Restriction map for
pFN21AE0042
(Linear) (Six-base) MAPSORT of: KIEE0042.seq Check: 2041 from: 1 to: 1533
KIEE0042.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1533
Size: 289 1244
AflII C'TTAA_G
Cuts at: 0 1393 1533
Size: 1393 140
AgeI A'CCGG_T
Cuts at: 0 399 1533
Size: 399 1134
AleI CACnn'nnGTG
Cuts at: 0 400 1533
Size: 400 1133
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 1533
Size: 307 32 1194
Fragments arranged by size:
1194 307 32
AlwNI CAG_nnn'CTG
Cuts at: 0 693 882 1440 1533
Size: 693 189 558 93
Fragments arranged by size:
693 558 189 93
AvaI C'yCGr_G
Cuts at: 0 671 1533
Size: 671 862
BanI G'GyrC_C
Cuts at: 0 402 1533
Size: 402 1131
BanII G_rGCy'C
Cuts at: 0 260 1373 1418 1448 1533
Size: 260 1113 45 30 85
Fragments arranged by size:
1113 260 85 45 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 178 902 1533
Size: 178 724 631
Fragments arranged by size:
724 631 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1261 1295 1533
Size: 1261 34 238
Fragments arranged by size:
1261 238 34
BglII A'GATC_T
Cuts at: 0 545 1533
Size: 545 988
Bme1580I G_kGCm'C
Cuts at: 0 296 407 1533
Size: 296 111 1126
Fragments arranged by size:
1126 296 111
BmgBI CAC'GTC
Cuts at: 0 486 1533
Size: 486 1047
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 1533
Size: 201 1332
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1357 1462 1533
Size: 1048 309 105 71
Fragments arranged by size:
1048 309 105 71
Bpu10I CC'TnA_GC
Cuts at: 0 1075 1412 1533
Size: 1075 337 121
Fragments arranged by size:
1075 337 121
BsaI GGTCTCn'nnnn_
Cuts at: 0 191 222 628 1218 1533
Size: 191 31 406 590 315
Fragments arranged by size:
590 406 315 191 31
BsaAI yAC'GTr
Cuts at: 0 660 1533
Size: 660 873
BsaHI Gr'CG_yC
Cuts at: 0 1121 1533
Size: 1121 412
BsaWI w'CCGG_w
Cuts at: 0 231 399 1533
Size: 231 168 1134
Fragments arranged by size:
1134 231 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1430 1460 1533
Size: 1430 30 73
Fragments arranged by size:
1430 73 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 228 691 857 1264 1533
Size: 228 463 166 407 269
Fragments arranged by size:
463 407 269 228 166
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 784 1180 1533
Size: 756 28 396 353
Fragments arranged by size:
756 396 353 28
BsiHKAI G_wGCw'C
Cuts at: 0 1081 1373 1448 1533
Size: 1081 292 75 85
Fragments arranged by size:
1081 292 85 75
BsmI GAATG_Cn'
Cuts at: 0 364 1533
Size: 364 1169
Bsp1286I G_dGCh'C
Cuts at: 0 260 296 407 1081 1373 1418 1448 1533
Size: 260 36 111 674 292 45 30 85
Fragments arranged by size:
674 292 260 111 85 45 36 30
BspHI T'CATG_A
Cuts at: 0 1308 1533
Size: 1308 225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 701 867 1459 1533
Size: 701 166 592 74
Fragments arranged by size:
701 592 166 74
BsrFI r'CCGG_y
Cuts at: 0 399 1533
Size: 399 1134
BsrGI T'GTAC_A
Cuts at: 0 975 1533
Size: 975 558
BstAPI GCAn_nnn'nTGC
Cuts at: 0 693 1533
Size: 693 840
BstXI CCAn_nnnn'nTGG
Cuts at: 0 208 1533
Size: 208 1325
BstYI r'GATC_y
Cuts at: 0 545 1533
Size: 545 988
Bsu36I CC'TnA_GG
Cuts at: 0 901 1533
Size: 901 632
BtgI C'CryG_G
Cuts at: 0 146 245 369 407 1152 1533
Size: 146 99 124 38 745 381
Fragments arranged by size:
745 381 146 124 99 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 504 1533
Size: 504 1029
BtsI GCAGTG_nn'
Cuts at: 0 1521 1533
Size: 1521 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 1198 1533
Size: 1198 335
EaeI y'GGCC_r
Cuts at: 0 210 242 836 1533
Size: 210 32 594 697
Fragments arranged by size:
697 594 210 32
EarI CTCTTCn'nnn_
Cuts at: 0 79 844 1241 1533
Size: 79 765 397 292
Fragments arranged by size:
765 397 292 79
EcoICRI GAG'CTC
Cuts at: 0 1371 1446 1533
Size: 1371 75 87
Fragments arranged by size:
1371 87 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1048 1357 1462 1533
Size: 289 759 309 105 71
Fragments arranged by size:
759 309 289 105 71
EcoNI CCTnn'n_nnAGG
Cuts at: 0 222 1533
Size: 222 1311
EcoO109I rG'GnC_Cy
Cuts at: 0 907 1200 1533
Size: 907 293 333
Fragments arranged by size:
907 333 293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 141 975 1533
Size: 141 834 558
Fragments arranged by size:
834 558 141
HaeII r_GCGC'y
Cuts at: 0 433 798 1533
Size: 433 365 735
Fragments arranged by size:
735 433 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1243 1275 1533
Size: 1243 32 258
Fragments arranged by size:
1243 258 32
HindIII A'AGCT_T
Cuts at: 0 300 1533
Size: 300 1233
MfeI C'AATT_G
Cuts at: 0 20 1231 1533
Size: 20 1211 302
Fragments arranged by size:
1211 302 20
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1478 1533
Size: 1478 55
MscI TGG'CCA
Cuts at: 0 212 838 1533
Size: 212 626 695
Fragments arranged by size:
695 626 212
MslI CAynn'nnrTG
Cuts at: 0 400 454 1533
Size: 400 54 1079
Fragments arranged by size:
1079 400 54
MspA1I CmG'CkG
Cuts at: 0 568 1533
Size: 568 965
NcoI C'CATG_G
Cuts at: 0 245 369 1152 1533
Size: 245 124 783 381
Fragments arranged by size:
783 381 245 124
NspI r_CATG'y
Cuts at: 0 810 1392 1533
Size: 810 582 141
Fragments arranged by size:
810 582 141
PasI CC'CwG_GG
Cuts at: 0 1179 1206 1533
Size: 1179 27 327
Fragments arranged by size:
1179 327 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 370 1068 1533
Size: 312 58 698 465
Fragments arranged by size:
698 465 312 58
PpuMI rG'GwC_Cy
Cuts at: 0 907 1200 1533
Size: 907 293 333
Fragments arranged by size:
907 333 293
PshAI GACnn'nnGTC
Cuts at: 0 178 1533
Size: 178 1355
PstI C_TGCA'G
Cuts at: 0 1470 1533
Size: 1470 63
PvuII CAG'CTG
Cuts at: 0 568 1533
Size: 568 965
SacI G_AGCT'C
Cuts at: 0 1373 1448 1533
Size: 1373 75 85
Fragments arranged by size:
1373 85 75
SanDI GG'GwC_CC
Cuts at: 0 907 1533
Size: 907 626
SbfI CC_TGCA'GG
Cuts at: 0 1470 1533
Size: 1470 63
ScaI AGT'ACT
Cuts at: 0 346 1533
Size: 346 1187
SfcI C'TryA_G
Cuts at: 0 1125 1466 1533
Size: 1125 341 67
Fragments arranged by size:
1125 341 67
SgrAI Cr'CCGG_yG
Cuts at: 0 399 1533
Size: 399 1134
SmaI CCC'GGG
Cuts at: 0 673 1533
Size: 673 860
SmlI C'TyrA_G
Cuts at: 0 1393 1533
Size: 1393 140
SpeI A'CTAG_T
Cuts at: 0 469 1533
Size: 469 1064
StuI AGG'CCT
Cuts at: 0 1033 1288 1533
Size: 1033 255 245
Fragments arranged by size:
1033 255 245
StyI C'CwwG_G
Cuts at: 0 245 369 512 1152 1217 1533
Size: 245 124 143 640 65 316
Fragments arranged by size:
640 316 245 143 124 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 121 568 1533
Size: 121 447 965
Fragments arranged by size:
965 447 121
TatI w'GTAC_w
Cuts at: 0 344 975 1533
Size: 344 631 558
Fragments arranged by size:
631 558 344
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 59 91 793 825 1533
Size: 59 32 702 32 708
Fragments arranged by size:
708 702 59 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 1533
Size: 144 1389
XmaI C'CCGG_G
Cuts at: 0 671 1533
Size: 671 862
XmnI GAAnn'nnTTC
Cuts at: 0 821 1533
Size: 821 712
Enzymes that do cut:
AcuI AflII AgeI AleI AloI AlwNI AvaI BanI
BanII BbsI BcgI BglII Bme1580I BmgBI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HindIII MfeI MmeI MscI MslI MspA1I NcoI NspI
PasI PflMI PpuMI PshAI PstI PvuII SacI SanDI
SbfI ScaI SfcI SgrAI SmaI SmlI SpeI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AhdI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BlpI BmrI BmtI BpuEI BsaBI BseYI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BssHII BssSI BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI
SexAI SfiI SfoI SgfI SnaBI SphI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI