(Circular) (Six-base) MAPSORT of: pFN21AB6426.seq Check: 6096 from: 1 to: 5563 pFN21AB6426 5563 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3373 278 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2906 1937 Size: 969 4594 Acc65I G'GTAC_C Cuts at: 2884 2884 Size: 5563 AclI AA'CG_TT Cuts at: 1563 3690 4063 1563 Size: 2127 373 3063 Fragments arranged by size: 3063 2127 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3626 5010 5278 1632 Size: 1994 1384 268 1917 Fragments arranged by size: 1994 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5366 AflIII A'CryG_T Cuts at: 1203 4462 5439 1203 Size: 3259 977 1327 Fragments arranged by size: 3259 1327 977 AgeI A'CCGG_T Cuts at: 4394 4394 Size: 5563 AhdI GACnn_n'nnGTC Cuts at: 2413 4292 2413 Size: 1879 3684 AleI CACnn'nnGTG Cuts at: 1166 2250 2453 1166 Size: 1084 203 4276 Fragments arranged by size: 4276 1084 203 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5563 AlwNI CAG_nnn'CTG Cuts at: 2470 3306 4878 2470 Size: 836 1572 3155 Fragments arranged by size: 3155 1572 836 ApaLI G'TGCA_C Cuts at: 3620 4776 3620 Size: 1156 4407 ApoI r'AATT_y Cuts at: 1493 2872 3098 3250 5359 1493 Size: 1379 226 152 2109 1697 Fragments arranged by size: 2109 1697 1379 226 152 AseI AT'TA_AT Cuts at: 160 1032 4117 160 Size: 872 3085 1606 Fragments arranged by size: 3085 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 AvaI C'yCGr_G Cuts at: 1870 1943 2120 2216 2888 1870 Size: 73 177 96 672 4545 Fragments arranged by size: 4545 672 177 96 73 BamHI G'GATC_C Cuts at: 2893 3260 2893 Size: 367 5196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2046 2711 2884 4339 618 Size: 325 217 627 259 665 173 1455 1842 Fragments arranged by size: 1842 1455 665 627 325 259 217 173 BanII G_rGCy'C Cuts at: 1391 2882 1391 Size: 1491 4072 BbeI G_GCGC'C Cuts at: 2050 2715 2050 Size: 665 4898 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2634 928 Size: 971 735 3857 Fragments arranged by size: 3857 971 735 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2627 2661 3754 3788 1903 Size: 34 690 34 1093 34 3678 Fragments arranged by size: 3678 1093 690 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3455 4665 3455 Size: 1210 4353 BclI T'GATC_A Cuts at: 1548 1548 Size: 5563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2394 4174 136 Size: 107 122 71 631 605 722 1780 1525 Fragments arranged by size: 1780 1525 722 631 605 122 107 71 BglII A'GATC_T Cuts at: 2376 5558 2376 Size: 3182 2381 BlpI GC'TnA_GC Cuts at: 2980 2980 Size: 5563 Bme1580I G_kGCm'C Cuts at: 1790 3624 4780 1790 Size: 1834 1156 2573 Fragments arranged by size: 2573 1834 1156 BmgBI CAC'GTC Cuts at: 1318 2509 1318 Size: 1191 4372 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3294 4252 442 Size: 416 53 83 399 24 1877 958 1753 Fragments arranged by size: 1877 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5563 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2835 4223 1239 Size: 1596 1388 2579 Fragments arranged by size: 2579 1596 1388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 3688 4553 4851 2744 Size: 944 865 298 3456 Fragments arranged by size: 3456 944 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2171 2726 4226 882 Size: 617 672 555 1500 2219 Fragments arranged by size: 2219 1500 672 617 555 BsaAI yAC'GTr Cuts at: 493 1204 3354 493 Size: 711 2150 2702 Fragments arranged by size: 2702 2150 711 BsaBI GATnn'nnATC Cuts at: 5557 5557 Size: 5563 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2047 2538 2712 3370 Size: 53 83 186 936 514 491 174 658 Cuts at: 3370 3752 4387 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 658 635 514 491 382 186 174 83 53 BsaWI w'CCGG_w Cuts at: 3996 4394 4668 4815 5226 3996 Size: 398 274 147 411 4333 Fragments arranged by size: 4333 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2547 2577 1178 Size: 30 165 30 1144 30 4164 Fragments arranged by size: 4164 1144 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5563 BseYI C'CCAG_C Cuts at: 2517 4766 5262 2517 Size: 2249 496 2818 Fragments arranged by size: 2818 2249 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2531 1163 Size: 573 795 4195 Fragments arranged by size: 4195 795 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2533 3013 3774 4802 1540 Size: 275 186 532 480 761 1028 2301 Fragments arranged by size: 2301 1028 761 532 480 275 186 BsiHKAI G_wGCw'C Cuts at: 2614 2747 3624 3709 4780 2614 Size: 133 877 85 1071 3397 Fragments arranged by size: 3397 1071 877 133 85 BsmI GAATG_Cn' Cuts at: 3074 3167 3074 Size: 93 5470 BsmBI CGTCTCn'nnnn_ Cuts at: 2290 2545 2290 Size: 255 5308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2614 2747 2882 3624 3709 4780 1391 Size: 399 824 133 135 742 85 1071 2174 Fragments arranged by size: 2174 1071 824 742 399 135 133 85 BspEI T'CCGG_A Cuts at: 5226 5226 Size: 5563 BspHI T'CATG_A Cuts at: 3452 5182 3452 Size: 1730 3833 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2418 2490 2918 844 Size: 1574 72 428 3489 Fragments arranged by size: 3489 1574 428 72 BsrBI CCG'CTC Cuts at: 1394 1823 3450 1394 Size: 429 1627 3507 Fragments arranged by size: 3507 1627 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4058 4232 65 Size: 1183 2810 174 1396 Fragments arranged by size: 2810 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4207 4394 1700 Size: 110 2397 187 2869 Fragments arranged by size: 2869 2397 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5563 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5563 BssSI C'ACGA_G Cuts at: 2246 3623 4635 2246 Size: 1377 1012 3174 Fragments arranged by size: 3174 1377 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5563 BstBI TT'CG_AA Cuts at: 4367 4367 Size: 5563 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5563 BstYI r'GATC_y Cuts at: 1972 2376 2754 2893 3260 3645 3662 5114 5200 Size: 404 378 139 367 385 17 1452 86 Cuts at: 5200 5212 5558 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 404 385 378 367 346 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2236 2389 2533 513 Size: 552 1171 153 144 3543 Fragments arranged by size: 3543 1171 552 153 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2338 487 Size: 683 56 1112 3712 Fragments arranged by size: 3712 1112 683 56 BtsI GCAGTG_nn' Cuts at: 3079 3873 3893 3079 Size: 794 20 4749 Fragments arranged by size: 4749 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2671 2706 529 Size: 35 2107 35 3386 Fragments arranged by size: 3386 2107 35 35 DraI TTT'AAA Cuts at: 2867 2867 Size: 5563 DraIII CAC_nnn'GTG Cuts at: 2620 2620 Size: 5563 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2537 4570 809 Size: 507 1221 2033 1802 Fragments arranged by size: 2033 1802 1221 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 2358 2568 Size: 54 1425 50 275 18 241 287 210 Cuts at: 2568 3010 3899 5528 8 Size: 442 889 1629 43 Fragments arranged by size: 1629 1425 889 442 287 275 241 210 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3010 1537 Size: 275 1198 4090 Fragments arranged by size: 4090 1198 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3493 1353 Size: 2140 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2449 2836 4150 4524 4670 387 Size: 1064 138 860 387 1314 374 146 1280 Fragments arranged by size: 1314 1280 1064 860 387 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2835 3626 4223 5010 5278 1239 Size: 393 1203 791 597 787 268 1524 Fragments arranged by size: 1524 1203 791 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2467 2467 Size: 5563 EcoRI G'AATT_C Cuts at: 2872 3250 2872 Size: 378 5185 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2188 2608 2188 Size: 420 5143 FspI TGC'GCA Cuts at: 4069 4069 Size: 5563 HaeII r_GCGC'y Cuts at: 1678 2050 2715 4710 1678 Size: 372 665 1995 2531 Fragments arranged by size: 2531 1995 665 372 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 4204 4236 4278 Size: 32 135 32 827 32 1773 32 42 Cuts at: 4278 4310 1373 Size: 32 2626 Fragments arranged by size: 2626 1773 827 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2907 3153 669 Size: 988 281 969 246 3079 Fragments arranged by size: 3079 988 969 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5563 HpaI GTT'AAC Cuts at: 3153 3153 Size: 5563 KasI G'GCGC_C Cuts at: 2046 2711 2046 Size: 665 4898 KpnI G_GTAC'C Cuts at: 2888 2888 Size: 5563 MfeI C'AATT_G Cuts at: 3162 3162 Size: 5563 MluI A'CGCG_T Cuts at: 5439 5439 Size: 5563 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2034 4677 4861 1305 Size: 729 2643 184 2007 Fragments arranged by size: 2643 2007 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 10 Size: 54 1425 343 738 3003 Fragments arranged by size: 3003 1425 738 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2250 2453 3521 3880 4039 518 Size: 648 1084 203 1068 359 159 2042 Fragments arranged by size: 2042 1084 1068 648 359 203 159 MspA1I CmG'CkG Cuts at: 1606 2659 2979 3656 4804 5049 1606 Size: 1053 320 677 1148 245 2120 Fragments arranged by size: 2120 1148 1053 677 320 245 NarI GG'CG_CC Cuts at: 2047 2712 2047 Size: 665 4898 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5011 NdeI CA'TA_TG Cuts at: 387 387 Size: 5563 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5563 NotI GC'GGCC_GC Cuts at: 3010 3010 Size: 5563 NspI r_CATG'y Cuts at: 1132 1229 2921 4466 1132 Size: 97 1692 1545 2229 Fragments arranged by size: 2229 1692 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5563 PciI A'CATG_T Cuts at: 4462 4462 Size: 5563 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5563 PmeI GTTT'AAAC Cuts at: 2867 2867 Size: 5563 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3632 3664 5145 5177 3632 Size: 32 1481 32 4018 Fragments arranged by size: 4018 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5563 PsiI TTA'TAA Cuts at: 3133 3133 Size: 5563 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2780 2812 2780 Size: 32 5531 PstI C_TGCA'G Cuts at: 830 2415 2915 830 Size: 1585 500 3478 Fragments arranged by size: 3478 1585 500 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5563 PvuII CAG'CTG Cuts at: 2659 2659 Size: 5563 SalI G'TCGA_C Cuts at: 1936 2905 1936 Size: 969 4594 SbfI CC_TGCA'GG Cuts at: 2915 2915 Size: 5563 ScaI AGT'ACT Cuts at: 2078 3811 5523 2078 Size: 1733 1712 2118 Fragments arranged by size: 2118 1733 1712 SexAI A'CCwGG_T Cuts at: 2616 2616 Size: 5563 SfcI C'TryA_G Cuts at: 826 1045 2411 2911 4046 4727 4918 826 Size: 219 1366 500 1135 681 191 1471 Fragments arranged by size: 1471 1366 1135 681 500 219 191 SfoI GGC'GCC Cuts at: 2048 2713 2048 Size: 665 4898 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5563 SmaI CCC'GGG Cuts at: 1872 2890 1872 Size: 1018 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2759 3667 4568 4830 820 Size: 197 926 816 908 901 262 1553 Fragments arranged by size: 1553 926 908 901 816 262 197 SnaBI TAC'GTA Cuts at: 493 3354 493 Size: 2861 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5563 SphI G_CATG'C Cuts at: 1132 2921 1132 Size: 1789 3774 SspI AAT'ATT Cuts at: 5 52 3487 5 Size: 47 3435 2081 Fragments arranged by size: 3435 2081 47 StuI AGG'CCT Cuts at: 1517 2397 1517 Size: 880 4683 StyI C'CwwG_G Cuts at: 513 1065 2172 3002 513 Size: 552 1107 830 3074 Fragments arranged by size: 3074 1107 830 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3590 3607 3760 3945 4390 325 Size: 1255 2010 17 153 185 445 1498 Fragments arranged by size: 2010 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2076 3809 5521 Size: 274 80 33 51 1443 99 1733 1712 Cuts at: 5521 96 Size: 138 Fragments arranged by size: 1733 1712 1443 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2547 2579 5523 5555 2547 Size: 32 2944 32 2555 Fragments arranged by size: 2944 2555 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5563 XbaI T'CTAG_A Cuts at: 2899 2899 Size: 5563 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2656 2722 2656 Size: 66 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5563 XmaI C'CCGG_G Cuts at: 1870 2888 1870 Size: 1018 4545 XmnI GAAnn'nnTTC Cuts at: 2225 2324 3692 2225 Size: 99 1368 4096 Fragments arranged by size: 4096 1368 99 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3371 276 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI