(Circular) (Six-base) MAPSORT of: pFN21AE0475.seq Check: 8285 from: 1 to: 5461 pFN21AE0475 5461 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3271 278 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2562 2804 1937 Size: 625 242 4594 Fragments arranged by size: 4594 625 242 Acc65I G'GTAC_C Cuts at: 2782 2782 Size: 5461 AclI AA'CG_TT Cuts at: 1563 3588 3961 1563 Size: 2025 373 3063 Fragments arranged by size: 3063 2025 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3524 4908 5176 1632 Size: 1892 1384 268 1917 Fragments arranged by size: 1917 1892 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5264 AflIII A'CryG_T Cuts at: 1203 4360 5337 1203 Size: 3157 977 1327 Fragments arranged by size: 3157 1327 977 AgeI A'CCGG_T Cuts at: 4292 4292 Size: 5461 AhdI GACnn_n'nnGTC Cuts at: 2558 4190 2558 Size: 1632 3829 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2170 1184 Size: 986 4475 AlwNI CAG_nnn'CTG Cuts at: 3204 4776 3204 Size: 1572 3889 ApaLI G'TGCA_C Cuts at: 3518 4674 3518 Size: 1156 4305 ApoI r'AATT_y Cuts at: 1493 2281 2718 2770 2996 3148 5257 1493 Size: 788 437 52 226 152 2109 1697 Fragments arranged by size: 2109 1697 788 437 226 152 52 AseI AT'TA_AT Cuts at: 160 1032 4015 160 Size: 872 2983 1606 Fragments arranged by size: 2983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 AvaI C'yCGr_G Cuts at: 1870 1943 2097 2786 1870 Size: 73 154 689 4545 Fragments arranged by size: 4545 689 154 73 BamHI G'GATC_C Cuts at: 2791 3158 2791 Size: 367 5094 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 2782 4237 618 Size: 325 217 627 238 757 1455 1842 Fragments arranged by size: 1842 1455 757 627 325 238 217 BanII G_rGCy'C Cuts at: 1391 2058 2780 1391 Size: 667 722 4072 Fragments arranged by size: 4072 722 667 BbeI G_GCGC'C Cuts at: 2029 2029 Size: 5461 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2369 928 Size: 971 470 4020 Fragments arranged by size: 4020 971 470 BbvCI CC'TCA_GC Cuts at: 2066 2066 Size: 5461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3652 3686 1903 Size: 34 1715 34 3678 Fragments arranged by size: 3678 1715 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4563 3353 Size: 1210 4251 BclI T'GATC_A Cuts at: 1548 1548 Size: 5461 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4072 136 Size: 107 122 71 631 605 2400 1525 Fragments arranged by size: 2400 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2683 5456 2683 Size: 2773 2688 BlpI GC'TnA_GC Cuts at: 2878 2878 Size: 5461 Bme1580I G_kGCm'C Cuts at: 1790 2189 3522 4678 1790 Size: 399 1333 1156 2573 Fragments arranged by size: 2573 1333 1156 399 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5461 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2282 3192 4150 Size: 416 53 83 399 24 865 910 958 Cuts at: 4150 442 Size: 1753 Fragments arranged by size: 1753 958 910 865 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5461 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2595 4121 1239 Size: 1356 1526 2579 Fragments arranged by size: 2579 1526 1356 Bpu10I CC'TnA_GC Cuts at: 2066 2066 Size: 5461 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3586 4451 4749 3586 Size: 865 298 4298 Fragments arranged by size: 4298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2129 2295 4124 882 Size: 617 630 166 1829 2219 Fragments arranged by size: 2219 1829 630 617 166 BsaAI yAC'GTr Cuts at: 493 1204 3252 493 Size: 711 2048 2702 Fragments arranged by size: 2702 2048 711 BsaBI GATnn'nnATC Cuts at: 2682 5455 2682 Size: 2773 2688 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2026 3268 3650 4285 Size: 53 83 186 936 493 1242 382 635 Cuts at: 4285 275 Size: 1451 Fragments arranged by size: 1451 1242 936 635 493 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2498 3894 4292 4566 4713 5124 2498 Size: 1396 398 274 147 411 2835 Fragments arranged by size: 2835 1396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5236 Fragments arranged by size: 5236 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2666 1186 Size: 907 573 3981 Fragments arranged by size: 3981 907 573 BseYI C'CCAG_C Cuts at: 2626 4664 5160 2626 Size: 2038 496 2927 Fragments arranged by size: 2927 2038 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2166 2418 1163 Size: 573 430 252 4206 Fragments arranged by size: 4206 573 430 252 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2911 3672 4700 1540 Size: 275 186 910 761 1028 2301 Fragments arranged by size: 2301 1028 910 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2743 3522 3607 4678 2743 Size: 779 85 1071 3526 Fragments arranged by size: 3526 1071 779 85 BsmI GAATG_Cn' Cuts at: 2972 3065 2972 Size: 93 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2189 2743 2780 3522 3607 4678 Size: 399 268 131 554 37 742 85 1071 Cuts at: 4678 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 554 399 268 131 85 37 BspEI T'CCGG_A Cuts at: 5124 5124 Size: 5461 BspHI T'CATG_A Cuts at: 3350 5080 3350 Size: 1730 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2548 2816 844 Size: 1704 268 3489 Fragments arranged by size: 3489 1704 268 BsrBI CCG'CTC Cuts at: 1394 1823 3348 1394 Size: 429 1525 3507 Fragments arranged by size: 3507 1525 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3956 4130 65 Size: 1183 2708 174 1396 Fragments arranged by size: 2708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4105 4292 1700 Size: 110 2295 187 2869 Fragments arranged by size: 2869 2295 187 110 BsrGI T'GTAC_A Cuts at: 96 2311 96 Size: 2215 3246 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5461 BssSI C'ACGA_G Cuts at: 3521 4533 3521 Size: 1012 4449 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5461 BstBI TT'CG_AA Cuts at: 4265 4265 Size: 5461 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5461 BstYI r'GATC_y Cuts at: 1972 2398 2683 2791 3158 3543 3560 5012 5098 Size: 426 285 108 367 385 17 1452 86 Cuts at: 5098 5110 5456 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 426 385 367 346 285 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2130 2594 513 Size: 552 939 126 464 3380 Fragments arranged by size: 3380 939 552 464 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4722 Fragments arranged by size: 4722 683 56 BtsI GCAGTG_nn' Cuts at: 2149 2340 2435 2977 3771 3791 2149 Size: 191 95 542 794 20 3819 Fragments arranged by size: 3819 794 542 191 95 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5461 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5426 DraI TTT'AAA Cuts at: 2765 2765 Size: 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4468 809 Size: 507 3152 1802 Fragments arranged by size: 3152 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 3797 5426 Size: 54 1425 50 275 18 1078 889 1629 Cuts at: 5426 8 Size: 43 Fragments arranged by size: 1629 1425 1078 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2908 1537 Size: 275 1096 4090 Fragments arranged by size: 4090 1096 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2594 3391 1353 Size: 1241 797 3423 Fragments arranged by size: 3423 1241 797 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4048 4422 4568 387 Size: 1064 138 2459 374 146 1280 Fragments arranged by size: 2459 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2595 3524 4121 4908 5176 1239 Size: 393 963 929 597 787 268 1524 Fragments arranged by size: 1524 963 929 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2194 2194 Size: 5461 EcoRI G'AATT_C Cuts at: 2281 2770 3148 2281 Size: 489 378 4594 Fragments arranged by size: 4594 489 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2206 2417 2206 Size: 211 5250 FspI TGC'GCA Cuts at: 3967 3967 Size: 5461 HaeII r_GCGC'y Cuts at: 1678 2029 4608 1678 Size: 351 2579 2531 Fragments arranged by size: 2579 2531 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2544 2576 4102 4134 4176 Size: 32 135 32 972 32 1526 32 42 Cuts at: 4176 4208 1373 Size: 32 2626 Fragments arranged by size: 2626 1526 972 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2563 2805 3051 669 Size: 988 281 625 242 246 3079 Fragments arranged by size: 3079 988 625 281 246 242 HindIII A'AGCT_T Cuts at: 748 748 Size: 5461 HpaI GTT'AAC Cuts at: 3051 3051 Size: 5461 KasI G'GCGC_C Cuts at: 2025 2025 Size: 5461 KpnI G_GTAC'C Cuts at: 2786 2786 Size: 5461 MfeI C'AATT_G Cuts at: 3060 3060 Size: 5461 MluI A'CGCG_T Cuts at: 5337 5337 Size: 5461 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 4575 4759 1305 Size: 687 2583 184 2007 Fragments arranged by size: 2583 2007 687 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3639 Fragments arranged by size: 3639 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3419 3778 3937 518 Size: 648 2253 359 159 2042 Fragments arranged by size: 2253 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2877 3554 4702 4947 1606 Size: 1271 677 1148 245 2120 Fragments arranged by size: 2120 1271 1148 677 245 NarI GG'CG_CC Cuts at: 2026 2026 Size: 5461 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3970 Fragments arranged by size: 3970 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5461 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5461 NotI GC'GGCC_GC Cuts at: 2908 2908 Size: 5461 NspI r_CATG'y Cuts at: 1132 1229 2065 2451 2819 4364 1132 Size: 97 836 386 368 1545 2229 Fragments arranged by size: 2229 1545 836 386 368 97 PacI TTA_AT'TAA Cuts at: 2211 2211 Size: 5461 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5461 PciI A'CATG_T Cuts at: 4360 4360 Size: 5461 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5461 PfoI T'CCnGG_A Cuts at: 2174 2174 Size: 5461 PmeI GTTT'AAAC Cuts at: 2765 2765 Size: 5461 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5043 5075 3530 Size: 32 1481 32 3916 Fragments arranged by size: 3916 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5461 PsiI TTA'TAA Cuts at: 3031 3031 Size: 5461 PstI C_TGCA'G Cuts at: 830 2813 830 Size: 1983 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5461 SalI G'TCGA_C Cuts at: 1936 2561 2803 1936 Size: 625 242 4594 Fragments arranged by size: 4594 625 242 SbfI CC_TGCA'GG Cuts at: 2813 2813 Size: 5461 ScaI AGT'ACT Cuts at: 2084 3709 5421 2084 Size: 1625 1712 2124 Fragments arranged by size: 2124 1712 1625 SfcI C'TryA_G Cuts at: 826 1045 2751 2809 3944 4625 4816 826 Size: 219 1706 58 1135 681 191 1471 Fragments arranged by size: 1706 1471 1135 681 219 191 58 SfoI GGC'GCC Cuts at: 2027 2027 Size: 5461 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5461 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5461 SmaI CCC'GGG Cuts at: 1872 2099 2788 1872 Size: 227 689 4545 Fragments arranged by size: 4545 689 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 3565 4466 4728 820 Size: 197 926 1622 901 262 1553 Fragments arranged by size: 1622 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3252 493 Size: 2759 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5461 SphI G_CATG'C Cuts at: 1132 2451 2819 1132 Size: 1319 368 3774 Fragments arranged by size: 3774 1319 368 SspI AAT'ATT Cuts at: 5 52 2290 3385 5 Size: 47 2238 1095 2081 Fragments arranged by size: 2238 2081 1095 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5461 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 2900 513 Size: 552 939 186 710 3074 Fragments arranged by size: 3074 939 710 552 186 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3488 3505 3658 3843 4288 325 Size: 1255 1908 17 153 185 445 1498 Fragments arranged by size: 1908 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2082 2311 3707 Size: 274 80 33 51 1443 105 229 1396 Cuts at: 3707 5419 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1396 274 229 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5421 5453 5421 Size: 32 5429 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5461 XbaI T'CTAG_A Cuts at: 2797 2797 Size: 5461 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5461 XmaI C'CCGG_G Cuts at: 1870 2097 2786 1870 Size: 227 689 4545 Fragments arranged by size: 4545 689 227 XmnI GAAnn'nnTTC Cuts at: 3590 3590 Size: 5461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3269 276 Size: 53 83 186 936 1735 2468 Fragments arranged by size: 2468 1735 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI