Restriction map for
pFN21AB6497
(Circular) (Six-base) MAPSORT of: pFN21AB6497.seq Check: 1939 from: 1 to: 5758
pFN21AB6497 5758 bp
With 182 enzymes: SgfI *
October 20, 2009 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2539 2818 2539
Size: 279 5479
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 3821 5205 5473 1632
Size: 896 1293 1384 268 1917
Fragments arranged by size:
1917 1384 1293 896 268
AfeI AGC'GCT
Cuts at: 2846 2846
Size: 5758
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 2969 3501 5073 2969
Size: 532 1572 3654
Fragments arranged by size:
3654 1572 532
ApaI G_GGCC'C
Cuts at: 2677 2677
Size: 5758
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 3067 3293 3445 5554 1493
Size: 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2513 2627 3083 1870
Size: 73 570 114 456 4545
Fragments arranged by size:
4545 570 456 114 73
AvrII C'CTAG_G
Cuts at: 2677 2677
Size: 5758
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2190 3079 4534 618
Size: 325 217 627 403 889 1455 1842
Fragments arranged by size:
1842 1455 889 627 403 325 217
BanII G_rGCy'C
Cuts at: 1391 2165 2430 2677 3012 3077 1391
Size: 774 265 247 335 65 4072
Fragments arranged by size:
4072 774 335 265 247 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2414 2448 3949 3983 1903
Size: 34 477 34 1501 34 3678
Fragments arranged by size:
3678 1501 477 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2742 5753 2742
Size: 3011 2747
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2677 3819 4975 1790
Size: 887 1142 1156 2573
Fragments arranged by size:
2573 1156 1142 887
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2102 2410 2102
Size: 308 5450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 4418 1239
Size: 1070 2109 2579
Fragments arranged by size:
2579 2109 1070
Bpu10I CC'TnA_GC
Cuts at: 2144 2639 2144
Size: 495 5263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2786 3883 4748 5046 2786
Size: 1097 865 298 3498
Fragments arranged by size:
3498 1097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2192 4421 882
Size: 617 693 2229 2219
Fragments arranged by size:
2229 2219 693 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 3549 493
Size: 711 1170 1175 2702
Fragments arranged by size:
2702 1175 1170 711
BsaBI GATnn'nnATC
Cuts at: 2918 3020 5752 2918
Size: 102 2732 2924
Fragments arranged by size:
2924 2732 102
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3012 4191 4589 4863 5010 5421 3012
Size: 1179 398 274 147 411 3349
Fragments arranged by size:
3349 1179 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2091 2513 3014 1186
Size: 871 34 422 501 3930
Fragments arranged by size:
3930 871 501 422 34
BseYI C'CCAG_C
Cuts at: 2152 4961 5457 2152
Size: 2809 496 2453
Fragments arranged by size:
2809 2453 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2301 3208 3969 4997 1540
Size: 275 186 300 907 761 1028 2301
Fragments arranged by size:
2301 1028 907 761 300 275 186
BsiHKAI G_wGCw'C
Cuts at: 2268 2688 3012 3819 3904 4975 2268
Size: 420 324 807 85 1071 3051
Fragments arranged by size:
3051 1071 807 420 324 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
BsmBI CGTCTCn'nnnn_
Cuts at: 2764 2764
Size: 5758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2165 2268 2430 2677 2688 3012 3077
Size: 399 375 103 162 247 11 324 65
Cuts at: 3077 3819 3904 4975 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 324 247 162
103 85 65 11
BspEI T'CCGG_A
Cuts at: 3012 5421 3012
Size: 2409 3349
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2248 2539 2553 2818 3113 844
Size: 1404 291 14 265 295 3489
Fragments arranged by size:
3489 1404 295 291 265 14
BsrBI CCG'CTC
Cuts at: 1394 1823 2108 3645 1394
Size: 429 285 1537 3507
Fragments arranged by size:
3507 1537 429 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2201 4402 4589 1700
Size: 110 391 2201 187 2869
Fragments arranged by size:
2869 2201 391 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2812 794
Size: 2018 3740
BstBI TT'CG_AA
Cuts at: 2136 4562 2136
Size: 2426 3332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2223 2742 3015 3088 3455 3840 3857 5309
Size: 251 519 273 73 367 385 17 1452
Cuts at: 5309 5395 5407 5753 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 519 385 367 346 273 251
86 73 17 12
Bsu36I CC'TnA_GG
Cuts at: 2858 2858
Size: 5758
BtgI C'CryG_G
Cuts at: 513 1065 2004 2661 2703 513
Size: 552 939 657 42 3568
Fragments arranged by size:
3568 939 657 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2463 2700
Size: 54 1425 50 275 18 376 257 237
Cuts at: 2700 3205 4094 5723 8
Size: 505 889 1629 43
Fragments arranged by size:
1629 1425 889 505 376 275 257 237
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3000 3688 1353
Size: 1647 688 3423
Fragments arranged by size:
3423 1647 688
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3010 3010
Size: 5758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2309 2528 3821 4418 5205 5473 1239
Size: 393 677 219 1293 597 787 268 1524
Fragments arranged by size:
1524 1293 787 677 597 393 268 219
EcoNI CCTnn'n_nnAGG
Cuts at: 2825 2825
Size: 5758
EcoO109I rG'GnC_Cy
Cuts at: 2607 2674 2966 2607
Size: 67 292 5399
Fragments arranged by size:
5399 292 67
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 2848 4905 1678
Size: 1170 2057 2531
Fragments arranged by size:
2531 2057 1170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373
Size: 32 135 32 2827 32 42 32 2626
Fragments arranged by size:
2827 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2392 4872 5056 1305
Size: 1087 2480 184 2007
Fragments arranged by size:
2480 2007 1087 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3716 4075 4234 518
Size: 648 2550 359 159 2042
Fragments arranged by size:
2550 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2910 3174 3851 4999 5244 1606
Size: 1304 264 677 1148 245 2120
Fragments arranged by size:
2120 1304 1148 677 264 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2969 1348
Size: 1621 4137
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2607 2607
Size: 5758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PspOMI G'GGCC_C
Cuts at: 2673 2673
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2295 2550 2829 3110 830
Size: 1465 255 279 281 3478
Fragments arranged by size:
3478 1465 281 279 255
PvuI CG_AT'CG
Cuts at: 2001 2301 2001
Size: 300 5458
PvuII CAG'CTG
Cuts at: 2910 2910
Size: 5758
SacI G_AGCT'C
Cuts at: 3012 3012
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SanDI GG'GwC_CC
Cuts at: 2607 2607
Size: 5758
SapI GCTCTTCn'nnn_
Cuts at: 3000 3000
Size: 5758
SbfI CC_TGCA'GG
Cuts at: 2295 2550 3110 2295
Size: 255 560 4943
Fragments arranged by size:
4943 560 255
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SexAI A'CCwGG_T
Cuts at: 2358 2358
Size: 5758
SfcI C'TryA_G
Cuts at: 826 1045 2089 2291 2402 2546 2825 3106 4241
Size: 219 1044 202 111 144 279 281 1135
Cuts at: 4241 4922 5113 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1044 681 281 279 219 202
191 144 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2801 3862 4763 5025 820
Size: 197 926 858 1061 901 262 1553
Fragments arranged by size:
1553 1061 926 901 858 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2004 2677 3039 3197 513
Size: 552 939 673 362 158 3074
Fragments arranged by size:
3074 939 673 552 362 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3785 3802 3955 4140 4585 325
Size: 1255 2205 17 153 185 445 1498
Fragments arranged by size:
2205 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2958 4004 5716
Size: 274 80 33 51 1443 981 1046 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1443 1046 981 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 2057 3887 2057
Size: 1830 3928
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BstXI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI