Restriction map for
pFN21AB5772
(Linear) (Six-base) MAPSORT of: KIBB5772.seq Check: 5234 from: 1 to: 2286
KIBB5772.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 0 633 1493 1577 2286
Size: 633 860 84 709
Fragments arranged by size:
860 709 633 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 880 893 1136 1330 1869 1877 2113
Size: 265 615 13 243 194 539 8 236
Cuts at: 2113 2286
Size: 173
Fragments arranged by size:
615 539 265 243 236 194 173 13
8
AlwNI CAG_nnn'CTG
Cuts at: 0 504 2286
Size: 504 1782
ApoI r'AATT_y
Cuts at: 0 421 864 1660 1910 2286
Size: 421 443 796 250 376
Fragments arranged by size:
796 443 421 376 250
BamHI G'GATC_C
Cuts at: 0 516 1508 2286
Size: 516 992 778
Fragments arranged by size:
992 778 516
BanII G_rGCy'C
Cuts at: 0 215 1613 1928 2195 2286
Size: 215 1398 315 267 91
Fragments arranged by size:
1398 315 267 215 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 328 2286
Size: 328 1958
BbvCI CC'TCA_GC
Cuts at: 0 521 1757 2286
Size: 521 1236 529
Fragments arranged by size:
1236 529 521
BciVI GTATCCnnnnn_n'
Cuts at: 0 861 2286
Size: 861 1425
BclI T'GATC_A
Cuts at: 0 1020 2286
Size: 1020 1266
BfrBI ATG'CAT
Cuts at: 0 548 2286
Size: 548 1738
BlpI GC'TnA_GC
Cuts at: 0 380 2286
Size: 380 1906
Bme1580I G_kGCm'C
Cuts at: 0 768 2286
Size: 768 1518
BmrI ACTGGGnnnn_n'
Cuts at: 0 1384 1915 2286
Size: 1384 531 371
Fragments arranged by size:
1384 531 371
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1542 2286
Size: 1542 744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 199 275 441 1609 1635 1823 2237
Size: 118 81 76 166 1168 26 188 414
Cuts at: 2237 2286
Size: 49
Fragments arranged by size:
1168 414 188 166 118 81 76 49
26
Bpu10I CC'TnA_GC
Cuts at: 0 521 1599 1757 2286
Size: 521 1078 158 529
Fragments arranged by size:
1078 529 521 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 1378 2179 2286
Size: 328 1050 801 107
Fragments arranged by size:
1050 801 328 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 342 2286
Size: 342 1944
BsaWI w'CCGG_w
Cuts at: 0 317 2286
Size: 317 1969
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 127 157 187 2286
Size: 91 30 6 30 30 2099
Fragments arranged by size:
2099 91 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 126 223 813 1531 2286
Size: 126 97 590 718 755
Fragments arranged by size:
755 718 590 126 97
BseYI C'CCAG_C
Cuts at: 0 2082 2270 2286
Size: 2082 188 16
Fragments arranged by size:
2082 188 16
BsiHKAI G_wGCw'C
Cuts at: 0 215 1009 1528 1613 1928 2195 2286
Size: 215 794 519 85 315 267 91
Fragments arranged by size:
794 519 315 267 215 91 85
BsmI GAATG_Cn'
Cuts at: 0 550 2252 2286
Size: 550 1702 34
Fragments arranged by size:
1702 550 34
Bsp1286I G_dGCh'C
Cuts at: 0 215 768 1009 1528 1613 1928 2195 2286
Size: 215 553 241 519 85 315 267 91
Fragments arranged by size:
553 519 315 267 241 215 91 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1706 2286
Size: 1706 580
BsrBI CCG'CTC
Cuts at: 0 138 2286
Size: 138 2148
BsrDI GCAATG_nn'
Cuts at: 0 191 2286
Size: 191 2095
BsrGI T'GTAC_A
Cuts at: 0 718 2286
Size: 718 1568
BstEII G'GTnAC_C
Cuts at: 0 1762 2286
Size: 1762 524
BstYI r'GATC_y
Cuts at: 0 128 516 1508 2286
Size: 128 388 992 778
Fragments arranged by size:
992 778 388 128
BstZ17I GTA'TAC
Cuts at: 0 1578 2286
Size: 1578 708
Bsu36I CC'TnA_GG
Cuts at: 0 2036 2286
Size: 2036 250
BtsI GCAGTG_nn'
Cuts at: 0 1042 2286
Size: 1042 1244
EaeI y'GGCC_r
Cuts at: 0 188 1322 2286
Size: 188 1134 964
Fragments arranged by size:
1134 964 188
EarI CTCTTCn'nnn_
Cuts at: 0 1178 1389 1498 2286
Size: 1178 211 109 788
Fragments arranged by size:
1178 788 211 109
EcoICRI GAG'CTC
Cuts at: 0 213 1611 1926 2193 2286
Size: 213 1398 315 267 93
Fragments arranged by size:
1398 315 267 213 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 199 265 275 441 880 893 1136
Size: 118 81 66 10 166 439 13 243
Cuts at: 1136 1330 1609 1635 1823 1869 1877 2113 2237
Size: 194 279 26 188 46 8 236 124
Cuts at: 2237 2286
Size: 49
Fragments arranged by size:
439 279 243 236 194 188 166 124
118 81 66 49 46 26 13 10
8
EcoO109I rG'GnC_Cy
Cuts at: 0 2200 2286
Size: 2200 86
EcoRI G'AATT_C
Cuts at: 0 1910 2286
Size: 1910 376
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 322 2286
Size: 322 1964
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 157 189 549 581 920 952
Size: 34 32 91 32 360 32 339 32
Cuts at: 952 1081 1113 1885 1917 2248 2280 2286
Size: 129 32 772 32 331 32 6
Fragments arranged by size:
772 360 339 331 129 91 34 32
32 32 32 32 32 32 6
HincII GTy'rAC
Cuts at: 0 634 1470 1779 1959 2286
Size: 634 836 309 180 327
Fragments arranged by size:
836 634 327 309 180
MfeI C'AATT_G
Cuts at: 0 714 2286
Size: 714 1572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2095 2286
Size: 2095 191
MscI TGG'CCA
Cuts at: 0 190 1324 2286
Size: 190 1134 962
Fragments arranged by size:
1134 962 190
MslI CAynn'nnrTG
Cuts at: 0 41 676 2286
Size: 41 635 1610
Fragments arranged by size:
1610 635 41
MspA1I CmG'CkG
Cuts at: 0 171 234 1032 1308 2286
Size: 171 63 798 276 978
Fragments arranged by size:
978 798 276 171 63
NdeI CA'TA_TG
Cuts at: 0 260 2286
Size: 260 2026
NsiI A_TGCA'T
Cuts at: 0 550 2286
Size: 550 1736
NspI r_CATG'y
Cuts at: 0 552 1136 1249 1492 1584 2286
Size: 552 584 113 243 92 702
Fragments arranged by size:
702 584 552 243 113 92
PfoI T'CCnGG_A
Cuts at: 0 131 2286
Size: 131 2155
PpuMI rG'GwC_Cy
Cuts at: 0 2200 2286
Size: 2200 86
PstI C_TGCA'G
Cuts at: 0 248 1049 2286
Size: 248 801 1237
Fragments arranged by size:
1237 801 248
PvuII CAG'CTG
Cuts at: 0 171 234 1032 1308 2286
Size: 171 63 798 276 978
Fragments arranged by size:
978 798 276 171 63
SacI G_AGCT'C
Cuts at: 0 215 1613 1928 2195 2286
Size: 215 1398 315 267 91
Fragments arranged by size:
1398 315 267 215 91
SalI G'TCGA_C
Cuts at: 0 632 2286
Size: 632 1654
SanDI GG'GwC_CC
Cuts at: 0 2200 2286
Size: 2200 86
SapI GCTCTTCn'nnn_
Cuts at: 0 1178 2286
Size: 1178 1108
SfcI C'TryA_G
Cuts at: 0 244 1045 1719 2286
Size: 244 801 674 567
Fragments arranged by size:
801 674 567 244
SmlI C'TyrA_G
Cuts at: 0 307 1357 2194 2286
Size: 307 1050 837 92
Fragments arranged by size:
1050 837 307 92
SphI G_CATG'C
Cuts at: 0 1136 2286
Size: 1136 1150
StuI AGG'CCT
Cuts at: 0 286 1594 2286
Size: 286 1308 692
Fragments arranged by size:
1308 692 286
StyI C'CwwG_G
Cuts at: 0 648 2286
Size: 648 1638
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 53 2286
Size: 53 2233
TatI w'GTAC_w
Cuts at: 0 718 2286
Size: 718 1568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1815 1847 1901 1933 2286
Size: 1815 32 54 32 353
Fragments arranged by size:
1815 353 54 32 32
XbaI T'CTAG_A
Cuts at: 0 1749 2286
Size: 1749 537
XmnI GAAnn'nnTTC
Cuts at: 0 9 1301 1862 2286
Size: 9 1292 561 424
Fragments arranged by size:
1292 561 424 9
Enzymes that do cut:
AccI AcuI AlwNI ApoI BamHI BanII BbsI BbvCI
BciVI BclI BfrBI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI BstEII BstYI
BstZ17I Bsu36I BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI FalI Hin4I HincII MfeI MmeI MscI MslI
MspA1I NdeI NsiI NspI PfoI PpuMI PstI PvuII
SacI SalI SanDI SapI SfcI SmlI SphI StuI
StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BcgI BglI
BglII BmgBI BmtI BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrFI BssHII BssSI BstAPI
BstBI BstXI BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XcmI XhoI XmaI ZraI