGCG_T

Cuts at:      0      72     314     819 
   Size:         72     242     505

  Fragments arranged by size: 

                 505     242      72
 
MscI TGG'CCA

Cuts at:      0     346     819 
   Size:        346     473
 
MslI CAynn'nnrTG

Cuts at:      0     286     398     461     819 
   Size:        286     112      63     358

  Fragments arranged by size: 

                 358     286     112      63
 
MspA1I CmG'CkG

Cuts at:      0     265     745     819 
   Size:        265     480      74

  Fragments arranged by size: 

                 480     265      74
 
NarI GG'CG_CC

Cuts at:      0     161     662     819 
   Size:        161     501     157

  Fragments arranged by size: 

                 501     161     157
 
NcoI C'CATG_G

Cuts at:      0     462     819 
   Size:        462     357
 
NsiI A_TGCA'T

Cuts at:      0      63     819 
   Size:         63     756
 
NspI r_CATG'y

Cuts at:      0     644     819 
   Size:        644     175
 
PasI CC'CwG_GG

Cuts at:      0     495     819 
   Size:        495     324
 
PmlI CAC'GTG

Cuts at:      0     396     819 
   Size:        396     423
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      62      94     819 
   Size:         62      32     725

  Fragments arranged by size: 

                 725      62      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      93     819 
   Size:         93     726
 
PvuII CAG'CTG

Cuts at:      0     745     819 
   Size:        745      74
 
SacI G_AGCT'C

Cuts at:      0     688     819 
   Size:        688     131
 
SacII CC_GC'GG

Cuts at:      0     266     819 
   Size:        266     553
 
SapI GCTCTTCn'nnn_

Cuts at:      0     382     819 
   Size:        382     437
 
SfcI C'TryA_G

Cuts at:      0     471     819 
   Size:        471     348
 
SfoI GGC'GCC

Cuts at:      0     162     663     819 
   Size:        162     501     156

  Fragments arranged by size: 

                 501     162     156
 
SmaI CCC'GGG

Cuts at:      0      83     819 
   Size:         83     736
 
SmlI C'TyrA_G

Cuts at:      0      88     819 
   Size:         88     731
 
StyI C'CwwG_G

Cuts at:      0     462     819 
   Size:        462     357
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     177     388     598     705     819 
   Size:        177     211     210     107     114

  Fragments arranged by size: 

                 211     210     177     114     107
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     594     626     819 
   Size:        594      32     193

  Fragments arranged by size: 

                 594     193      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     148     819 
   Size:        148     671
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     129     288     730     819 
   Size:        129     159     442      89

  Fragments arranged by size: 

                 442     159     129      89
 
XmaI C'CCGG_G

Cuts at:      0      81     819 
   Size:         81     738
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AgeI     AleI     AloI    AlwNI
     AvaI     BanI    BanII     BbeI     BbsI     BclI    BfrBI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI
 Bsp1286I    BsrDI    BsrFI   BstEII    BstXI    BstYI     BtgI    BtgZI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I     KasI     KpnI     MluI     MscI     MslI   MspA1I
     NarI     NcoI     NsiI     NspI     PasI     PmlI     PpiI    PpuMI
    PvuII     SacI    SacII     SapI     SfcI     SfoI     SmaI     SmlI
     StyI    TaqII     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AhdI     AlfI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BsaI    BsaBI    BseRI    BseYI     BsgI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MmeI     NaeI
     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PciI    PflMI
     PfoI     PmeI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     XbaI     XhoI     XmnI     ZraI
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