Restriction map for
pFN21AB5408
(Linear) (Six-base) MAPSORT of: KIBB5408.seq Check: 9487 from: 1 to: 1251
KIBB5408.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 0 62 1251
Size: 62 1189
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1251
Size: 40 1211
AflII C'TTAA_G
Cuts at: 0 649 1251
Size: 649 602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 1251
Size: 94 1157
AlwNI CAG_nnn'CTG
Cuts at: 0 276 961 1251
Size: 276 685 290
Fragments arranged by size:
685 290 276
ApaLI G'TGCA_C
Cuts at: 0 133 1251
Size: 133 1118
ApoI r'AATT_y
Cuts at: 0 426 803 1251
Size: 426 377 448
Fragments arranged by size:
448 426 377
AseI AT'TA_AT
Cuts at: 0 158 1251
Size: 158 1093
AvrII C'CTAG_G
Cuts at: 0 942 1251
Size: 942 309
BanI G'GyrC_C
Cuts at: 0 8 15 103 546 934 1251
Size: 8 7 88 443 388 317
Fragments arranged by size:
443 388 317 88 8 7
BanII G_rGCy'C
Cuts at: 0 36 1082 1251
Size: 36 1046 169
Fragments arranged by size:
1046 169 36
BbeI G_GCGC'C
Cuts at: 0 12 19 107 1251
Size: 12 7 88 1144
Fragments arranged by size:
1144 88 12 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 1251
Size: 605 646
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 359 1251
Size: 325 34 892
Fragments arranged by size:
892 325 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1155 1251
Size: 1155 96
Bme1580I G_kGCm'C
Cuts at: 0 137 551 955 1251
Size: 137 414 404 296
Fragments arranged by size:
414 404 296 137
BmrI ACTGGGnnnn_n'
Cuts at: 0 83 1251
Size: 83 1168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 577 589 979 1210 1251
Size: 533 44 12 390 231 41
Fragments arranged by size:
533 390 231 44 41 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 1251
Size: 189 1062
BsaAI yAC'GTr
Cuts at: 0 1011 1251
Size: 1011 240
BsaHI Gr'CG_yC
Cuts at: 0 9 16 104 1251
Size: 9 7 88 1147
Fragments arranged by size:
1147 88 9 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 439 469 1251
Size: 439 30 782
Fragments arranged by size:
782 439 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 113 1251
Size: 113 1138
BseYI C'CCAG_C
Cuts at: 0 67 366 395 550 1251
Size: 67 299 29 155 701
Fragments arranged by size:
701 299 155 67 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 460 676 1251
Size: 169 291 216 575
Fragments arranged by size:
575 291 216 169
BsiHKAI G_wGCw'C
Cuts at: 0 137 1082 1251
Size: 137 945 169
Fragments arranged by size:
945 169 137
BsmBI CGTCTCn'nnnn_
Cuts at: 0 912 1251
Size: 912 339
Bsp1286I G_dGCh'C
Cuts at: 0 36 137 551 955 1082 1251
Size: 36 101 414 404 127 169
Fragments arranged by size:
414 404 169 127 101 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 740 750 1251
Size: 740 10 501
Fragments arranged by size:
740 501 10
BsrBI CCG'CTC
Cuts at: 0 388 1251
Size: 388 863
BsrFI r'CCGG_y
Cuts at: 0 5 322 1251
Size: 5 317 929
Fragments arranged by size:
929 317 5
BssSI C'ACGA_G
Cuts at: 0 400 1251
Size: 400 851
BstAPI GCAn_nnn'nTGC
Cuts at: 0 141 438 1146 1251
Size: 141 297 708 105
Fragments arranged by size:
708 297 141 105
BstEII G'GTnAC_C
Cuts at: 0 630 1251
Size: 630 621
BstXI CCAn_nnnn'nTGG
Cuts at: 0 222 778 1251
Size: 222 556 473
Fragments arranged by size:
556 473 222
BtgI C'CryG_G
Cuts at: 0 635 1023 1251
Size: 635 388 228
Fragments arranged by size:
635 388 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 69 1251
Size: 69 1182
BtsI GCAGTG_nn'
Cuts at: 0 463 1251
Size: 463 788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 873 908 1251
Size: 873 35 343
Fragments arranged by size:
873 343 35
EaeI y'GGCC_r
Cuts at: 0 894 1251
Size: 894 357
EarI CTCTTCn'nnn_
Cuts at: 0 796 1251
Size: 796 455
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 110 1173 1251
Size: 110 1063 78
Fragments arranged by size:
1063 110 78
EcoICRI GAG'CTC
Cuts at: 0 1080 1251
Size: 1080 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 533 577 589 979 1210 1251
Size: 40 493 44 12 390 231 41
Fragments arranged by size:
493 390 231 44 41 40 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 559 1251
Size: 559 692
EcoO109I rG'GnC_Cy
Cuts at: 0 340 613 645 1088 1251
Size: 340 273 32 443 163
Fragments arranged by size:
443 340 273 163 32
HaeII r_GCGC'y
Cuts at: 0 12 19 107 168 819 1251
Size: 12 7 88 61 651 432
Fragments arranged by size:
651 432 88 61 12 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 1251
Size: 439 32 780
Fragments arranged by size:
780 439 32
HincII GTy'rAC
Cuts at: 0 63 1251
Size: 63 1188
KasI G'GCGC_C
Cuts at: 0 8 15 103 1251
Size: 8 7 88 1148
Fragments arranged by size:
1148 88 8 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1251
Size: 887 364
MscI TGG'CCA
Cuts at: 0 896 1251
Size: 896 355
MslI CAynn'nnrTG
Cuts at: 0 191 1033 1251
Size: 191 842 218
Fragments arranged by size:
842 218 191
MspA1I CmG'CkG
Cuts at: 0 678 690 1137 1251
Size: 678 12 447 114
Fragments arranged by size:
678 447 114 12
NaeI GCC'GGC
Cuts at: 0 7 1251
Size: 7 1244
NarI GG'CG_CC
Cuts at: 0 9 16 104 1251
Size: 9 7 88 1147
Fragments arranged by size:
1147 88 9 7
NcoI C'CATG_G
Cuts at: 0 1023 1251
Size: 1023 228
NgoMIV G'CCGG_C
Cuts at: 0 5 1251
Size: 5 1246
PpuMI rG'GwC_Cy
Cuts at: 0 645 1088 1251
Size: 645 443 163
Fragments arranged by size:
645 443 163
PstI C_TGCA'G
Cuts at: 0 146 252 470 751 1251
Size: 146 106 218 281 500
Fragments arranged by size:
500 281 218 146 106
PvuII CAG'CTG
Cuts at: 0 678 690 1137 1251
Size: 678 12 447 114
Fragments arranged by size:
678 447 114 12
SacI G_AGCT'C
Cuts at: 0 1082 1251
Size: 1082 169
SalI G'TCGA_C
Cuts at: 0 61 1251
Size: 61 1190
SanDI GG'GwC_CC
Cuts at: 0 1088 1251
Size: 1088 163
SbfI CC_TGCA'GG
Cuts at: 0 751 1251
Size: 751 500
SfcI C'TryA_G
Cuts at: 0 142 248 466 747 1251
Size: 142 106 218 281 504
Fragments arranged by size:
504 281 218 142 106
SfoI GGC'GCC
Cuts at: 0 10 17 105 1251
Size: 10 7 88 1146
Fragments arranged by size:
1146 88 10 7
SmlI C'TyrA_G
Cuts at: 0 168 649 1251
Size: 168 481 602
Fragments arranged by size:
602 481 168
StuI AGG'CCT
Cuts at: 0 655 745 947 1251
Size: 655 90 202 304
Fragments arranged by size:
655 304 202 90
StyI C'CwwG_G
Cuts at: 0 329 942 1023 1083 1251
Size: 329 613 81 60 168
Fragments arranged by size:
613 329 168 81 60
Tth111I GACn'n_nGTC
Cuts at: 0 885 1251
Size: 885 366
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 1251
Size: 219 1032
Enzymes that do cut:
AccI AcuI AflII AlfI AlwNI ApaLI ApoI AseI
AvrII BanI BanII BbeI BbsI BcgI BglI Bme1580I
BmrI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI
BstAPI BstEII BstXI BtgI BtgZI BtsI CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PpuMI PstI PvuII SacI SalI SanDI
SbfI SfcI SfoI SmlI StuI StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI ApaI AscI AsiSI AvaI BaeI BamHI
BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaBI BsaWI BsiEI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BssHII BstBI BstYI BstZ17I
Bsu36I ClaI DraI DraIII DrdI EagI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SapI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI TstI XbaI
XhoI XmaI XmnI ZraI