Restriction map for
pFN21AB6426
(Linear) (Six-base) MAPSORT of: KIBB6426.seq Check: 8326 from: 1 to: 858
KIBB6426.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 408 858
Size: 408 450
AleI CACnn'nnGTG
Cuts at: 0 245 448 858
Size: 245 203 410
Fragments arranged by size:
410 245 203
AlwNI CAG_nnn'CTG
Cuts at: 0 465 858
Size: 465 393
AvaI C'yCGr_G
Cuts at: 0 115 211 858
Size: 115 96 647
Fragments arranged by size:
647 115 96
BanI G'GyrC_C
Cuts at: 0 41 706 858
Size: 41 665 152
Fragments arranged by size:
665 152 41
BbeI G_GCGC'C
Cuts at: 0 45 710 858
Size: 45 665 148
Fragments arranged by size:
665 148 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 629 858
Size: 629 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 622 656 858
Size: 622 34 202
Fragments arranged by size:
622 202 34
BglI GCCn_nnn'nGGC
Cuts at: 0 389 858
Size: 389 469
BglII A'GATC_T
Cuts at: 0 371 858
Size: 371 487
BmgBI CAC'GTC
Cuts at: 0 504 858
Size: 504 354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 858
Size: 830 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 858
Size: 739 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 721 858
Size: 166 555 137
Fragments arranged by size:
555 166 137
BsaHI Gr'CG_yC
Cuts at: 0 42 533 707 858
Size: 42 491 174 151
Fragments arranged by size:
491 174 151 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 542 572 858
Size: 542 30 286
Fragments arranged by size:
542 286 30
BseYI C'CCAG_C
Cuts at: 0 512 858
Size: 512 346
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 858
Size: 526 332
BsiEI CG_ry'CG
Cuts at: 0 528 858
Size: 528 330
BsiHKAI G_wGCw'C
Cuts at: 0 609 742 858
Size: 609 133 116
Fragments arranged by size:
609 133 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 285 540 858
Size: 285 255 318
Fragments arranged by size:
318 285 255
Bsp1286I G_dGCh'C
Cuts at: 0 609 742 858
Size: 609 133 116
Fragments arranged by size:
609 133 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 413 485 858
Size: 413 72 373
Fragments arranged by size:
413 373 72
BssSI C'ACGA_G
Cuts at: 0 241 858
Size: 241 617
BstYI r'GATC_y
Cuts at: 0 371 749 858
Size: 371 378 109
Fragments arranged by size:
378 371 109
BtgI C'CryG_G
Cuts at: 0 231 384 528 858
Size: 231 153 144 330
Fragments arranged by size:
330 231 153 144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 333 858
Size: 333 525
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 666 701 858
Size: 666 35 157
Fragments arranged by size:
666 157 35
DraIII CAC_nnn'GTG
Cuts at: 0 615 858
Size: 615 243
DrdI GACnn_nn'nnGTC
Cuts at: 0 532 858
Size: 532 326
EaeI y'GGCC_r
Cuts at: 0 66 353 563 858
Size: 66 287 210 295
Fragments arranged by size:
295 287 210 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 444 831 858
Size: 444 387 27
Fragments arranged by size:
444 387 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 858
Size: 830 28
EcoO109I rG'GnC_Cy
Cuts at: 0 462 858
Size: 462 396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 603 858
Size: 183 420 255
Fragments arranged by size:
420 255 183
HaeII r_GCGC'y
Cuts at: 0 45 710 858
Size: 45 665 148
Fragments arranged by size:
665 148 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 394 426 858
Size: 394 32 432
Fragments arranged by size:
432 394 32
KasI G'GCGC_C
Cuts at: 0 41 706 858
Size: 41 665 152
Fragments arranged by size:
665 152 41
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 858
Size: 29 829
MscI TGG'CCA
Cuts at: 0 565 858
Size: 565 293
MslI CAynn'nnrTG
Cuts at: 0 245 448 858
Size: 245 203 410
Fragments arranged by size:
410 245 203
MspA1I CmG'CkG
Cuts at: 0 654 858
Size: 654 204
NarI GG'CG_CC
Cuts at: 0 42 707 858
Size: 42 665 151
Fragments arranged by size:
665 151 42
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 775 807 858
Size: 775 32 51
Fragments arranged by size:
775 51 32
PstI C_TGCA'G
Cuts at: 0 410 858
Size: 410 448
PvuII CAG'CTG
Cuts at: 0 654 858
Size: 654 204
ScaI AGT'ACT
Cuts at: 0 73 858
Size: 73 785
SexAI A'CCwGG_T
Cuts at: 0 611 858
Size: 611 247
SfcI C'TryA_G
Cuts at: 0 406 858
Size: 406 452
SfoI GGC'GCC
Cuts at: 0 43 708 858
Size: 43 665 150
Fragments arranged by size:
665 150 43
SmlI C'TyrA_G
Cuts at: 0 754 858
Size: 754 104
StuI AGG'CCT
Cuts at: 0 392 858
Size: 392 466
StyI C'CwwG_G
Cuts at: 0 167 858
Size: 167 691
TatI w'GTAC_w
Cuts at: 0 71 858
Size: 71 787
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 542 574 858
Size: 542 32 284
Fragments arranged by size:
542 284 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 651 717 858
Size: 651 66 141
Fragments arranged by size:
651 141 66
XmnI GAAnn'nnTTC
Cuts at: 0 220 319 858
Size: 220 99 539
Fragments arranged by size:
539 220 99
Enzymes that do cut:
AhdI AleI AlwNI AvaI BanI BbeI BbsI BcgI
BglI BglII BmgBI BpmI BpuEI BsaI BsaHI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BssSI
BstYI BtgI BtgZI CspCI DraIII DrdI EaeI EciI
Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI PsrI PstI PvuII ScaI SexAI
SfcI SfoI SmlI StuI StyI TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbvCI BciVI
BclI BfrBI BlpI Bme1580I BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsaWI BseRI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtsI ClaI DraI EagI EarI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI
XhoI XmaI ZraI