Restriction map for pFN21AB6439
 (Circular) (Six-base) MAPSORT of: pFN21AB6439.seq  Check: 7138  from: 1  to: 5647



pFN21AB6439  5647 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:40  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3457     278 
   Size:         53      83     186     936    1921    2468

  Fragments arranged by size: 

                2468    1921     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2701    2990    1937 
   Size:        764     289    4594

  Fragments arranged by size: 

                4594     764     289
 
Acc65I G'GTAC_C

Cuts at:   2968    2968 
   Size:       5647
 
AclI AA'CG_TT

Cuts at:   1563    3774    4147    1563 
   Size:       2211     373    3063

  Fragments arranged by size: 

                3063    2211     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3710    5094    5362    1632 
   Size:       2078    1384     268    1917

  Fragments arranged by size: 

                2078    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5450
 
AflIII A'CryG_T

Cuts at:   1203    2756    4546    5523    1203 
   Size:       1553    1790     977    1327

  Fragments arranged by size: 

                1790    1553    1327     977
 
AgeI A'CCGG_T

Cuts at:   4478    4478 
   Size:       5647
 
AhdI GACnn_n'nnGTC

Cuts at:   4376    4376 
   Size:       5647
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5647
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2492    1184 
   Size:       1308    4339
 
AlwNI CAG_nnn'CTG

Cuts at:   3390    4962    3390 
   Size:       1572    4075
 
ApaLI G'TGCA_C

Cuts at:   2530    3704    4860    2530 
   Size:       1174    1156    3317

  Fragments arranged by size: 

                3317    1174    1156
 
ApoI r'AATT_y

Cuts at:   1493    2162    2515    2956    3182    3334    5443    1493 
   Size:        669     353     441     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     669     441     353     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4201     160 
   Size:        872    3169    1606

  Fragments arranged by size: 

                3169    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5647
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2972    1870 
   Size:         73    1029    4545

  Fragments arranged by size: 

                4545    1029      73
 
BamHI G'GATC_C

Cuts at:   2876    2977    3344    2876 
   Size:        101     367    5179

  Fragments arranged by size: 

                5179     367     101
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2968    4423     618 
   Size:        325     217     627    1181    1455    1842

  Fragments arranged by size: 

                1842    1455    1181     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2397    2966    1391 
   Size:       1006     569    4072

  Fragments arranged by size: 

                4072    1006     569
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2333     928 
   Size:        971     434    4242

  Fragments arranged by size: 

                4242     971     434
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3838    3872    1903 
   Size:         34    1901      34    3678

  Fragments arranged by size: 

                3678    1901      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3539    4749    3539 
   Size:       1210    4437
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5647
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4258     136 
   Size:        107     122      71     631     605    2586    1525

  Fragments arranged by size: 

                2586    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5642    5642 
   Size:       5647
 
BlpI GC'TnA_GC

Cuts at:   3064    3064 
   Size:       5647
 
Bme1580I G_kGCm'C

Cuts at:   1790    2502    2534    3708    4864    1790 
   Size:        712      32    1174    1156    2573

  Fragments arranged by size: 

                2573    1174    1156     712      32
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5647
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2581    2640    2920 
   Size:        416      53      83     399      24    1164      59     280

Cuts at:   2920    3378    4336     442 
   Size:        458     958    1753

  Fragments arranged by size: 

                1753    1164     958     458     416     399     280      83
                  59      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5647
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4307    1239 
   Size:       3068    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2666    3772    4637    4935    2666 
   Size:       1106     865     298    3378

  Fragments arranged by size: 

                3378    1106     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2372    4310     882 
   Size:        617     873    1938    2219

  Fragments arranged by size: 

                2219    1938     873     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2032    3438     493 
   Size:        711     828    1406    2702

  Fragments arranged by size: 

                2702    1406     828     711
 
BsaBI GATnn'nnATC

Cuts at:   5641    5641 
   Size:       5647
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3454    3836    4471     275 
   Size:         53      83     186     936    1921     382     635    1451

  Fragments arranged by size: 

                1921    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4080    4478    4752    4899    5310    4080 
   Size:        398     274     147     411    4417

  Fragments arranged by size: 

                4417     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5422

  Fragments arranged by size: 

                5422     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5647
 
BseYI C'CCAG_C

Cuts at:   4850    5346    4850 
   Size:        496    5151
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5074
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3097    3858    4886    1540 
   Size:        275     186    1096     761    1028    2301

  Fragments arranged by size: 

                2301    1096    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2534    3708    3793    4864    2534 
   Size:       1174      85    1071    3317

  Fragments arranged by size: 

                3317    1174    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3158    3251    3158 
   Size:         93    5554
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2397    2502    2534    2966    3708    3793    4864 
   Size:        399     607     105      32     432     742      85    1071

Cuts at:   4864    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     607     432     399     105      85
                  32
 
BspEI T'CCGG_A

Cuts at:   5310    5310 
   Size:       5647
 
BspHI T'CATG_A

Cuts at:   2536    3536    5266    2536 
   Size:       1000    1730    2917

  Fragments arranged by size: 

                2917    1730    1000
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3002     844 
   Size:       2158    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2415    3534    1394 
   Size:        429     592    1119    3507

  Fragments arranged by size: 

                3507    1119     592     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2397    4142    4316      65 
   Size:       1183    1149    1745     174    1396

  Fragments arranged by size: 

                1745    1396    1183    1149     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2458    4291    4478    1700 
   Size:        110     648    1833     187    2869

  Fragments arranged by size: 

                2869    1833     648     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    2430      96 
   Size:       2334    3313
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5647
 
BssSI C'ACGA_G

Cuts at:   3707    4719    3707 
   Size:       1012    4635
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2180     794 
   Size:       1386    4261
 
BstBI TT'CG_AA

Cuts at:   4451    4451 
   Size:       5647
 
BstEII G'GTnAC_C

Cuts at:   1184    2806    1184 
   Size:       1622    4025
 
BstYI r'GATC_y

Cuts at:   1972    2876    2977    3344    3729    3746    5198    5284    5296 
   Size:        904     101     367     385      17    1452      86      12

Cuts at:   5296    5642    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     904     385     367     346     101      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2424    2424 
   Size:       5647
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2709     513 
   Size:        552     939     705    3451

  Fragments arranged by size: 

                3451     939     705     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4908

  Fragments arranged by size: 

                4908     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3163    3957    3977    3163 
   Size:        794      20    4833

  Fragments arranged by size: 

                4833     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5647
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5612
 
DraI TTT'AAA

Cuts at:   2951    2951 
   Size:       5647
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4654     809 
   Size:        507    3338    1802

  Fragments arranged by size: 

                3338    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2007    2247    2464 
   Size:         54    1425      50     275      18     177     240     217

Cuts at:   2464    2712    2896    3094    3983    5612       8 
   Size:        248     184     198     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     275     248     240     217     198
                 184     177      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3094    1537 
   Size:        275    1282    4090

  Fragments arranged by size: 

                4090    1282     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2281    2331    3577    1353 
   Size:        928      50    1246    3423

  Fragments arranged by size: 

                3423    1246     928      50
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4234    4608    4754     387 
   Size:       1064     138    2645     374     146    1280

  Fragments arranged by size: 

                2645    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3710    4307    5094    5362    1239 
   Size:        393    2078     597     787     268    1524

  Fragments arranged by size: 

                2078    1524     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2956    3334    2956 
   Size:        378    5269
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2041    2041 
   Size:       5647
 
FspI TGC'GCA

Cuts at:   4153    4153 
   Size:       5647
 
HaeII r_GCGC'y

Cuts at:   1678    4794    1678 
   Size:       3116    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4288    4320    4362    4394    1373 
   Size:         32     135      32    2716      32      42      32    2626

  Fragments arranged by size: 

                2716    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2991    3237     669 
   Size:        988     281    1053     246    3079

  Fragments arranged by size: 

                3079    1053     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5647
 
HpaI GTT'AAC

Cuts at:   3237    3237 
   Size:       5647
 
KpnI G_GTAC'C

Cuts at:   2972    2972 
   Size:       5647
 
MfeI C'AATT_G

Cuts at:   3246    3246 
   Size:       5647
 
MluI A'CGCG_T

Cuts at:   5523    5523 
   Size:       5647
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4761    4945    1305 
   Size:       3456     184    2007

  Fragments arranged by size: 

                3456    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2009    2249    2714    2898      10 
   Size:         54    1425     343     177     240     465     184    2759

  Fragments arranged by size: 

                2759    1425     465     343     240     184     177      54
            
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2474    3605    3964    4123     518 
   Size:        648    1308    1131     359     159    2042

  Fragments arranged by size: 

                2042    1308    1131     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2523    3063    3740    4888    5133    1606 
   Size:        917     540     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     917     677     540     245
 
NaeI GCC'GGC

Cuts at:   2460    2460 
   Size:       5647
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2709     513 
   Size:        552     939     705    3451

  Fragments arranged by size: 

                3451     939     705     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5647
 
NgoMIV G'CCGG_C

Cuts at:   2458    2458 
   Size:       5647
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5647
 
NotI GC'GGCC_GC

Cuts at:   3094    3094 
   Size:       5647
 
NspI r_CATG'y

Cuts at:   1132    1229    2760    2792    3005    4550    1132 
   Size:         97    1531      32     213    1545    2229

  Fragments arranged by size: 

                2229    1545    1531     213      97      32
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5647
 
PciI A'CATG_T

Cuts at:   2756    4546    2756 
   Size:       1790    3857
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5647
 
PmeI GTTT'AAAC

Cuts at:   2951    2951 
   Size:       5647
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3716    3748    5229    5261    3716 
   Size:         32    1481      32    4102

  Fragments arranged by size: 

                4102    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5647
 
PsiI TTA'TAA

Cuts at:   3217    3217 
   Size:       5647
 
PstI C_TGCA'G

Cuts at:    830    2188    2999     830 
   Size:       1358     811    3478

  Fragments arranged by size: 

                3478    1358     811
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5647
 
SalI G'TCGA_C

Cuts at:   1936    2989    1936 
   Size:       1053    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2281    2281 
   Size:       5647
 
SbfI CC_TGCA'GG

Cuts at:   2999    2999 
   Size:       5647
 
ScaI AGT'ACT

Cuts at:   3895    5607    3895 
   Size:       1712    3935
 
SexAI A'CCwGG_T

Cuts at:   2695    2695 
   Size:       5647
 
SfcI C'TryA_G

Cuts at:    826    1045    2184    2995    4130    4811    5002     826 
   Size:        219    1139     811    1135     681     191    1471

  Fragments arranged by size: 

                1471    1139    1135     811     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5647
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5647
 
SmaI CCC'GGG

Cuts at:   1872    2974    1872 
   Size:       1102    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2681    3751    4652    4914     820 
   Size:        197     926     738    1070     901     262    1553

  Fragments arranged by size: 

                1553    1070     926     901     738     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3438     493 
   Size:       2945    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5647
 
SphI G_CATG'C

Cuts at:   1132    3005    1132 
   Size:       1873    3774
 
SspI AAT'ATT

Cuts at:      5      52    3571       5 
   Size:         47    3519    2081

  Fragments arranged by size: 

                3519    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2817    1517 
   Size:       1300    4347
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2709    2856    3086     513 
   Size:        552     939     705     147     230    3074

  Fragments arranged by size: 

                3074     939     705     552     230     147
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2024    3674    3691    3844    4029    4474     325 
   Size:       1255     444    1650      17     153     185     445    1498

  Fragments arranged by size: 

                1650    1498    1255     445     444     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2430    3893    5605 
   Size:        274      80      33      51    1443     453    1463    1712

Cuts at:   5605      96 
   Size:        138

  Fragments arranged by size: 

                1712    1463    1443     453     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5607    5639    5607 
   Size:         32    5615
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2697    1316 
   Size:       1381    4266
 
XbaI T'CTAG_A

Cuts at:   2983    2983 
   Size:       5647
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2627    2627 
   Size:       5647
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5647
 
XmaI C'CCGG_G

Cuts at:   1870    2972    1870 
   Size:       1102    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2657    3776    2657 
   Size:       1119    4528
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3455     276 
   Size:         53      83     186     936    1921    2468

  Fragments arranged by size: 

                2468    1921     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI
     SapI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI
     NarI     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI     SfiI
     SfoI     SrfI     SwaI
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