(Circular) (Six-base) MAPSORT of: pFN21AE0042.seq Check: 612 from: 1 to: 6238 pFN21AE0042 6238 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3581 1937 Size: 1644 4594 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 4301 5685 5953 1632 Size: 662 2007 1384 268 1917 Fragments arranged by size: 2007 1917 1384 662 268 AflII C'TTAA_G Cuts at: 820 1017 3398 820 Size: 197 2381 3660 Fragments arranged by size: 3660 2381 197 AflIII A'CryG_T Cuts at: 1203 5137 6114 1203 Size: 3934 977 1327 Fragments arranged by size: 3934 1327 977 AgeI A'CCGG_T Cuts at: 2404 5069 2404 Size: 2665 3573 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 2405 1166 Size: 1239 4999 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2312 2344 2312 Size: 32 6206 AlwNI CAG_nnn'CTG Cuts at: 2698 2887 3445 3981 5553 2698 Size: 189 558 536 1572 3383 Fragments arranged by size: 3383 1572 558 536 189 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 3547 3773 3925 6034 1493 Size: 2054 226 152 2109 1697 Fragments arranged by size: 2109 2054 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4792 160 Size: 872 3760 1606 Fragments arranged by size: 3760 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 2676 3563 1870 Size: 73 733 887 4545 Fragments arranged by size: 4545 887 733 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 3559 5014 618 Size: 325 217 627 620 1152 1455 1842 Fragments arranged by size: 1842 1455 1152 627 620 325 217 BanII G_rGCy'C Cuts at: 1391 2265 3378 3423 3453 3557 1391 Size: 874 1113 45 30 104 4072 Fragments arranged by size: 4072 1113 874 104 45 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2183 2907 928 Size: 971 284 724 4259 Fragments arranged by size: 4259 971 724 284 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3266 3300 4429 4463 1903 Size: 34 1329 34 1129 34 3678 Fragments arranged by size: 3678 1329 1129 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 1548 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2550 6233 2550 Size: 3683 2555 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 2301 2412 4299 5455 1790 Size: 511 111 1887 1156 2573 Fragments arranged by size: 2573 1887 1156 511 111 BmgBI CAC'GTC Cuts at: 1318 2491 1318 Size: 1173 5065 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3969 4927 442 Size: 416 53 83 399 24 2552 958 1753 Fragments arranged by size: 2552 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3053 3362 3467 4898 1239 Size: 1814 309 105 1431 2579 Fragments arranged by size: 2579 1814 1431 309 105 Bpu10I CC'TnA_GC Cuts at: 3080 3417 3080 Size: 337 5901 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4363 5228 5526 4363 Size: 865 298 5075 Fragments arranged by size: 5075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2196 2227 2633 3223 4901 882 Size: 617 697 31 406 590 1678 2219 Fragments arranged by size: 2219 1678 697 617 590 406 31 BsaAI yAC'GTr Cuts at: 493 1204 2665 4029 493 Size: 711 1461 1364 2702 Fragments arranged by size: 2702 1461 1364 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3126 4045 4427 5062 Size: 53 83 186 936 1593 919 382 635 Cuts at: 5062 275 Size: 1451 Fragments arranged by size: 1593 1451 936 919 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2236 2404 4671 5069 5343 5490 5901 2236 Size: 168 2267 398 274 147 411 2573 Fragments arranged by size: 2573 2267 411 398 274 168 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3435 3465 1178 Size: 30 165 30 2032 30 3951 Fragments arranged by size: 3951 2032 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2233 2696 2862 3269 1186 Size: 1047 463 166 407 4155 Fragments arranged by size: 4155 1047 463 407 166 BseYI C'CCAG_C Cuts at: 5441 5937 5441 Size: 496 5742 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2761 2789 3185 1163 Size: 573 1025 28 396 4216 Fragments arranged by size: 4216 1025 573 396 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3086 3378 3453 4299 4384 5455 3086 Size: 292 75 846 85 1071 3869 Fragments arranged by size: 3869 1071 846 292 85 75 BsmI GAATG_Cn' Cuts at: 2369 3749 3842 2369 Size: 1380 93 4765 Fragments arranged by size: 4765 1380 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2265 2301 2412 3086 3378 3423 3453 Size: 399 475 36 111 674 292 45 30 Cuts at: 3453 3557 4299 4384 5455 1391 Size: 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 674 475 399 292 111 104 85 45 36 30 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 3313 4127 5857 3313 Size: 814 1730 3694 Fragments arranged by size: 3694 1730 814 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2706 2872 3464 3593 844 Size: 1862 166 592 129 3489 Fragments arranged by size: 3489 1862 592 166 129 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4733 4907 65 Size: 1183 3485 174 1396 Fragments arranged by size: 3485 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2404 4882 5069 1700 Size: 110 594 2478 187 2869 Fragments arranged by size: 2869 2478 594 187 110 BsrGI T'GTAC_A Cuts at: 96 2980 96 Size: 2884 3354 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 2698 794 Size: 1904 4334 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstXI CCAn_nnnn'nTGG Cuts at: 2213 2213 Size: 6238 BstYI r'GATC_y Cuts at: 1972 2550 3568 3935 4320 4337 5789 5875 5887 Size: 578 1018 367 385 17 1452 86 12 Cuts at: 5887 6233 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1018 578 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2906 2906 Size: 6238 BtgI C'CryG_G Cuts at: 513 1065 2151 2250 2374 2412 3157 513 Size: 552 1086 99 124 38 745 3594 Fragments arranged by size: 3594 1086 745 552 124 99 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2509 487 Size: 683 56 1283 4216 Fragments arranged by size: 4216 1283 683 56 BtsI GCAGTG_nn' Cuts at: 3526 3754 4548 4568 3526 Size: 228 794 20 5196 Fragments arranged by size: 5196 794 228 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 3542 3542 Size: 6238 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3203 5245 809 Size: 507 1887 2042 1802 Fragments arranged by size: 2042 1887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 2247 2841 Size: 54 1425 50 275 18 385 32 594 Cuts at: 2841 3685 4574 6203 8 Size: 844 889 1629 43 Fragments arranged by size: 1629 1425 889 844 594 385 275 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2849 3246 4168 1353 Size: 731 765 397 922 3423 Fragments arranged by size: 3423 922 765 731 397 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3376 3451 3376 Size: 75 6163 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 3053 3362 3467 4301 4898 5685 Size: 393 662 759 309 105 834 597 787 Cuts at: 5685 5953 1239 Size: 268 1524 Fragments arranged by size: 1524 834 787 759 662 597 393 309 268 105 EcoNI CCTnn'n_nnAGG Cuts at: 2227 2227 Size: 6238 EcoO109I rG'GnC_Cy Cuts at: 2912 3205 2912 Size: 293 5945 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2146 2980 2146 Size: 834 5404 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 2438 2803 5385 1678 Size: 760 365 2582 2531 Fragments arranged by size: 2582 2531 760 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3248 3280 4879 4911 4953 Size: 32 135 32 1676 32 1599 32 42 Cuts at: 4953 4985 1373 Size: 32 2626 Fragments arranged by size: 2626 1676 1599 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3582 3828 669 Size: 988 281 1644 246 3079 Fragments arranged by size: 3079 1644 988 281 246 HindIII A'AGCT_T Cuts at: 748 2305 748 Size: 1557 4681 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 2025 3236 3837 2025 Size: 1211 601 4426 Fragments arranged by size: 4426 1211 601 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3483 5352 5536 1305 Size: 2178 1869 184 2007 Fragments arranged by size: 2178 2007 1869 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2217 2843 10 Size: 54 1425 343 385 626 3405 Fragments arranged by size: 3405 1425 626 385 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2405 2459 4196 4555 4714 518 Size: 648 1239 54 1737 359 159 2042 Fragments arranged by size: 2042 1737 1239 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2573 3654 4331 5479 5724 1606 Size: 967 1081 677 1148 245 2120 Fragments arranged by size: 2120 1148 1081 967 677 245 NcoI C'CATG_G Cuts at: 513 1065 2250 2374 3157 513 Size: 552 1185 124 783 3594 Fragments arranged by size: 3594 1185 783 552 124 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 2815 3397 3596 5141 1132 Size: 97 1586 582 199 1545 2229 Fragments arranged by size: 2229 1586 1545 582 199 97 PasI CC'CwG_GG Cuts at: 1348 3184 3211 1348 Size: 1836 27 4375 Fragments arranged by size: 4375 1836 27 PciI A'CATG_T Cuts at: 5137 5137 Size: 6238 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 2375 3073 1108 Size: 1209 58 698 4273 Fragments arranged by size: 4273 1209 698 58 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2912 3205 2912 Size: 293 5945 PshAI GACnn'nnGTC Cuts at: 1613 2183 1613 Size: 570 5668 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PstI C_TGCA'G Cuts at: 830 3475 3590 830 Size: 2645 115 3478 Fragments arranged by size: 3478 2645 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 PvuII CAG'CTG Cuts at: 2573 2573 Size: 6238 SacI G_AGCT'C Cuts at: 3378 3453 3378 Size: 75 6163 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SanDI GG'GwC_CC Cuts at: 2912 2912 Size: 6238 SbfI CC_TGCA'GG Cuts at: 3475 3590 3475 Size: 115 6123 ScaI AGT'ACT Cuts at: 2351 4486 6198 2351 Size: 2135 1712 2391 Fragments arranged by size: 2391 2135 1712 SfcI C'TryA_G Cuts at: 826 1045 3130 3471 3586 4721 5402 5593 826 Size: 219 2085 341 115 1135 681 191 1471 Fragments arranged by size: 2085 1471 1135 681 341 219 191 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 2404 1700 Size: 704 5534 SmaI CCC'GGG Cuts at: 1872 2678 3565 1872 Size: 806 887 4545 Fragments arranged by size: 4545 887 806 SmlI C'TyrA_G Cuts at: 820 1017 1943 3398 4342 5243 5505 820 Size: 197 926 1455 944 901 262 1553 Fragments arranged by size: 1553 1455 944 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 2474 152 Size: 2322 3916 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 3038 3293 1517 Size: 1521 255 4462 Fragments arranged by size: 4462 1521 255 StyI C'CwwG_G Cuts at: 513 1065 2250 2374 2517 3157 3222 3677 513 Size: 552 1185 124 143 640 65 455 3074 Fragments arranged by size: 3074 1185 640 552 455 143 124 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2126 2573 4265 4282 4435 4620 5065 Size: 1255 546 447 1692 17 153 185 445 Cuts at: 5065 325 Size: 1498 Fragments arranged by size: 1692 1498 1255 546 447 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2349 2980 4484 Size: 274 80 33 51 1443 372 631 1504 Cuts at: 4484 6196 96 Size: 1712 138 Fragments arranged by size: 1712 1504 1443 631 372 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2064 2096 2798 2830 6198 6230 2064 Size: 32 702 32 3368 32 2072 Fragments arranged by size: 3368 2072 702 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6238 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2149 2011 Size: 138 6100 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 2676 3563 1870 Size: 806 887 4545 Fragments arranged by size: 4545 887 806 XmnI GAAnn'nnTTC Cuts at: 2826 4367 2826 Size: 1541 4697 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI