Restriction map for
pFN21AE0475
(Linear) (Six-base) MAPSORT of: KIEE0475.seq Check: 9876 from: 1 to: 756
KIEE0475.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 557 756
Size: 557 199
AhdI GACnn_n'nnGTC
Cuts at: 0 553 756
Size: 553 203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 165 756
Size: 165 591
ApoI r'AATT_y
Cuts at: 0 276 713 756
Size: 276 437 43
Fragments arranged by size:
437 276 43
AvaI C'yCGr_G
Cuts at: 0 92 756
Size: 92 664
BanI G'GyrC_C
Cuts at: 0 20 756
Size: 20 736
BanII G_rGCy'C
Cuts at: 0 53 756
Size: 53 703
BbeI G_GCGC'C
Cuts at: 0 24 756
Size: 24 732
BbsI GAAGACnn'nnnn_
Cuts at: 0 364 756
Size: 364 392
BbvCI CC'TCA_GC
Cuts at: 0 61 756
Size: 61 695
BglII A'GATC_T
Cuts at: 0 678 756
Size: 678 78
Bme1580I G_kGCm'C
Cuts at: 0 184 756
Size: 184 572
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 756
Size: 277 479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 756
Size: 590 166
Bpu10I CC'TnA_GC
Cuts at: 0 61 756
Size: 61 695
BsaI GGTCTCn'nnnn_
Cuts at: 0 124 290 756
Size: 124 166 466
Fragments arranged by size:
466 166 124
BsaBI GATnn'nnATC
Cuts at: 0 677 756
Size: 677 79
BsaHI Gr'CG_yC
Cuts at: 0 21 756
Size: 21 735
BsaWI w'CCGG_w
Cuts at: 0 493 756
Size: 493 263
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 661 756
Size: 88 573 95
Fragments arranged by size:
573 95 88
BseYI C'CCAG_C
Cuts at: 0 621 756
Size: 621 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 413 756
Size: 161 252 343
Fragments arranged by size:
343 252 161
BsiHKAI G_wGCw'C
Cuts at: 0 738 756
Size: 738 18
Bsp1286I G_dGCh'C
Cuts at: 0 53 184 738 756
Size: 53 131 554 18
Fragments arranged by size:
554 131 53 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 543 756
Size: 543 213
BsrGI T'GTAC_A
Cuts at: 0 306 756
Size: 306 450
BstYI r'GATC_y
Cuts at: 0 393 678 756
Size: 393 285 78
Fragments arranged by size:
393 285 78
BtgI C'CryG_G
Cuts at: 0 125 589 756
Size: 125 464 167
Fragments arranged by size:
464 167 125
BtsI GCAGTG_nn'
Cuts at: 0 144 335 430 756
Size: 144 191 95 326
Fragments arranged by size:
326 191 144 95
EarI CTCTTCn'nnn_
Cuts at: 0 589 756
Size: 589 167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 756
Size: 590 166
EcoNI CCTnn'n_nnAGG
Cuts at: 0 189 756
Size: 189 567
EcoRI G'AATT_C
Cuts at: 0 276 756
Size: 276 480
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 201 412 756
Size: 201 211 344
Fragments arranged by size:
344 211 201
HaeII r_GCGC'y
Cuts at: 0 24 756
Size: 24 732
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 539 571 756
Size: 539 32 185
Fragments arranged by size:
539 185 32
HincII GTy'rAC
Cuts at: 0 558 756
Size: 558 198
KasI G'GCGC_C
Cuts at: 0 20 756
Size: 20 736
NarI GG'CG_CC
Cuts at: 0 21 756
Size: 21 735
NspI r_CATG'y
Cuts at: 0 60 446 756
Size: 60 386 310
Fragments arranged by size:
386 310 60
PacI TTA_AT'TAA
Cuts at: 0 206 756
Size: 206 550
PfoI T'CCnGG_A
Cuts at: 0 169 756
Size: 169 587
SalI G'TCGA_C
Cuts at: 0 556 756
Size: 556 200
ScaI AGT'ACT
Cuts at: 0 79 756
Size: 79 677
SfcI C'TryA_G
Cuts at: 0 746 756
Size: 746 10
SfoI GGC'GCC
Cuts at: 0 22 756
Size: 22 734
SmaI CCC'GGG
Cuts at: 0 94 756
Size: 94 662
SphI G_CATG'C
Cuts at: 0 446 756
Size: 446 310
SspI AAT'ATT
Cuts at: 0 285 756
Size: 285 471
StyI C'CwwG_G
Cuts at: 0 185 756
Size: 185 571
TatI w'GTAC_w
Cuts at: 0 77 306 756
Size: 77 229 450
Fragments arranged by size:
450 229 77
XmaI C'CCGG_G
Cuts at: 0 92 756
Size: 92 664
Enzymes that do cut:
AccI AhdI AlfI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BglII Bme1580I BmrI BpmI Bpu10I BsaI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrGI BstYI BtgI BtsI EarI Eco57MI EcoNI
EcoRI FalI HaeII Hin4I HincII KasI NarI NspI
PacI PfoI SalI ScaI SfcI SfoI SmaI SphI
SspI StyI TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SrfI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI