(Circular) (Six-base) MAPSORT of: pFN21AB6135.seq Check: 4936 from: 1 to: 6481 pFN21AB6135 6481 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2589 3824 1937 Size: 652 1235 4594 Fragments arranged by size: 4594 1235 652 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 3147 4608 4981 1563 Size: 1584 1461 373 3063 Fragments arranged by size: 3063 1584 1461 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3155 4544 5928 6196 1632 Size: 1523 1389 1384 268 1917 Fragments arranged by size: 1917 1523 1389 1384 268 AfeI AGC'GCT Cuts at: 2414 2516 2414 Size: 102 6379 AflII C'TTAA_G Cuts at: 820 1017 2696 820 Size: 197 1679 4605 Fragments arranged by size: 4605 1679 197 AflIII A'CryG_T Cuts at: 1203 2142 2910 3549 5380 6357 1203 Size: 939 768 639 1831 977 1327 Fragments arranged by size: 1831 1327 977 939 768 639 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 2973 3541 5210 2973 Size: 568 1669 4244 Fragments arranged by size: 4244 1669 568 AleI CACnn'nnGTG Cuts at: 1166 2093 1166 Size: 927 5554 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6481 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3005 3037 3275 3307 3005 Size: 32 238 32 6179 Fragments arranged by size: 6179 238 32 32 AlwNI CAG_nnn'CTG Cuts at: 4224 5796 4224 Size: 1572 4909 ApaI G_GGCC'C Cuts at: 3215 3215 Size: 6481 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 2832 3790 4016 4168 6277 1493 Size: 1339 958 226 152 2109 1697 Fragments arranged by size: 2109 1697 1339 958 226 152 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 2293 3806 1870 Size: 73 350 1513 4545 Fragments arranged by size: 4545 1513 350 73 BamHI G'GATC_C Cuts at: 3432 3811 4178 3432 Size: 379 367 5735 Fragments arranged by size: 5735 379 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3802 5257 618 Size: 325 217 627 2015 1455 1842 Fragments arranged by size: 2015 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2124 3215 3672 3800 1391 Size: 733 1091 457 128 4072 Fragments arranged by size: 4072 1091 733 457 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2340 928 Size: 971 441 5069 Fragments arranged by size: 5069 971 441 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3680 3714 4672 4706 1903 Size: 34 50 34 1659 34 958 34 3678 Fragments arranged by size: 3678 1659 958 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4373 5583 4373 Size: 1210 5271 BclI T'GATC_A Cuts at: 1548 1548 Size: 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5092 136 Size: 107 122 71 631 605 3420 1525 Fragments arranged by size: 3420 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3081 6476 3081 Size: 3395 3086 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 3171 3215 4542 5698 1790 Size: 1381 44 1327 1156 2573 Fragments arranged by size: 2573 1381 1327 1156 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2899 3580 4212 Size: 416 53 83 399 24 1482 681 632 Cuts at: 4212 5170 442 Size: 958 1753 Fragments arranged by size: 1753 1482 958 681 632 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2568 3437 3602 5141 1239 Size: 1329 869 165 1539 2579 Fragments arranged by size: 2579 1539 1329 869 165 Bpu10I CC'TnA_GC Cuts at: 2304 2304 Size: 6481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2042 2162 4606 5471 5769 2042 Size: 120 2444 865 298 2754 Fragments arranged by size: 2754 2444 865 298 120 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5144 882 Size: 617 3645 2219 Fragments arranged by size: 3645 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2911 4272 493 Size: 711 1707 1361 2702 Fragments arranged by size: 2702 1707 1361 711 BsaBI GATnn'nnATC Cuts at: 3086 6475 3086 Size: 3389 3092 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 2607 4288 4670 Size: 53 83 186 936 567 507 1681 382 Cuts at: 4670 5305 275 Size: 635 1451 Fragments arranged by size: 1681 1451 936 635 567 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3429 4914 5312 5586 5733 6144 3429 Size: 1485 398 274 147 411 3766 Fragments arranged by size: 3766 1485 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3275 3305 1178 Size: 30 165 30 1872 30 4354 Fragments arranged by size: 4354 1872 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 2639 3068 3296 3785 1186 Size: 946 507 429 228 489 3882 Fragments arranged by size: 3882 946 507 489 429 228 BseYI C'CCAG_C Cuts at: 2009 3313 5684 6180 2009 Size: 1304 2371 496 2310 Fragments arranged by size: 2371 2310 1304 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2138 2281 2464 3089 3695 1163 Size: 573 402 143 183 625 606 3949 Fragments arranged by size: 3949 625 606 573 402 183 143 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2047 3078 3931 4692 5720 1540 Size: 275 186 46 1031 853 761 1028 2301 Fragments arranged by size: 2301 1031 1028 853 761 275 186 46 BsiHKAI G_wGCw'C Cuts at: 2124 2300 3060 3233 4542 4627 5698 2124 Size: 176 760 173 1309 85 1071 2907 Fragments arranged by size: 2907 1309 1071 760 176 173 85 BsmI GAATG_Cn' Cuts at: 3992 4085 3992 Size: 93 6388 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2300 3060 3171 3215 3233 3672 Size: 399 334 176 760 111 44 18 439 Cuts at: 3672 3800 4542 4627 5698 1391 Size: 128 742 85 1071 2174 Fragments arranged by size: 2174 1071 760 742 439 399 334 176 128 111 85 44 18 BspEI T'CCGG_A Cuts at: 3429 6144 3429 Size: 2715 3766 BspHI T'CATG_A Cuts at: 4370 6100 4370 Size: 1730 4751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2549 3836 844 Size: 1705 1287 3489 Fragments arranged by size: 3489 1705 1287 BsrBI CCG'CTC Cuts at: 1394 1823 2040 2170 4368 1394 Size: 429 217 130 2198 3507 Fragments arranged by size: 3507 2198 429 217 130 BsrDI GCAATG_nn' Cuts at: 65 1248 4976 5150 65 Size: 1183 3728 174 1396 Fragments arranged by size: 3728 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 3513 5125 5312 1700 Size: 110 208 1495 1612 187 2869 Fragments arranged by size: 2869 1612 1495 208 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6481 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 3107 4541 5553 3107 Size: 1434 1012 4035 Fragments arranged by size: 4035 1434 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2493 3237 794 Size: 1699 744 4038 Fragments arranged by size: 4038 1699 744 BstBI TT'CG_AA Cuts at: 3729 5285 3729 Size: 1556 4925 BstEII G'GTnAC_C Cuts at: 1184 2198 1184 Size: 1014 5467 BstXI CCAn_nnnn'nTGG Cuts at: 2848 2848 Size: 6481 BstYI r'GATC_y Cuts at: 1972 3081 3432 3811 4178 4563 4580 6032 6118 Size: 1109 351 379 367 385 17 1452 86 Cuts at: 6118 6130 6476 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1109 385 379 367 351 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3051 3051 Size: 6481 BtgI C'CryG_G Cuts at: 513 1065 2521 513 Size: 552 1456 4473 Fragments arranged by size: 4473 1456 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3073 3241 487 Size: 683 56 1847 168 3727 Fragments arranged by size: 3727 1847 683 168 56 BtsI GCAGTG_nn' Cuts at: 3997 4791 4811 3997 Size: 794 20 5667 Fragments arranged by size: 5667 794 20 ClaI AT'CG_AT Cuts at: 1554 2709 1554 Size: 1155 5326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6446 DraI TTT'AAA Cuts at: 3471 3483 3785 3471 Size: 12 302 6167 Fragments arranged by size: 6167 302 12 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 2392 3075 Size: 54 1425 50 275 18 214 348 683 Cuts at: 3075 3339 3515 3928 4817 6446 8 Size: 264 176 413 889 1629 43 Fragments arranged by size: 1629 1425 889 683 413 348 275 264 214 176 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2044 3075 3928 1537 Size: 275 232 1031 853 4090 Fragments arranged by size: 4090 1031 853 275 232 EarI CTCTTCn'nnn_ Cuts at: 1353 2229 2405 4411 1353 Size: 876 176 2006 3423 Fragments arranged by size: 3423 2006 876 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 2234 5068 5442 5588 387 Size: 1064 138 453 192 2834 374 146 1280 Fragments arranged by size: 2834 1280 1064 453 374 192 146 138 EcoICRI GAG'CTC Cuts at: 2122 2122 Size: 6481 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2568 3155 3437 3602 4544 5141 5928 Size: 393 936 587 282 165 942 597 787 Cuts at: 5928 6196 1239 Size: 268 1524 Fragments arranged by size: 1524 942 936 787 597 587 393 282 268 165 EcoO109I rG'GnC_Cy Cuts at: 2461 2692 3013 3130 3212 3490 2461 Size: 231 321 117 82 278 5452 Fragments arranged by size: 5452 321 278 231 117 82 EcoRI G'AATT_C Cuts at: 3790 4168 3790 Size: 378 6103 FspI TGC'GCA Cuts at: 4987 4987 Size: 6481 HaeII r_GCGC'y Cuts at: 1678 2416 2518 3314 3349 3599 5628 1678 Size: 738 102 796 35 250 2029 2531 Fragments arranged by size: 2531 2029 796 738 250 102 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2909 2941 2959 2991 3527 Size: 32 135 32 1337 32 18 32 536 Cuts at: 3527 3559 5122 5154 5196 5228 1373 Size: 32 1563 32 42 32 2626 Fragments arranged by size: 2626 1563 1337 536 135 42 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3004 3825 4071 669 Size: 988 281 1066 821 246 3079 Fragments arranged by size: 3079 1066 988 821 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6481 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 4080 4080 Size: 6481 MluI A'CGCG_T Cuts at: 3549 6357 3549 Size: 2808 3673 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2926 5595 5779 1305 Size: 1621 2669 184 2007 Fragments arranged by size: 2669 2007 1621 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4659 Fragments arranged by size: 4659 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2093 2846 4439 4798 4957 518 Size: 648 927 753 1593 359 159 2042 Fragments arranged by size: 2042 1593 927 753 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2536 3679 3897 4574 5722 5967 1606 Size: 930 1143 218 677 1148 245 2120 Fragments arranged by size: 2120 1148 1143 930 677 245 218 NaeI GCC'GGC Cuts at: 3515 3515 Size: 6481 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5929 NdeI CA'TA_TG Cuts at: 387 2553 387 Size: 2166 4315 NgoMIV G'CCGG_C Cuts at: 3513 3513 Size: 6481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 2044 3928 2044 Size: 1884 4597 NruI TCG'CGA Cuts at: 2468 2468 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 2146 3031 3196 3839 5384 1132 Size: 97 917 885 165 643 1545 2229 Fragments arranged by size: 2229 1545 917 885 643 165 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 2142 5380 2142 Size: 3238 3243 PflMI CCAn_nnn'nTGG Cuts at: 1108 2367 3524 1108 Size: 1259 1157 4065 Fragments arranged by size: 4065 1259 1157 PfoI T'CCnGG_A Cuts at: 2326 2657 3153 3414 2326 Size: 331 496 261 5393 Fragments arranged by size: 5393 496 331 261 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PmlI CAC'GTG Cuts at: 2911 2911 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3275 3307 4550 4582 6063 6095 3275 Size: 32 1243 32 1481 32 3661 Fragments arranged by size: 3661 1481 1243 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2692 3013 3130 3490 2692 Size: 321 117 360 5683 Fragments arranged by size: 5683 360 321 117 PshAI GACnn'nnGTC Cuts at: 1613 2145 3633 1613 Size: 532 1488 4461 Fragments arranged by size: 4461 1488 532 PsiI TTA'TAA Cuts at: 4051 4051 Size: 6481 PspOMI G'GGCC_C Cuts at: 3211 3211 Size: 6481 PstI C_TGCA'G Cuts at: 830 2546 2754 3833 830 Size: 1716 208 1079 3478 Fragments arranged by size: 3478 1716 1079 208 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 3679 3679 Size: 6481 SacI G_AGCT'C Cuts at: 2124 2124 Size: 6481 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SbfI CC_TGCA'GG Cuts at: 2546 3833 2546 Size: 1287 5194 ScaI AGT'ACT Cuts at: 4729 6441 4729 Size: 1712 4769 SfcI C'TryA_G Cuts at: 826 1045 2479 2542 2750 3124 3829 4964 5645 Size: 219 1434 63 208 374 705 1135 681 Cuts at: 5645 5836 826 Size: 191 1471 Fragments arranged by size: 1471 1434 1135 705 681 374 219 208 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6481 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2057 2177 2696 4585 5486 5748 Size: 197 926 114 120 519 1889 901 262 Cuts at: 5748 820 Size: 1553 Fragments arranged by size: 1889 1553 926 901 519 262 197 120 114 SnaBI TAC'GTA Cuts at: 493 4272 493 Size: 3779 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6481 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 4405 5 Size: 47 4353 2081 Fragments arranged by size: 4353 2081 47 StuI AGG'CCT Cuts at: 1517 2274 3207 1517 Size: 757 933 4791 Fragments arranged by size: 4791 933 757 StyI C'CwwG_G Cuts at: 513 1065 2796 3920 513 Size: 552 1731 1124 3074 Fragments arranged by size: 3074 1731 1124 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 4508 4525 4678 4863 5308 325 Size: 1255 593 2335 17 153 185 445 1498 Fragments arranged by size: 2335 1498 1255 593 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4727 6439 96 Size: 274 80 33 51 1443 2750 1712 138 Fragments arranged by size: 2750 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6441 6473 6441 Size: 32 6449 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6481 XbaI T'CTAG_A Cuts at: 3817 3817 Size: 6481 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2803 2803 Size: 6481 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6481 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 4610 4610 Size: 6481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI