Restriction map for
pFN21AB6426
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BanII BbrPI
BbvCI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MseI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 351 CGCG CG^CG
AhdI 403 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 460 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 403 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 563 TGGCCA TGG^CCA
BbsI 622 GAAGAC GAAGAC(2/6)
BcgI 633 CGAGTTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 399 CCCGG CC^[GC]GG
BglI 383 GCCACGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 371 AGATCT A^GATCT
BpmI 809 CTGGAG CTGGAG(16/14)
BpuAI 622 GAAGAC GAAGAC(2/6)
BsgI 505 GTGCAG GTGCAG(16/14)
BsiEI 525 CGACCG CG[GA][CT]^CG
BsrI 69 CCAGT ACTGG(1/-1)
BssSI 241 CACGAG CACGAG(-5/-1)
BstUI 351 CGCG CG^CG
DdeI 669 CTCAG C^T[ACGT]AG
DraII 461 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 610 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 526 GACCGTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 403 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 461 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 167 CCTTGG C^C[AT][AT]GG
HapII 400 CCGG C^CGG
HpaII 400 CCGG C^CGG
MaeII 503 ACGT A^CGT
MluNI 563 TGGCCA TGG^CCA
MscI 563 TGGCCA TGG^CCA
MspA1I 652 CAGCTG C[AC]G^C[GT]G
MspI 400 CCGG C^CGG
MvnI 351 CGCG CG^CG
NciI 399 CCCGG CC^[GC]GG
PleI 28 GACTC GAGTC(4/5)
PstI 406 CTGCAG CTGCA^G
PvuII 652 CAGCTG CAG^CTG
ScaI 71 AGTACT AGT^ACT
SexAI 611 ACCAGGT A^CC[AT]GGT
SfcI 406 CTGCAG C^T[GA][CT]AG
SmlI 754 CTCAAG C^T[CT][GA]AG
StuI 390 AGGCCT AGG^CCT
StyI 167 CCTTGG C^C[AT][AT]GG
TaqI 31 TCGA T^CGA
TfiI 423 GATTC G^A[AT]TC
Tsp509I 838 AATT ^AATT
TthHB8I 31 TCGA T^CGA
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwI 750 GATCC GGATC(4/5)
431 GGATC
Asp700I 216 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
315 GAAAACCTTC
AspHI 605 GAGCAC G[AT]GC[AT]^C
738 GTGCTC
AvaI 115 CCCGAG C^[CT]CG[GA]G
211 CCCGAG
AvaII 402 GGACC G^G[AT]CC
591 GGACC
BanI 41 GGCGCC G^G[CT][GA]CC
706 GGCGCC
BbeI 41 GGCGCC GGCGC^C
706 GGCGCC
BmyI 605 GAGCAC G[AGT]GC[ACT]^C
738 GTGCTC
BsaI 172 GAGACC GGTCTC(1/5)
727 GAGACC
BsiHKAI 605 GAGCAC G[AT]GC[AT]^C
738 GTGCTC
BsmBI 534 CGTCTC CGTCTC(1/5)
291 GAGACG
BsmFI 140 GGGAC GGGAC(10/14)
396 GTCCC
BsoBI 115 CCCGAG C^[CT]CG[GA]G
211 CCCGAG
Bsp1286I 605 GAGCAC G[AGT]GC[ACT]^C
738 GTGCTC
BspMI 404 ACCTGC ACCTGC(4/8)
476 ACCTGC
BstYI 371 AGATCT [GA]^GATC[CT]
749 AGATCC
CfoI 42 GCGC GCG^C
707 GCGC
FauI 760 CCCGC CCCGC(4/6)
833 CCCGC
HaeII 41 GGCGCC [GA]GCGC^[CT]
706 GGCGCC
HgaI 293 GACGC GACGC(5/10)
533 GCGTC
HhaI 42 GCGC GCG^C
707 GCGC
HinP1I 42 GCGC G^CGC
707 GCGC
HinfI 28 GACTC G^A[ACGT]TC
423 GATTC
KasI 41 GGCGCC G^GCGCC
706 GGCGCC
MflI 371 AGATCT [GA]^GATC[CT]
749 AGATCC
MslI 241 CACGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
444 CACAGATGTG
NarI 41 GGCGCC GG^CGCC
706 GGCGCC
SfaNI 254 GCATC GCATC(5/9)
263 GCATC
SfoI 41 GGCGCC GGC^GCC
706 GGCGCC
TspRI 324 CACTG CA[GC]TG[ACGT][ACGT]^
795 CAGTG
XcmI 644 CCAAGCGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
710 CCATGATGAGTGTGG
XhoII 371 AGATCT [GA]^GATC[CT]
749 AGATCC
XmnI 216 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
315 GAAAACCTTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AcyI AfaI AluI BbvI
BsaHI BsaJI BsiYI BslI BsmAI
BssKI BstNI Cac8I Cfr13I DpnII
DsaI EaeI EcoRII Fnu4HI HaeIII
Hin1I HphI ItaI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TseI Tsp45I