(Circular) (Six-base) MAPSORT of: pFN21AB5772.seq Check: 1653 from: 1 to: 6991 pFN21AB5772 6991 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4801 278 Size: 53 83 186 936 3265 2468 Fragments arranged by size: 3265 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2638 3498 3582 4334 1937 Size: 701 860 84 752 4594 Fragments arranged by size: 4594 860 752 701 84 Acc65I G'GTAC_C Cuts at: 4312 4312 Size: 6991 AclI AA'CG_TT Cuts at: 1563 5118 5491 1563 Size: 3555 373 3063 Fragments arranged by size: 3555 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 2885 2898 3141 3335 3874 3882 4118 Size: 638 615 13 243 194 539 8 236 Cuts at: 4118 5054 6438 6706 1632 Size: 936 1384 268 1917 Fragments arranged by size: 1917 1384 936 638 615 539 268 243 236 194 13 8 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6794 AflIII A'CryG_T Cuts at: 1203 5890 6867 1203 Size: 4687 977 1327 Fragments arranged by size: 4687 1327 977 AgeI A'CCGG_T Cuts at: 5822 5822 Size: 6991 AhdI GACnn_n'nnGTC Cuts at: 5720 5720 Size: 6991 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6991 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6991 AlwNI CAG_nnn'CTG Cuts at: 2509 4734 6306 2509 Size: 2225 1572 3194 Fragments arranged by size: 3194 2225 1572 ApaLI G'TGCA_C Cuts at: 5048 6204 5048 Size: 1156 5835 ApoI r'AATT_y Cuts at: 1493 2426 2869 3665 3915 4300 4526 4678 6787 Size: 933 443 796 250 385 226 152 2109 Cuts at: 6787 1493 Size: 1697 Fragments arranged by size: 2109 1697 933 796 443 385 250 226 152 AseI AT'TA_AT Cuts at: 160 1032 5545 160 Size: 872 4513 1606 Fragments arranged by size: 4513 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6991 AvaI C'yCGr_G Cuts at: 1870 1943 4316 1870 Size: 73 2373 4545 Fragments arranged by size: 4545 2373 73 BamHI G'GATC_C Cuts at: 2521 3513 4321 4688 2521 Size: 992 808 367 4824 Fragments arranged by size: 4824 992 808 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4312 5767 618 Size: 325 217 627 2525 1455 1842 Fragments arranged by size: 2525 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2220 3618 3933 4200 4310 1391 Size: 829 1398 315 267 110 4072 Fragments arranged by size: 4072 1398 829 315 267 110 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2333 928 Size: 971 434 5586 Fragments arranged by size: 5586 971 434 BbvCI CC'TCA_GC Cuts at: 2526 3762 2526 Size: 1236 5755 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5182 5216 1903 Size: 34 3245 34 3678 Fragments arranged by size: 3678 3245 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2866 4883 6093 2866 Size: 2017 1210 3764 Fragments arranged by size: 3764 2017 1210 BclI T'GATC_A Cuts at: 1548 3025 1548 Size: 1477 5514 BfrBI ATG'CAT Cuts at: 2553 2553 Size: 6991 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5602 136 Size: 107 122 71 631 605 3930 1525 Fragments arranged by size: 3930 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6986 6986 Size: 6991 BlpI GC'TnA_GC Cuts at: 2385 4408 2385 Size: 2023 4968 Bme1580I G_kGCm'C Cuts at: 1790 2773 5052 6208 1790 Size: 983 2279 1156 2573 Fragments arranged by size: 2573 2279 1156 983 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6991 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3389 3920 4722 Size: 416 53 83 399 24 1972 531 802 Cuts at: 4722 5680 442 Size: 958 1753 Fragments arranged by size: 1972 1753 958 802 531 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6991 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3547 3547 Size: 6991 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2204 2280 2446 3614 3640 3828 4242 Size: 884 81 76 166 1168 26 188 414 Cuts at: 4242 5651 1239 Size: 1409 2579 Fragments arranged by size: 2579 1409 1168 884 414 188 166 81 76 26 Bpu10I CC'TnA_GC Cuts at: 2526 3604 3762 2526 Size: 1078 158 5755 Fragments arranged by size: 5755 1078 158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 3383 4184 5116 5981 6279 2333 Size: 1050 801 932 865 298 3045 Fragments arranged by size: 3045 1050 932 865 801 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2347 5654 882 Size: 617 848 3307 2219 Fragments arranged by size: 3307 2219 848 617 BsaAI yAC'GTr Cuts at: 493 1204 4782 493 Size: 711 3578 2702 Fragments arranged by size: 3578 2702 711 BsaBI GATnn'nnATC Cuts at: 6985 6985 Size: 6991 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4798 5180 5815 275 Size: 53 83 186 936 3265 382 635 1451 Fragments arranged by size: 3265 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2322 5424 5822 6096 6243 6654 2322 Size: 3102 398 274 147 411 2659 Fragments arranged by size: 3102 2659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 2132 2162 2192 Size: 30 165 30 693 30 6 30 30 Cuts at: 2192 1178 Size: 5977 Fragments arranged by size: 5977 693 165 30 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2131 2228 2818 3536 1186 Size: 945 97 590 718 4641 Fragments arranged by size: 4641 945 718 590 97 BseYI C'CCAG_C Cuts at: 4087 4275 6194 6690 4087 Size: 188 1919 496 4388 Fragments arranged by size: 4388 1919 496 188 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6418 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4441 5202 6230 1540 Size: 275 186 2440 761 1028 2301 Fragments arranged by size: 2440 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 3014 3533 3618 3933 4200 5052 5137 6208 Size: 794 519 85 315 267 852 85 1071 Cuts at: 6208 2220 Size: 3003 Fragments arranged by size: 3003 1071 852 794 519 315 267 85 85 BsmI GAATG_Cn' Cuts at: 2555 4257 4502 4595 2555 Size: 1702 245 93 4951 Fragments arranged by size: 4951 1702 245 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2773 3014 3533 3618 3933 4200 Size: 399 430 553 241 519 85 315 267 Cuts at: 4200 4310 5052 5137 6208 1391 Size: 110 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 553 519 430 399 315 267 241 110 85 85 BspEI T'CCGG_A Cuts at: 6654 6654 Size: 6991 BspHI T'CATG_A Cuts at: 4880 6610 4880 Size: 1730 5261 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3711 4346 844 Size: 2867 635 3489 Fragments arranged by size: 3489 2867 635 BsrBI CCG'CTC Cuts at: 1394 1823 2143 4878 1394 Size: 429 320 2735 3507 Fragments arranged by size: 3507 2735 429 320 BsrDI GCAATG_nn' Cuts at: 65 1248 2196 5486 5660 65 Size: 1183 948 3290 174 1396 Fragments arranged by size: 3290 1396 1183 948 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5635 5822 1700 Size: 110 3825 187 2869 Fragments arranged by size: 3825 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2723 96 Size: 2627 4364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6991 BssSI C'ACGA_G Cuts at: 5051 6063 5051 Size: 1012 5979 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6991 BstBI TT'CG_AA Cuts at: 5795 5795 Size: 6991 BstEII G'GTnAC_C Cuts at: 1184 3767 1184 Size: 2583 4408 BstYI r'GATC_y Cuts at: 1972 2133 2521 3513 4321 4688 5073 5090 6542 Size: 161 388 992 808 367 385 17 1452 Cuts at: 6542 6628 6640 6986 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 992 808 388 385 367 346 161 86 17 12 BstZ17I GTA'TAC Cuts at: 3583 3583 Size: 6991 Bsu36I CC'TnA_GG Cuts at: 4041 4041 Size: 6991 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5500 Fragments arranged by size: 5500 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6252 Fragments arranged by size: 6252 683 56 BtsI GCAGTG_nn' Cuts at: 3047 4507 5301 5321 3047 Size: 1460 794 20 4717 Fragments arranged by size: 4717 1460 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6991 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6956 DraI TTT'AAA Cuts at: 4295 4295 Size: 6991 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5998 809 Size: 507 4682 1802 Fragments arranged by size: 4682 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2193 3327 4438 Size: 54 1425 50 275 18 363 1134 1111 Cuts at: 4438 5327 6956 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1134 1111 889 363 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4438 1537 Size: 275 2626 4090 Fragments arranged by size: 4090 2626 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3183 3394 3503 4921 1353 Size: 1830 211 109 1418 3423 Fragments arranged by size: 3423 1830 1418 211 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5578 5952 6098 387 Size: 1064 138 3989 374 146 1280 Fragments arranged by size: 3989 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2218 3616 3931 4198 2218 Size: 1398 315 267 5011 Fragments arranged by size: 5011 1398 315 267 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2204 2270 2280 2446 2885 2898 Size: 393 491 81 66 10 166 439 13 Cuts at: 2898 3141 3335 3614 3640 3828 3874 3882 4118 Size: 243 194 279 26 188 46 8 236 Cuts at: 4118 4242 5054 5651 6438 6706 1239 Size: 124 812 597 787 268 1524 Fragments arranged by size: 1524 812 787 597 491 439 393 279 268 243 236 194 188 166 124 81 66 46 26 13 10 8 EcoO109I rG'GnC_Cy Cuts at: 4205 4205 Size: 6991 EcoRI G'AATT_C Cuts at: 3915 4300 4678 3915 Size: 385 378 6228 Fragments arranged by size: 6228 385 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2327 2327 Size: 6991 FspI TGC'GCA Cuts at: 5497 5497 Size: 6991 HaeII r_GCGC'y Cuts at: 1678 6138 1678 Size: 4460 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2162 2194 2554 Size: 32 135 32 467 32 91 32 360 Cuts at: 2554 2586 2925 2957 3086 3118 3890 3922 4253 Size: 32 339 32 129 32 772 32 331 Cuts at: 4253 4285 5632 5664 5706 5738 1373 Size: 32 1347 32 42 32 2626 Fragments arranged by size: 2626 1347 772 467 360 339 331 135 129 91 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2639 3475 3784 3964 4335 4581 Size: 988 281 701 836 309 180 371 246 Cuts at: 4581 669 Size: 3079 Fragments arranged by size: 3079 988 836 701 371 309 281 246 180 HindIII A'AGCT_T Cuts at: 748 748 Size: 6991 HpaI GTT'AAC Cuts at: 4581 4581 Size: 6991 KpnI G_GTAC'C Cuts at: 4316 4316 Size: 6991 MfeI C'AATT_G Cuts at: 2719 4590 2719 Size: 1871 5120 MluI A'CGCG_T Cuts at: 6867 6867 Size: 6991 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4100 6105 6289 1305 Size: 2795 2005 184 2007 Fragments arranged by size: 2795 2007 2005 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2195 3329 10 Size: 54 1425 343 363 1134 3672 Fragments arranged by size: 3672 1425 1134 363 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2046 2681 4949 5308 5467 518 Size: 648 880 635 2268 359 159 2042 Fragments arranged by size: 2268 2042 880 648 635 359 159 MspA1I CmG'CkG Cuts at: 1606 2176 2239 3037 3313 4407 5084 6232 6477 Size: 570 63 798 276 1094 677 1148 245 Cuts at: 6477 1606 Size: 2120 Fragments arranged by size: 2120 1148 1094 798 677 570 276 245 63 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5500 Fragments arranged by size: 5500 939 552 NdeI CA'TA_TG Cuts at: 387 2265 387 Size: 1878 5113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6991 NotI GC'GGCC_GC Cuts at: 4438 4438 Size: 6991 NsiI A_TGCA'T Cuts at: 2555 2555 Size: 6991 NspI r_CATG'y Cuts at: 1132 1229 2557 3141 3254 3497 3589 4349 5894 Size: 97 1328 584 113 243 92 760 1545 Cuts at: 5894 1132 Size: 2229 Fragments arranged by size: 2229 1545 1328 760 584 243 113 97 92 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6991 PciI A'CATG_T Cuts at: 5890 5890 Size: 6991 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6991 PfoI T'CCnGG_A Cuts at: 2136 2136 Size: 6991 PmeI GTTT'AAAC Cuts at: 4295 4295 Size: 6991 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5060 5092 6573 6605 5060 Size: 32 1481 32 5446 Fragments arranged by size: 5446 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4205 4205 Size: 6991 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6991 PsiI TTA'TAA Cuts at: 4561 4561 Size: 6991 PstI C_TGCA'G Cuts at: 830 2253 3054 4343 830 Size: 1423 801 1289 3478 Fragments arranged by size: 3478 1423 1289 801 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6991 PvuII CAG'CTG Cuts at: 2176 2239 3037 3313 2176 Size: 63 798 276 5854 Fragments arranged by size: 5854 798 276 63 SacI G_AGCT'C Cuts at: 2220 3618 3933 4200 2220 Size: 1398 315 267 5011 Fragments arranged by size: 5011 1398 315 267 SalI G'TCGA_C Cuts at: 1936 2637 4333 1936 Size: 701 1696 4594 Fragments arranged by size: 4594 1696 701 SanDI GG'GwC_CC Cuts at: 4205 4205 Size: 6991 SapI GCTCTTCn'nnn_ Cuts at: 3183 3183 Size: 6991 SbfI CC_TGCA'GG Cuts at: 4343 4343 Size: 6991 ScaI AGT'ACT Cuts at: 5239 6951 5239 Size: 1712 5279 SfcI C'TryA_G Cuts at: 826 1045 2249 3050 3724 4339 5474 6155 6346 Size: 219 1204 801 674 615 1135 681 191 Cuts at: 6346 826 Size: 1471 Fragments arranged by size: 1471 1204 1135 801 681 674 615 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6991 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6991 SmaI CCC'GGG Cuts at: 1872 4318 1872 Size: 2446 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2312 3362 4199 5095 5996 6258 Size: 197 926 369 1050 837 896 901 262 Cuts at: 6258 820 Size: 1553 Fragments arranged by size: 1553 1050 926 901 896 837 369 262 197 SnaBI TAC'GTA Cuts at: 493 4782 493 Size: 4289 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6991 SphI G_CATG'C Cuts at: 1132 3141 4349 1132 Size: 2009 1208 3774 Fragments arranged by size: 3774 2009 1208 SspI AAT'ATT Cuts at: 5 52 4915 5 Size: 47 4863 2081 Fragments arranged by size: 4863 2081 47 StuI AGG'CCT Cuts at: 1517 2291 3599 1517 Size: 774 1308 4909 Fragments arranged by size: 4909 1308 774 StyI C'CwwG_G Cuts at: 513 1065 2004 2653 4430 513 Size: 552 939 649 1777 3074 Fragments arranged by size: 3074 1777 939 649 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2058 5018 5035 5188 5373 5818 325 Size: 1255 478 2960 17 153 185 445 1498 Fragments arranged by size: 2960 1498 1255 478 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2723 5237 6949 Size: 274 80 33 51 1443 746 2514 1712 Cuts at: 6949 96 Size: 138 Fragments arranged by size: 2514 1712 1443 746 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3820 3852 3906 3938 6951 6983 3820 Size: 32 54 32 3013 32 3828 Fragments arranged by size: 3828 3013 54 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6991 XbaI T'CTAG_A Cuts at: 3754 4327 3754 Size: 573 6418 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6991 XmaI C'CCGG_G Cuts at: 1870 4316 1870 Size: 2446 4545 XmnI GAAnn'nnTTC Cuts at: 2014 3306 3867 5120 2014 Size: 1292 561 1253 3885 Fragments arranged by size: 3885 1292 1253 561 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4799 276 Size: 53 83 186 936 3265 2468 Fragments arranged by size: 3265 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI