Restriction map for
pFN21AE0042
##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflIII AhdI Alw44I AlwI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BglI BlnI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SexAI SfiI SfoI
SfuI SnaBI SphI SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 280 CGCG CG^CG
AcyI 1120 GACGCC G[GA]^CG[CT]C
AflII 1393 CTTAAG C^TTAAG
AgeI 399 ACCGGT A^CCGGT
AocI 900 CCTGAGG CC^T[ACGT]AGG
Asp700I 817 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 671 CCCGGG C^[CT]CG[GA]G
BanI 402 GGTGCC G^G[CT][GA]CC
BcgI 1272 CGACAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1393 CTTAAG C^TTAAG
BglII 545 AGATCT A^GATCT
BsaAI 658 TACGTG [CT]AC^GT[GA]
BsaHI 1120 GACGCC G[GA]^CG[CT]C
BsmI 358 GAATGC GAATGC(1/-1)
BsoBI 671 CCCGGG C^[CT]CG[GA]G
Bsp1407I 975 TGTACA T^GTACA
BspHI 1308 TCATGA T^CATGA
BsrFI 399 ACCGGT [GA]^CCGG[CT]
BsrGI 975 TGTACA T^GTACA
BstAPI 687 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 280 CGCG CG^CG
BstXI 201 CCAGGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 545 AGATCT [GA]^GATC[CT]
Bsu36I 900 CCTGAGG CC^T[ACGT]AGG
Cfr10I 399 ACCGGT [GA]^CCGG[CT]
DrdI 1192 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 900 CCTGAGG CC^T[ACGT]AGG
EcoNI 218 CCTGCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 765 GCGGG CCCGC(4/6)
HgaI 1120 GACGC GACGC(5/10)
Hin1I 1120 GACGCC G[GA]^CG[CT]C
HindIII 300 AAGCTT A^AGCTT
MflI 545 AGATCT [GA]^GATC[CT]
MseI 1394 TTAA T^TAA
MspA1I 566 CAGCTG C[AC]G^C[GT]G
MvnI 280 CGCG CG^CG
PinAI 399 ACCGGT A^CCGGT
PshAI 174 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1466 CTGCAG CTGCA^G
PvuII 566 CAGCTG CAG^CTG
RcaI 1308 TCATGA T^CATGA
SbfI 1465 CCTGCAGG CCTGCA^GG
ScaI 344 AGTACT AGT^ACT
SgrAI 398 CACCGGTG C[GA]^CCGG[CT]G
SmaI 671 CCCGGG CCC^GGG
SmlI 1393 CTTAAG C^T[CT][GA]AG
SpeI 469 ACTAGT A^CTAGT
Sse8387I 1465 CCTGCAGG CCTGCA^GG
SspBI 975 TGTACA T^GTACA
TfiI 1042 GAATC G^A[AT]TC
Tru9I 1394 TTAA T^TAA
XcmI 137 CCAATGCAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 545 AGATCT [GA]^GATC[CT]
XmaCI 671 CCCGGG C^CCGGG
XmaI 671 CCCGGG C^CCGGG
XmnI 817 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BalI 210 TGGCCA TGG^CCA
836 TGGCCA
BbsI 171 GAAGAC GAAGAC(2/6)
895 GAAGAC
BbvI 142 GCAGC GCAGC(8/12)
762 GCTGC
BfaI 224 CTAG C^TAG
470 CTAG
BpuAI 171 GAAGAC GAAGAC(2/6)
895 GAAGAC
BsaWI 231 ACCGGA [AT]^CCGG[AT]
399 ACCGGT
DpnII 36 GATC ^GATC
546 GATC
DraII 906 GGGTCCC [GA]G^G[ACGT]CC[CT]
1199 AGGTCCC
EcoO109I 906 GGGTCCC [GA]G^G[ACGT]CC[CT]
1199 AGGTCCC
HaeII 429 GGCGCT [GA]GCGC^[CT]
794 AGCGCC
MaeI 224 CTAG C^TAG
470 CTAG
MaeII 485 ACGT A^CGT
659 ACGT
MaeIII 160 GTGAC ^GT[ACGT]AC
482 GTGAC
MboI 36 GATC ^GATC
546 GATC
MfeI 20 CAATTG C^AATTG
1231 CAATTG
MluNI 210 TGGCCA TGG^CCA
836 TGGCCA
MscI 210 TGGCCA TGG^CCA
836 TGGCCA
MslI 396 CACACCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
450 CATGGAGGTG
MunI 20 CAATTG C^AATTG
1231 CAATTG
NdeII 36 GATC ^GATC
546 GATC
NspI 806 GCATGT [GA]CATG^[CT]
1388 ACATGC
PleI 235 GACTC GAGTC(4/5)
331 GAGTC
PpuMI 906 GGGTCCC [GA]G^G[AT]CC[CT]
1199 AGGTCCC
SacI 1369 GAGCTC GAGCT^C
1444 GAGCTC
Sau3AI 36 GATC ^GATC
546 GATC
SfcI 1125 CTACAG C^T[GA][CT]AG
1466 CTGCAG
StuI 1031 AGGCCT AGG^CCT
1286 AGGCCT
TseI 142 GCAGC G^C[AT]GC
762 GCTGC
Tsp45I 160 GTGAC ^GT[GC]AC
482 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BanII BcnI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MboII MnlI MspI MvaI MwoI
NciI NcoI NlaIII NlaIV PflMI
RsaI Sau96I ScrFI SfaNI StyI
Tsp509I TspRI Van91I