Restriction map for
pFN21AE0481
(Linear) (Six-base) MAPSORT of: KIEE0481.seq Check: 1512 from: 1 to: 1257
KIEE0481.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AccI GT'mk_AC
Cuts at: 0 251 628 1257
Size: 251 377 629
Fragments arranged by size:
629 377 251
AlwNI CAG_nnn'CTG
Cuts at: 0 1201 1257
Size: 1201 56
ApoI r'AATT_y
Cuts at: 0 1007 1257
Size: 1007 250
AseI AT'TA_AT
Cuts at: 0 1103 1257
Size: 1103 154
AvaI C'yCGr_G
Cuts at: 0 338 1257
Size: 338 919
BamHI G'GATC_C
Cuts at: 0 352 878 1257
Size: 352 526 379
Fragments arranged by size:
526 379 352
BanII G_rGCy'C
Cuts at: 0 53 1172 1257
Size: 53 1119 85
Fragments arranged by size:
1119 85 53
BfrBI ATG'CAT
Cuts at: 0 313 1257
Size: 313 944
BmrI ACTGGGnnnn_n'
Cuts at: 0 658 682 887 961 1257
Size: 658 24 205 74 296
Fragments arranged by size:
658 296 205 74 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1063 1257
Size: 1063 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 451 503 1257
Size: 438 13 52 754
Fragments arranged by size:
754 438 52 13
Bpu10I CC'TnA_GC
Cuts at: 0 440 1257
Size: 440 817
BsaI GGTCTCn'nnnn_
Cuts at: 0 472 1257
Size: 472 785
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 410 694 1257
Size: 410 284 563
Fragments arranged by size:
563 410 284
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 1257
Size: 9 1248
Bsp1286I G_dGCh'C
Cuts at: 0 53 1172 1257
Size: 53 1119 85
Fragments arranged by size:
1119 85 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 95 1257
Size: 95 1162
BsrFI r'CCGG_y
Cuts at: 0 187 1080 1257
Size: 187 893 177
Fragments arranged by size:
893 187 177
BssSI C'ACGA_G
Cuts at: 0 900 1257
Size: 900 357
BstYI r'GATC_y
Cuts at: 0 352 878 1257
Size: 352 526 379
Fragments arranged by size:
526 379 352
BstZ17I GTA'TAC
Cuts at: 0 629 1257
Size: 629 628
BtgI C'CryG_G
Cuts at: 0 482 1257
Size: 482 775
BtsI GCAGTG_nn'
Cuts at: 0 95 687 966 1257
Size: 95 592 279 291
Fragments arranged by size:
592 291 279 95
DraI TTT'AAA
Cuts at: 0 74 1257
Size: 74 1183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1132 1257
Size: 1132 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 451 503 1257
Size: 438 13 52 754
Fragments arranged by size:
754 438 52 13
EcoO109I rG'GnC_Cy
Cuts at: 0 219 599 637 916 1257
Size: 219 380 38 279 341
Fragments arranged by size:
380 341 279 219 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 786 1257
Size: 786 471
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 534 566 1118 1150 1257
Size: 337 32 165 32 552 32 107
Fragments arranged by size:
552 337 165 107 32 32 32
NcoI C'CATG_G
Cuts at: 0 482 1257
Size: 482 775
NdeI CA'TA_TG
Cuts at: 0 243 1257
Size: 243 1014
NsiI A_TGCA'T
Cuts at: 0 315 1257
Size: 315 942
PasI CC'CwG_GG
Cuts at: 0 473 752 851 1257
Size: 473 279 99 406
Fragments arranged by size:
473 406 279 99
PpuMI rG'GwC_Cy
Cuts at: 0 219 599 637 916 1257
Size: 219 380 38 279 341
Fragments arranged by size:
380 341 279 219 38
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 793 825 1257
Size: 793 32 432
Fragments arranged by size:
793 432 32
PstI C_TGCA'G
Cuts at: 0 636 1257
Size: 636 621
SfcI C'TryA_G
Cuts at: 0 632 1257
Size: 632 625
SmaI CCC'GGG
Cuts at: 0 340 1257
Size: 340 917
StyI C'CwwG_G
Cuts at: 0 482 1257
Size: 482 775
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 604 956 1257
Size: 604 352 301
Fragments arranged by size:
604 352 301
Tth111I GACn'n_nGTC
Cuts at: 0 688 967 1257
Size: 688 279 290
Fragments arranged by size:
688 290 279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 1257
Size: 480 777
XmaI C'CCGG_G
Cuts at: 0 338 1257
Size: 338 919
XmnI GAAnn'nnTTC
Cuts at: 0 1112 1257
Size: 1112 145
Enzymes that do cut:
AccI AlwNI ApoI AseI AvaI BamHI BanII BfrBI
BmrI BplI BpmI Bpu10I BsaI BseRI BsgI Bsp1286I
BspMI BsrFI BssSI BstYI BstZ17I BtgI BtsI DraI
EciI Eco57MI EcoO109I FalI Hin4I NcoI NdeI NsiI
PasI PpuMI PsrI PstI SfcI SmaI StyI TaqII
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstXI Bsu36I BtgZI
ClaI CspCI DraIII DrdI EaeI EagI EarI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NheI NotI NruI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI XbaI XhoI ZraI